Purdue University

Ann Rundell, Assistant Professor

Workshop on BME Teaching of Innovation, Design &

Entrepreneurship

Program ObjectivesNew Undergraduate Program, Fall 2004

• Teach engineering science, analysis, and design in the context of biology and biomedical problems

• Immerse students in key life science principles early

• Integrate biology and engineering within courses

• Emphasize problem-solving, design, and teamwork

• Allow for senior-level specializations• Incorporate ABET considerations

throughoutPurdue University

• Become familiar with the classes of molecules that form the cellular components of living organisms (biomolecules) – sugars, lipids, proteins, and nucleic acids

• Relate structure and function of these important classes of molecules

• Hydrogen-bonding, hydrophobic forces, electrostatic interactions along with other weak interactions

• Understand how these concepts are integral to engineering, controlling and manipulating biomedical platforms

Purdue University

Biomolecules: Structure Function and Engineering Applications

• Laboratory will teach and introduce key skills, techniques, and analysis tools to support the cells and biomolecules courses

Purdue University

Biomedical Engineering Laboratory I:Exploring Biomolecules and Cells

• Module 1: Fundamental Quantitative Techniques• Module 2: From Biomolecules to Cells: Isolation,

Identification and Quantification• Module 3: Analysis of Integrated Biosystems• Module 4: Application and Design of Biomedical

Platforms

EngineeringFundamentals

BiomoleculesConcepts

CellBasics

BME Lab I

Describe particularly novel and/or effective methods you use to teach innovation &

entrepreneurship• Implement series of mini-design projects

throughout curriculum with capstone design experience senior year

• Sophomore Mini-Design Project – DNA and Protein Chips– Students demonstrate fundamental principles

of biosensor design – Design to be tested via simulation prior to

fabrication – Build DNA or Protein Chip in laboratory and

test

Purdue University

How do you measure outcomes?

• Design: – Formal design reviews with invited industrial

representatives– Student demonstrations of working aspects of design

projects • Concepts:

– Virtual Laboratory on Web• A virtual laboratory bench with all of the equipment available

in the lab• Students will be presented with a task and must identify

equipment used for task at hand, provide a reason for its inclusion, sequence the steps to perform the task

• Provides immediate feedback with ability to iterate until the student successfully completes the task

• Provide feedback to instructor on how long it took the students to complete the assignment, how many iterations it took, and compile a list of common problems

– Written assignments: Homework, quizzes and exams

Purdue University

Describe a course project or exercise that exemplifies “best

practices”• Sophomore Lab on Polymerase chain reaction (PCR)• Introduces primer design, PCR amplification, and

quantitative measurements of nucleic acid material – Prelab:

• Rank selected oligonucleotide primers by their optimal annealing temperatures using computer programs.

• Predict the quantity of nucleic acid material present after different numbers of cycles.

– Lab: • Determine the composition of unknown samples of nucleic

acid material by applying their knowledge of primer design, benchtop PCR thermocyclers, agarose gel electrophoresis, and UV spectroscopy.

• Primer design, PCR, and quantitative analysis of genetic material will be essential skills for future biomedical engineers for the manipulation and analysis of genetic material

Purdue University