purification column guide - sepsolutions interchim/interchim... · purification column guide 1 ......

PurificationColumn Guide• Medicinal Chemistry • Natural Products • Proteins & Peptides

• Normal phase• Reverse phase• Ion exchange• Chiral

www.interchiminc.com

Flash

Prep HPLC

40g Conventional Flash Column

1.0 gram sample, pure fractions 1.25 gram sample, mixed fractions

Solvent savings and higher loading based on purifying the equivalent amount of sample on a conventional flash column. % Loading: Sample / silica (w/w)

100% Purity

25g PuriFlash HC15 3.0 g 17 367 ml 460 ml40g Conventional 1.0 g 11 263 ml 480 ml

Sample Load

Collected Volume

Solvent ConsumedTubesColumn

Test ConditionsSample: 500 mg/ml dibutyl and diethyl phthalateFlow rate: 20 ml/minSolvents: A: Heptane B: EtOAcGradient: 0 - 7 CV 5% B, 7 - 13 CV 5 - 8% B

UV: 254 nmTube volume: 25 ml

Partial Purity / Overload Condition

Sample Load

Collected Volume

Solvent ConsumedTubes

Column

40g Conventional 1.25 g 13 298 ml 540 ml

UPFP reduces the purification time and cost per sample with increased throughput and confidence. The small particle spherical silica provides significant benefits when compared to conventional flash grade silica. UPFP columns greatly improve separation efficiency and resolution which allows for higher loading, faster through-put and reduced solvent consumption.

In the example above, the narrow bands on the UPFP column suggest that much higher loading could be achieved while still maintaining purity. Below, a 25 gram UPFP column outperforms the leading 40 gram conventional flash column.

25 gram UPFP Column

500% higher

loading

70% less solvent

300% less collection

volume

3.0 gram sample, pure fractions

Contact Interchim for more [email protected] • 800.560.8262

www.interchiminc.com

1Purification Column GuideMedicinal Chemistry • Natural Products • Proteins & Peptides

TLC to Flash Optimization Chart Back Cover

Alumina — ALN: Neutral, ALB: Basic

Column Hardware Physical Specifications

3, 10

28

Dry Load — Empty or Partial Fill: Celite, Silica 3, 11Carbon Columns for Decolorization Web

Ion Exchange — SCX, SAX, MM1 5, 18

ColumnsSilica — HC: High Capacity, HP: High Performance 3, 8, 9, 27

Reverse Phase — C18XS, C18HC, C18HP, C18HQ 4, 10, 19-20

Reverse Phase High Aqueous — RPAQ, C18AQ 4, 21Reverse Phase Polymers — Atoll X, 302H 4, 20

Cyano, Diol, HILIC, DEAP, NH2 3, 12, 17

Cellufine — Ion Exchange, Affinity, HIC, Endotoxin Removal 6, 25-26Reverse Phase Bonded Silica— C4, C8, C18 (200 & 300Å) 6, 22-24Biopurification

Prep HPLC ColumnsChiral Purification 13

TLC Plates Back Cover

14

Instruments for Flash and Prep HPLC 29

Natural Products — P6 4, 18

General GuidelinesColumn Selection Chart

22

Column Selection Guide 3-6

Resources

TLC Optimization 7

Applications 15-16

For more pricing options, go to www.interchiminc.com

2 Column Selection Chart

Sample Column Selection

Charged

Acidic

Basic

Low to MidPolarity

Acid Sensitive

HighPolarity

Reverse PhaseC18HP, C18HC

C18HQ, CN

Reverse PhaseC18HP, C18HCC18HQ, CN, P6

Reverse PhaseRPAQ, Diol, 302H,

NH2HC, DEAP

Reverse PhaseC18HP, C18HC

C18HQ, CN, NH2HCALB, MM1, DEAP

Reverse PhaseC18AQ, C18HP, X

C18HC, C18HQ, CN, NH2HC, MM1, DEAP

Normal PhaseDiol, CN, ALN

Normal PhaseHILIC

Normal PhaseSIHC, SIHP, ALN

Normal PhaseDiol, CN

Normal PhaseSIHC, SIHP, ALN,ALB, CN, NH2HC,

DEAP

Normal PhaseSIHC, SIHP, ALN,

Diol, HILIC

Reverse PhaseDiol

Ion ExchangeSCX, MM1,

NH2HC, DEAP

Ion ExchangeSAX, NH2HC,

DEAP

Ion ExchangeMM1

Normal Phase: In normal-phase chromatography, the stationary phase is polar and the mobile phase is less polar. The more polar the solvent, the greater the elution strength. To determine optimum conditions, please refer to TLC Optimization on page 7. Includes: SIHC, SIHP, ALB, ALN, NH2HC, NH2, DEAP, HILIC, DIOL, CN

Use: It is possible to re-use puriFlash HC & HP columns by flushing with 100% methanol after each run and then completely remove the methanol by purging with air.Storage: Store in 100% propanol after purging with methanol and air. Do not use heptane or hexane as it will soften the polypropylene column hardware. Replace end caps to prevent drying.

Reverse Phase: In reverse phase chromatography, the stationary phase is non-polar or weakly polar and the mobile phase is more polar. A less polar solvent has higher elution strength. Combinations of water with either methanol or acetonitrile are most often used for gradient elution. Compared to normal phase, reverse phase provides higher efficiency and improved selectivity but lower sample loading capacity. Includes: C18HC, C18HP, C18HQ, C18AQ, RPAQ, 302H, X, CN, NH2HC, NH2, MM1, ALB, P6, DEAP

Use: To prevent out-gassing, always equilibrate in 100% organic. It is possible to re-use puriFlash reverse phase columns by flushing with 100% organic after each run.Storage: Remove organic modifiers, buffer salts and flush with 75% Methanol or Acetonitrile in water with end caps in place.

Ion Exchange: In ion exchange chromatography, the stationary phase is bonded with ionic functional groups that retain the solute ions of opposite charge. Includes: SCX, SAX, MM1, NH2HC, NH2, DEAP

To Retain: Anion Exchange - pKa of the target compound < pH < pKa of the sorbent Cation Exchange - pKa of the sorbent < pH < pKa of the target compoundTo Elute: Anion Exchange - pKa of the sorbent < pH < pKa of the target compound Cation Exchange - pKa of the target compound < pH < pKa of the sorbent Storage: Remove buffer salts by flushing with water and store in 100% Acetonitrile with end caps in place.

General Guidelines

3PuriFlash Column Selection Guide

Type Application Capacity Base MaterialpH

StabilityParticleSizes Page

NH2HC Polar amine compounds with reduced amine-silanol interaction. Suitable for carbohydrates. High Capacity, for higher loading. Eliminate the use of TEA (triethylamine) for basic compounds.

7% High Capacity Spherical Silica 1.5 - 6.5 50µ

NH2 Polar amine compounds with reduced amine-silanol interaction. Suitable for carbohydrates. Eliminate the use of TEA (triethylamine) for basic compounds.

5% High Performance Spherical Silica 2 - 6.5 30 & 50µ

HILIC Very polar water soluble compounds; dyes, polyphenols, nucleotides and alkaloids using aqueous solvent systems. Reduce the use of chlorinated solvents using 100% MeOH or H2O. Reduced rota-vap time by using less water.

7% High Performance Spherical Silica 1.5 - 7.5 30µ

DIOL Enhanced selectivity and higher retention for low to medium polarity compounds vs. bare silica. 6% High Performance

Spherical Silica 1.5 - 7 30 & 50µ

CNMid range polarity between bare silica and C18. 6% High Performance

Spherical Silica 1.5 - 7 50µ

DEAP DiethylAminoPropyl bonded silica. A less polar alternative to NH2. Compatible with MeOH and water for use in normal or reverse phase.

Granular Silica 60µ

Normal Phase, Bonded Silica

Page 1 of 1

Normal Phase, Bonded Silica



SIHC Non-ionic polar organic compounds. Methanol stable, high loading capacity and high resolution. 20% High Capacity

Spherical Silica 1.5 - 6.5 15, 25 & 50µ

SIHP Non-ionic polar organic compounds. Methanol stable, high separation efficiency at high flow rates. 10% High Performance

Spherical Silica 1.5 - 6.5 15, 30 & 50µ

ALB Basic compound purification. Eliminate the use of TEA (triethylamine) to avoid streaking or tailing under normal or reverse phase conditions.

10% Granular Basic Alumina 1 - 12 45µ

ALN Acid sensitive compounds that would degrade if exposed to normal phase silica. 10% Granular Neutral

Alumina 1 -12 45µ

Dry-Load Hand tight, no adapters, stable to 15 bar / 215 PSI NA Silica or Celite 1.5 - 6.5 50µ Carbon Decolorization, no exposure to carbon dust 10% Activated Carbon 1 - 14 50µ

Normal Phase, Bare Silica Alumina and Dry-Load

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Normal Phase: Bare Silica, Alumina, Dry-Load and Decolorization

O Si OH

HC, HP Silica

O

Al

AlO

O Na

Na

Alumina Basic

Al

O

Al

O

Alumina Neutral

O

O

OH

Si

Si

CH3

(CH2)3

CH3

HH

CH3

N

NH2 & NH2 HC

O

O

OH

Si

Si

CH3

(CH2)3

CH3

C

CH3

N

CN HILIC

O

OH

Si (Cx)x

+O O

OH

OH OH

OH

Si (CH2)3 CH2 CH2CH

Diol

8, 27

9, 27

10

10

11

Web

17

17

12

12

12

174%

4

Page 1 of 1


StabilityParticle Sizes Page

C18HP Non polar and ionic compounds. Routine purification. 5% High Performance Spherical Silica 1 - 7.5 15, 30 &

50µ

C18HC Non polar and ionic compounds. Increased surface area for high sample loading. 8% High Capacity

Spherical Silica 1 - 7.5 15 & 30µ

C18HQ Non polar and ionic compounds. Lower cost alternative to reverse phase Prep HPLC. 5% HPLC Prep Grade,

Spherical Silica 1 - 7.5 15µ

C18AQ Mid polar compounds. Start gradient with 100% water. 5% High Performance Spherical Silica 1.5 - 7.5 15 & 30µ

C18XS pH stable from 1-10, basic compounds 21% High Performance Spherical Silica 1-10 15 &

30u

RPAQ Polar compounds. Start gradient with 100% water 5% High Performance Spherical Silica 1.5 - 7.5 15 &

30u

DEAPDiethylAminoPropyl bonded silica. A less polar alternative to NH2. Compatible with MeOH and water for use in normal or reverse phase.

4% Granular Silica 60µ

302H Hydrophilic/hydrophobic polymer for polar and non polar compounds. MW <3 kDa. High capacity reverse phase. 25% SDVB Polymer 1 -13 30 & 70µ

Atoll X Atoll X is a universal polymer for mid & non polar compounds, MW <3 kDa. High capacity reverse phase. 25% SDVB Polymer 1 -13 40µ

P6

Amide functionality for aromatic and natural products; flavonoids, chalkones, anthraquinones, aromatic nitro compounds, DNP amino acids, phenols, carbonic acids, acid amides, sulphonic acids and amides of sulphonic acids and amines.

5% Polyamide 1 - 8 50µ

Reverse Phase Bonded Silica and Polymers

O

OH

O

Si (CH2)3 (CH2CH3)2NH+

DEAP

PuriFlash Column Selection Guide

C18HP, C18HC, C18HQ & C18XS

O

O

OH

Si CH3

CH3

CH3

Si C18H37

CH3

CH3

C18AQ

O

O

OH

P

Si C18H37

CH3

CH3

RPAQ

O P

O

OH

Si CnHn2+1

CH3

CH3

Atoll X

CH2 CH2

CH2

CH2

CH2

CH

CH

CH

C

OO

OO

302H

CH2

x y

CHP

O

P6

(CH2)5

n

C

O

HN

The following phases can also be used for reverse phase: CN, NH2, ALB (page 3) and MM1 (page 5)

19

19

20

21

10

21

17

20

20

18

5



SCX Sulfonic acid functionality which retains weak basic compounds that have one or more positive charges. Remove or isolate basic compounds.

0.3 meq/g High Performance Spherical Silica 1 - 7.5 50µ

SAX Quaternary amine functionality which retains strong (pKa <1) or weak acids (pKa >2). 0.3 meq/g Granular Silica 1.5 - 7 50µ

MM1 Unique selectivity of basic, charged and non-polar compounds via ion exchange and hydrophobic chains (SCX/C8). Compounds that possess basic functionality are retained by ion exchange. A washing step with an appropriate pH, elutes ionizable compounds. Retained compounds from hydrophobic bonding are eluted with an organic solvent.

