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PRP (Platelet-Rich-Plasma) TherapyA presentation

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• BS PRP System• No need to use needles for

extracting PRP.• Minimize exposure to

air contamination• State-of-the-art extraction skills

based on patents• PRP (Platelet-Rich-Plasma)

Therapy

Patents



Patents

The Characteristics of BS PRP System

• A) State-of-the-art skills based on patients.

• B) Minimized exposure to air contamination.• No need to use needles to extract PRP• Need only 20cc blood to extract PRP• Time saving with simple extraction process• Very easy process available to all medical staff• The movement of Buffy-coat which is floating inside

plasma can be observed with the naked eyes

It is amazing!


TIGER WOODS & PRP‘He claims that the treatmentswere successful and he Feels strong’

The New York Times – Feb 16 2009A promising treatment for Athletes, in BloodGroundbreaking platelet-rich plasma therapyprovides highly innovative treatments for athletes.Hines Ward of the Pittsburgh Steelers is among thefirst recipients of Plasma Therapy to treat a sprainedmedial collateral ligament in his right knee.

Examples of PRP prolotherapy


PRP (Platelet Rich Plasma) Therapy


What is PRP?Platelet is one of blood elements. It usually moves alongthe blood stream as inactivated form. If it is activated byWounds or damages of tissues, it does perform itsoriginal functions of bloodclotting and wound healing. PRP therapy is to maximize the main functions of platelets.

PRP (Platelet Rich Plasma) Therapy



STEP 1Blood Sampling

After drawing blood,shake syringe well inorder to Mix PRP And Anticoagulant

PRP (Platelet Rich Plasma) Therapy step-by-step

STEP 2The value forCentrifugation

1000 RCF (about2500 rpm) 9 minutes.(use only a soft spin)The spin value mayBe different accordingto the ages or sex.

STEP 3The figure after Centrifugation

Red blood cells, buffyCoat, plasma frombottom to top.

STEP 4Extraction of PRP

There is no need to useNeedles. PRP Kit.Use only <syringe tosyringe movements>



Applications for PRP

• There are a lot of reports about the efficacy of PRP, and the effects of PRP have been proved already through many scientific studies

• PRP is a fundamental and excellent treatment method for chronic diseases which are not cured easily, and is a noninvasive method which needs no surgical interventions.

After6 days

After6 months




1. Aesthetic purpose, bald treatment, pain control

2. Degenerative arthritis3. Osteomalacia4. Cartilage injuries5. Ligament injuries6. Frozen shoulder7. Chronic elbow tendinosis (tennis

elbow, golf elbow)

8. Ligament-injuries according to various Sports (sport medicine)

9. Ligament injuries or cartilage damage of ankles

10. Achilles tendinitis11. Plantar fasciitis12. Pain control after operation13. When fast recovery is required

after operation



The movement of Buffy-coat I



The movement of Buffy-coat II




• Extraction of PRP with Buffy-coat

• PRP extraction process




• Separated PPP, PRP




Immediately aftercentrifugation

After time passed

Platelet



BS PRP System is a kind of ‘SUPER PRP Kits’

BS PRP SystemFor effective PRP treatment, platelets should be keptIn their original conditions (they should not be activatedEarly stages, but collected in unimpaired conditions).



Showed picture in microscope (Original)



Platelet Count Test Record

Examined in the laboratory of Cell & Tech Corp.BS PRP System



Hematopoietic Stem Cell Pathway



Hemostasis process

At first step, thrombin converts fibrinogen to fibrin

Finally they form a clot

with platelets and leukocytes

Then, Fibrins form 3 dimensional structure when fibrins get together ( thereby they form fibrin mesh )

Fibrin thread

Let’s see about normal hemostatic process.



Sequencing physiologic changes after introducing PRP in our body

1. Fibrins form 3-dimentional structure

2. Various growth factors are secreted from platelets and

leukocytes 3. GFs cause migration of stem cells

5. Stem cell differentiation 4. Stem cell proliferation

These pictures are showing the sequencing physiologic changes after introducing PRP into our skin.



Sequencing physiologic changes after introducing PRP in our body

Injection of activated platelets in the skin.

Growth factors emitted fromPlatelets draw stem cells.

Stem cells create elasticFiber cells like collagen

and elastin.



PRP 20 %

PRP 0 , 2%FBS

PRP 2 %

PRP 0 %

Fibroblast under various conditions for 4 days (in vitro)



Platelet• Size:2–4 micometer• Consist:

£Seudopodial extensions£Vaginations£Types of internal vesicles

• 3 types of internal vesicles®Aysosomal vesicle®Dense vesicle®Alpha vesicle

What is PRP?

Stable state

Activated state



Activation–1


Definition:Activation means to change stable (inactivated)platelets to activated platelets, thus variousgrowth factors are easily released from inside of platelets.

Activation–1



This picture is showing the difference between stable platelet and activated platelet. There are several growth factors inside alpha-vesicle.When platelets are activated, alpha vesicle pours out a lot of growth factors immediately.

Activation–1 Stable platelet & activated platelet



This picture is showing that activated platelets are only possible to release several growth factors.

Effectiveness

Activated Platelets release Growth FactorsInto the surrounding tissue.

Measuring available platelets may be a measure of available protein load in The wound site.

Growth FactorProtein



Role of growth factors

Several GFs are involved in several inflammation and wound healing process



What happens as we get older?

When we are young, there are thick healthy dermis, in which a lot ofCollagens and elastins reside, but as years go by, our dermis loses its

Healthy dermis, thus there remains little collagens and elastins.

