prof. frank's review powerpoint parasites
TRANSCRIPT
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LAB REVIEW
PARASITES&
BLOOD CELLS
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Band Neutrophil
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Red Blood Cells
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Lymphocyte
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Eosinophil
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Platelets
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Basophil (in horses it is blue)
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Monocyte
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Segmented Neutrophil
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Tapeworm
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Hookworm
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Heartworm Microfilaria
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Coccidia
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Whipworm
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Scabies Mite
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Giardia
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FLEA
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Otodectes Cynotis Mite
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Babesia Canis
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Sucking Lice…………..Biting Lice
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Dermacentor Variabilis….Black Leg Tick
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Lone Star Tick………American Dog Tick
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Dirofilaria immitum….dipeteloma reconditum
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Avian Blood
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Warble or Cuterebra (fly larvae)
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Anaplasma Marginale
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Lung Worm
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paragonimus kellicotti
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Lung Fluke
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Eucoleus boehmi
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Urine
Physical Exam:
Smell
Color
Turbidity
Foam
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Urine
Chemical Exam:pH
Bilirubin
Protein
Occult Blood
Glucose
Ketones
Specific gravity
Nitrites
WBCs
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Heartworm Test
Buffy Coat method (hematocrit test)
Modified Knott’s technique
Filter membrane technique/Di-fil
Blood Smear
SNAP: Antigen testing does not detect the actual parasite but the host’s response to the
disease
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Steps For Modified Knotts
Mix 1 ml of blood w/ 9ml of 2% formalin in centrifuge
Centrifuge tube at 1300 to 1500 rpm for 5 minutes
Pour off liquid supernatant, leaving sediment at bottom of tube
Add 2-3 drops of stain to sediment. Using pipette, mix sediment w/stain
Place drop of this mixture on glass slide. Apply coverslip. Examine for microfilariae using 10X objective.
Filter MembraneMix 1 ml of blood w/9 ml of lysing solution in 15 ml test tube
Place filter membrane in filter holder, close tightly, do not pinch seal in threads of holder
Connect syringe to filter holder and gently empty through filter holder
Fill syringe with water and repeat above step
Open holder, use thumb forceps to remove filter membrane
Place filter membrane on a clean microscope slide
Add one drop of stain, cover with coverslip, examine for microfilariae
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Stages of a Parasites Life Cycle
• 1. oocyst
• 2. Larvae
• 3. nymph
• 4. Pupa
• 5. Adult