0.09 meq/g High Performance Spherical Silica 1.0 - 7.5 60µ

NH2HC Weak anion exchange for strong acids 0.3 meq/g High Capacity Spherical Silica 1.5 - 6.5 50µ

NH2Weak anion exchange for strong acids 0.3 meq/g High Performance

Spherical Silica 2 - 6.5 30 & 50µ

DEAP Weak anion exchange (secondary amino, pKa 10.5) 0.3 meq/g Granular Silica 60µ

Ion Exchange, Bonded Silica

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Ion Exchange, Bonded Silica

O

O

O

O-H+S

OH

OH

Si (CH2)3 O

SCX

O

OH

OH

Si N+(CH2)3 (CH3)3

SAX


O

O

O

O-H+S

OH

OH

Si (CH2)3 O

MM1

Si CnHn2+1

CH3

CH3

18

17

18

18

17

17

O

O

OH

Si

Si

CH3

(CH2)3

CH3

HH

CH3

N

NH2 & NH2 HC

O

OH

O

Si (CH2)3 (CH2CH3)2NH+

DEAP

6



90A500(DEAE) Weak anion exchange, intermediate purification of

proteins, peptides and enzymes up to 500 kDa.>60 mg/ml

BSA Cellulose Beads 1 - 13 90µ

90Q500(QA) Strong anion exchange, intermediate purification of

proteins, peptides and enzymes up to 500 kDa. >10 mg/ml

BSA Cellulose Beads 2 - 12 90µ

90C500(CM) Weak cation exchange, intermediate purification of

proteins, peptides and enzymes up to 500 kDa. >70 mg/ml Lysozyme Cellulose Beads 1 - 13 90µ

75SO4(Sulfate) Affinity purification of Virus and heparin binding

protein up to 3 kDa. >3 mg/ml Lysozyme Cellulose Beads 3 - 12 75µ

90EL Affinity, endotoxin removal up to 2000 kDa. 480 µg/ml Cellulose Beads 2 - 13 90µ75ES Affinity, endotoxin removal up to 2 kDa. 185 µg/ml Cellulose Beads 2 - 13 75µ

90C4(butyl)

Hydrophobic interaction for hydrophobic proteins up to4000 kDa

25 mg/ml BSA Cellulose Beads 1 - 13 90µ

90PH(phenyl)

Hydrophobic interaction for hydrophobic proteins and enzymes up to 4000 kDa

30 mg/ml BSA Cellulose Beads 1 - 13 90µ

Cellulose media for biopurification

Page 1 of 1

Cellulose Media for Biopurification



PP-C18T Moderately hydrophobic peptides & oligopeptides.<40 kDa 2% 300Å Spherical

Silica 1.5 - 8 15 & 30µ

PT-C18T Weak hydrophobic peptides/oligopeptides. <50 kDa 2% 200Å Spherical Silica 1.5 - 8 15 & 30µ

PP-C8 Hydrophobic polypeptides & peptides. <80 kDa 2% 300Å Spherical Silica 2 - 7 15 & 30µ

PT-C8 Moderately hydrophobic peptides & oligopeptides.<60 kDa 2% 200Å Spherical

Silica 2 - 7 15 & 30µ

PP-C4 Hydrophobic proteins & polypeptides 50 - 150 kDa 2% 300Å Spherical Silica 2 - 7 15 & 30µ

PT-C4 Hydrophobic peptides & oligopeptides. <60 kDa 2% 200Å Spherical Silica 2 - 7 15 & 30µ

Reverse Phase, for macro-molecules

Page 1 of 1

Reverse Phase for Biopurification


PT-C18T, PP-C18T

O OOH

SiCH

3

CH3

CH3

SiC 18

H37

R R

O OOH

SiCH

3

CH3

CH3

SiC 8H

17

R R

PP-C8, PT-C8

O OOH

SiCH

3

CH3

CH3

SiC 4H

9

R R

PP-C4, PT-C4

22

25

22

25

23

25

24

25

26

24

26

23

26

26

7TLC Optimization

Prior to running a sample on a flash instrument/column it is important to determine the optimum solubility, selectivity and resolution by TLC. The following solvent systems provide a wide range of selectivities and are a useful screening tool. Valuable information is obtained by running the sample in several mobile phases and will improve the odds of obtaining purity using Flash. It is also useful to create a weak and strong polar-ity for each of the mobile phases to obtain additional data points.

The TLC plate which best correlates to puriFlash HC & HP silica columns is part PFT-1000. For additional information, refer to back cover.

Solvent System

Compound Characteristics TLC Conditions Comments

EtOAc:Hexanes

DCM:MeOH + NH3

Diethyl ether: Hexanes or THF

Lipophilic molecules without ionizable groups

Molecules with basic amine functionality

Non-polar molecules

Mixture applied to flash column should be soluble or partially soluble in the mobile phase. Adding DCM will aid solubility but reduce retention.

It is common for streaking to oc-cur with basic amines. To reduce streaking/band broadening add an amine modifier (a few drops of NH3 or a solution of NH3 in MeOH). Adding an amine modifier can improve compound recovery from the column.

Use methyl tetrahydrofuran instead of THF which does not readily form peroxides.

Start at 1:1 and adjust until Rf of target is 0.2 - 0.3

90:10 DCM:MeOH and adjust MeOH until Rf of target is 0.2 - 0.3

Start at 1:1 and adjust until Rf of target is 0.2 - 0.3

DCM:MeOH

DCM:Hexanes: MeOH + NH3

Lipophilic molecules without ionizable groups

Molecules with basic amine functionality

If you have an ester functional group in your product, transesteri-fication is possible. This happens when the product ester is ex-changed with the solvent alcohol used in the mobile phase - if you have an ethyl ester in your product and you use MeOH in the mobile phase you may end up with a small amount of methyl ester in your product. Silica can be acidic and will promote this type of reaction.

If you need to use DCM:MeOH but it is still too polar you can add hexanes (or replace the DCM with hexanes). This will improve the Rf without changing the nature of the column and retain the solubilizing power of the mobile phase.

If above conditions do not move target at 100% EtOAc, add MeOH to DCM at 1% up to 15%.

90:10 DCM:MeOH and adjust Hexanes until Rf of target is 0.2 - 0.3

8

Part number Description Price UnitPF-15SIHCKA PuriFlash Column Kit,15µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $325 KITPF-15SIHCKB PuriFlash Column Kit,15u Si HC, 1 EACH;80g, 120g, 200g, 300g $625 KITPF-15SIHCKC PuriFlash Column Kit,15µ Si HC, 2 EACH; 4G, 12G, 25G, 40G $165 KIT

PF-25SIHCKA PuriFlash Column Kit, 25µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $195 KITPF-25SIHCKB PuriFlash Column Kit, 25µ Si HC, 2 EACH; 80G, 120G, 200G, 300G $675 KITPF-25SIHCKC PuriFlash Column Kit, 25µ Si HC, 1 EACH; 80G, 120G, 200G, 300G $350 KIT

PF-50SIHCKA PuriFlash Column Kit, 50µ Si HC, 4 EACH; 4G, 12G, 25G, 40G $135 KITPF-50SIHCKB PuriFlash Column Kit, 50µ Si HC 2 EACH; 80G, 120G, 200G, 300G $395 KIT

PF-15SIHC/4G PuriFlash Column, 15µ Si HC, 4G $220 20/PKPF-15SIHC/12G PuriFlash Column, 15µ Si HC, 12G $530 20/PKPF-15SIHC/25G PuriFlash Column, 15µ Si HC, 25G $485 12/PKPF-15SIHC/40G PuriFlash Column, 15µ Si HC, 40G $715 12/PKPF-15SIHC/80G PuriFlash Column, 15µ Si HC, 80G $505 4/PKPF-15SIHC/120G PuriFlash Column, 15µ Si HC, 120g $725 4/PKPF-15SIHC/200G PuriFlash Column, 15µ Si HC, 200g $620 2/PKPF-15SIHC/300G PuriFlash Column, 15µ Si HC, 300g $775 2/PKPF-15SIHC/800G PuriFlash Column, 15µ Si HC, 800g $1,225 EAPF-15SIHC/1600G PuriFlash Column, 15µ Si HC, 1600g $2,350 EA

PF-25SIHC/4G PuriFlash Column, 25µ Si HC, 4g $370 40/PKPF-25SIHC/12G PuriFlash Column, 25µ Si HC, 12g $385 30/PKPF-25SIHC/25G PuriFlash Column, 25µ Si HC, 25g $495 25/PKPF-25SIHC/40G PuriFlash Column, 25µ Si HC, 40g $530 20/PKPF-25SIHC/80G PuriFlash Column, 25µ Si HC, 80g $415 10/PKPF-25SIHC/120G PuriFlash Column, 25µ Si HC, 120g $450 8/PKPF-25SIHC/200G PuriFlash Column, 25µ Si HC, 200g $400 4/PKPF-25SIHC/300G PuriFlash Column, 25µ Si HC, 300g $635 4/PKPF-25SIHC/800G PuriFlash Column, 25µ Si HC, 800g $445 EAPF-25SIHC/1600G PuriFlash Column, 25µ Si HC, 1600g $785 EA

PF-50SIHC/4G PuriFlash Column, 50µ Si HC, 4g $275 40/PKPF-50SIHC/12G PuriFlash Column, 50µ Si HC, 12g $305 30/PKPF-50SIHC/25G PuriFlash Column, 50µ Si HC, 25g $320 25/PKPF-50SIHC/40G PuriFlash Column, 50µ Si HC, 40g $320 20/PKPF-50SIHC/80G PuriFlash Column, 50µ Si HC, 80g $335 10/PKPF-50SIHC/120G PuriFlash Column, 50µ Si HC, 120g $370 8/PKPF-50SIHC/200G PuriFlash Column, 50µ Si HC, 200g $310 4/PKPF-50SIHC/300G PuriFlash Column, 50µ Si HC, 300g $425 4/PKPF-50SIHC/800G PuriFlash Column, 50µ Si HC, 800g $260 EAPF-50SIHC/1600G PuriFlash Column, 50µ Si HC, 1600g $530 EAPF-50SIHC-KG PuriFlash 50µ Si HC Bulk $160 KG

Page 1 of 1

PuriFlash Normal Phase HC: High Capacity Silica

9

Part number Description Price UnitPF-15SIHPKA PuriFlash Column Kit, 15µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $475 KITPF-15SIHPKB PuriFlash Column Kit, 15µ Si HP, 1 EACH; 80G, 120G, 200G, 300G $985 KITPF-15SIHPKC PuriFlash Column Kit, 15µ Si HP, 2 EACH; 4G, 12G, 25G, 40G $240 KIT

PF-30SIHPKA PuriFlash Column Kit, 30µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $195 KITPF-30SIHPKB PuriFlash Column Kit, 30µ Si HP, 2 EACH; 80G, 120G, 200G, 300G $675 KITPF-30SIHPKC PuriFlash Column Kit, 30µ Si HP, 1 EACH; 80G, 120G, 200G, 300G $340 KIT

PF-50SIHPKA PuriFlash Column Kit, 50µ Si HP, 4 EACH; 4G, 12G, 25G, 40G $145 KITPF-50SIHPKB PuriFlash Column Kit, 50µ Si HP 2 EACH; 80G, 120G, 200G, 300G $460 KIT

PF-15SIHP/4G PuriFlash Column, 15µ Si HP, 4g $220 20/PKPF-15SIHP/12G PuriFlash Column, 15µ Si HP, 12g $530 20/PKPF-15SIHP/25G PuriFlash Column, 15µ Si HP, 25g $485 12/PKPF-15SIHP/40G PuriFlash Column, 15µ Si HP, 40g $715 12/PKPF-15SIHP/80G PuriFlash Column, 15µ Si HP, 80g $505 4/PKPF-15SIHP/120G PuriFlash Column, 15µ Si HP, 120g $725 4/PKPF-15SIHP/200G PuriFlash Column, 15µ Si HP, 200g $620 2/PKPF-15SIHP/300G PuriFlash Column, 15µ Si HP, 300g $775 2/PKPF-15SIHP/800G PuriFlash Column, 15µ Si HP, 800g $1,225 EAPF-15SIHP/1600G PuriFlash Column, 15µ Si HP, 1600g $2,350 EA

PF-30SIHP/4G PuriFlash Column, 30µ Si HP, 4g $370 40/PKPF-30SIHP/12G PuriFlash Column, 30µ Si HP, 12g $385 30/PKPF-30SIHP/25G PuriFlash Column, 30µ Si HP, 25g $495 25/PKPF-30SIHP/40G PuriFlash Column, 30µ Si HP, 40g $530 20/PKPF-30SIHP/80G PuriFlash Column, 30µ Si HP, 80g $415 10/PKPF-30SIHP/120G PuriFlash Column, 30µ Si HP, 120g $450 8/PKPF-30SIHP/200G PuriFlash Column, 30µ Si HP, 200g $400 4/PKPF-30SIHP/300G PuriFlash Column, 30µ Si HP, 300g $635 4/PKPF-30SIHP/800G PuriFlash Column, 30µ Si HP, 800g $445 EAPF-30SIHP/1600G PuriFlash Column, 30µ Si HP, 1600g $785 EA

