primary root length (mm)
DESCRIPTION
Li and Xue, Supplemental Figure 2. A. 0 1 nM 10 nM 100 nM 1 µ M (IAA). C LO11 LO19. C LO11 LO19. C LO11 LO19. C LO11 LO19. C LO11 LO19. pO-PLD z 2. 0 1 nM 10 nM 100 nM 1 µ M (IAA). - PowerPoint PPT PresentationTRANSCRIPT
B
A
Pri
mar
y ro
ot le
ngt
h (
mm
)
0
5
10
15
20
25
30
35
40
45
Control pO-PLD2-LO11pO-PLD2-LO19pA-PLD2-LA3pA-PLD2-LA11
0 1 nM 10 nM 100 nM 1 µM
IAA concentration
** **
**
**
**
**
0 1 nM 10 nM 100 nM 1 µM (IAA)
C LO11 LO19 C LO11 LO19 C LO11 LO19 C LO11 LO19 C LO11 LO19
0 1 nM 10 nM 100 nM 1 µM (IAA)
pO-PLD2
pA-PLD2
C LA11 LA19 C LA11 LA19 C LA11 LA19 C LA11 LA19 C LA11 LA19
Supplemental Figure 2. Altered Expression of PLD2 Result in Altered Auxin Effects on Primary Root Growth.
A. Compared with controls (C), PLD2-overexpressing plants were hypersensitive to auxin treatment (upper panel), whereas those deficient in were less sensitive (lower panel). Nine-day-old plants were employed for experiments. Arrows highlight differences in primary root length. Bar=1 cm.
B. Measurement of primary root lengths of control and transgenic plants with altered expressions of PLDζ2 grown on medium supplemented with various concentrations of IAA for 9 days confirmed hypersensitivity of PLD2-overexpressing plants to auxin and relative insensitivity of PLD2-deficient plants. Error bars indicate SE (n>40) and statistical analysis was performed using a one-tailed Student’s t test (*, P<0.01).
Li and Xue, Supplemental Figure 2