australian proteomeanalysis facility

PTMs - Glycan AnalysisAPAF - AUSTRALIA’S PREMIER PROTEOMICS PROVIDER SINCE 1995 APAF’s mission is to assist the scientific community address their protein analysis needs. Australian Government support has enabled APAF to create a world-leading proteomics analysis facility containing the latest infrastructure for advanced protein analysis. Our strengths in comparative analysis, along with absolute protein quantitation, have increased our capabilities to deliver leading edge proteomic services.

Glycan analysis explainedGlycosylation is one of the most universal and structurally diverse post-translational modifications (PTM) with more than half of all mammalian proteins predicted to be glycosylated.

The significance of correct glycosylation in various biological events such as protein secretion, stability and immunogenicity and the direct correlation of aberrant glycosylation with diseases like cancer and congenital disorders of glycosylation are well documented. Identification and characterisation of glycans together with their particular protein carriers is crucial for deciphering glycan functionality in normal and diseased conditions.

APAF has a documented record of glycan analysis with leading biotechnology firms.

APAF’s services:• Release of N-Linked glycans

(using PNGase F) and O-Linked glycans

(using beta-elimination) from purified

proteins for comparative profiling using

carbon LC/MS/MS

• Glycan structural analysis using

Exoglycosidase and MS/MS fragmentation

• N-Linked glycopeptide analysis of lgG

antibodies using reversed phase LC/MS/MS

• Monosaccharide analysis of purified

eukaryotic glycoproteins using HPAEC-PAD

WE DELIVER LEADING

EDGE PROTEOMIC

SERVICES

We provide services to:

• Research Institutes

• Universities

• Pharmaceutical R & D

• Biotech R & D

Glycopeptides IgG

Our expertise includes:

1. World class expertise in

glycobiology

2. Leading edge

mass spectrometry

instrumentation

3. Established methods for

glycan characterisation

4. Development of QC

methods for recombinant

protein/antibody batch

control

ENQUIRE NOW!E: lwoods@proteome.org.au F: +61 2 9805 3101 skype. apafbdmT: +61 2 9805 3175 M: +61 416 250 739 APAF - Australian Proteome Analysis Facility Ltd. Level 1, 3 Innovation Rd Macquarie University Sydney NSW 2109 Australia

www.apaf.com.au

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APAF - Australian Proteome Analysis Facility Ltd. Level 1, 3 Innovation Rd Macquarie University Sydney NSW 2109 Australia

www.apaf.com.au

australian proteomeanalysis facility

Proteomics assisting your biological research

APAF works with scientists from small academic laboratories to the largest pharmaceutical companies in the world. We develop new techniques and work with state of the art published techniques. Whenever we develop new techniques or deploy new infrastructure we showcase this in our e-newsletters. Our e-newsletters and journal publications can be viewed on our website.

Services offered at APAFDiscovery and Comparative Techniques

• iTRAQTM • SILAC• Label free LC-MS/MS • Di-sulphide bond analysis• Phosphoproteomics • Free amino acids in physiological fluids• Amino thiols • DIGE

Targeted Techniques

• SRM • MRM–HR on TripleTOF 5600 • Multiplex bead based assays • Identification of proteins from gel bands• co-IP and Mass spectrometry • Chemical proteomics• N-Terminal Sequencing • Amino acid analysis for the food industry• Glycan characterisation of antibodies

and recombinant proteins

Other Proteomic Services

• Assay development with HPLC • Protein purification• Abundant protein depletion • Amino acid analysis • Proteogenomics –

correcting genome annotations

Our State of the Art Infrastructure

• Thermo Orbitrap Velos Elite with ETD • 3 x AB SCIEX Triple TOFTM 5600 Systems • 2 x AB SCIEX 5500 QTRAP • Thermo Q Exactive• Agilent QTOF 4960 • 14 Mass spectrometers• 10 Ultra high pressure

chromatography systems

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