PF-50SIHP/4G PuriFlash Column, 50µ Si HP, 4g $265 40/PKPF-50SIHP/12G PuriFlash Column, 50µ Si HP, 12g $295 30/PKPF-50SIHP/25G PuriFlash Column, 50µ Si HP, 25g $310 25/PKPF-50SIHP/40G PuriFlash Column, 50µ Si HP, 40g $310 20/PKPF-50SIHP/80G PuriFlash Column, 50µ Si HP, 80g $325 10/PKPF-50SIHP/120G PuriFlash Column, 50µ Si HP, 120g $360 8/PKPF-50SIHP/200G PuriFlash Column, 50µ Si HP, 200g $275 4/PKPF-50SIHP/300G PuriFlash Column, 50µ Si HP, 300g $415 4/PKPF-50SIHP/800G PuriFlash Column, 50µ Si HP, 800g $250 EAPF-50SIHP/1600G PuriFlash Column, 50µ Si HP, 1600g $390 EA

Page 1 of 1

PuriFlash Normal Phase HP: High Performance Silica

10

Part number Description Price UnitPF-ALB/7G PuriFlash Column, 50µ Al Basic, 7g $60 8/PKPF-ALB/25G PuriFlash Column, 50µ Al Basic, 25g $45 4/PKPF-ALB/40G PuriFlash Column, 50µ Al Basic, 40g $60 4/PKPF-ALB/65G PuriFlash Column, 50µ Al Basic, 65g $80 4/PKPF-ALB/135G PuriFlash Column, 50µ Al Basic, 135g $82 2/PKPF-ALB/200G PuriFlash Column, 50µ Al Basic, 200g $115 2/PKPF-ALB/355G PuriFlash Column, 50µ Al Basic, 355g $183 2/PKPF-ALB/550G PuriFlash Column, 50µ Al Basic, 550g $138 EAPF-ALB/1445G PuriFlash Column, 50µ Al Basic, 1445g $342 EAPF-ALB/2900G PuriFlash Column, 50µ Al Basic, 2900g $568 EA

PF-ALN/7G PuriFlash Column, 50µ Al Neutral, 7g $59 8/PKPF-ALN/25G PuriFlash Column, 50µ Al Neutral, 25g $56 4/PKPF-ALN/40G PuriFlash Column, 50µ Al Neutral, 40g $77 4/PKPF-ALN/65G PuriFlash Column, 50µ Al Neutral, 65g $106 4/PKPF-ALN/135G PuriFlash Column, 50µ Al Neutral, 135g $109 2/PKPF-ALN/200G PuriFlash Column, 50µ Al Neutral, 200g $156 2/PKPF-ALN/355G PuriFlash Column, 50µ Al Neutral, 355g $253 2/PKPF-ALN/550G PuriFlash Column, 50µ Al Neutral, 550g $194 EAPF-ALN/1445G PuriFlash Column, 50µ Al Neutral, 1445g $483 EAPF-ALN/2900G PuriFlash Column, 50µ Al Neutral, 2900g $857 EA

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PuriFlash Normal PhaseBasic & Neutral Alumina

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Part number Description Price UnitPF-15C18XS/6G PuriFlash Column, 15µ C18 XS, 6g $390 4/PKPF-15C18XS/20G PuriFlash Column, 15µ C18 XS, 20g $390 2/PKPF-15C18XS/35G PuriFlash Column, 15µ C18 XS, 35g $225 EAPF-15C18XS/55G PuriFlash Column, 15µ C18 XS, 55g $285 EAPF-15C18XS/120G PuriFlash Column, 15µ C18 XS, 120g $545 EAPF-15C18XS/175G PuriFlash Column, 15µ C18 XS, 175g $736 EAPF-15C18XS/300G PuriFlash Column, 15µ C18 XS, 300g $1,281 EAPF-15C18XS/450G PuriFlash Column, 15µ C18 XS, 450g $1,976 EA

PF-30C18XS/6G PuriFlash Column, 30µ C18 XS, 6g $340 4/PKPF-30C18XS/20G PuriFlash Column, 30µ C18 XS, 20g $340 2/PKPF-30C18XS/35G PuriFlash Column, 30µ C18 XS, 35g $195 EAPF-30C18XS/55G PuriFlash Column, 30µ C18 XS, 55g $240 EAPF-30C18XS/120G PuriFlash Column, 30µ C18 XS, 120g $490 EAPF-30C18XS/175G PuriFlash Column, 30µ C18 XS, 175g $595 EAPF-30C18XS/300G PuriFlash Column, 30µ C18 XS, 300g $1,030 EAPF-30C18XS/450G PuriFlash Column, 30µ C18 XS, 450g $1,595 EAPF-30C18XS/1250G PuriFlash Column, 30µ C18 XS, 1250g $4,275 EAPF-30C18XS/2500G PuriFlash Column, 30µ C18 XS, 2500g $8,495 EA

PuriFlash XS pH Stable C18 Columns, Reverse Phase

11

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Part number Description Price UnitPF-DLE/4G PuriFlash Dryload Column, Empty 7 ml, 12 x 65 mm (FO8880) 80 20/PKPF-DLE/12G PuriFlash Dryload Column, Empty 30 ml, 21 x 80 mm (FO8890) 105 20/PKPF-DLE/25G PuriFlash Dryload Column, Empty 45 ml, 21 x 130 mm (FO8900) 135 20/PKPF-DLE/40G PuriFlash Dryload Column, Empty 75 ml, 27 x 130 mm (FL9521) 165 20/PKPF-DLE/80G PuriFlash Dryload Column, Empty 145 ml, 31 x 193 mm 95 5/PKPF-DLE/120G PuriFlash Dryload Column, Empty 215 ml, 36 x 212 mm 100 5/PKPF-DLE/200G PuriFlash Dryload Column, Empty 375 ml, 60 x 133 mm 125 5/PKPF-DLE/300G PuriFlash Dryload Column, Empty 575 ml, 60 x 205 mm 185 5/PKPF-DLE/KA PuriFlash Dryload Column Kit, Empty, 2 Each; 4g, 12g, 25g, 40g 35 KIT

PF-DLSIHC08/4G PuriFlash Dryload Column, HC Silica 3g, 7 ml, 12 x 65 mm 95 20/PKPF-DLSIHC08/12G PuriFlash Dryload Column, HC Silica 11g, 30 ml, 21 x 80 mm 140 20/PKPF-DLSIHC08/25G PuriFlash Dryload Column, HC Silica 20g, 45 ml, 21 x 130mm 157 20/PKPF-DLSIHC08/40G PuriFlash Dryload Column, HC Silica 32g, 75 ml, 27 x 130 mm 189 20/PKPF-DLSIHC08/K PuriFlash Dryload Column Kit, HC Silica 4 each; 3g,11g, 20g,30g 125 KIT

PF-DLCET08/12G PuriFlash Dryload Column, Celite 5g, 30 ml, 21 x 80 mm 140 20/PKPF-DLCET08/25G PuriFlash Dryload Column, Celite 10g, 45 ml, 21 x 130mm 180 20/PKPF-DLCET08/40G PuriFlash Dryload Column, Celite 15g, 75 ml, 27 x 130 mm 210 20/PKPF-DLCET08/4G PuriFlash Dryload Column, Celite 2g, 7 ml, 12 x 65 mm 95 20/PK

PF-DLP/4G Top Frit Plunger for 12 mm ID columns 10 EAPF-DLP/25G Top Frit Plunger for 21 mm ID columns (12 & 25 g) 10 EAPF-DLP/40G Top Frit Plunger for 27 mm ID columns 10 EAPF-DLP/80G Top Frit Plunger for 31 mm ID columns 20 EAPF-DLP/120G Top Frit Plunger for 36 mm ID columns 25 EAPF-DLP/200G Top Frit Plunger for 60 mm ID columns (200 & 300 g) 30 EA

PuriFlash Dry Load ColumnsEmpty or Partially Filled with Silica or Celite

• Self contained, no adapters or special fittings• Hand tight to 15 bar / 215 PSI• Compatible with all Flash systems

Based on a proven design, the PuriFlash Dry Load columns are simple to load and the luer fittings provide a universal fit for all flash systems.

The empty PuriFlash Dry Load columns allow you to add your own silica or celite. The HC Series is supplied with high capacity silica for maximum adsorption. The Celite Series is supplied with celite and recommended for non-adsorptive loading.

12

Part number Description Price UnitPF-30DIOL/6G PuriFlash Column, 30µ Diol, 6g $290 4/PKPF-30DIOL/20G PuriFlash Column, 30µ Diol, 20g $285 2/PKPF-30DIOL/35G PuriFlash Column, 30µ Diol, 35g $160 EAPF-30DIOL/55G PuriFlash Column, 30µ Diol, 55g $200 EAPF-30DIOL/120G PuriFlash Column, 30µ Diol, 120g $390 EAPF-30DIOL/175G PuriFlash Column, 30µ Diol, 175g $575 EAPF-30DIOL/300G PuriFlash Column, 30µ Diol, 300g $1,075 EAPF-30DIOL/450G PuriFlash Column, 30µ Diol, 450g $1,655 EAPF-30DIOL/1250G PuriFlash Column, 30µ Diol, 1250g $3,410 EAPF-30DIOL/2500G PuriFlash Column, 30µ Diol, 2500g $6,825 EA

PF-50DIOL/6G PuriFlash Column, 50µ Diol, 6g $75 4/PKPF-50DIOL/20G PuriFlash Column, 50µ Diol, 20g $109 2/PKPF-50DIOL/35G PuriFlash Column, 50µ Diol, 35g $85 EAPF-50DIOL/55G PuriFlash Column, 50µ Diol, 55g $130 EAPF-50DIOL/120G PuriFlash Column, 50µ Diol, 120g $277 EAPF-50DIOL/175G PuriFlash Column, 50µ Diol, 175g $406 EAPF-50DIOL/300G PuriFlash Column, 50µ Diol, 300g $701 EAPF-50DIOL/450G PuriFlash Column, 50µ Diol, 450g $1,081 EAPF-50DIOL/1250G PuriFlash Column, 50µ Diol, 1250g $2,818 EAPF-50DIOL/2500G PuriFlash Column, 50µ Diol, 2500g $5,537 EA

PF-30HILIC/6G PuriFlash Column, 30µ Hilic, 6g $78 4/PKPF-30HILIC/20G PuriFlash Column, 30µ Hilic, 20g $122 2/PKPF-30HILIC/35G PuriFlash Column, 30µ Hilic, 35g $95 EAPF-30HILIC/55G PuriFlash Column, 30µ Hilic, 55g $149 EAPF-30HILIC/120G PuriFlash Column, 30µ Hilic, 120g $319 EAPF-30HILIC/175G PuriFlash Column, 30µ Hilic, 175g $467 EAPF-30HILIC/300G PuriFlash Column, 30µ Hilic, 300g $813 EAPF-30HILIC/450G PuriFlash Column, 30µ Hilic, 450g $1,250 EAPF-30HILIC/1250G PuriFlash Column, 30µ Hilic, 1250g $3,289 EAPF-30HILIC/2500G PuriFlash Column, 30µ Hilic, 2500g $6,488 EA

PF-50CN/6G PuriFlash Column, 50µ CN 6G $89 4PKPF-50CN/20G PuriFlash Column, 50µ CN 20G $138 2PKPF-50CN/35G PuriFlash Column, 50µ CN 35G $111 EAPF-50CN/55G PuriFlash Column, 50µ CN 55G $173 EAPF-50CN/120G PuriFlash Column, 50µ CN 120G $370 EAPF-50CN/175G PuriFlash Column, 50µ CN 175G $543 EAPF-50CN/300G PuriFlash Column, 50µ CN 300G $945 EAPF-50CN/450G PuriFlash Column, 50µ CN, 450g $1,455 EAPF-50CN/1250G PuriFlash Column, 50µ CN, 1250g $3,823 EAPF-50CN/2500G PuriFlash Column, 50µ CN, 2500g $7,563 EA

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PuriFlash Normal PhaseDiol, HILIC & CN Bonded Silica

13

Affordable chiral purification is now a reality. SureFlash columns are packed with a variety of chiral stationary phases which match those used in analytical development.

SureFlash columns are stable up to 80 bar/1160 PSI and use axial compression to compensate for voids at the inlet which may develop over time.

The analytical method was scaled up from a 250 x 4.6 mm to a 250 x 15 mm column.

100% purity was achieved on sample loads of 40 mg and 120 mg. The column was run on the puriFlash 430 at an average pressure of 7 bar.

Based on the scaling factors a 250 x 50 mm column would be suitable for sample loads in excess of 1 gram.

Column Size Scaling Factor Sample Load250 x 4.6 mm 1 <5 ug

250 x 10 mm 5 53 mg

250 x 15 mm 11 120 mg

250 x 25 mm 30 333 mg

250 x 50 mm 118 1333 mg

Test ConditionsSample: WarfarinChiral Phase: Chiralpak ADMobile Phase: 80:20 Heptane:Ethanol + 0.2% TFA Analytical: 250 x 4.6 mm, 10 um particle size 1.0 ml/min, 450 PSIPreparative: 250 x 15 mm, 20 um particle size 11.0 ml/min 7 Bar / 100 PSI

SureFlash Chiral Purification

High Pressure Axial Compression Column Hardware.

