professor director of graduate studies in pathology co ... · uropathogenic e. coli, salmonella...

Professor Director of Graduate Studies in Pathology Co-Director of Duke Summer Research Opportunities Program

Soman Abraham, Ph.D. Office Address Edwin L. Jones Research Building 207 Research Drive, Room 257 Durham, NC 27705 Office Phone: 919-684-3630 Email: [email protected] Laboratory Edwin L. Jones Research Building 207 Research Drive, Rooms 251, 253, 255 Durham, NC 27705 Lab Phone: 919-684-6942

Biography ● Publications ● Current Lab Members ● Alumni ● In the News ● Lab Website This laboratory focuses on two main research areas: (a) Elucidating the role of mast cells in modulating immune responses to microbial pathogens. Our studies have revealed that mast cells play a key sentinel role and upon bacterial or viral infection, modulate both innate and adaptive immune responses through the release of immunomodulatory molecules borne in granules. Our current investigations are centered on elucidating the molecular and cellular aspects of how mast cells mediate their immunomodulatory role. We are also examining several mast cell-targeted strategies to boost immunity to infections as well as reduce any pathological consequences of infection. (b) Investigating cross-talk between the immune system and distinct infectious agents such as Uropathogenic E. coli, Salmonella typhimurium and Yersinia pestis. We have recognized that different pathogens possess distinct mechanisms to evade or coopt one or more immune cells to establish infection. Our studies now dwell on developing novel pathogen-specific strategies to combat infection.

Select Publications (Out of 156) 1. Miao Y WJ, Abraham SN. . Ubiquitination of TRAF3 Orchestrates Expulsion of Intracellular Bacteria

by Exocyst Complex. Immunity 2016 (In Press).

2. Miao Y, Li G, Zhang X, Xu H, Abraham SN. A TRP Channel Senses Lysosome Neutralization by

Pathogens to Trigger Their Expulsion. Cell 2015, 161 (6):1306-1319.

3. Abraham SN, Miao Y. The nature of immune responses to urinary tract infections. Nat Rev Immunol

2015, 15 (10):655-663.

4. St John AL, Ang WX, Huang MN, Kunder CA, Chan EW, Gunn MD, Abraham SN. S1P-Dependent

trafficking of intracellular yersinia pestis through lymph nodes establishes Buboes and systemic infection. Immunity 2014, 41 (3):440-450.

5. St John AL, Rathore AP, Raghavan B, Ng ML, Abraham SN. Contributions of mast cells and vasoactive

products, leukotrienes and chymase, to dengue virus-induced vascular leakage. Elife 2013, 2 e00481.

6. Karhausen J, Qing M, Gibson A, Moeser AJ, Griefingholt H, Hale LP, Abraham SN, Mackensen GB.

Intestinal mast cells mediate gut injury and systemic inflammation in a rat model of deep hypothermic circulatory arrest. Crit Care Med 2013, 41 (9):e200-210.

7. Choi HW, Brooking-Dixon R, Neupane S, Lee CJ, Miao EA, Staats HF, Abraham SN. Salmonella

typhimurium impedes innate immunity with a mast-cell-suppressing protein tyrosine phosphatase, SptP. Immunity 2013, 39 (6):1108-1120.

8. Chan CY, St John AL, Abraham SN. Mast cell interleukin-10 drives localized tolerance in chronic

bladder infection. Immunity 2013, 38 (2):349-359.

9. St John AL, Chan CY, Staats HF, Leong KW, Abraham SN. Synthetic mast-cell granules as adjuvants to

promote and polarize immunity in lymph nodes. Nat Mater 2012, 11 (3):250-257.

10. St John AL, Rathore AP, Yap H, Ng ML, Metcalfe DD, Vasudevan SG, Abraham SN. Immune

surveillance by mast cells during dengue infection promotes natural killer (NK) and NKT-cell recruitment and viral clearance. Proc Natl Acad Sci U S A 2011, 108 (22):9190-9195.

11. Abraham SN, St John AL. Mast cell-orchestrated immunity to pathogens. Nat Rev Immunol 2010, 10

(6):440-452.

12. St John AL, Abraham SN. Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines. Nat Med 2009, 15 (11):1259-1265.

13. Song J, Bishop BL, Li G, Grady R, Stapleton A, Abraham SN. TLR4-mediated expulsion of bacteria

from infected bladder epithelial cells. Proc Natl Acad Sci U S A 2009, 106 (35):14966-14971.

14. Shelburne CP, Nakano H, St John AL, Chan C, McLachlan JB, Gunn MD, Staats HF, Abraham SN. Mast

cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues. Cell Host Microbe 2009, 6 (4):331-342.

