protecting the nci mouse resource: melody roelke-parker, dvm laboratory animal sciences program...
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Protecting the NCI Mouse Resource:
Melody Roelke-Parker, DVMLaboratory Animal Sciences Program
October 2015
NCI Mouse 101
Only limited pathogens are allowed
and Rigorous importation quarantine
Sentinel surveillance
Strict guidelines for order of facility visits
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Biosecurity is in ALL of our hands
“NIH”
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Extremely Valuable resource
~$23 per mouse$118 per cage
$4,800 half Rack (Red Cards)
C57BL/6J mice
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Extremely Valuable resource
C57BL/6J mice
Bldg 10/CRC
$600,000 for
Athymic nudes
10 racks/room$160,000 for B6
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Excluded pathogens
Viruses
Bacteria
Parasites
Imported Mice must be pathogen free
Source of miceSource of mice
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Source of mice
APPROVED SOURCES
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Germantown production facility ONLY
Case-by-case review
Source of mice
APPROVED SOURCES
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Ordering mice• Different for NCI facilities and centralized
facilities managed by DVR• All orders placed by facility staff• List the strain or stock, sex, number, age,
delivery date, facility• Must include PI, current ASP# and Center #• Confirmatory email will be sent to ordering
investigator• Please be sure that name, contact info, and
ASP # is accurate.
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APPROVED SOURCES
Source of mice
Receiving and Quarantine Program at NCI-Frederick
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Receiving Mice• Approved sources are the Charles River Labs,
Jax, & Taconic (Germantown only)• Receiving and Quarantine Program at NCI-
Frederick for most other sources• Quarantine length 3.5-7.5 weeks• Details and online forms at LASP website
http://www.ncifcrf.gov/rtp/lasp/intra/randq/default.asp• Mice may be moved by LASP staff between
compatible facilities• Health status is first reviewed by Facility Vet• Recommended acclimatization: 5-7 days
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Excluded pathogens at NIHHealth status varies with the facility
On the NIH Campus people and mice are allowed to move between facilities with similar health status –
BUT MUST NEVER transit between a poorer health status and a higher one.
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No Pathogens NIAID &Human Genome
10
50
Excluded pathogens at NIHHealth status varies with the facility
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Norovirus LASP37
12th
10
41
DVR
Excluded pathogens at NIHHealth status varies with the facility
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Helicobacter
10A 13th
DVR
LASP 13th
Excluded pathogens at NIHHealth status varies with the facility
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Parvovirus
10A
DVR
Excluded pathogens at NIHHealth status varies with the facility
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Pinworms & fur mites
DVR
Excluded pathogens at NIHHealth status varies with the facility
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Norovirus
Helicobacter
Parvovirus
Pinworms & fur mites
No Pathogens NIAID &Human Genome
LASP
DVR
Excluded pathogens at NIHHealth status varies with the facility
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Norovirus
Helicobacter
No Pathogens
LASP10A 13th
DVR
10
Parvovirus
Excluded pathogens at NIHHealth status varies with the facility
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Norovirus
Helicobacter
No Pathogens
LASP
DVR
37
Parvovirus
Excluded pathogens at NIHHealth status varies with the facility
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Excluded pathogens at FrederickHealth status varies with the facility
NCI Frederick Campus
Receiving & Quarantine
Norovirus
Helicobacter
No PathogensRepository, & Transgenic Mouse Model Laboratory
Only 2 are positive
Dr. Braden-Weiss can provide
Frederick facility status
Most are positive
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Tumor Cell Line Testing Requirements
• PCR-based testing has replaced MAP-testing to a large extent (MTBM - Molecular testing of Biological Materials)
• Instructions at LASP AHDL web page• All untested cell lines if they have been
passaged through rodents outside of the NCI animal program or exposed to rodent serum or if their history is unknown should be submitted
• Human-derived cells
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Tissue culture & reagents potentially infected with rodent
pathogensMolecular Testing of Biological Materials
(MTBM)
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Taking mice to the Laboratory
• Special routes and elevators, esp. in CRC
• Should be rapid and direct
• Provide food, bedding, fresh water source
• Practice containment and minimize stress to animals
• Unless the contrary is approved in the ASP, animals must be euthanized within 12 hours or less
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Custom Diet Formulations
• Rodent Feed producersZeigler, Purina, Harlan, Research DietsBio-servOthers
• Must be sterile by gamma-irradiation or autoclavable
• Consultation with a diet formulation specialist & facility veterinarian is advisable for new test compounds
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Sentinel Monitoring Program
Sentinels
Every week, sentinels are exposed to
dirty bedding from all
cages on rack
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Sentinel Monitoring Program
Sentinels
Every 6 weeks, each sentinel cage is tested for a
panel of excluded
pathogens
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Sentinel Monitoring Program
Sentinels
Twice a year, a necropsy
with cultures is performed
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Excluded pathogensHealth status varies with the facility
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Consequences of contamination..
• Alteration of experimental measures, tumor growth rates, immunity, etc.
• Quarantine will limit access to animals• Movement of animals ceases• Procedure rooms, MIF, etc. may not be used• Expensive testing ; expensive extra PPE• Cull and slowly repopulate with clean stocks• Reduced ability to share mice with colleagues• Research progress and productivity may be
severely affected
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SHIPPING MICE ON NIH CAMPUS• ON NIH: File out an Internal NIH/IC/
Rodent Transfer request.
• Must contact receiving facility veterinarian and facility manager to obtain shipping authorization
• Some facilities may required special testing, others will not accept NCI mice.
• Most IC’s will accept LASP animals
• LASP requires all Non-Approved NIH animals to go to R&Q
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• ALL NON-Approved sources MUST go through Receiving and quarantine in Frederick (This includes ANY non-LASP facility at NIH)
• Fill out on-line importation request
• https://ncifrederick.cancer.gov/rtp/lasp/intra/randq/forms/import_nonapproved.asp
• Must obtain Complete information from sending facility – including health documents and contact information
• Contact Sandra Warfield with any questions
IMPORTATION OF MICE
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• What to do if your mice test positive for Excluded agents while in Quarantine?
• Best for LASP facility veterinarians to evaluate health report of sending institution BEFORE mice are shipped. Then you are forewarned of probably Positive results.
IMPORTATION OF MICE
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• If Positive – must rederive before you can import into LASP facilities – OR
• Send to the ACRF 13th floor or a DVR facility of appropriate health status
IMPORTATION OF MICE
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Very Challenging – Esp. International shipments
• Every Country is different – US Domestic shipments are relatively uncomplicated
• Must obtain complete contact and address information from receiving institution facility –
EXPORTATION OF MICE
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• Requirement for MTA (Material Transfer Agreement)
• Set up Accounts with FedEx Express and
EXPORTATION OF MICE
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• Contact Jodi Penn or Dr. Melody Roelke-Parker for assistance with all outgoing NIH shipments.
• Ship only young, healthy animals• You are ultimately responsible for the mice
selected for shipping.
Make sure that the ID and SEX is correct, and that the cages are properly marked
EXPORTATION OF MICE
START EARLY – CAN TAKE 1 OR MORE MONTHS
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Questions?