Eliminate voids by rotating the end

fitting.

Phase Price $ Price $ Price $ Price $250 x 10 mm

80 bar/1160 PSI250 x 15 mm

70 bar/1015 PSI250 x 25 mm50 bar/715 PSI

250 x 50 mm30 bar/435 PSI

Chiralpak AD 20 µm SF10101 2900 SF20101 3600 SF30101 5900 SF40101 Quote

Chiralpak OD 20 µm SF10102 2900 SF20102 3600 SF30102 5900 SF40102 Quote

Chiralpak IA 20 µm SF10103 3200 SF20103 3900 SF30103 6200 SF40103 Quote

Chiralpak IC 20 µm SF10104 3200 SF20104 3900 SF30104 6200 SF40104 Quote

40mg

120mg

Analytical

Warfarin

14

Interchim Prep columns redefine the boundaries for Prep HPLC by maximizing throughput at a reduced cost. 100% purity is routinely achieved using columns packed with 10µ and 15µ particles, which are considerably less expensive than 5µ particles.

A wide range of bonded phases provide the selectivity in all situations to ensure optimum resolution. Whether you require loading in 100% aqueous, extended pH stability or high sample loading, Interchim has an affordable prep column.

Uptisphere Strategy Bonded Phase Specifications

Uptisphere Strategy Prep Column Ordering Information

Pre-column filters are an inexpensive alternative to guard columns and protect the prep column from the most common cause of reduced lifetime: sample precipitation.

puriFlash Pre-column Filter

Approximate purification capacity relative to column diameter.

ID Sample Load 250 10 mm 0.5 - 10 mg21.2 mm 2 - 50 mg30 mm 50 - 500 mg50 mm 100mg - 1g

Best Value

0.3

0.5

3.0

A significant function of silica cost is the particle size distribution (dp–Particle Diameter). The tighter the distribution the higher the cost.

The 150 x 30.0mm column is the most popular column geometry. The following lengths and IDs are also available. ID: 21.2 mm, 50.0 mm, 60.0 mm, 75.0 mm. Length: 50 mm, 100 mm, 250 mm, 275 mm. Please visit www.interchiminc.com for complete ordering information.

Phase Endcapping %CBase Silica Pore Size Surface Area pH Range Application

C18-2 Ultra-Pure 100Å 425 m2/g Multi-Step 19% 1 - 10 General purpose for mid to non-polar compounds.

C18HQ High Quality 100Å 425 m2/g Multi-Step 19% 1.5 - 8 Lower cost alternative to Ultra-

Pure silica

C18RP Ultra-Pure 100Å 425 m2/g Mixed-Mode 16% 1.5 - 8 Stable in 100% aqueous. Mid & non polar compounds

C18NEC Ultra-Pure 120Å 320 m2/g None 16% 1.5 - 6.5 Greater retention for polar and mid-polar compounds

Preparative HPLC Columns

Particle C18-2 Price C18HQ Price C18RP Price C18NEC Price5µ US5C182-150/300 425010µ PF10C18HQ-150/300 3550 US10RP-150/300 3550 UP10NEC-150/300 355015µ PF15C18HQ-150/300 2950 US15RP-150/300 2950 UP15NEC-150/300 2950

Part No. PuriFlash Pre-Column Filter Unit PriceCE4600 Prep Pre-column / Inline Filter Holder, (Includes 2µ Filter) EA 318CE4620 Prep Pre-column / Inline Filter, 2.0µ Stainless Steel Filter EA 21CE4630FC Prep Pre-column / Inline Filter, O-Rings, Fluorocarbon EA 22

15

0.0

75.0

-75.0

150.0

300.0

375.0

450.0

525.0

600.0

675.0

225.0

0.00 cv 0.75 cv 1.50 cv 2.25 cv 3.00 cv

The puriFlash 450, 4100 and 4250 can be used as a traditional flash instrument or as a preparative HPLC. Similar to a preparative HPLC, the sample is loaded into the loop of the injection valve. A 2ml loop is standard with options up to 10ml.

400mg IntermediateIn DMSO GradientA. Water + 0.1% TFAB. Acetonitrile + 0.1% TFA 0-1 Minute, 10% B12 Minutes, 70% B14 Minutes, 70% B16 Minutes, 100% B40 ml/min.UV @ 254 & 280nm

1.2 g Sample Load2ml Injection, 150mg/ml, Caffeine, Sulfadimethoxine, acetophenome, ketoprofen in DMSOGradientA. Water + 3% n-PrOHB. Acetonitrile + 3% n-PrOH 0-30 Seconds, 10% B10 Minutes, 90% B40 ml/min.UV @ 254 & 280nm

180mg AmideGradientA. 2mM NH3 in WaterB. 2mM NH3 in Acetonitrile 0-3 Minutes, 60% B20 Minutes, 85% B25 ml/min.UV @ 254 & 280nm

0.0

200.0

400.0

600.0

-200.0

1000.0

800.0

1400.0

1600.0

1800.0

1200.0

00:00 02:00

mAU

04:00 06:00 08:00 10:00

0.0

500.0

-500.0

1000.0

2000.0

2500.0

3000.0

1500.0

0.00 cv 0.75 cv 1.50 cv 2.25 cv 3.00 cv 3.75 cv 4.50 cv 5.25 cv 6.00 cv

mAU

Reverse phase purification on the puriFlash 450 using a preparative HPLC column. PuriFlash C18-HQ 10µ, 30 x 150mm. (Part number PF10C18HQ-150/300)

Preparative HPLC Columns

16

Reverse phase, Scale-up from analytical to Flash

Normal Phase, Isomeric Separation

150mg crude on a 35g 15µ C18

1 gram load on a 40g 15µ SI HC puriFlash® column

puriFlash 15µ C18HP 35g, Part number PF-15C18HP/35G

SolventA: Hexane, B: Ethyl acetate, Flow Rate: 30 ml/min, UV 254 nmGradientCV % A % B0 100 05 80 2010 70 30 14.25 40 6015 5 95

Due to the complex nature of the crude mixture, a dry load column was used to introduce the sample on the puriFlash 450.

Part Number: PF-15SIHC/40G 15µ SI HC puriFlash column, 40g

Part Number: PF-DLE/4G puriFlash Dry-Load column, 4g

RfA = 0.3

A B I

RfB = 0.2

RfI = 0.15

∆CVB-A = 1.7

A B I

0.0

50.0

-50.0

100.0

200.0

250.0

300.0

350.0

150.0

10

20

00

30

50

60

70

80

90

100%

40

05:00 10:00 15:00 20:00 25:00 30:00 35:00

Compound of interest

40:00

Compound of interest

Analytical, 250 x 4.6mm 5µ C18Compound of interest

SolventA: WaterB: AcetonitrileC: Ammonium Formate - 10% in 50/50 Acetonitrile/WaterFlow Rate: Analytical 1.0 ml/mn , Flash 20 ml/minUV 254 nm

17

Part number Description Price UnitPF-30NH2/6G PuriFlash Column, 30µ NH2, 6g $280 4/PKPF-30NH2/20G PuriFlash Column, 30µ NH2, 20g $275 2/PKPF-30NH2/35G PuriFlash Column, 30µ NH2, 35g $165 EAPF-30NH2/55G PuriFlash Column, 30µ NH2, 55g $195 EAPF-30NH2/120G PuriFlash Column, 30µ NH2, 120g $409 EAPF-30NH2/175G PuriFlash Column, 30µ NH2, 175g $598 EAPF-30NH2/300G PuriFlash Column, 30µ NH2, 300g $1,037 EAPF-30NH2/450G PuriFlash Column, 30µ NH2, 450g $1,602 EAPF-30NH2/1250G PuriFlash Column, 30µ NH2, 1250g $4,185 EAPF-30NH2/2500G PuriFlash Column, 30µ NH2, 2500g $8,281 EA

PF-50NH2/6G PuriFlash Column, 50µ NH2, 6g $95 4/PKPF-50NH2/20G PuriFlash Column, 50µ NH2, 20g $125 2/PKPF-50NH2/35G PuriFlash Column, 50µ NH2, 35g $100 EAPF-50NH2/55G PuriFlash Column, 50µ NH2, 55g $150 EAPF-50NH2/120G PuriFlash Column, 50µ NH2, 120g $320 EAPF-50NH2/175G PuriFlash Column, 50µ NH2, 175g $455 EAPF-50NH2/300G PuriFlash Column, 50µ NH2, 300g $795 EAPF-50NH2/450G PuriFlash Column, 50µ NH2, 450g $1,200 EAPF-50NH2/1250G PuriFlash Column, 50µ NH2, 1250g $3,050 EAPF-50NH2/2500G PuriFlash Column, 50µ NH2, 2500g $6,500 EA

PF-50NH2HC/6G PuriFlash Column, 50µ NH2 HC, 6g $43 4/PKPF-50NH2HC/20G PuriFlash Column, 50µ NH2 HC, 20g $57 2/PKPF-50NH2HC/35G PuriFlash Column, 50µ NH2 HC, 35g $43 EAPF-50NH2HC/55G PuriFlash Column, 50µ NH2 HC, 55g $68 EAPF-50NH2HC/120G PuriFlash Column, 50µ NH2 HC, 120g $146 EAPF-50NH2HC/175G PuriFlash Column, 50µ NH2 HC, 175g $211 EAPF-50NH2HC/300G PuriFlash Column, 50µ NH2 HC, 300g $362 EAPF-50NH2HC/450G PuriFlash Column, 50µ NH2 HC, 450g $556 EAPF-50NH2HC/1250G PuriFlash Column, 50µ NH2 HC, 1250g $1,461 EAPF-50NH2HC/2500G PuriFlash Column, 50µ NH2 HC, 2500g $2,822 EA

PF-50DEAP/6G PuriFlash Column, 50µ DEAP, 6g $138 6/PKPF-50DEAP/20G PuriFlash Column, 50µ DEAP, 20g $415 6/PKPF-50DEAP/35G PuriFlash Column, 50µ DEAP, 35g $651 6/PKPF-50DEAP/55G PuriFlash Column, 50µ DEAP, 55g $168 EAPF-50DEAP/120G PuriFlash Column, 50µ DEAP, 120g $362 EAPF-50DEAP/175G PuriFlash Column, 50µ DEAP, 175g $527 EAPF-50DEAP/300G PuriFlash Column, 50µ DEAP, 300g $913 EAPF-50DEAP/450G PuriFlash Column, 50µ DEAP, 450g $1,411 EAPF-50DEAP/1250G PuriFlash Column, 50µ DEAP, 1250g $3,684 EAPF-50DEAP/2500G PuriFlash Column, 50µ DEAP, 2500g $7,274 EA

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PuriFlash Normal PhaseNH2 & DEAP Bonded Silica

18 PuriFlash Ion ExchangeSCX & SAX Bonded Silica

PuriFlash Ion ExchangeMM1 Bonded Silica

PuriFlash Reverse PhaseP6 for Natural Products

Part number Description Price UnitPF-50SCX/6G PuriFlash Column, 50µ SCX, 6g $165 4/PKPF-50SCX/20G PuriFlash Column, 50µ SCX, 20g $268 2/PKPF-50SCX/35G PuriFlash Column, 50µ SCX, 35g $212 EAPF-50SCX/55G PuriFlash Column, 50µ SCX, 55g $336 EAPF-50SCX/120G PuriFlash Column, 50µ SCX, 120g $719 EAPF-50SCX/175G PuriFlash Column, 50µ SCX, 175g $1,051 EAPF-50SCX/300G PuriFlash Column, 50µ SCX, 300g $1,832 EAPF-50SCX/450G PuriFlash Column, 50µ SCX, 450g $2,830 EAPF-50SCX/1250G PuriFlash Column, 50µ SCX, 1250g $7,416 EAPF-50SCX/2500G PuriFlash Column, 50µ SCX, 2500g ###### EA

PF-50MM1/6G PuriFlash Column, 50µ MM1 (RP/SCX), 6g $95 4/PKPF-50MM1/20G PuriFlash Column, 50µ MM1 (RP/SCX), 20g $144 2/PKPF-50MM1/35G PuriFlash Column, 50µ MM1 (RP/SCX), 35g $115 EAPF-50MM1/55G PuriFlash Column, 50µ MM1 (RP/SCX), 55g $177 EAPF-50MM1/120G PuriFlash Column, 50µ MM1 (RP/SCX), 120g $377 EAPF-50MM1/175G PuriFlash Column, 50µ MM1 (RP/SCX), 175g $551 EAPF-50MM1/300G PuriFlash Column, 50µ MM1 (RP/SCX), 300g $954 EAPF-50MM1/450G PuriFlash Column, 50µ MM1 (RP/SCX), 450g $1,475 EAPF-50MM1/1250G PuriFlash Column, 50µ MM1 (RP/SCX), 1250g $3,849 EAPF-50MM1/2500G PuriFlash Column, 50µ MM1 (RP/SCX), 2500g $7,610 EA