15. Kunder CA, St John AL, Li G, Leong KW, Berwin B, Staats HF, Abraham SN. Mast cell-derived particles

deliver peripheral signals to remote lymph nodes. J Exp Med 2009, 206 (11):2455-2467.

16. McLachlan JB, Shelburne CP, Hart JP, Pizzo SV, Goyal R, Brooking-Dixon R, Staats HF, Abraham SN.

Mast cell activators: a new class of highly effective vaccine adjuvants. Nat Med 2008, 14 (5):536-541.

17. Song J, Duncan MJ, Li G, Chan C, Grady R, Stapleton A, Abraham SN. A novel TLR4-mediated

signaling pathway leading to IL-6 responses in human bladder epithelial cells. PLoS Pathog 2007, 3 (4):e60.

18. Song J, Bishop BL, Li G, Duncan MJ, Abraham SN. TLR4-initiated and cAMP-mediated abrogation of

bacterial invasion of the bladder. Cell Host Microbe 2007, 1 (4):287-298.

19. Bishop BL, Duncan MJ, Song J, Li G, Zaas D, Abraham SN. Cyclic AMP-regulated exocytosis of

Escherichia coli from infected bladder epithelial cells. Nat Med 2007, 13 (5):625-630.

20. McLachlan JB, Hart JP, Pizzo SV, Shelburne CP, Staats HF, Gunn MD, Abraham SN. Mast cell-derived

tumor necrosis factor induces hypertrophy of draining lymph nodes during infection. Nat Immunol 2003, 4 (12):1199-1205.

21. Shin JS, Gao Z, Abraham SN. Involvement of cellular caveolae in bacterial entry into mast cells.

Science 2000, 289 (5480):785-788.

22. Malaviya R, Gao Z, Thankavel K, van der Merwe PA, Abraham SN. The mast cell tumor necrosis

factor alpha response to FimH-expressing Escherichia coli is mediated by the glycosylphosphatidylinositol-anchored molecule CD48. Proc Natl Acad Sci U S A 1999, 96 (14):8110-8115.

23. Thankavel K, Madison B, Ikeda T, Malaviya R, Shah AH, Arumugam PM, Abraham SN. Localization of

a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor

recognition and use of a domain-specific antibody to confer protection against experimental urinary tract infection. J Clin Invest 1997, 100 (5):1123-1136.

24. Baorto DM, Gao Z, Malaviya R, Dustin ML, van der Merwe A, Lublin DM, Abraham SN. Survival of

FimH-expressing enterobacteria in macrophages relies on glycolipid traffic. Nature 1997, 389 (6651):636-639.

25. Malaviya R, Ikeda T, Ross E, Abraham SN. Mast cell modulation of neutrophil influx and bacterial

clearance at sites of infection through TNF-alpha. Nature 1996, 381 (6577):77-80.

Alumni & publications while in the Abraham Lab

Yuxuan (Phoenix) Miao, BS, PhD (Duke Molecular Genetics & Microbiology Graduate program) Jane Coffin Childs Postdoctoral Fellow at Rockefeller University, New York City, NY, Laboratory of Mammalian Cell Biology and Development. Publications from the Abraham lab. Miao, Y., Wu J & Abraham, S. N (2016). Ubiquitination of Innate Immune Regulator TRAF3 Orchestrates Expulsion of Intracellular Bacteria by Exocyst Complex. Immunity (In Press) Abraham, S. N., & Miao, Y. (2015). The nature of immune responses to urinary tract infections. Nature Reviews Immunology, 15(10), 655-663. Miao, Y., Li, G., Zhang, X., Xu, H., & Abraham, S. N. (2015). A TRP channel senses lysosome neutralization by pathogens to trigger their expulsion. Cell, 161(6), 1306-1319. Miao, Y., & Abraham, S. N. (2014). Kidney α–intercalated cells and lipocalin 2: defending the urinary tract. The Journal of clinical investigation, 124(7), 2844-2846. Miao, Y., & Abraham, S. N. (2014). Peeing Pentraxins. Immunity, 40(4), 460-462.

Ashley St. John, PhD (Duke Immunology Graduate program) Assistant Professor, Program in Emerging Infectious Diseases, Duke-NUS Email: [email protected] Interests: Lymph node remodeling during infection, viral immunology, vaccination. Publications from the Abraham lab. John, A. L. S., Ang, W. G., Huang, M. N., Kunder, C. A., Chan, E. W., Gunn, M. D., & Abraham, S. N. (2014). S1P-Dependent trafficking of intracellular yersinia pestis through lymph nodes establishes Buboes and systemic infection. Immunity, 41(3), 440-450. John, A. L. S., Abraham, S. N., & Gubler, D. J. (2013). Barriers to preclinical investigations of anti-dengue immunity and dengue pathogenesis. Nature Reviews Microbiology, 11(6), 420-426. St John, A. L., Rathore, A. P., Raghavan, B., Ng, M. L., & Abraham, S. N. (2013). Contributions of mast cells and vasoactive products, leukotrienes and chymase, to dengue virus-induced vascular leakage. Elife, 2, e00481. John, A. L. S., & Abraham, S. N. (2013). Innate immunity and its regulation by mast cells. The Journal of Immunology, 190(9), 4458-4463. Chan, C. Y., John, A. L. S., & Abraham, S. N. (2013). Mast cell interleukin-10 drives localized tolerance in chronic bladder infection. Immunity, 38(2), 349-359.

