proposed molecular structure of humic acidtimetraveler.html.xdomain.jp/pdf_yzn/yzn12-3.pdfhumic acid...
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Proposed molecular structure of humic acid
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A D F D ,AH A F CH F DF D DC A BCA F D
, A B A D CAH BA C0 F A D
F AD - A AD C D - AF AD A D A DF CD
CA D 1 D FCA D C BA CA A D
C B A D ,CA F
- CA A D F D A D C D1 D D 1B B
B B B
A B B
- F A BA D C D, BA BCA C A AC BA C F
ADB A B D
+ A C D F A B D
. A A AI A BA C AA A A D D
C D AI D
FC B H D A F DF D D
-C
D
D D C ACA D
-CA
D
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Humic acid
Humin
Fulvic acid
Water soluble OM
Organo-mineral complexes
Free form OM
Humic substance Non-humic
Concept on soil organic matter
Soluble in acid and alkaline
Not soluble in alkali or acid
Precipitate with acid
Bound with minerals
Dark colored, amorphous high polymer OM with known structure
Not bound with minerals
Soluble in water
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COO-
COO-
COO-
COO-COO-
COO-
COO-
COO-
OH
OH
OHOHOH
OH
OH
OH
COO-
COO- COO-
COO-
-OOCOO
O
OO
OO
O
O O
Hydrophilic part
Hydrophobic part
Carbohydrate chain
Condensed ring
Turbostratic structure
Structural concept of humic substances
NH3+
Protein
CO
CO
COCO
CO
CO
CO
OC OC
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Synthetic expression of elementary composition of humic
substances
• As indices for expressing elementary composition synthetically, following ratios are calculated. Elementary number is used in the calculation.
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Combustion Quotient
• Combustion quotient (CQ) is a theoretical value for respiration quotient as proposed by Tamiya 5)
• CQ= 4C / (4C + H�3N�2O) ------ (1)
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Degree of Unsaturation
• Degree of unsaturation ( DU) shows the number of unsaturated bonds and ring bonds per 100 carbon atoms.
• DUH = (2C + N�H) / 2C �100 ------ (2)
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Degree of Oxidation (ω)
ω��2O�H�/ C ------ (3)shows the excess or deficit of oxygen and hydrogen in comparison with Cn(H2O)n
• This value is distributed between �0.8 and +0.9 for humic substances.
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Elementary composition of humic substancesH/C and O/C
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/ /.36/7 637 6 /.36/7 D 63 3. 63 7 3 37 3 / / 3 3. 6 3 7 3
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Classification of humic substances by RF and ΔlogK� Type A, � Type B, � Type P with obvious Pg absorption, �
Type P without Pg absorption� Type Rp from mineral soil, � Type Rp from O layer
Type A
Type B
Type Rp Type P
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UV-vis. Absorption spectra of humic acis in different typesType A Inogashira (volcanic ash soil), Type B Higashiyama (brown forest soil),
Type P Tsubame (Pg of buried soil), Type Rp Anjo (paddy soil)Concentrations are adjusted to 1mgC mL-1
0.1
1
10
100
200 250 300 350 400 450 500 550 600 650 700
absorbance
nm
A�B�P�Rp�
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pH12-pH7 difference absorption spectra of different types of humic acids.�Same humic acids as in the previous figure�
-2
-1
0
1
2
3
4
5
200 300 400 500 600 700
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nm
A�B�P�Rp