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Part number Description Price UnitPF-50SAX/6G PuriFlash Column, 50µ SAX, 6g $275 4/PKPF-50SAX/20G PuriFlash Column, 50µ SAX, 20g $375 2/PKPF-50SAX/35G PuriFlash Column, 50µ SAX, 35g $355 EAPF-50SAX/55G PuriFlash Column, 50µ SAX, 55g $540 EAPF-50SAX/120G PuriFlash Column, 50µ SAX, 120g $865 EAPF-50SAX/175G PuriFlash Column, 50µ SAX, 175g $1,075 EAPF-50SAX/300G PuriFlash Column, 50µ SAX, 300g $1,905 EAPF-50SAX/450G PuriFlash Column, 50µ SAX, 450g $3,060 EAPF-50SAX/1250G PuriFlash Column, 50µ SAX, 1250g $8,260 EAPF-50SAX/2500G PuriFlash Column, 50µ SAX, 2500g $16,450 EA

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PF-100P6/2G PuriFlash Column, 100µ P6 Polyamide, 2g $30 4/PKPF-100P6/8G PuriFlash Column, 100µ P6 Polyamide, 8g $40 2/PKPF-100P6/12G PuriFlash Column, 100µ P6 Polyamide, 12g $60 2/PKPF-100P6/20G PuriFlash Column, 100µ P6 Polyamide, 20g $85 2/PKPF-100P6/45G PuriFlash Column, 100µ P6 Polyamide, 45g $88 EAPF-100P6/65G PuriFlash Column, 100µ P6 Polyamide, 65g $124 EAPF-100P6/115G PuriFlash Column, 100µ P6 Polyamide, 115g $209 EAPF-100P6/175G PuriFlash Column, 100µ P6 Polyamide, 175g $321 EAPF-100P6/465G PuriFlash Column, 100µ P6 Polyamide, 465g $819 EAPF-100P6/940G PuriFlash Column, 100µ P6 Polyamide, 940g $1,531 EA

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Quote

19

Part number Description Price UnitPF-15C18HP/6G PuriFlash Column, 15µ C18 HP, 6g $390 4/PKPF-15C18HP/20G PuriFlash Column, 15µ C18 HP, 20g $390 2/PKPF-15C18HP/35G PuriFlash Column, 15µ C18 HP, 35g $225 EAPF-15C18HP/55G PuriFlash Column, 15µ C18 HP, 55g $285 EAPF-15C18HP/120G PuriFlash Column, 15µ C18 HP, 120g $545 EAPF-15C18HP/175G PuriFlash Column, 15µ C18 HP, 175g $736 EAPF-15C18HP/300G PuriFlash Column, 15µ C18 HP, 300g $1,281 EAPF-15C18HP/450G PuriFlash Column, 15µ C18 HP, 450g $1,976 EAPF-15C18HP/1250G PuriFlash Column, 15µ C18 HP, 1250g $5,168 EAPF-15C18HP/2500G PuriFlash Column, 15µ C18 HP, 2500g $10,252 EA

PF-30C18HP/6G PuriFlash Column, 30µ C18 HP, 6g $340 4/PKPF-30C18HP/20G PuriFlash Column, 30µ C18 HP, 20g $340 2/PKPF-30C18HP/35G PuriFlash Column, 30µ C18 HP, 35g $195 EAPF-30C18HP/55G PuriFlash Column, 30µ C18 HP, 55g $240 EAPF-30C18HP/120G PuriFlash Column, 30µ C18 HP, 120g $490 EAPF-30C18HP/175G PuriFlash Column, 30µ C18 HP, 175g $540 EAPF-30C18HP/300G PuriFlash Column, 30µ C18 HP, 300g $990 EAPF-30C18HP/450G PuriFlash Column, 30µ C18 HP, 450g $1,490 EAPF-30C18HP/1250G PuriFlash Column, 30µ C18 HP, 1250g $3,445 EAPF-30C18HP/2500G PuriFlash Column, 30µ C18 HP, 2500g $6,995 EA


PF-15C18HQ/6G PuriFlash Column, 15µ C18 HQ, 6g $400 4/PKPF-15C18HQ/20G PuriFlash Column, 15µ C18 HQ, 20g $400 2/PKPF-15C18HQ/35G PuriFlash Column, 15µ C18 HQ, 35g $235 EAPF-15C18HQ/55G PuriFlash Column, 15µ C18 HQ, 55g $295 EAPF-15C18HQ/120G PuriFlash Column, 15µ C18 HQ, 120g $595 EAPF-15C18HQ/175G PuriFlash Column, 15µ C18 HQ, 175g $855 EAPF-15C18HQ/300G PuriFlash Column, 15µ C18 HQ, 300g $1,482 EAPF-15C18HQ/450G PuriFlash Column, 15µ C18 HQ, 450g $2,282 EAPF-15C18HQ/1250G PuriFlash Column, 15µ C18 HQ, 1250g $6,003 EAPF-15C18HQ/2500G PuriFlash Column, 15µ C18 HQ, 2500g $11,925 EA

PF-15C18HC/6G PuriFlash Column, 15µ C18 HC, 6g $390 4/PK

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PuriFlash Reverse PhaseC18HP & C18HQ Bonded Silica

20

Part number Description Price UnitPF-15C18HP/6G PuriFlash Column, 15µ C18 HP, 6g $390 4/PKPF-15C18HP/20G PuriFlash Column, 15µ C18 HP, 20g $390 2/PKPF-15C18HP/35G PuriFlash Column, 15µ C18 HP, 35g $225 EAPF-15C18HP/55G PuriFlash Column, 15µ C18 HP, 55g $285 EAPF-15C18HP/120G PuriFlash Column, 15µ C18 HP, 120g $545 EAPF-15C18HP/175G PuriFlash Column, 15µ C18 HP, 175g $736 EAPF-15C18HP/300G PuriFlash Column, 15µ C18 HP, 300g $1,281 EAPF-15C18HP/450G PuriFlash Column, 15µ C18 HP, 450g $1,976 EAPF-15C18HP/1250G PuriFlash Column, 15µ C18 HP, 1250g $5,168 EAPF-15C18HP/2500G PuriFlash Column, 15µ C18 HP, 2500g $10,252 EA



PF-15C18HQ/6G PuriFlash Column, 15µ C18 HQ, 6g $400 4/PKPF-15C18HQ/20G PuriFlash Column, 15µ C18 HQ, 20g $400 2/PKPF-15C18HQ/35G PuriFlash Column, 15µ C18 HQ, 35g $235 EAPF-15C18HQ/55G PuriFlash Column, 15µ C18 HQ, 55g $295 EAPF-15C18HQ/120G PuriFlash Column, 15µ C18 HQ, 120g $595 EAPF-15C18HQ/175G PuriFlash Column, 15µ C18 HQ, 175g $855 EAPF-15C18HQ/300G PuriFlash Column, 15µ C18 HQ, 300g $1,482 EAPF-15C18HQ/450G PuriFlash Column, 15µ C18 HQ, 450g $2,282 EAPF-15C18HQ/1250G PuriFlash Column, 15µ C18 HQ, 1250g $6,003 EAPF-15C18HQ/2500G PuriFlash Column, 15µ C18 HQ, 2500g $11,925 EA

PF-15C18HC/6G PuriFlash Column, 15µ C18 HC, 6g $390 4/PK

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PuriFlash Reverse PhaseC18HC Bonded Silica

PF-15C18HC/6G PuriFlash Column, 15µ C18 HC, 6g $390 4/PKPF-15C18HC/20G PuriFlash Column, 15µ C18 HC, 20g $390 2/PKPF-15C18HC/35G PuriFlash Column, 15µ C18 HC, 35g $225 EAPF-15C18HC/55G PuriFlash Column, 15µ C18 HC, 55g $285 EAPF-15C18HC/120G PuriFlash Column, 15µ C18 HC, 120g $545 EAPF-15C18HC/175G PuriFlash Column, 15µ C18 HC, 175g $736 EAPF-15C18HC/300G PuriFlash Column, 15µ C18 HC, 300g $1,281 EAPF-15C18HC/450G PuriFlash Column, 15µ C18 HC, 450g $1,976 EAPF-15C18HC/1250G PuriFlash Column, 15µ C18 HC, 1250g $5,168 EAPF-15C18HC/2500G PuriFlash Column, 15µ C18 HC, 2500g $10,252 EA

PF-30C18HC/6G PuriFlash Column, 30µ C18 HC, 6g $340 4/PKPF-30C18HC/20G PuriFlash Column, 30µ C18 HC, 20g $340 2/PKPF-30C18HC/35G PuriFlash Column, 30µ C18 HC, 35g $195 EAPF-30C18HC/55G PuriFlash Column, 30µ C18 HC, 55g $240 EAPF-30C18HC/120G PuriFlash Column, 30µ C18 HC, 120g $490 EAPF-30C18HC/175G PuriFlash Column, 30µ C18 HC, 175g $540 EAPF-30C18HC/300G PuriFlash Column, 30µ C18 HC, 300g $990 EAPF-30C18HC/450G PuriFlash Column, 30µ C18 HC, 450g $1,490 EAPF-30C18HC/1250G PuriFlash Column, 30µ C18 HC, 1250g $3,445 EAPF-30C18HC/2500G PuriFlash Column, 30µ C18 HC, 2500g $6,995 EA

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PuriFlash Reverse PhaseHigh Capacity Polymers

Part number Description Price UnitPF-X/2G PuriFlash Column, Atoll X 40µ 2G $103 4/PKPF-X/7G PuriFlash Column, Atoll X 40µ 7G $159 2/PKPF-X/10G PuriFlash Column, Atoll X 40µ 10G $124 EAPF-X/18G PuriFlash Column, Atoll X 40µ 18G $186 EAPF-X/40G PuriFlash Column, Atoll X 40µ 40G $421 EAPF-X/55G PuriFlash Column, Atoll X 40µ 55G $608 EAPF-X/100G PuriFlash Column, Atoll X 40µ 100G $1,061 EAPF-X/150G PuriFlash Column, Atoll X 40µ 150G $1,628 EAPF-X/400G PuriFlash Column, Atoll X 40µ 400G $4,282 EAPF-X/800G PuriFlash Column, Atoll X 40µ 800G $8,492 EA

PF-302H/2G PuriFlash Column, 302H 30µ 2G $143 4/PKPF-302H/7G PuriFlash Column, 302H 30µ 7G $232 2/PKPF-302H/10G PuriFlash Column, 302H 30µ 10G $181 EAPF-302H/18G PuriFlash Column, 302H 30µ 18G $278 EAPF-302H/40G PuriFlash Column, 302H 30µ 40G $621 EAPF-302H/55G PuriFlash Column, 302H 30µ 55G $896 EAPF-302H/100G PuriFlash Column, 302H 30µ 100G $1,577 EAPF-302H/150G PuriFlash Column, 302H 30µ 150G $2,417 EAPF-302H/400G PuriFlash Column, 302H 30µ 400G $6,359 EAPF-302H/800G PuriFlash Column, 302H 30µ 800G ###### EA

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Quote

21

Part number Description Price UnitPF-15C18AQ/6G PuriFlash Column, 15µ C18AQ, 6g $390 4/PKPF-15C18AQ/20G PuriFlash Column, 15µ C18AQ, 20g $390 2/PKPF-15C18AQ/35G PuriFlash Column, 15µ C18AQ, 35g $225 EAPF-15C18AQ/55G PuriFlash Column, 15µ C18AQ, 55g $285 EAPF-15C18AQ/120G PuriFlash Column, 15µ C18AQ, 120g $545 EAPF-15C18AQ/175G PuriFlash Column, 15µ C18AQ, 175g $736 EA

PuriFlash Column, 15µ C18AQ, 300g $1,281 EAPF-15C18AQ/450G PuriFlash Column, 15µ C18AQ, 450g $1,976 EAPF-15C18AQ/1250G PuriFlash Column, 15µ C18AQ, 1250g $5,168 EAPF-15C18AQ/2500G PuriFlash Column, 15µ C18AQ, 2500g $10,252 EA

PF-30C18AQ/6G PuriFlash Column, 30µ C18AQ, 6g $340 4/PKPF-30C18AQ/20G PuriFlash Column, 30µ C18AQ, 20g $340 2/PKPF-30C18AQ/35G PuriFlash Column, 30µ C18AQ, 35g $195 EAPF-30C18AQ/55G PuriFlash Column, 30µ C18AQ, 55g $240 EAPF-30C18AQ/120G PuriFlash Column, 30µ C18AQ, 120g $490 EAPF-30C18AQ/175G PuriFlash Column, 30µ C18AQ, 175g $540 EAPF-30C18AQ/300G PuriFlash Column, 30µ C18AQ, 300g $990 EAPF-30C18AQ/450G PuriFlash Column, 30µ C18AQ, 450g $1,490 EAPF-30C18AQ/1250G PuriFlash Column, 30µ C18AQ, 1250g $3,445 EAPF-30C18AQ/2500G PuriFlash Column, 30µ C18AQ, 2500g $6,995 EA