mailto:[email protected]

John, A. L. S., Chan, C. Y., Staats, H. F., Leong, K. W., & Abraham, S. N. (2012). Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes. Nature materials, 11(3), 250-257. Chan, C. Y., John, A. L. S., & Abraham, S. N. (2012). Plasticity in mast cell responses during bacterial infections. Current opinion in microbiology, 15(1), 78-84. Kunder, C. A., St John, A. L., & Abraham, S. N. (2011). Mast cell modulation of the vascular and lymphatic endothelium. Blood, 118(20), 5383-5393. John, A. L. S., Rathore, A. P., Yap, H., Ng, M. L., Metcalfe, D. D., Vasudevan, S. G., & Abraham, S. N. (2011). Immune surveillance by mast cells during dengue infection promotes natural killer (NK) and NKT-cell recruitment and viral clearance. Proceedings of the National Academy of Sciences, 108(22), 9190-9195. Abraham, S. N., & John, A. L. S. (2010). Mast cell-orchestrated immunity to pathogens. Nature Reviews Immunology, 10(6), 440-452. St John, A. L., & Abraham, S. N. (2009). Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines. Nature medicine, 15(11), 1259-1265. Shelburne, C. P., Nakano, H., John, A. L. S., Chan, C., McLachlan, J. B., Gunn, M. D., ... & Abraham, S. N. (2009). Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues. Cell host & microbe, 6(4), 331-342. Kunder, C. A., John, A. L. S., Li, G., Leong, K. W., Berwin, B., Staats, H. F., & Abraham, S. N. (2009). Mast cell–derived particles deliver peripheral signals to remote lymph nodes. The Journal of experimental medicine,206(11), 2455-2467.

Cheryl Chan, PhD (Duke Pathology Graduate program) Post-doctoral fellow, Duke-NUS Email: [email protected] Interests: Mast cell modulation of immunity in the bladder. Publications from the Abraham lab. John, A. L. S., & Abraham, S. N. (2013). Innate immunity and its regulation by mast cells. The Journal of Immunology, 190(9), 4458-4463. Chan, C. Y., John, A. L. S., & Abraham, S. N. (2013). Mast cell interleukin-10 drives localized tolerance in chronic bladder infection. Immunity, 38(2), 349-359. John, A. L. S., Chan, C. Y., Staats, H. F., Leong, K. W., & Abraham, S. N. (2012). Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes. Nature materials, 11(3), 250-257. Chan, C. Y., John, A. L. S., & Abraham, S. N. (2012). Plasticity in mast cell responses during bacterial infections. Current opinion in microbiology, 15(1), 78-84. Shelburne, C. P., Nakano, H., John, A. L. S., Chan, C., McLachlan, J. B., Gunn, M. D., ... & Abraham, S. N. (2009). Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected tissues. Cell host & microbe, 6(4), 331-342. Terada, N., Ohno, N., Saitoh, S., Saitoh, Y., Fujii, Y., Kondo, T., ... & Ohno, S. (2009). Involvement of dynamin-2 in formation of discoid vesicles in urinary bladder umbrella cells. Cell and tissue research, 337(1), 91-102.

mailto:[email protected]

Song, J., Duncan, M. J., Li, G., Chan, C., Grady, R., Stapleton, A., & Abraham, S. N. (2007). A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. PLoS Pathog, 3(4), e60.

Samantha Bowen, BS, PhD (Duke Molecular Genetics & Microbiology Graduate program) Clinical Trials Project Leader I at Duke Clinical Research Institute, Duke University Medical Center, Durham, NC Publications from the Abraham lab. Bowen, S. E., Watt, C. L., Murawski, I. J., Gupta, I. R., & Abraham, S. N. (2013). Interplay between vesicoureteric reflux and kidney infection in the development of reflux nephropathy in mice. Disease Models and Mechanisms, 6(4), 934-941.

Chen, Y. L., Konieczka, J. H., Springer, D. J., Bowen, S. E., Zhang, J., Silao, F. G. S., ... & Thompson, D. A. (2012). Convergent evolution of calcineurin pathway roles in thermotolerance and virulence in Candida glabrata. G3: Genes| Genomes| Genetics, 2(6), 675-691.

