probe comparison 052008 - biocat
TRANSCRIPT
Stem and Loop
qPCRCHEMISTRIES
TAQ MAN®MOLECULAR
BEACONS™
AMPLIFLUOR®
PRIMERS
Stem and Loopwith Primer
Linear
STRUCTURE
K EY TRAITS
SPECIFICITY
DESIGN
SOFTWARE
ADVANTAGES
Simplicity of design»Great value with »
powerful multiplexing capabilities
RealTimeDesign™
****
Dual-labeled, linear, »sequence speci�c probe
Used with a pair of »forward and reverse primers
Very low baseline »�uorescence
High level of »speci�city with hairpin structure
Excellent Signal:Noise»
Beacon DesignerPREMIER Biosoft
*****
Dual-labeled hairpin »probe with sequence speci�c loop
Used with a pair of »forward and reverse primers
Very low baseline »�uorescence
Easily adaptable for »di�erent applications
RealTimeDesign™
***
Dual-labeled hairpin »with sequence speci�c primer
One reverse primer»
COMMON
APPLICATIONS
Gene ExpressionIn-Vitro Diagnostics
Allelic Discrimination
Gene ExpressionIn-Vitro Diagnostics
Allelic Discrimination
SNP DetectionGene Expression
Stem and Loopwith Primer
SCORPION™
PRIMERS
Unimolecular»structure incorporates both probe and primer
Fast amplicon »detection
Excellent Signal:Noise »
Beacon DesignerPREMIER Biosoft
*****
Gene ExpressionSNP Detection
In-Vitro DiagnosticsAllelic Discrimination
Dual-labeled hairpin »probe with sequence speci�c loop and primer
One reverse primer»
Labeled Primers with Modi�ed Bases
PLEXOR™
PRIMERS
Simplest to design »(only primers)
Does not require a »separate probe
Powerful multiplexing »capabilities
Promega Plexor PrimerDesign Software
***
One forward primer, »with modi�ed iso-dC and 5’ �uorophore
Modi�ed iso-dG with »quencher in reaction mix
Gene ExpressionSNP Genotyping
BHQPLUS™
PROBES
Linear
Shortened sequences »permit enhanced target speci�city
Discriminate dif�cult »targets such as SNPs and AT-rich regions
RealTimeDesign™
*****
Same key traits as »TaqMan Probes
Compact oligos »forti�ed for binding stabilization
SNP Genotyping
Probe and Primer Comparison Chart
BioCat GmbH Im Neuenheimer Feld 584 D-69120 Heidelberg Tel.: +49 6221 7141516 E-Mail: [email protected] www.biocat.com