program will begin shortly… “inner life of the cell”, harvard university © 2008 up next …...
TRANSCRIPT
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Program will begin shortly… “Inner Life of the Cell”, Harvard University © 2008
Up Next …
Making Molecular Movies with Up Next …
Making Molecular Movies with
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Making Molecular Movies with
Making Molecular Movies with
Your Name, Institution
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The Team The Team
Peter KnerPeter KnerMicroscopistMicroscopist
Jessica WinterJessica Winter Chemical EngineerChemical Engineer
Ge YangGe Yang BioengineerBioengineer
Beth BrainerdBeth BrainerdBiologistBiologist
University of Georgia
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qstorm.orgVisit us online!
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qstorm.orgVisit us online!
Time is ticking. Can these folks make a major breakthrough in
biological imaging?
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48 hours in 40 seconds
Credit: www.broad.com; The Ark Hotel
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10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
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10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
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10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
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10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
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10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
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The Team The Team
Peter KnerPeter KnerMicroscopistMicroscopist
Jessica WinterJessica Winter Chemical EngineerChemical Engineer
Ge YangGe Yang BioengineerBioengineer
Beth BrainerdBeth BrainerdBiologistBiologist
University of Georgia
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Beth BrainerdBiologist
(Muscle Cells)
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Zebrafish Muscle Cells
Credit: Mezzotint
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Ge YangBioengineer (Nerve Cells)
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Credit: “Inside the Brain”, NIH National Institute on Aging
Nerve Cells
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“Inner Life of the Cell”, Harvard University © 2008
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“Inner Life of the Cell”, Harvard University © 2008
≈ 100 nanometers
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“Inner Life of the Cell”, Harvard University © 2008
≈ 100 nanometers
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Motor Protein
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Much smaller than a wavelength of visible light
30 nm
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Point light source
30 nm
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Optical Limit: 200 nanometers
200 nm
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Overlapping Blurs
200 nm
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The Team The Team
Peter KnerPeter KnerMicroscopistMicroscopist
Jessica WinterJessica Winter Chemical EngineerChemical Engineer
Ge YangGe Yang BioengineerBioengineer
Beth BrainerdBeth BrainerdBiologistBiologist
University of Georgia
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Peter KnerMicroscopist
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STORM
Credit: MovingImages
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STORMIMAGING
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STORM
STochastic
Optical
Reconstruction
Microscopy
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STORM
Xiaowei Zhuang 2006
Credit: FredField.com
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Standard optical microscope image
Credit: Bates et al. Science 317, 1749-1753 (2007)
3 μm
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Loss of resolution when zoomed into the nanoscale
Credit: Bates et al. Science 317, 1749-1753 (2007)
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Credit: Bates et al. Science 317, 1749-1753 (2007)
Standard Microscope Image Diffraction-Limited
STORM ImageSuper Resolution
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Localization
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Greetings from
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Credit: Ricardo Henriques, Institut Pasteur
STORM Imaging at Work
Stochastic (Random)Blinking
Reconstruction
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STORM Imaging at Work
Particle Detectionand Localization
Reconstruction
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STORM Imaging of Cell Structures
Credit: Rust et al. Nature Methods 3, 793-795 (2006) and Bates et al. Science 317, 1749-1753 (2007)
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Credit: Bates et al. Science 317, 1749-1753 (2007)
STORM – Depends on bright light sources
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Credit: Bates et al. Science 317, 1749-1753 (2007)
Less than 30 nm !
STORM – Depends on bright light sources
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Credit: Bates et al. Science 317, 1749-1753 (2007)
STORM – How to Get Even Greater Resolution?
Less than 30 nm !
We need more photons!
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The Team The Team
Peter KnerPeter KnerMicroscopistMicroscopist
Jessica WinterJessica Winter Chemical EngineerChemical Engineer
Ge YangGe Yang BioengineerBioengineer
Beth BrainerdBeth BrainerdBiologistBiologist
University of Georgia
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Jessica WinterChemical Engineer
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Quantum Dots in Solution
Credit: Felice Frankel
Nano Crystals of Different Sizes
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Jessica WinterChemical Engineer
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Credit: Ricardo Henriques
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10 nm
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Linker
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SuperHeroes
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qstorm.orgVisit us online!
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An MOS Strategic Projects Productionby C.L. Alpert and K. Thatewith assistance from J. Antilland the QSTORM Research Collaborative
Production of this project was supported by the National Science Foundation, MCB-1052733. Dissemination of this project was supported by the NISE Network through the National Science Foundation under Award No. 0940143.Any opinions, findings, and conclusions or recommendations are those of the author(s) and do not necessarily reflect the views of the Foundation.
Published under a Creative Commons Attribution-Noncommercial-ShareAlike license: http://creativecommons.org/licenses/by-nc-sa/3.0/us
Join us at www.QSTORM.org
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