prokaryotic cell structure and function (part i) prokaryotic cell structure and function (part i)...
TRANSCRIPT
![Page 1: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/1.jpg)
Prokaryotic Cell Structure Prokaryotic Cell Structure and function and function (Part I)(Part I)
BIO3124Lecture #3 (I)
![Page 2: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/2.jpg)
Plasma Membrane Properties and Functions defines the existence of a cell Made of lipid bilayer
Double layer of phospholipids
Surrounds the cell approx. 5-10 nm in thickness
Separates exterior environment from interior Dynamic selective barrier Concentrates certain components intracellulary Allows excretion of waste
Sense the outside world Several metabolic processes
ex. Respiration, photosynthesisex. Respiration, photosynthesis
![Page 3: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/3.jpg)
Fluid Mosaic Model of Membrane Structure
Lipid bilayer in which proteins float (Singer and Nicholson model)
![Page 4: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/4.jpg)
Membrane proteins Membrane proteins serve numerous functions,
including:- Structural support- Detection of environmental signals- Secretion of virulence factors and
communication signals- Ion transport and energy storage
Have hydrophilic and hydrophobic regions that lock the protein in the membrane
![Page 5: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/5.jpg)
Membrane lipids
Amphipathic phospholipids polar ends (hydrophilic)
Glycerol, negative charge (outer leaflet) Ethanolamine, positive charge (inner
leaflet)
nonpolar ends (hydrophobic) Tails of fatty acids Palmitic acid Oleic acid (kinking) increase
fluidity Cyclopropane conversion
aging cells
Phosphatidylethanolamine
![Page 6: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/6.jpg)
Bacterial Membranes differ from eukaryotes
in lacking sterols do contain hopanoids,
sterol-like molecules synthesized from similar
precursors Stabilize bacterial
membranes total mass on earth ~1012
tons
a highly organized, asymmetric structure, flexible and dynamic
![Page 7: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/7.jpg)
Archaeal membranes Etherglycerol, not ester
bond Terpene derived lipids some have a monolayer
membranes Tetra-ether glycerol Cyclopentane: isoprene
cyclization Increased stability
![Page 8: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/8.jpg)
Archaeal membranes
Extreme thermophileseg. Solfolobus and Theromoplasma
Moderately thermophilic- Bilayer or mixed
![Page 9: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/9.jpg)
Role of cell membrane in energy metabolism
• bacterial cell membranes involved in ETC• Gradual energy release• forming proton gradient across membrane
![Page 10: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/10.jpg)
Animation: A bacterial electron transfer system
![Page 11: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/11.jpg)
The transfer of H+ through a proton pump generates an electrochemical gradient of protons, called a proton motive force.
The Proton Motive Force
- It drives the conversion of ADP to
ATP through ATP synthase.
- This process is known as the
chemiosmotic theory.
![Page 12: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/12.jpg)
Besides ATP synthesis, p drives many cell processes including: rotation of flagella, uptake of nutrients, and efflux of toxic drugs
PMF Drives Many Cell Functions
![Page 13: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/13.jpg)
ATP synthase mechanism
Note: pump also works in reverse to create H+ gradient
![Page 14: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/14.jpg)
Cell Transport Transporters pass material
in/out of cellPassive transport follows gradient
of material Pumps use energy
ATP or PMFMove material against their gradient
Passive diffusion lets small molecules into cell
![Page 15: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/15.jpg)
The Bacterial Cell Wall
rigid structure that lies just outside the plasma membrane
![Page 16: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/16.jpg)
Functions of cell wall
provides characteristic shape to cell
protects the cell from osmotic lysis
may also contribute to pathogenicity
very few procaryotes lack cell walls, ie
Mycoplasmas
![Page 17: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/17.jpg)
Evidence of protective nature of the cell wall
Lysozyme treatment
Penicillin inhibits peptidoglycan synthesis
![Page 18: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/18.jpg)
• few PG layers, defined Periplasmic space • unique outer membrane, LPS, Braun’s lipoprotein
Braun’s
![Page 19: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/19.jpg)
• Multiple PG layers, periplasmic space exposed• Teichoic acid
![Page 20: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/20.jpg)
Peptidoglycan (Murein) Structure
Mesh-like polymer composed of identical subunits
contains N-acetyl glucosamine and N-acetylmuramic acid and several different amino acids
chains of linked peptidoglycan subunits are cross linked by peptides
![Page 21: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/21.jpg)
Cell wall unit structures