PF-15RPAQ/6G PuriFlash Column, 15µ RPAQ, 6g $390 4/PKPF-15RPAQ/20G PuriFlash Column, 15µ RPAQ, 20g $390 2/PKPF-15RPAQ/35G PuriFlash Column, 15µ RPAQ, 35g $225 EAPF-15RPAQ/55G PuriFlash Column, 15µ RPAQ, 55g $285 EAPF-15RPAQ/120G PuriFlash Column, 15µ RPAQ, 120g $545 EAPF-15RPAQ/175G PuriFlash Column, 15µ RPAQ, 175g $736 EAPF-15RPAQ/300G PuriFlash Column, 15µ RPAQ, 300g $1,281 EAPF-15RPAQ/450G PuriFlash Column, 15µ RPAQ, 450g $1,976 EAPF-15RPAQ/1250G PuriFlash Column, 15µ RPAQ, 1250g $5,168 EAPF-15RPAQ/2500G PuriFlash Column, 15µ RPAQ, 2500g $10,252 EA

PF-30RPAQ/6G PuriFlash Column, 30µ RPAQ, 6g $340 4/PKPF-30RPAQ/20G PuriFlash Column, 30µ RPAQ, 20g $340 2/PKPF-30RPAQ/35G PuriFlash Column, 30µ RPAQ, 35g $195 EAPF-30RPAQ/55G PuriFlash Column, 30µ RPAQ, 55g $240 EAPF-30RPAQ/120G PuriFlash Column, 30µ RPAQ, 120g $490 EAPF-30RPAQ/175G PuriFlash Column, 30µ RPAQ, 175g $540 EAPF-30RPAQ/300G PuriFlash Column, 30µ RPAQ, 300g $990 EAPF-30RPAQ/450G PuriFlash Column, 30µ RPAQ, 450g $1,490 EAPF-30RPAQ/1250G PuriFlash Column, 30µ RPAQ, 1250g $3,445 EAPF-30RPAQ/2500G PuriFlash Column, 30µ RPAQ, 2500g $6,995 EA

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PuriFlash Reverse PhaseHigh Aqueous C18 Bonded Silica

PF-15C18AQ/300G

22

Part number Description Price UnitPP-15C18T/6G PuriFlash Column, 15µ PP 300Å C18T, 6g $210 2/PKPP-15C18T/20G PuriFlash Column, 15µ PP 300Å C18T, 20g $210 EAPP-15C18T/35G PuriFlash Column, 15µ PP 300Å C18T, 35g $345 EAPP-15C18T/55G PuriFlash Column, 15µ PP 300Å C18T, 55g $545 EAPP-15C18T/120G PuriFlash Column, 15µ PP 300Å C18T, 120g $1,166 EAPP-15C18T/175G PuriFlash Column, 15µ PP 300Å C18T, 175g $1,708 EAPP-15C18T/300G PuriFlash Column, 15µ PP 300Å C18T, 300g $2,989 EAPP-15C18T/450G PuriFlash Column, 15µ PP 300Å C18T, 450g $4,618 EAPP-15C18T/1250G PuriFlash Column, 15µ PP 300Å C18T, 1250g $12,113 EAPP-15C18T/2500G PuriFlash Column, 15µ PP 300Å C18T, 2500g $24,178 EA

PP-30C18T/6G PuriFlash Column, 30µ PP 300Å C18T, 6g $170 2/PKPP-30C18T/20G PuriFlash Column, 30µ PP 300Å C18T, 20g $170 EAPP-30C18T/35G PuriFlash Column, 30µ PP 300Å C18T, 35g $205 EAPP-30C18T/55G PuriFlash Column, 30µ PP 300Å C18T, 55g $245 EAPP-30C18T/120G PuriFlash Column, 30µ PP 300Å C18T, 120g $490 EAPP-30C18T/175G PuriFlash Column, 30µ PP 300Å C18T, 175g $598 EAPP-30C18T/300G PuriFlash Column, 30µ PP 300Å C18T, 300g $1,040 EAPP-30C18T/450G PuriFlash Column, 30µ PP 300Å C18T, 450g $1,602 EAPP-30C18T/1250G PuriFlash Column, 30µ PP 300Å C18T, 1250g $4,191 EAPP-30C18T/2500G PuriFlash Column, 30µ PP 300Å C18T, 2500g $8,290 EA

PP-15C8/6G PuriFlash Column, 15µ PP 300Å C8, 6g $210 2/PKPP-15C8/20G PuriFlash Column, 15µ PP 300Å C8, 20g $210 EAPP-15C8/35G PuriFlash Column, 15µ PP 300Å C8, 35g $330 EAPP-15C8/55G PuriFlash Column, 15µ PP 300Å C8, 55g $521 EAPP-15C8/120G PuriFlash Column, 15µ PP 300Å C8, 120g $1,119 EAPP-15C8/175G PuriFlash Column, 15µ PP 300Å C8, 175g $1,640 EAPP-15C8/300G PuriFlash Column, 15µ PP 300Å C8, 300g $2,868 EAPP-15C8/450G PuriFlash Column, 15µ PP 300Å C8, 450g $4,429 EAPP-15C8/1250G PuriFlash Column, 15µ PP 300Å C8, 1250g $11,621 EAPP-15C8/2500G PuriFlash Column, 15µ PP 300Å C8, 2500g $23,198 EA

PP-30C8/6G PuriFlash Column, 30µ PP 300Å C8, 6g $170 2/PKPP-30C8/20G PuriFlash Column, 30µ PP 300Å C8, 20g $170 EAPP-30C8/35G PuriFlash Column, 30µ PP 300Å C8, 35g $205 EAPP-30C8/55G PuriFlash Column, 30µ PP 300Å C8, 55g $245 EAPP-30C8/120G PuriFlash Column, 30µ PP 300Å C8, 120g $490 EAPP-30C8/175G PuriFlash Column, 30µ PP 300Å C8, 175g $545 EAPP-30C8/300G PuriFlash Column, 30µ PP 300Å C8, 300g $1,005 EAPP-30C8/450G PuriFlash Column, 30µ PP 300Å C8, 450g $1,515 EAPP-30C8/1250G PuriFlash Column, 30µ PP 300Å C8, 1250g $3,687 EAPP-30C8/2500G PuriFlash Column, 30µ PP 300Å C8, 2500g $7,283 EA

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PuriFlash Reverse Phase Biopurification300Å C18 and C8 Bonded Silica

23PuriFlash Reverse Phase Biopurification300Å C4 and 200Å C18 Bonded Silica

Part number Description Price UnitPP-15C4/6G PuriFlash Column, 15µ PP 300Å C4, 6g $210 2/PKPP-15C4/20G PuriFlash Column, 15µ PP 300Å C4, 20g $215 EAPP-15C4/35G PuriFlash Column, 15µ PP 300Å C4, 35g $345 EAPP-15C4/55G PuriFlash Column, 15µ PP 300Å C4, 55g $545 EAPP-15C4/120G PuriFlash Column, 15µ PP 300Å C4, 120g $1,166 EAPP-15C4/175G PuriFlash Column, 15µ PP 300Å C4, 175g $1,708 EAPP-15C4/300G PuriFlash Column, 15µ PP 300Å C4, 300g $2,989 EAPP-15C4/450G PuriFlash Column, 15µ PP 300Å C4, 450g $4,618 EAPP-15C4/1250G PuriFlash Column, 15µ PP 300Å C4, 1250g $12,113 EAPP-15C4/2500G PuriFlash Column, 15µ PP 300Å C4, 2500g $24,178 EA

PP-30C4/6G PuriFlash Column, 30µ PP 300Å C4, 6g $170 2/PKPP-30C4/20G PuriFlash Column, 30µ PP 300Å C4, 20g $170 EAPP-30C4/35G PuriFlash Column, 30µ PP 300Å C4, 35g $205 EAPP-30C4/55G PuriFlash Column, 30µ PP 300Å C4, 55g $245 EAPP-30C4/120G PuriFlash Column, 30µ PP 300Å C4, 120g $490 EAPP-30C4/175G PuriFlash Column, 30µ PP 300Å C4, 175g $598 EAPP-30C4/300G PuriFlash Column, 30µ PP 300Å C4, 300g $1,040 EAPP-30C4/450G PuriFlash Column, 30µ PP 300Å C4, 450g $1,602 EAPP-30C4/1250G PuriFlash Column, 30µ PP 300Å C4, 1250g $4,191 EAPP-30C4/2500G PuriFlash Column, 30µ PP 300Å C4, 2500g $8,290 EA

PT-15C18T/6G PuriFlash Column, 15µ PT 200Å C18T, 6g $210 2/PKPT-15C18T/20G PuriFlash Column, 15µ PT 200Å C18T, 20g $224 EAPT-15C18T/35G PuriFlash Column, 15µ PT 200Å C18T, 35g $359 EAPT-15C18T/55G PuriFlash Column, 15µ PT 200Å C18T, 55g $565 EAPT-15C18T/120G PuriFlash Column, 15µ PT 200Å C18T, 120g $1,213 EAPT-15C18T/175G PuriFlash Column, 15µ PT 200Å C18T, 175g $1,776 EAPT-15C18T/300G PuriFlash Column, 15µ PT 200Å C18T, 300g $3,110 EAPT-15C18T/450G PuriFlash Column, 15µ PT 200Å C18T, 450g $4,800 EAPT-15C18T/1250G PuriFlash Column, 15µ PT 200Å C18T, 1250g $12,596 EAPT-15C18T/2500G PuriFlash Column, 15µ PT 200Å C18T, 2500g $25,150 EA

PT-30C18T/6G PuriFlash Column, 30µ PT 200Å C18T, 6g $170 2/PKPT-30C18T/20G PuriFlash Column, 30µ PT 200Å C18T, 20g $170 EAPT-30C18T/35G PuriFlash Column, 30µ PT 200Å C18T, 35g $205 EAPT-30C18T/55G PuriFlash Column, 30µ PT 200Å C18T, 55g $245 EAPT-30C18T/120G PuriFlash Column, 30µ PT 200Å C18T, 120g $490 EAPT-30C18T/175G PuriFlash Column, 30µ PT 200Å C18T, 175g $598 EAPT-30C18T/300G PuriFlash Column, 30µ PT 200Å C18T, 300g $1,040 EAPT-30C18T/450G PuriFlash Column, 30µ PT 200Å C18T, 450g $1,602 EAPT-30C18T/1250G PuriFlash Column, 30µ PT 200Å C18T, 1250g $4,191 EAPT-30C18T/2500G PuriFlash Column, 30µ PT 200Å C18T, 2500g $8,290 EA

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24

Part number Description Price UnitPT-15C8/6G PuriFlash Column, 15µ PT 200Å C8, 6g $210 2/PKPT-15C8/20G PuriFlash Column, 15µ PT 200Å C8, 20g $210 EAPT-15C8/35G PuriFlash Column, 15µ PT 200Å C8, 35g $330 EAPT-15C8/55G PuriFlash Column, 15µ PT 200Å C8, 55g $521 EAPT-15C8/120G PuriFlash Column, 15µ PT 200Å C8, 120g $1,119 EAPT-15C8/175G PuriFlash Column, 15µ PT 200Å C8, 175g $1,640 EAPT-15C8/300G PuriFlash Column, 15µ PT 200Å C8, 300g $2,868 EAPT-15C8/450G PuriFlash Column, 15µ PT 200Å C8, 450g $4,429 EAPT-15C8/1250G PuriFlash Column, 15µ PT 200Å C8, 1250g $11,621 EAPT-15C8/2500G PuriFlash Column, 15µ PT 200Å C8, 2500g $23,198 EA

PT-30C8/6G PuriFlash Column, 30µ PT 200Å C8, 6g $170 2/PKPT-30C8/20G PuriFlash Column, 30µ PT 200Å C8, 20g $170 EAPT-30C8/35G PuriFlash Column, 30µ PT 200Å C8, 35g $205 EAPT-30C8/55G PuriFlash Column, 30µ PT 200Å C8, 55g $245 EAPT-30C8/120G PuriFlash Column, 30µ PT 200Å C8, 120g $490 EAPT-30C8/175G PuriFlash Column, 30µ PT 200Å C8, 175g $592 EAPT-30C8/300G PuriFlash Column, 30µ PT 200Å C8, 300g $1,028 EAPT-30C8/450G PuriFlash Column, 30µ PT 200Å C8, 450g $1,587 EAPT-30C8/1250G PuriFlash Column, 30µ PT 200Å C8, 1250g $4,144 EAPT-30C8/2500G PuriFlash Column, 30µ PT 200Å C8, 2500g $8,199 EA