Yuvon Mobley, BS, PhD (Duke Molecular Genetics & Microbiology Graduate program) Medical Science Liaison at Genesight, Mason, Ohio

Ravi Malaviya, PhD

(Postdoctoral fellow, 1992-1996)

Current Position: Senior Scientist at Johnson & Johnson, New Jersey

Publications from the Abraham lab. Contribution of mast cells to bacterial clearance and their proliferation during experimental cystitis

induced by type 1 fimbriated E. coli.Malaviya R, Ikeda T, Abraham SN, Malaviya R.Immunol Lett.

2004 Feb 15;91(2-3):103-11.

Mast cell modulation of immune responses to bacteria.Malaviya R, Abraham SN.Immunol

Rev. 2001 Feb;179:16-24.

Type 1 fimbriated Escherichia coli-mast cell interactions in cystitis.Abraham S, Shin J, Malaviya R.J

Infect Dis. 2001 Mar 1;183 Suppl 1:S51-5.

Mast cell modulation of the innate immune response to enterobacterial infection.Abraham SN, Malaviya

R.Adv Exp Med Biol. 2000;479:91-105.

Role of mast cell leukotrienes in neutrophil recruitment and bacterial clearance in

infectious peritonitis.Malaviya R, Abraham SN.J Leukoc Biol. 2000 Jun;67(6):841-

6.

The mast cell tumor necrosis factor alpha response to FimH-expressing Escherichia coli is mediated

by the glycosylphosphatidylinositol-anchored molecule CD48.Malaviya R, Gao Z, Thankavel K, van

der Merwe PA, Abraham SN.Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8110-5.

Clinical implications of mast cell-bacteria interaction.Malaviya R, Abraham SN.J Mol

Med. 1998 Aug;76(9):617-23.

Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic.Baorto DM, Gao

Z, Malaviya R, Dustin ML, van der Merwe A, Lublin DM, Abraham SN.Nature. 1997 Oct

9;389(6651):636-9.

Mast cells in infection and immunity.Abraham SN, Malaviya R.Infect Immun. 1997 Sep;65(9):3501-8.

Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of

receptor recognition and use of a domain-specific antibody to confer protection against

experimental urinary tract infection.Thankavel K, Madison B, Ikeda T, Malaviya R, Shah AH,

Arumugam PM, Abraham SN.J Clin Invest. 1997 Sep 1;100(5):1123-36.

Mast cells as modulators of host defense in the lung.Abraham SN, Thankavel K, Malaviya R.Front

http://www.ncbi.nlm.nih.gov/pubmed/15019277?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum

















































































































































Biosci. 1997 Feb 15;2:d78-87.

Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through

TNF- alpha.Malaviya R, Ikeda T, Ross E, Abraham SN.Nature. 1996 May 2;381(6577):77-

80.

Mast cells process bacterial Ags through a phagocytic route for class I MHC presentation to T

cells.Malaviya R, Twesten NJ, Ross EA, Abraham SN, Pfeifer JD.J Immunol. 1996 Feb

15;156(4):1490-6.


































Bacteria--Mast Cell Interactions in Inflammatory Disease.Malaviya R, Ikeda T, Ross EA, Jakschik

BA, Abraham SN.Am J Ther. 1995 Oct;2(10):787-792.

Interaction of bacteria with mast cells.Malaviya R, Abraham SN.Methods Enzymol.

1995;253:27-43.

The PapG tip adhesin of P fimbriae protects Escherichia coli from neutrophil bactericidal

activity.Tewari R, Ikeda T, Malaviya R, MacGregor JI, Little JR, Hultgren SJ, Abraham SN.Infect

Immun. 1994 Dec;62(12):5296-304.

Mast cell degranulation induced by type 1 fimbriated Escherichia coli in mice.Malaviya R, Ross E,

Jakschik BA, Abraham SN.J Clin Invest. 1994 Apr;93(4):1645-53.

Mast cell phagocytosis of FimH-expressing enterobacteria.Malaviya R, Ross EA, MacGregor JI,

Ikeda T, Little JR, Jakschik BA, Abraham SN.J Immunol. 1994 Feb 15;152(4):1907-14.













































Jeoung Sook Shin

(Duke Pathology Graduate Program, 1997-2002)

Post-doctoral training: Department of Cell Biology, Yale University

Current Position: Associate Professor, Department of Microbiology and Immunology, University of California, San Francisco

Publications from the Abraham lab. Harboring of particulate allergens within secretory compartments by mast cells following

IgE/FcepsilonRI- lipid raft-mediated phagocytosis.Shin JS, Shelburne CP, Jin C, LeFurgey EA,

Abraham SN. J Immunol.

