procedure for canine major* crossmatch. canine only · 5 clean test tubes (5ml) n°2: xm test...
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5 Clean test tubes (5ml)
N°2: XM TEST PROCEDURE
1 2
4With blood
collector stripIn the clean test tube
1Donor’s tube
ORDonor’s blood bag segment
Bu�er
EDTA
D
Additional material needed:1 centrifuge
5 XM membranes
1 Wash Buffer
1 white top buffer 1
5 Blood collector strips
5 Small pipettes
Material provided:
5 Large pipettes
1 green top buffer 2
1 2
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Canine Crossmatch TestStore at room temperature
Bu�er Bu�er WASH BUFFER
Procedure for canine major* crossmatch. CANINE ONLY
N°1: PREPARATION OF BLOOD SAMPLES
Recipient Donor
Serum or plasma
pRBCs(packed Red Blood Cells)
• EDTA• ACD
Do not use Heparin
• CPD• Plain tube
Centrifuge blood tube in order to collect :
Centrifuge blood tube.Discard the serum or plasma to collect pRBCs.
OR
Collect blood from blood bag segment.
pRBCs
R D
(MAJOR = Donor RBC + Recipient serum or plasma)
* For minor XM reverse the blood samples : Minor = Donor Serum / Plasma + Recipient RBC and perform the same procedure
3 4
5
6 7 8
Up to 5min
Recipient’s tube
Mix gently & incubate 10’ at room temperature
Suspension washing procedure
A B C
3ML
With the large pipette
2
2Bu�er
EDTA
R
Add
Insert the XM membrane.
WASH BUFFER
With the small pipette
3
of serum or plasma in
the test tube
Suspension washing procedure STEP 5 MUST BE DONE 3 TIMES
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Read the result
10min
C
2min
Don’t forget the balance tube
Discard the supernatant
Centrifuge
1000 g
Mix gently
Mix gently
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Incompatible XM + Compatible XM -RESULT
Date :
Name/N° of recipient :
Lab Test lot number :
Name/N° of donor :
Test performed by :
TO INTERPRET YOUR RESULT, STICK THE MEMBRANE BELOW
+ -IncompatibilityDO NOT TRANSFUSE
CompatibilitySAFE TRANSFUSION
XM= CROSSMATCH TEST C = CONTROL LINE
RESULT FORM CANINEfor Crossmatch Test (XM)
Weak line= positive result
INTERPRETATION OF RESULTS
Read immediately after testing
XMC
!In case of any other result, please take a picture and send us an email at : [email protected]
White line= negative result