24 years old 48 years old

Elastica von Giesson staining



Activation–2


PAP-A : Safe Activation


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Safe Activation of Platelets

PRP - A RESULT

= More effective than higher cost Thrombin

= Calcium Chloride / Chemicals promotes Side Effects / Allergies

Invented in South Korea and possesses International patent

Simple device (1 kit )

Based on statistics and studies, PRP-A promotes the quickest wound Healing results




Activation Factor Chemical agonist Physical stress

Type

Calcium Chloride, Thrombin, ADP, Collagen, Peptide , etc.

- Shear stress

-Capillary stress

Advantages Can activate platelets by simply adding agonist- Safety

- Overcame adverse effects of chemical substances

Drawbacks- Possible thrombus formation due to strong blood

clotting stimulation- Impossible clinical apply except Calcium Chloride

No commercialized product in the market

→ World’s first



PhysicallyActivated

PRP using PRP - A

ChemicallyActivated

PRP• Promotes high possibility of allergic

reactions

• Promotes high possibility of side effects

• Chemically altered procedure (non-autologous procedure)

• Higher count of Growth Factors (EGF, VEGF, FGF-2, PDGF etc.)

• Full “100% autologous PRP” procedure

• Safe and easy procedure with a small device using capillary stress to enhance growth factors in platelets


PAP-A : Clinical Data

Natural Healing

PRP

A-PRP

In-vivo Validation Evaluation (Wound Healing Model)

Wound Healing Assay

WB PRP PRP + THR PRP + C S


PAP-A : EGF

Epidermal Growth Factor

PRP‐A

Epidermal Growth Factor, or EGF, is a growth factor that stimulates cell growth, proliferation, and differentiation.


PAP-A : PDGF

Preliminary Experiment: PDGFPlatelet Derived Growth Factor, or PDGF, is a growth factor that plays a role in embryonic development, cell proliferation, cellmigration and has been implicated in tissue remodeling and cellular differentiation.


PAP-A : VEGF

Vascular Endothelial Growth Factor: VEGFVascular Endothelial Growth Factor, or VEGF, is a growth factor that plays a part in restoring the oxygen supply to tissues when bloodcirculation is inadequate.


PRP Applications


Ageing process

As we get older year by year, our skin nature also gets some changes.In early 20s, there are no wrinkles, but as we get older, there appears some

Changes in our skin, such as many wrinkles, dark circles etc

This picture is showing the changes of skin with age (20s–60s)



PRP used to treat impaired skin

A B

A

B After 20 days of PRP treatment

After 30 days of common treatment



Surgery for the chin and face

Autologous bone from Iliac crest

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Other Surgery

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Figure 4: Patient nr 2 (Table 2). A neuroischemic diabetic heel ulcer prior to treatment with platelet gel.

Figure 5: Day 27: the wound size is reduced by 40%

Figure 6: Day 155: The wound has healed after two applications of platelet gel, off-loading and good local wound care.



Other Surgery

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(After 6 days)

(After 6 months)



Chronic ulcer treatment

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A: 83/F (3 months in the hospital)Before PRP treatment in the hospital

B: After seven PRP treatments for 160 days (Discharged from hospital after first PRP treatment.



PRP aesthetic applications

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Wrinkle Melasma Freckles Loosened skin

PRP can be used for treatment of wrinkles, melasma, freckles and loosened skin.




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Before surgical procedure

After surgical procedure




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Acne, Whitening

before after




Bio membraneAcne, Whitening

before after




Bio membraneAcne




Bio membraneAfter PRP treatments (4 times)



Pain Treatment


PRP Prolotherapy (Comprisal table)

Bio membraneImpaired ligaments and muscle



Clinical application Orthopedic, sports medicine

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• Tendinosis• Acute and chronic muscle strain• Ligamentous sprain• Joint capsular laxity• Arthritis• Articular cartilage defects• Meniscal injury• Chronic synovitis




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Figure 1. Platelet-rich plasma injection technique




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2. Should be injected in the correct partA. Surgical operation under ultrasonic wavesB. Surgical operation, seeing C arm




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Correct injection on theimpaired part under Ultrasonic waves




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Figure 6.Musculoskeletalultrasound, commonextensor tendinosis

Figure 7.Ultrasound guidedsuprapatella bursainjection/graft

Figure 8.Ultrasound guided kneeMCL injection/graft

Correct injection on the impaired part under Ultrasonic waves




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Regeneration of damaged ligaments

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Before After



Regeneration of damaged ligaments

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The World Anti-Doping Agency (WADA)

In 2010, WADA permitted for the athletes to usePRP Prolotherapy for tissue regeneration.



Celebrity/Media endorsements

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Hines Ward: American Football

Date of Birth: 8 March 1976

Height: 183cm Weight: 98Kg

Eldrick Tiger Woods: Golf

Date of Birth: 30 December 1975

Height: 185cm Weight: 84Kg



Celebrity/Media endorsements

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Comparison of Magellan and yes PRP

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BS PRPAextraction ways electronic manualStart up costs (USD 18,000) (USD 900)Disposal kit price: (USD 150-450) (USD 30-40)Extraction order: RBCPRPPPP PPPPRPRBCWays: by using density difference by using density difference

Extraction time: fifteen minutes eight minutesExtracted platelet number: max 2-2.5 million/ul max 2-2.5 million/ulRBC inclusion volume: many modification possibleContaminaton possibility: Less LessCentrifugation No: one time one time



Comparison of Magellan and yes PRP

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Thank You



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