2006 Nov 1;177(9):5791-800.

Bacterial penetration of bladder epithelium through lipid rafts.Duncan MJ, Li G, Shin JS, Carson JL, Abraham SN.J Biol Chem. 2004 Apr 30;279(18):18944-51. Epub 2004 Feb 19.

Microbial entry through caveolae: variations on a theme.Duncan MJ, Shin JS, Abraham SN.Cell Microbiol. 2002 Dec;4(12):783-91.

Cell biology. Caveolae--not just craters in the cellular landscape.Shin JS, Abraham SN.Science. 2001 Aug 24;293(5534):1447-8.

Caveolae as portals of entry for microbes.Shin JS, Abraham SN.Microbes Infect. 2001 Jul;3(9):755-

61.

Glycosylphosphatidylinositol-anchored receptor-mediated bacterial endocytosis.Shin JS,

Abraham SN.FEMS Microbiol Lett. 2001 Apr 13;197(2):131-8.

Type 1 fimbriated Escherichia coli-mast cell interactions in cystitis.Abraham S, Shin J, Malaviya R.J

Infect Dis. 2001 Mar 1;183 Suppl 1:S51-5.

Co-option of endocytic functions of cellular caveolae by pathogens.Shin JS, Abraham

SN.Immunology. 2001 Jan;102(1):2-7.

Involvement of cellular caveolae in bacterial entry into mast cells.Shin JS, Gao Z, Abraham

SN.Science. 2000 Aug 4;289(5480):785-8.
















































































Bacteria-host cell interaction mediated by cellular cholesterol/glycolipid-enriched

microdomains.Shin JS, Gao Z, Abraham SN.Biosci Rep. 1999 Oct;19(5):421-32.

James McLachlan (Duke Pathology Graduate Program, 1998-2003) Current position: Associate Professor, Department of Immunology, University of Tulane

Publications from the Abraham lab.

Mast cells augment adaptive immunity by orchestrating dendritic cell trafficking through infected

tissues. Shelburne CP, Nakano H, St John AL, Chan C, McLachlan JB, Gunn MD, Staats HF, Abraham

SN. Cell Host Microbe. 2009 Oct 22;6(4):331-42. doi: 10.1016/j.chom.2009.09.004.

Mast cell activators: a new class of highly effective vaccine adjuvants.McLachlan JB, Shelburne

CP, Hart JP, Pizzo SV, Goyal R, Brooking-Dixon R, Staats HF, Abraham SN. Nat Med. 2008

May;14(5):536-41. Epub 2008 Apr 20.

In vivo models for studying mast cell-dependent responses to bacterial infection.Shelburne

CP, McLachlan JB, Abraham SN. Methods Mol Biol. 2006;315:363-81.

Mast cell-derived tumor necrosis factor induces hypertrophy of draining lymph nodes

during infection.McLachlan JB, Hart JP, Pizzo SV, Shelburne CP, Staats HF, Gunn

MD, Abraham SN. Nat Immunol. 2003 Dec;4(12):1199-205. Epub 2003 Nov 2.

Studies of the multifaceted mast cell response to bacteria.McLachlan JB, Abraham

SN. Curr Opin Microbiol. 2001 Jun;4(3):260-6.









http://www.ncbi.nlm.nih.gov/pubmed/19837373



































Mathew Duncan (Duke Molecular Genetics & Microbiology Graduate Program, 1998-2004) Current position: CEO at Environmental Conditioning Systems

Publications from the Abraham Lab.

TLR4-initiated and cAMP-mediated abrogation of bacterial invasion of the bladder.Song J, Bishop BL,

Li G, Duncan MJ, Abraham SN.Cell Host Microbe. 2007 Jun 14;1(4):287-98.

A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial

cells.Song J, Duncan MJ, Li G, Chan C, Grady R, Stapleton A, Abraham SN.PLoS Pathog. 2007

Apr;3(4):e60.

Cyclic AMP-regulated exocytosis of Escherichia coli from infected bladder epithelial

cells.Bishop BL, Duncan MJ, Song J, Li G, Zaas D, Abraham SN.Nat Med. 2007

May;13(5):625-30. Epub 2007 Apr 8.

The role of lipid rafts in the pathogenesis of bacterial infections.Zaas DW, Duncan M, Rae

Wright J, Abraham SN.Biochim Biophys Acta. 2005 Dec 30;1746(3):305-13. Epub 2005 Oct

26.

Bacterial penetration of the mucosal barrier by targeting lipid rafts.Abraham SN, Duncan MJ, Li G,

Zaas D.J Investig Med. 2005 Sep;53(6):318-21.

The distinct binding specificities exhibited by enterobacterial type 1 fimbriae are determined by their

fimbrial shafts.Duncan MJ, Mann EL, Cohen MS, Ofek I, Sharon N, Abraham SN.J Biol Chem.