![Page 22: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/22.jpg)
Bacterial cell wall
G-
G+
![Page 23: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/23.jpg)
![Page 24: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/24.jpg)
Animation: Bacterial peptidoglycan cell walls
![Page 25: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/25.jpg)
Wall Assembly
Cleavage by autolysinautolysin Pre-formed subunits added. Bridges created (transpeptidationtranspeptidation)
![Page 26: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/26.jpg)
Archaeal cell walls lack peptidoglycan
Resemble G+ thick wall
cell wall varies from species
to species but usually
consists of complex hetero-
polysaccharides and
glycoproteinseg. Methanosarcina, and
Halcoccus have complex
polysacharides resembling those of
eukaryotic connective tissue
extracellular matrix
Methanogens have walls
containing pseudomurein
![Page 27: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/27.jpg)
Archaeal cell walls: Pseudomurein
• NAT instead of NAM; links to NAG by β(1→3)glycosidic linkage instead of β(1→4)
•no lactic acid between NAT and peptides
• NAT connects to tetra-peptide through C6instead of NAM C3 in eubacteria• in some tetra-peptide consists of L-amino acids instead of D-amino acids
NATNAG
![Page 28: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/28.jpg)
Capsule (not all species)
Polysaccharide S-Layer (not all species)
Made of protein
Thick cell wall Teichoic acids for strength
Thin periplasm Plasma membrane
The Gram-Positive Envelope
![Page 29: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/29.jpg)
Gram-Positive Cell Walls
CW composed primarily of peptidoglycan
contain large amounts of teichoic acids
polymers of glycerolor ribitol joined byphosphate groups some gram-positive bacteria have
layer of proteins on the surface of peptidoglycan
![Page 30: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/30.jpg)
Capsule (not all species)Polysaccharide
Outer Membrane LPS (lipopolysaccharide)
In outer leaflet only Braun lipoprotein Thin cell wall
one or two layers of peptidoglycan
Thick periplasm Plasma membrane
The Gram-Negative Envelope
Peptidoglycan cell wall
![Page 31: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/31.jpg)
Braun lipoprotein Bridges inner leaflet of
outer membrane to peptidoglycan
67 aa protein with N-terminal Cyc-
triglyceride C-terminal lysine
connected to mDAP by peptide bond
Braun (Murein) lipoprotein
![Page 32: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/32.jpg)
Porins more permeable than
plasma membrane due to presence of porin proteins and transporter proteins
porin proteins form channels through which small molecules (600-700 daltons) can pass
![Page 33: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/33.jpg)
Lipopolysaccharides (LPSs)
consists of three parts lipid A core
polysaccharide O-side chain
(O antigen)
![Page 34: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/34.jpg)
Importance of LPS
protection from host defenses (O antigen variation)
contributes to negative charge on cell surface (core polysaccharide)
helps stabilize outer membrane structure (lipid A)
can act as an endotoxin (lipid A)
![Page 35: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/35.jpg)
Capsules, Slime Layers, and S-Layers
layers of material lying outside the cell wall capsules
usually composed of polysaccharides, some have proteins
well organized and not easily removed from cell eg. Klebsiella and Pneumococcus
slime layers similar to capsules except
diffuse, unorganized and easily removed
a capsule or slime layer composed of organized, thick polysaccharides can also be referred to as a glycocalyx
![Page 36: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/36.jpg)
Capsules, Slime Layers, and S-Layers S-layers
regularly structured layers of proteins or glycoproteins
In bacteria the S- layer is external to the cell wall
common among Archaea, act as molecular sieve letting passage of small molecules
S-layer of Thermoproteus tenax
![Page 37: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/37.jpg)
Functions of capsules, slime layers, and S-layers
protection from host defenses (e.g., phagocytosis)
protection from harsh environmental conditions (e.g.,
desiccation)
attachment to surfaces
protection from viral infection or predation by bacteria
protection from chemicals in environment (e.g.,
detergents)
facilitate motility of gliding bacteria
protection against osmotic stress
![Page 38: Prokaryotic Cell Structure and function (Part I) Prokaryotic Cell Structure and function (Part I) BIO3124 Lecture #3 (I)](https://reader037.vdocument.in/reader037/viewer/2022103123/56649d145503460f949e9223/html5/thumbnails/38.jpg)
Pili and Fimbriae Fimbriae (s., fimbria)
short, thin, hairlike, proteinaceous appendages
up to 1,000/cell mediate attachment to surfaces some (type IV fimbriae) required
for twitching motility or gliding motility that occurs in some bacteria
Sex pili (s., pilus) similar to fimbriae except longer,
thicker, and less numerous (1-10/cell)
required for mating (conjugation) Produced by F+ strains
The fimbriae of P. vulgaris