primer extension, and nuclease mapping

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    PRIMER EXTENSION AND

     NUCLEASE S1 MAPPING.

    BIOCHEMISTRY SEMINAR.ALICE MUMBEY-WAFULA

    FEB, 20TH 2004.

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    PRIMER EXTENSION.

    • Th! ! " #$%h&' (h)h $#*+&! %h$ $#$R$/$!$ T"!)*%"!$,(h)h ! "+!& &( "!

    RNA-'$*$'$% DNA *&+#$"!$.• R$/$!$ T"!)*%"!$ ("! '!)&/$$' 130 H&("' T$# "' D"/' B"+%#&$

    • I% ! &%"$' #"+ 5 %6#&6 )"6!7

    /6!$!, +$ A/" M$+&+"!%&!! 86!.• I% )"%"+!$! %h$ !%h$!! &5 )DNA 6!7 " RNA

    "! " %$#*+"%$.

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    U!$!9

    • P#$ $:%$!& ! 6!$' %& #$"!6$ %h$"#&6% &5 " 7/$ RNA "' %& #"* %h$ ;<

    $' &5 %h"% RNA.• Th$ )$"%& &5 )DNA +"$! 5

    #RNA.

    • D$%$)%& &5 %"!)*%! *&'6)$' '67 /%& %"!)*%&.

    • M"**7 &5 $*+)"%& %"%& *&%.

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    P)*+$ &5 A"+!!

    • L$ "++ &%h$ *&+#$"!$!, %h! $#$ $=6$!

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    • I %h! *&%&)&+, %h$ *#$ $:%$!& ! 6!$' %&#"* %h$ ;

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    • Th$ $!6+%"% )DNA 5&#$' ! ""+$' &

    " !$=6$)7 7$+.• Th$ +$7%h &5 %h$ $:%$'$' *#$ #"*! %h$

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    $+' &5 *#$ $:%$!& *&'6)% $5+$)%!%h$ "6'")$ &5 %h$ RNA.

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    M"%$"+!9

    A$"+7 #:9

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    - R"'&-+"$+$' &+7&6)+$&%'$! >*#$ (%h

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    - 0.;M M7.C+2

    - RN"!$ 5$ ("%$.

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    M"%$"+!9

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    O%h$ #"%$"+!9

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    M$%h&'9

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    Th$ !"#*+$ !

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    P&+")+"#'$ 7$+ $+$)%&*h&$!!, !"#*+$!

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      Th! #&!%+ &))6! G-C )h !%$%)h$!.

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     NUCLEASE S1 MAPPING.

    B")7&6'9

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    • I% ! !&+"%$' 5 A!*$7++6! &"$"#+"!$.

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    %! ")%/$ !%$.

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    • I! %h$#&!%"+$ $!!%"% %&

    '$"%6"%!h7h !"+% )&)$%"%&.

    • I% (&! $!% " ")') PH.

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    U!$!9

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    +"$+$' *&$.

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    h'!.

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    M"%$"+!9

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