2005 Nov 11;280(45):37707-16. Epub 2005 Aug 23.

Pseudomonas invasion of type I pneumocytes is dependent on the expression and phosphorylation of

caveolin-2.Zaas DW, Duncan MJ, Li G, Wright JR, Abraham SN.J Biol Chem. 2005 Feb

11;280(6):4864-

72. Epub 2004 Nov 15.
























































































Bacterial penetration of bladder epithelium through lipid rafts.Duncan MJ, Li G, Shin JS, Carson JL,

Abraham SN.J Biol Chem. 2004 Apr 30;279(18):18944-51. Epub 2004 Feb 19.

Microbial entry through caveolae: variations on a theme.Duncan MJ, Shin JS, Abraham SN.Cell

Microbiol. 2002 Dec;4(12):783-91.

David Zaas, MD

(Duke University Department of Medicine Pulmonary Fellow, 2003-2007)

Current Position: President, Duke Raleigh Hospital and Vice Chair, Department of Medicine Duke

University, Durham.


Counteracting signaling activities in lipid rafts associated with the invasion of lung epithelial

cells by Pseudomonas aeruginosa.Zaas DW, Swan ZD, Brown BJ, Li G, Randell SH, Degan S,

Sunday ME, Wright JR, Abraham SN. J Biol Chem. 2009 Feb 11. [Epub ahead of print]



May;13(5):625-30. Epub 2007 Apr 8.

Bacterial penetration of the mucosal barrier by targeting lipid rafts.Abraham SN, Duncan MJ, Li G,

Zaas D.J Investig Med. 2005 Sep;53(6):318-21.

Pseudomonas invasion of type I pneumocytes is dependent on the expression and

phosphorylation of caveolin-2.Zaas DW, Duncan MJ, Li G, Wright JR, Abraham SN.J Biol

Chem. 2005 Feb 11;280(6):4864-

72. Epub 2004 Nov 15.

The role of lipid rafts in the pathogenesis of bacterial infections.Zaas DW, Duncan M,

Rae Wright J, Abraham SN. Biochim Biophys Acta. 2005 Dec 30;1746(3):305-13. Epub

2005 Oct 26.

















































































Brian Bishop (Duke Molecular Genetics & Microbiology Graduate program, 2001-2007) Current position: Manager Regulatory Affairs, Emergent BioSolutions


Song J, Bishop BL, Li G, Grady R, Stapleton A, Abraham SN.TLR4-mediated expulsion of bacteria

from infected bladder epithelial cells. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14966-71. doi:

10.1073/pnas.0900527106. Epub 2009 Aug 17.

TLR4-initiated and cAMP-mediated abrogation of bacterial invasion of the bladder.Song J,

Bishop BL, Li G, Duncan MJ, Abraham SN. Cell Host Microbe. 2007 Jun 14;1(4):287-98.



May;13(5):625-30. Epub 2007 Apr 8.

Abraham SN, Bishop, B, Sharon N, Ofek I. Adhesion of Bacteria to Mucosal Surfaces.

In:Mucosal Immunology, J. Mestecky et al. (eds) Academic Press Ltd. CA . 2005; 3:35-

48.

























Rhea Brooking-Dixon (Duke Pathology Graduate Program, 2002-2008) Current Position: Senior Consultant at Booz Allen Hamilton, Strategy and Technology Consulting Firm


Mast cell activators: a new class of highly effective vaccine adjuvants.McLachlan JB, Shelburne CP,

Hart JP,

Pizzo SV, Goyal R, Brooking-Dixon R, Staats HF, Abraham SN. Nat Med. 2008 May;14(5):536-

41. Epub 2008 Apr 20.

Disruption of a nonribosomal peptide synthetase in Aspergillus fumigatus eliminates gliotoxin

production.Cramer RA Jr, Gamcsik MP, Brooking RM, Najvar LK, Kirkpatrick WR, Patterson TF,

Balibar CJ, Graybill JR, Perfect JR, Abraham SN, Steinbach WJ. Eukaryot Cell. 2006 (6):972-80.












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Jeongmin Song (Duke Molecular Genetics & Microbiology Graduate Program, 2003-2008) Current Position: Assistant Professor, Department of Microbiology, Cornell University, Ithaca, NY


Innate and adaptive immune responses in the urinary tract.Song J, Abraham SN.Eur J Clin

Invest. 2008 Oct;38 Suppl 2:21-8.

Song J, Bishop BL, Li G, Grady R, Stapleton A, Abraham SN.TLR4-mediated expulsion of bacteria

from infected bladder epithelial cells. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14966-71. doi:

10.1073/pnas.0900527106. Epub 2009 Aug 17.

TLR-mediated immune responses in the urinary tract.Song J, Abraham SN.Curr Opin

Microbiol. 2008 Feb;11(1):66-73. Epub 2008 Feb 1.

