PRODUCTION OF HIGHLY PURIFIED BOVINE

TRANSFERRIN

Group 5BArjun Goel, Connie Marcelli,

Lianne Kark, Sara Dang, Vivien Tai

Faculty Advisor Prof C.A. Mims

TimetableTask

3 10 17 24 1 8 15 22 29 5 12 19 26Obtain Chemical & Physical Info and Thermodynamic & Kinetic DataAlternatives Available within design briefFirst ReportScalingProject Background

24-Sep

Second Report--Scope of Design; Timetable of Design; Allocation of Jobs within Team

9-OctProcess Flow Diagram NO MASS & ENERGY BALANCES

22-Oct Basis of Design Note

26-Oct

Frozen PFD w/ Mass & Energy Balances; Major Process Streams; Major Process EquipmentSpecification of Process Equipment--Sizing Based on Mass Balance; Auxiliary Equipment Specification; Material Selection; Waste recycle/treatment/disposalKEY UNIT DESIGNP&ID SafetyEnvironmental ImpactControlControl & Operating Philosophy--Normal OperationControl & Operating Philosophy--Startup/Shutdown OperationPlant Layout--General; Safety & Fire; Spacing RecommendationsCost Estimates--Capital Costs; Operating Costs; Selling Price/Unit Price; IRR/NPV analysisEconomic FeasibilityEnergy Efficiency

1-Nov Scheme of Final Report9-Nov Present Preliminary Layout

26-Nov Final Report & DrawingsProcess UtilitiesRecommendationsSocio Economic ImpactEditorExecutive Summary

September October November

Agenda

Mass Balances & Waste Treatment

Key Unit Discussion

Unit Specifications

Current Challenges

Process Overview

Crude Paste

Filtration

Chromatography

Dry Transferrin Powder

Agenda

Mass Balances & Waste Treatment

Key Unit Discussion

Unit Specifications

Current Challenges

Mass Balance — FeedsStarting Paste

18 kg

D.I. Water 571 L

Celite 6 kg

FeCl3, NaHCO3, Na-Citrate for iron Binding

Diafiltration BuffersWash for

Column

EDTA for Iron Chelating

Process

Distilled Water 160 L

Mass Balance — Products

Water 740 L

Celite 6 kg

Inorganics FeCl3, NaHCO3,

Na-Citrate, EDTA, NaOH

Purified Apotransferrin 350g / Batch

Process

Trace Proteins

Ethanol 4.5 kg

Solids Liquids

Immunoglobins for recovery

Waste Treatment--LiquidsOption 1 Option 2

NeutralizeAcid/Base Addition

To Sewer

Waste Water

Solid Waste

Waste Water

Process

Reverse Osmosis

Ion Exchange

Waste Treatment — Solids

1. Reverse Osmosis cartridges

2. Filter press cakes

Disposal via contractor

• Mainly salts and celite trapped in filters

Agenda

Mass Balances & Waste Treatment

Key Unit Discussion

Unit Specifications

Current Challenges

Key Unit: R-101

Wet Paste

2

4

5

R-101

Celite Filter Aid

3

Deionized water

NaOH

P-101

1

Key Unit: R-101

• Stirred Tank• Cylindrical• Conical Bottom • Tank Material: SS316• In Cold Room 0-5 oC

Key Unit: R-101

• Volume of solution in tank: 400 L

• Unit Selected:– Capacity: 757 L ( 200 Gal )– Depth: 1.46 m– Diameter: 0.91m

Key Unit Accessories

• Level Indicator

• pH Monitor

• Temperature Indicator

• SS316 piping

• Valve

• Air Diaphragm Pump

• Mixer

Agenda

Mass Balances &Waste Treatment

Key Unit Discussion

Unit Specifications

Current Challenges

Filter Press: FP-101

• 12” in diameter• Stainless Steel

plates and frames• Cellulose Pads• Four 1” sludge

frames• 40 psi• Cost: $6 703

Filter Press: FP-1013 Applications:

1) 4 Plates: removes 12 000 g paste @ 19 L/min

2) 2 Plates: removes 1 320 g denatured protein @ 9 L/min

3) 2 Plates: collects 3 600 g precipitated protein @ 9 L/min

PrefiltrationSF-101

• Before ultrafiltration

• Millipore low protein binding cartridge filter

• Filtration Area: 0.7m2

• 80 psi

• Entrains particles > 0.5 m

• US$208 for 2

Ultrafiltration

Spiral Wound FilterUF-101

• Concentration: 450 L to 90 L

• Polysulfone

• Flow: 100 L/hr

• Flux: 22 L/m2 hr

• Pressure: 60 PSI

• Cost: $8 212

Spiral Wound FilterUF-102

• Concentrate from 30L to 15L

• Regenerated cellulose Membrane

• Pressure: 60 PSI

• Flow: 10L/hr

• Flux: 11 L/m2hr

• Cost: $2 154

Hollow FiberUF-201

• Concentrate from 12 L to 6 L

• Polysulfone• 25 PSI• 6 L/hr• 30 L/ m2hr• Peristaltic Pump

                                               

        

DEAE Column

1

C-201

To Waste

To Waste5

DEAE Sepharose Fast Flow Column: C-201

• Automated

• Includes: The Column

The Bioprocess Skid

The computer

DEAE Resin

• Total Cost: CND$211, 000

Water Distillation ColumnT-204

• Rate: 190 L/24 hrs• Storage capacity:

280 L• Size:

2m x 1m x 0.6m

• Cost : US$ 3 945

Deionized Water UnitI-101

• Flow range:

15 – 23 L/min• Size:

1.5m x 0.6m x 0.4m• Cost:

US$ 4 142

LyophilizerL-101

• Similar unit used in

plant

• Capacity: 8-12 L• ~6 L of water to

condense per batch

Future Directions

• Key unit development– P&ID– Design equations– Control Philosophy

• Economic Evaluation

• Plant Layout

• Appendices