8/8/2019 Proteins Outline

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I. Proteinsa. Nitrogen Contentb. Charge and Isoelectric Pointc. Shaped. Solubility

e. Immunogenicityf.Protein Structureg. Denaturation

II. Classification of Proteins (based on composition)a. Simple Proteinsb. Conjugated Proteins

III. General Functions of ProteinsIV. Plasma Proteins

a. Prealbuminb. Albuminc. Globulins

d. Α-1 antitrypsine. Α-1 acid glycoproteinf. Α-1 fetoproteing. Α-2 macroglobulinh. Haptoglobini. Ceruloplasmin j. Hemopexink. Transferrinl. C-reactive Proteinm. Fibrinogenn. Immunoglobulins

1. IgG2. IgM3. IgA4. IgE

V. Miscellaneous Proteinsa. Myoglobinb. Troponin

VI. Quantitaion of serum total proteina. Kjeldah Methodb. Biuret methodc. Dye Binding Method

d. Ultraviolet AbsorptionVII. Quantitation of specific proteins

a. Refractometryb. Salt Fractionationc. Dye Bindingd. Determination of Total Globlins

VIII. Total Protein Abnormalitiesa. Hypoproteinemia

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