TLR4-initiated and cAMP-mediated abrogation of bacterial invasion of the bladder.Song J,

Bishop BL, Li G, Duncan MJ, Abraham SN.Cell Host Microbe. 2007 Jun 14;1(4):287-98.

A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder

epithelial cells.Song J, Duncan MJ, Li G, Chan C, Grady R, Stapleton A, Abraham SN.PLoS

Pathog. 2007 Apr;3(4):e60.



May;13(5):625-30. Epub 2007 Apr 8.























































Alison Hofmann, MD (Pediatric-Immunology Fellow, Columbia, 2007-2010) Areas of Interests: Immunotherapeutic modulation of mast cells

Current Position: Senior Medical Officer at Glaxo Smith Kline, Research Triangle Park, North Carolina


Hofmann AM, Abraham SN.New roles for mast cells in pathogen defense and allergic disease.Discov

Med. 2010 Feb;9(45):79-83.

Hofmann AM, Abraham SN. New roles for mast cells in modulating allergic reactions and

immunity against pathogens. Curr Opin Immunol. 2009 Dec;21(6):679-86. doi:

10.1016/j.coi.2009.09.007. Epub 2009 Oct 12.





Cong Jin, BS, MS (Peking University) (Cell & Molecular Biology/Molecular Genetics & Microbiology Graduate Program, 2005-

2010)

Areas of Interest: Mast cell interactions with allergens during asthma

Current Position: Associate Professor at Center for Disease Control, Beijing, China

Publications from the Abraham lab Particulate allergens potentiate allergic asthma in mice through sustained IgE-mediated mast

cell activation. Jin C, Shelburne CP, Li G, Potts EN, Riebe KJ, Sempowski GD, Foster WM,

Abraham SN. J Clin Invest. 2011 Mar;121(3):941-55. doi: 10.1172/JCI43584.

Harboring of particulate allergens within secretory compartments by mast cells following

IgE/FcepsilonRI-lipid raft- mediated phagocytosis. Shin JS, Shelburne CP, Jin C, LeFurgey EA,

Abraham SN. J Immunol. 2006 Nov 1;177(9):5791- 800.






Christian Kunder BS (University of Pennsylvania) MD/PhD Cell & Molecular Biology/Pathology Graduate Program, 2004-2009

Areas of Interest: Mast Cell regulation of Immune cell trafficking

Current Position: Assistant Professor, Department of Pathology, Stanford University

Publications from the Abraham lab Mast cell modulation of the vascular and lymphatic endothelium. Kunder CA, St John AL, Abraham SN.

Blood. 2011 Nov 17;118(20):5383-93. doi: 10.1182/blood-2011-07-358432. Epub 2011

Sep 8.

Mast cell-derived particles deliver peripheral signals to remote lymph nodes. Kunder CA, St John AL,

Li G, Leong KW, Berwin B, Staats HF, Abraham SN. J Exp Med. 2009 Oct 26;206(11):2455-67. doi:

10.1084/jem.20090805. Epub 2009 Oct 5.



Guojie Li, PhD (University of Hawaii)

Areas of research Interest: Molecular Biology and Proteomics

Senior Research Scientist at Duke University, Dept. of Pathology

(2002- 2011)

Publications from the Abraham Lab

Particulate allergens potentiate allergic asthma in mice through sustained IgE-mediated mast cell

activation. Jin C, Shelburne CP, Li G, Potts EN, Riebe KJ, Sempowski GD, Foster WM, Abraham

SN. J Clin Invest. 2011 Mar;121(3):941-55. doi: 10.1172/JCI43584.

Mast cell-derived particles deliver peripheral signals to remote lymph nodes. Kunder CA, St John

AL, Li G, Leong KW, Berwin B, Staats HF, Abraham SN. J Exp Med. 2009 Oct 26;206(11):2455-

67. doi: 10.1084/jem.20090805. Epub 2009 Oct 5.

TLR4-mediated expulsion of bacteria from infected bladder epithelial cells. Song J, Bishop BL, Li G,

Grady R, Stapleton A, Abraham SN. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14966-71. doi:

10.1073/pnas.0900527106. Epub 2009 Aug 17.

Counteracting signaling activities in lipid rafts associated with the invasion of lung epithelial cells by

Pseudomonas aeruginosa. Zaas DW, Swan ZD, Brown BJ, Li G, Randell SH, Degan S, Sunday ME,

Wright JR, Abraham SN. J Biol Chem. 2009 Apr 10;284(15):9955-64. doi: 10.1074/jbc.M808629200.

Epub 2009 Feb 11.

TLR4-initiated and cAMP-mediated abrogation of bacterial invasion of the bladder. Song J, Bishop

BL, Li G, Duncan MJ, Abraham SN. Cell Host Microbe. 2007 Jun 14;1(4):287-98.