PT-15C4/6G PuriFlash Column, 15µ PT 200Å C4, 6g $210 2/PKPT-15C4/20G PuriFlash Column, 15µ PT 200Å C4, 20g $212 EAPT-15C4/35G PuriFlash Column, 15µ PT 200Å C4, 35g $342 EAPT-15C4/55G PuriFlash Column, 15µ PT 200Å C4, 55g $539 EAPT-15C4/120G PuriFlash Column, 15µ PT 200Å C4, 120g $1,154 EAPT-15C4/175G PuriFlash Column, 15µ PT 200Å C4, 175g $1,690 EAPT-15C4/300G PuriFlash Column, 15µ PT 200Å C4, 300g $2,954 EAPT-15C4/450G PuriFlash Column, 15µ PT 200Å C4, 450g $4,562 EAPT-15C4/1250G PuriFlash Column, 15µ PT 200Å C4, 1250g $11,971 EAPT-15C4/2500G PuriFlash Column, 15µ PT 200Å C4, 2500g $23,896 EA

PT-30C4/6G PuriFlash Column, 30µ PT 200Å C4, 6g $170 2/PKPT-30C4/20G PuriFlash Column, 30µ PT 200Å C4, 20g $170 EAPT-30C4/35G PuriFlash Column, 30µ PT 200Å C4, 35g $205 EAPT-30C4/55G PuriFlash Column, 30µ PT 200Å C4, 55g $245 EAPT-30C4/120G PuriFlash Column, 30µ PT 200Å C4, 120g $490 EAPT-30C4/175G PuriFlash Column, 30µ PT 200Å C4, 175g $598 EAPT-30C4/300G PuriFlash Column, 30µ PT 200Å C4, 300g $1,040 EAPT-30C4/450G PuriFlash Column, 30µ PT 200Å C4, 450g $1,602 EAPT-30C4/1250G PuriFlash Column, 30µ PT 200Å C4, 1250g $4,191 EAPT-30C4/2500G PuriFlash Column, 30µ PT 200Å C4, 2500g $8,290 EA

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PuriFlash Reverse Phase Biopurification200Å C8 & C4 Bonded Silica

25

Part number Description Price UnitCF-90A500/7ML PuriFlash Column, 90µ Cellufine DEAE, 7ml $47 2/PKCF-90A500/27ML PuriFlash Column, 90µ Cellufine DEAE, 27ml $71 EACF-90A500/45ML PuriFlash Column, 90µ Cellufine DEAE, 45ml $109 EACF-90A500/68ML PuriFlash Column, 90µ Cellufine DEAE, 68ml $168 EACF-90A500/145ML PuriFlash Column, 90µ Cellufine DEAE, 145ml $362 EACF-90A500/215ML PuriFlash Column, 90µ Cellufine DEAE, 215ml $527 EACF-90A500/375ML PuriFlash Column, 90µ Cellufine DEAE, 375ml $916 EACF-90A500/575ML PuriFlash Column, 90µ Cellufine DEAE, 575ml $1,414 EACF-90A500/1540ML PuriFlash Column, 90µ Cellufine DEAE, 1540ml $3,693 EACF-90A500/3100ML PuriFlash Column, 90µ Cellufine DEAE, 3100ml $7,295 EA

CF-90Q500/7ML PuriFlash Column, 90µ Cellufine QA, 7ml $53 2/PKCF-90Q500/27ML PuriFlash Column, 90µ Cellufine QA, 27ml $82 EACF-90Q500/45ML PuriFlash Column, 90µ Cellufine QA, 45ml $127 EACF-90Q500/68ML PuriFlash Column, 90µ Cellufine QA, 68ml $200 EACF-90Q500/145ML PuriFlash Column, 90µ Cellufine QA, 145ml $427 EACF-90Q500/215ML PuriFlash Column, 90µ Cellufine QA, 215ml $624 EACF-90Q500/375ML PuriFlash Column, 90µ Cellufine QA, 375ml $1,084 EACF-90Q500/575ML PuriFlash Column, 90µ Cellufine QA, 575ml $1,673 EACF-90Q500/1540ML PuriFlash Column, 90µ Cellufine QA, 1540ml $4,373 EACF-90Q500/3100ML PuriFlash Column, 90µ Cellufine QA, 3100ml $8,658 EA

CF-90C500/7ML PuriFlash Column, 90µ Cellufine CM, 7ml $47 2/PKCF-90C500/27ML PuriFlash Column, 90µ Cellufine CM, 27ml $71 EACF-90C500/45ML PuriFlash Column, 90µ Cellufine CM, 45ml $112 EACF-90C500/68ML PuriFlash Column, 90µ Cellufine CM, 68ml $171 EACF-90C500/145ML PuriFlash Column, 90µ Cellufine CM, 145ml $368 EACF-90C500/215ML PuriFlash Column, 90µ Cellufine CM, 215ml $536 EACF-90C500/375ML PuriFlash Column, 90µ Cellufine CM, 375ml $931 EACF-90C500/575ML PuriFlash Column, 90µ Cellufine CM, 575ml $1,437 EACF-90C500/1540ML PuriFlash Column, 90µ Cellufine CM, 1540ml $3,752 EACF-90C500/3100ML PuriFlash Column, 90µ Cellufine CM, 3100ml $7,410 EA

CF-75SO4/7ML PuriFlash Column, 75µ Cellufine SO4, 7ml $124 EACF-75SO4/27ML PuriFlash Column, 75µ Cellufine SO4, 27ml $430 EACF-75SO4/45ML PuriFlash Column, 75µ Cellufine SO4, 45ml $692 EACF-75SO4/68ML PuriFlash Column, 75µ Cellufine SO4, 68ml $1,096 EACF-75SO4/145ML PuriFlash Column, 75µ Cellufine SO4, 145ml $2,350 EACF-75SO4/215ML PuriFlash Column, 75µ Cellufine SO4, 215ml $3,443 EACF-75SO4/375ML PuriFlash Column, 75µ Cellufine SO4, 375ml $6,034 EACF-75SO4/575ML PuriFlash Column, 75µ Cellufine SO4, 575ml $9,318 EACF-75SO4/1540ML PuriFlash Column, 75µ Cellufine SO4, 1540ml ###### EACF-75SO4/3100ML PuriFlash Column, 75µ Cellufine SO4, 3100ml ###### EA

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PuriFlash Cellufine BiopurificationIon Exchange and Affinity

QuoteQuote

26 PuriFlash Cellufine BiopurificationEndotoxin Removal and Hydrophobic Interaction

Part number Description Price UnitCF-90EL/7ML PuriFlash Column, 75µ Cellufine Etclean L, 7ml $153 EACF-90EL/27ML PuriFlash Column, 75µ Cellufine Etclean L, 27ml $527 EACF-90EL/45ML PuriFlash Column, 75µ Cellufine Etclean L, 45ml $851 EACF-90EL/68ML PuriFlash Column, 75µ Cellufine Etclean L, 68ml $1,346 EACF-90EL/145ML PuriFlash Column, 75µ Cellufine Etclean L, 145ml $2,892 EACF-90EL/215ML PuriFlash Column, 75µ Cellufine Etclean L, 215ml $4,235 EACF-90EL/375ML PuriFlash Column, 75µ Cellufine Etclean L, 375ml $7,427 EACF-90EL/575ML PuriFlash Column, 75µ Cellufine Etclean L, 575ml ###### EACF-90EL/1540ML PuriFlash Column, 75µ Cellufine Etclean L, 1540ml ###### EACF-90EL/3100ML PuriFlash Column, 75µ Cellufine Etclean L, 3100ml ###### EA

CF-75ES/7ML PuriFlash Column, 75µ Cellufine Etclean S, 7ml $153 EACF-75ES/27ML PuriFlash Column, 75µ Cellufine Etclean S, 27ml $527 EACF-75ES/45ML PuriFlash Column, 75µ Cellufine Etclean S, 45ml $851 EACF-75ES/68ML PuriFlash Column, 75µ Cellufine Etclean S, 68ml $1,346 EACF-75ES/145ML PuriFlash Column, 75µ Cellufine Etclean S, 145ml $2,892 EACF-75ES/215ML PuriFlash Column, 75µ Cellufine Etclean S, 215ml $4,235 EACF-75ES/375ML PuriFlash Column, 75µ Cellufine Etclean S, 375ml $7,427 EACF-75ES/575ML PuriFlash Column, 75µ Cellufine Etclean S, 575ml ###### EACF-75ES/1540ML PuriFlash Column, 75µ Cellufine Etclean S, 1540ml ###### EACF-75ES/3100ML PuriFlash Column, 75µ Cellufine Etclean S, 3100ml ###### EA

CF-90C4/7ML PuriFlash Column, 90µ Cellufine C4, 7ml $65 EACF-90C4/27ML PuriFlash Column, 90µ Cellufine C4, 27ml $215 EACF-90C4/45ML PuriFlash Column, 90µ Cellufine C4, 45ml $342 EACF-90C4/68ML PuriFlash Column, 90µ Cellufine C4, 68ml $539 EACF-90C4/145ML PuriFlash Column, 90µ Cellufine C4, 145ml $1,157 EACF-90C4/215ML PuriFlash Column, 90µ Cellufine C4, 215ml $1,696 EACF-90C4/375ML PuriFlash Column, 90µ Cellufine C4, 375ml $2,966 EACF-90C4/575ML PuriFlash Column, 90µ Cellufine C4, 575ml $4,579 EACF-90C4/1540ML PuriFlash Column, 90µ Cellufine C4, 1540ml ###### EACF-90C4/3100ML PuriFlash Column, 90µ Cellufine C4, 3100ml ###### EA

CF-90PH/7ML PuriFlash Column, 90µ Cellufine Phenyl, 7ml $65 EACF-90PH/27ML PuriFlash Column, 90µ Cellufine Phenyl, 27ml $215 EACF-90PH/45ML PuriFlash Column, 90µ Cellufine Phenyl, 45ml $342 EACF-90PH/68ML PuriFlash Column, 90µ Cellufine Phenyl, 68ml $539 EACF-90PH/145ML PuriFlash Column, 90µ Cellufine Phenyl, 145ml $1,157 EACF-90PH/215ML PuriFlash Column, 90µ Cellufine Phenyl, 215ml $1,696 EACF-90PH/375ML PuriFlash Column, 90µ Cellufine Phenyl, 375ml $2,966 EACF-90PH/575ML PuriFlash Column, 90µ Cellufine Phenyl, 575ml $4,579 EACF-90PH/1540ML PuriFlash Column, 90µ Cellufine Phenyl, 1540ml ###### EACF-90PH/3100ML PuriFlash Column, 90µ Cellufine Phenyl, 3100ml ###### EA

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27PuriFlash Normal Phase Jumbo Packs HP: High Performance Silica

Part number Description Price UnitPF-15SIHP-JP/4G PuriFlash Column, 15µ Si HP, 4g $836 80/PKPF-15SIHP-JP/12G PuriFlash Column, 15µ Si HP, 12g $2,014 80/PKPF-15SIHP-JP/25G PuriFlash Column, 15µ Si HP, 25g $1,843 48/PKPF-15SIHP-JP/40G PuriFlash Column, 15µ Si HP, 40g $2,717 48/PKPF-15SIHP-JP/80G PuriFlash Column, 15µ Si HP, 80g $1,919 16/PKPF-15SIHP-JP/120G PuriFlash Column, 15µ Si HP, 120g $2,755 16/PKPF-15SIHP-JP/200G PuriFlash Column, 15µ Si HP, 200g $2,356 8/PKPF-15SIHP-JP/300G PuriFlash Column, 15µ Si HP, 300g $2,945 8/PKPF-15SIHP-JP/800G PuriFlash Column, 15µ Si HP, 800g $4,655 4/PKPF-15SIHP-JP/1600G PuriFlash Column, 15µ Si HP, 1600g $8,930 4/PK

PF-30SIHP-JP/4G PuriFlash Column, 30µ Si HP, 4g $1,406 160/PKPF-30SIHP-JP/12G PuriFlash Column, 30µ Si HP, 12g $1,463 120/PKPF-30SIHP-JP/25G PuriFlash Column, 30µ Si HP, 25g $1,881 100/PKPF-30SIHP-JP/40G PuriFlash Column, 30µ Si HP, 40g $2,014 80/PKPF-30SIHP-JP/80G PuriFlash Column, 30µ Si HP, 80g $1,577 40/PKPF-30SIHP-JP/120G PuriFlash Column, 30µ Si HP, 120g $1,710 32/PKPF-30SIHP-JP/200G PuriFlash Column, 30µ Si HP, 200g $1,520 16/PKPF-30SIHP-JP/300G PuriFlash Column, 30µ Si HP, 300g $2,413 16/PKPF-30SIHP-JP/800G PuriFlash Column, 30µ Si HP, 800g $1,691 4/PKPF-30SIHP-JP/1600G PuriFlash Column, 30µ Si HP, 1600g $2,983 4/PK