A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells.

Song J, Duncan MJ, Li G, Chan C, Grady R, Stapleton A, Abraham SN. PLoS Pathog. 2007










Apr;3(4):e60.

Cyclic AMP-regulated exocytosis of Escherichia coli from infected bladder epithelial cells. Bishop

BL, Duncan MJ, Song J, Li G, Zaas D, Abraham SN. Nat Med. 2007 May;13(5):625-30. Epub 2007

Apr 8.

Bacterial penetration of the mucosal barrier by targeting lipid rafts. Abraham SN, Duncan MJ, Li G,

Zaas D. J Investig Med. 2005 Sep;53(6):318-21.

Pseudomonas invasion of type I pneumocytes is dependent on the expression and phosphorylation of

caveolin-2. Zaas DW, Duncan MJ, Li G, Wright JR, Abraham SN. J Biol Chem. 2005 Feb

11;280(6):4864-72. Epub 2004 Nov 15.

Bacterial penetration of bladder epithelium through lipid rafts. Duncan MJ, Li G, Shin JS, Carson JL,

Abraham SN. J Biol Chem. 2004 Apr 30;279(18):18944-51. Epub 2004 Feb 19.






Adam Moeser PhD DVM Current Position: Matilda R. Wilson Endowed Chair and Associate Professor at Large Animal Clinical

Services, College of Veterinary Medicine, Michigan State University Publications from the Abraham lab. Karhausen, J., Qing, M., Gibson, A., Moeser, A. J., Griefingholt, H., Hale, L. P., ... & Mackensen, G. B. (2013). Intestinal mast cells mediate gut injury and systemic inflammation in a rat model of deep hypothermic circulatory arrest. Critical care medicine, 41(9), e200-e210.

Abraham Lab 2008

Abraham lab 2004

Soman Abraham Lab in the News New study of the molecular roots of recurrent bladder infections could lead to a vaccine

Medical Press

http://medicalxpress.com/news/2013-02-molecular-roots-recurrent-bladder-infections.html

Salmonella Disables Bacteria-Fighting Mast Cells During Invasion

Asian Scientist

http://www.asianscientist.com/2013/12/in-the-lab/salmonella-disables-bacteria-fighting-mast-cells-

invasion-2013/

Herb May Cure Bladder Infections

CBS News

http://www.cbsnews.com/news/herb-may-cure-bladder-infections/

Picture will not copy

Understanding How Lymph Nodes Respond To Infection May Redefine How Immune System

Functions

Science Daily

https://www.sciencedaily.com/releases/2003/11/031121072013.htm

No Picture

Nanoparticle trick 'boosts body's vaccine response'

BBC News

http://www.bbc.com/news/health-16654182

http://medicalxpress.com/news/2013-02-molecular-roots-recurrent-bladder-infections.html

http://www.asianscientist.com/2013/12/in-the-lab/salmonella-disables-bacteria-fighting-mast-cells-invasion-2013/

http://www.asianscientist.com/2013/12/in-the-lab/salmonella-disables-bacteria-fighting-mast-cells-invasion-2013/

http://www.cbsnews.com/news/herb-may-cure-bladder-infections/


How the plague became a deadly force

ABC

http://www.abc.net.au/radionational/programs/healthreport/how-the-black-plague-became-a-deadly-

force/6768776

Our Cells Can Vomit In Disgust Too: Bladder Cells Spew Out Nasty E.Coli Germs Responsible For

Uninary Tract Infections As A Defense Mechanism

Medical Daily

http://www.medicaldaily.com/our-cells-can-vomit-disgust-too-bladder-cells-spew-out-nasty-ecoli-germs-

responsible-335556

Salmonella jams signals from bacteria-fighting mast cells

Science Daily


http://www.abc.net.au/radionational/programs/healthreport/how-the-black-plague-became-a-deadly-force/6768776

http://www.abc.net.au/radionational/programs/healthreport/how-the-black-plague-became-a-deadly-force/6768776

http://www.medicaldaily.com/our-cells-can-vomit-disgust-too-bladder-cells-spew-out-nasty-ecoli-germs-responsible-335556

http://www.medicaldaily.com/our-cells-can-vomit-disgust-too-bladder-cells-spew-out-nasty-ecoli-germs-responsible-335556


Researchers pursuing powdered nasal vaccine against West Nile

Medical press

http://medicalxpress.com/news/2014-05-pursuing-powdered-nasal-vaccine-west.html

New cell that attacks dengue virus identified

Science Daily


http://medicalxpress.com/news/2014-05-pursuing-powdered-nasal-vaccine-west.html


professor director of graduate studies in pathology co ... · uropathogenic e. coli, salmonella...

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