PF-50SIHP-JP/4G PuriFlash Column, 50µ Si HP, 4g $1,007 160/PKPF-50SIHP-JP/12G PuriFlash Column, 50µ Si HP, 12g $1,121 120/PKPF-50SIHP-JP/25G PuriFlash Column, 50µ Si HP, 25g $1,178 100/PKPF-50SIHP-JP/40G PuriFlash Column, 50µ Si HP, 40g $1,178 80/PKPF-50SIHP-JP/80G PuriFlash Column, 50µ Si HP, 80g $851 40/PKPF-50SIHP-JP/120G PuriFlash Column, 50µ Si HP, 120g $1,368 32/PKPF-50SIHP-JP/200G PuriFlash Column, 50µ Si HP, 200g $1,045 16/PKPF-50SIHP-JP/300G PuriFlash Column, 50µ Si HP, 300g $1,577 16/PKPF-50SIHP-JP/800G PuriFlash Column, 50µ Si HP, 800g $950 4/PKPF-50SIHP-JP/1600G PuriFlash Column, 50µ Si HP, 1600g $1,482 4/PK

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HC: High Capacity SilicaPart number Description Price UnitPF-15SIHC-JP/4G PuriFlash Column, 15µ Si HC, 4G $836 80/PKPF-15SIHC-JP/12G PuriFlash Column, 15µ Si HC, 12G $2,014 80/PKPF-15SIHC-JP/25G PuriFlash Column, 15µ Si HC, 25G $1,843 48/PKPF-15SIHC-JP/40G PuriFlash Column, 15µ Si HC, 40G $2,717 48/PKPF-15SIHC-JP/80G PuriFlash Column, 15µ Si HC, 80G $1,919 16/PKPF-15SIHC-JP/120G PuriFlash Column, 15µ Si HC, 120g $2,755 16/PKPF-15SIHC-JP/200G PuriFlash Column, 15µ Si HC, 200g $2,356 8/PKPF-15SIHC-JP/300G PuriFlash Column, 15µ Si HC, 300g $2,945 8/PKPF-15SIHC-JP/800G PuriFlash Column, 15µ Si HC, 800g $4,655 4/PKPF-15SIHC-JP/1600G PuriFlash Column, 15µ Si HC, 1600g $8,930 4/PK

PF-25SIHC-JP/4G PuriFlash Column, 25µ Si HC, 4g $1,406 160/PKPF-25SIHC-JP/12G PuriFlash Column, 25µ Si HC, 12g $1,463 120/PKPF-25SIHC-JP/25G PuriFlash Column, 25µ Si HC, 25g $1,881 100/PKPF-25SIHC-JP/40G PuriFlash Column, 25µ Si HC, 40g $2,014 80/PKPF-25SIHC-JP/80G PuriFlash Column, 25µ Si HC, 80g $1,577 40/PKPF-25SIHC-JP/120G PuriFlash Column, 25µ Si HC, 120g $1,710 32/PKPF-25SIHC-JP/200G PuriFlash Column, 25µ Si HC, 200g $1,520 16/PKPF-25SIHC-JP/300G PuriFlash Column, 25µ Si HC, 300g $2,413 16/PKPF-25SIHC-JP/800G PuriFlash Column, 25µ Si HC, 800g $1,691 4/PKPF-25SIHC-JP/1600G PuriFlash Column, 25µ Si HC, 1600g $2,983 4/PK

PF-50SIHC-JP/4G PuriFlash Column, 50µ Si HC, 4g $1,045 160/PKPF-50SIHC-JP/12G PuriFlash Column, 50µ Si HC, 12g $1,159 120/PKPF-50SIHC-JP/25G PuriFlash Column, 50µ Si HC, 25g $1,216 100/PKPF-50SIHC-JP/40G PuriFlash Column, 50µ Si HC, 40g $1,216 80/PKPF-50SIHC-JP/80G PuriFlash Column, 50µ Si HC, 80g $1,273 40/PKPF-50SIHC-JP/120G PuriFlash Column, 50µ Si HC, 120g $1,406 32/PKPF-50SIHC-JP/200G PuriFlash Column, 50µ Si HC, 200g $1,178 16/PKPF-50SIHC-JP/300G PuriFlash Column, 50µ Si HC, 300g $1,615 16/PKPF-50SIHC-JP/800G PuriFlash Column, 50µ Si HC, 800g $988 4/PKPF-50SIHC-JP/1600G PuriFlash Column, 50µ Si HC, 1600g $2,014 4/PK

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Part number Description Price UnitPF-15SIHC-JP/4G PuriFlash Column, 15µ Si HC, 4G $836 80/PKPF-15SIHC-JP/12G PuriFlash Column, 15µ Si HC, 12G $2,014 80/PKPF-15SIHC-JP/25G PuriFlash Column, 15µ Si HC, 25G $1,843 48/PKPF-15SIHC-JP/40G PuriFlash Column, 15µ Si HC, 40G $2,717 48/PKPF-15SIHC-JP/80G PuriFlash Column, 15µ Si HC, 80G $1,919 16/PKPF-15SIHC-JP/120G PuriFlash Column, 15µ Si HC, 120g $2,755 16/PKPF-15SIHC-JP/200G PuriFlash Column, 15µ Si HC, 200g $2,356 8/PKPF-15SIHC-JP/300G PuriFlash Column, 15µ Si HC, 300g $2,945 8/PKPF-15SIHC-JP/800G PuriFlash Column, 15µ Si HC, 800g $4,655 4/PKPF-15SIHC-JP/1600G PuriFlash Column, 15µ Si HC, 1600g $8,930 4/PK



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Part number Description Price UnitPF-15SIHC-JP/4G PuriFlash Column, 15µ Si HC, 4G $836 80/PKPF-15SIHC-JP/12G PuriFlash Column, 15µ Si HC, 12G $2,014 80/PKPF-15SIHC-JP/25G PuriFlash Column, 15µ Si HC, 25G $1,843 48/PKPF-15SIHC-JP/40G PuriFlash Column, 15µ Si HC, 40G $2,717 48/PKPF-15SIHC-JP/80G PuriFlash Column, 15µ Si HC, 80G $1,919 16/PKPF-15SIHC-JP/120G PuriFlash Column, 15µ Si HC, 120g $2,755 16/PKPF-15SIHC-JP/200G PuriFlash Column, 15µ Si HC, 200g $2,356 8/PKPF-15SIHC-JP/300G PuriFlash Column, 15µ Si HC, 300g $2,945 8/PKPF-15SIHC-JP/800G PuriFlash Column, 15µ Si HC, 800g $4,655 4/PKPF-15SIHC-JP/1600G PuriFlash Column, 15µ Si HC, 1600g $8,930 4/PK



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PuriFlash Column Specifications

[email protected] • 800.560.8262www.interchiminc.com

©2012 Interchim Inc. All prices subject to change. 121031

Column TypeSorbent Weight

ID (mm)

Length (mm) Volume

Max Pressure

Typical Flow Rate

Flow Range

4g Si, 6g Bonded 12 62 7 ml

22 bar / 320 PSI 7ml/min

5-20 ml/min

12g Si, 20g

Bonded 21 78 27 ml22 bar / 320 PSI

20 ml/min

15-50 ml/min

25g Si, 35g


20 ml/min

15-50 ml/min

40g Si, 55g


30 ml/min

20-70 ml/min

80g Si, 120g

Bonded 31 193 145 ml20 bar / 290 PSI

40 ml/min

30-100 ml/min

120g Si, 175g

Bonded 36 212 215 ml20 bar / 290 PSI

60 ml/min

40-150 ml/min

200g Si, 300g

Bonded 60 133 375 ml20 bar / 290 PSI

160 ml/min

80-300 ml/min

300g Si, 450g

Bonded 60 205 575 ml20 bar / 290 PSI

160 ml/min

80-300 ml/min

800g Si, 1250g

Bonded 78 323 1540 ml7 bar /

100 PSI200

ml/min150-300 ml/min

1600g Si, 2500g

Bonded 104 365 3100 ml7 bar /

100 PSI400

ml/min200-500 ml/min

0800

0300

0200

0120

0080

0040

0025

0012

0004

1600

For as low as $25k, the puriFlash 215 is a fully automated purification instrument with UV triggered collection. Whether you are looking for Flash or Prep HPLC, Interchim has an affordable instru-ment to suit your needs.

HPLC pump technology is used on all puriFlash instruments to provide years of reliability with precise gradient control. Multiple detection options include UV, RI and ELSD (for non-UV adsorb-ing samples). Mass directed purification is now available for Flash and Prep HPLC.

Flash & Preparative HPLC on a single instrument

Lab Scale Flash Lab Scale Flash & Prep HPLC Large Scale FlashPuriFlash PF215 & PF430 PuriFlash PF800PuriFlash PF450, PF4100 & PF4250

Flash sample loading from 10mg to 160g

Flash Columns up to 800 g

PF215: 200ml/min, 215 PSI PF430: 200ml/min, 435 PSI

Flash sample loading from 10mg to 600g

Flash Columns up to 3.0kg

PF800: 800ml/min, 145 PSI

Flash sample loading:10mg to 160g Reverse Phase Loading: 10mg to 5g

Flash Columns up to 800 g HPLC Columns up to 75mm ID

PF450: 250ml/min, 725 PSI PF4100: 250ml/min, 1450 PSI PF4250: 40 or 250 ml/min, 3625 PSI

Purification based software for Flash & Prep HPLC

HPLC pump with four solvents for a true

quaternary gradient

UV DAD Multi-Wavelength

-500 to 50,000mAU

Automated Sample

Injection

Flash Columns 4g to 2.5kg

HPLC Columns 10 to 75mm ID

High Capacity Fraction Collector Save hood space with the fume enclosure option

The modular design of the PuriFlash product line allows for upgrad-ing to a different pump, detector or collector at any time in the future

Contact Interchim and book a demo today: 800-560-8262

29

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4

12

25

40

80

120

200

300

800

1600

10 100

Sample Size (mg)

1000 10,000 100,000 1,000,000

TLC is used to determine optimum solvent compositions for flash chromatography. Since flow cannot be controlled, retention is measured in distance rather than time or column volume. The Retention Factor, Rf, is defined as the distance traveled and ∆Rf is the distance between the compounds. A suitable solvent system is one that moves the compound to an Rf of 0.1 - 0.3 and will separate the compound from others by a ∆Rf of at least 0.1. Unlike TLC, flash chromatography is governed by column volumes (CV). CV is the number of column volumes required to elute the compound from the column. ∆CV is the number of column volumes between the compounds. A suitable flash solvent system is one which elutes the com-pound in 3-6 CV and will separate compounds by a ∆CV greater than 1. The loading chart below provides guidelines based on sample load and ∆CV to determine the appropri-ate sized column. The chart is based on using 50µ HC silica columns under normal phase conditions. Expect 2-3 times higher loading with 25µ and 15µ silica.

TLC to Flash Optimization

Interchim provides a wide variety of high performance TLC plates to match the column offering. To trans-pose a TLC run to a Flash run, the same type of silica/sorbent should be used for both techniques.

∆Rf = Rf B - Rf A CV = 1/Rf ∆CV = 1/Rf A - 1/Rf BRf = Distance travelled by the compoundDistance travelled by the solvent front

∆CV∆CV = (1 to 1.5) - Difficult

∆CV = (1.5 to 4) - Standard

∆CV = (4 to 10) - EasyOrigin

Solvent 60mm

A=10mm

B=18mm

Rf A = 0.16

Rf B = 0.3

CVA = 6.25 CVB = 3.33∆CV = 2.9

∆Rf = .14

Puriflash Columns Part No. TLC Plate Unit Price SIHP, SIHC PFT-1000 Silica Gel, 2.5 x 7.5cm 100PK $185 C18HP, C18HC, C18HQ PFT-1001 C18, 2.5 x 7.5cm 50PK $775 RPAQ, PP-C18T, PT-C18TPP-C8, PT-C8 PFT-15684-1 C8, acid stable, 5 x 10cm 25PK $435 ALN PFT-1002 Alumina, 2.5 x 7.5cm 100PK $295 CN PFT-1003 CN (Cyano), 2.5 x 7.5cm 50PK $775 Diol PFT-1004 Diol, 2.5 x 7.5cm 50PK $775 NH2, NH2HC PFT-1005 NH2 (Amino), 2.5 x 7.5cm 50PK $775 PFT-15683-3 C18, acid stable, 5 x 20cm 50PK $616 PFT-GC-05 Spotting Capillary, 0.5ul, 32mm 100PK $75Part Number TLC Plates Particle Size Layer Thickness Surface AreaPFT-1000 Silica Gel, 2.5 x 7.5cm 5-17µ 250µ 500 m2/gPFT-1001 C18, 2.5 x 7.5cm 5-17µ 250µ 500 m2/gPFT-15684-1 C8, acid stable, 5 x 10cm 10-12µ 230µ 520 m2/gPFT-1002 Alumina, 2.5 x 7.5cm 250µ 200 m2/gPFT-1003 CN (Cyano), 2.5 x 7.5cm 2-10µ 200µ 500 m2/gPFT-1004 Diol, 2.5 x 7.5cm 2-10µ 200µ 500 m2/gPFT-1005 NH2 (Amino), 2.5 x 7.5cm 2-10µ 200µ 500 m2/gPFT-15683-3 C18, acid stable, 5 x 20cm 10-12µ 230µ 520 m2/g

1 10 100 1,000 10,000 100,000 1,000,000

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