qin et al., sup fig. 1 a fsc-h ssc-w fsc-a ssc-a 92.7% 23 ......qin et al., sup fig. 3 medium...
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-
FSC-A
SSC
-A
Live/Dead
SSC
-A
FSC-A
FSC
-H
SSC-A
SSC
-W
92.7%
CD11c
SSC
-A
CD11b
Gr1
52.7%
23.2%
4.57%
CD11c
MH
IIC
4.47%
CD3
NK
1.1
2.72%
5.29%
CD19
CD
34.71%
6.52%
P2X7
SSC
-A
Neu/mono
23.1%
P2X7
SSC
-A
DC
3.26%
P2X7
SSC
-A
NK
3.41%
P2X7
SSC
-A
T cells
CD45SS
C-A
3.85%
P2X7
SSC
-AB cells
Qin et al., Sup Fig. 1A
B
84.6%
F4/80
SSC
-A
83.2%
P2X7
SSC
-A
TAM
P2X7
+ CD
68+
cells
/fiel
d
P2X7CD68
DAPI MergeHuman
50μm
-
Qin et al., Sup Fig. 2A B
D
C
ID8 intraperitoneal injection
P2X7+/+
P2X7-/-
12.1% 6.15%
F4/80-APC
CD
206-
PE
P2X7+/+ P2X7-/-
Orthotopic
P2X7+/+
P2X7-/-
P2X7+/+
P2X7-/-
Ort
hoto
pic
CD206CD68DAPI Merge
Perc
ent s
urvi
val
Day
P2X7+/+P2X7-/-
P=0.0485
E
G
F
Mill
ilite
rs
Ascites volume
P2X7+/+ P2X7-/-
Perc
ent o
f M2
mac
roph
age
(%)
P2X7+/+ P2X7-/-
0
20
40
60
80
100 ***
P2X7+/+ P2X7-/-
CD
206+
/F4/
80+ (
%)
100μm
100μm
50μm
50μm
-
Qin et al., Sup Fig. 3
Medium LPS+IFNγ
F4/80
CD
16/3
2
16.7%
15.9%
44.7%
44.2%
P2X7+/+
P2X7-/-
B
D
CA
P2X7+/+P2X7-/-
Rel
ativ
e ex
pres
sion
of i
NO
S
Control LPS+IFNγ
BMDMP2X7+/+P2X7-/-
Rel
ativ
e ex
pres
sion
of T
NFα
Control LPS+IFNγ
BMDM
P2X7+/+P2X7-/-
0
20
40
60
80
100ns
Rel
ativ
e ex
pres
sion
of C
XCL1
0
Control LPS+IFNγ
BMDM
P2X7+/+P2X7-/-
Perc
ent o
f M1
mac
roph
ages
(%)
Control LPS+IFNγ
BMDM
-
Qin et al., Sup Fig. 4BA C
Rel
ativ
e ex
pres
sion
of F
izzl
ControlLLC-CMLLC-CM+ O-ATP
E
D
F G
K L M
H
Rel
ativ
e ex
pres
sion
of A
rg1
MH-S
Rel
ativ
e ex
pres
sion
of F
izzl
MH-S
MFI
Nor
mal
ized
to M
ode
CD206
MH-S
Control IL4 IL4+ O-ATPIL4+ A-43 IL4+ A-74
Rel
ativ
e ex
pres
sion
of A
rg1
ControlIL4IL4+ O-ATP
BMDM
BMDM
Rel
ativ
e ex
pres
sion
of C
D20
6 ControlLLC-CMLLC-CM+ O-ATP
BMDM
Rel
ativ
e ex
pres
sion
of A
rg1
ControlLLC-CMLLC-CM+ O-ATP
BMDM
Control
Rel
ativ
e ex
pres
sion
of F
izzl IL4
IL4+ O-ATP
BMDM
Rel
ativ
e ex
pres
sion
of C
D20
6 ControlIL4IL4+ O-ATP
BMDM
I J
Control IL4 IL4+ O-ATP IL4+ A-43 IL4+ A-74
Rel
ativ
e ex
pres
sion
of C
D20
6
MHS
0
50
100
150
200
250*** *
**
Rel
ativ
e ex
pres
sion
of A
rg1
0
1000
2000
3000
4000*** * ***
Rel
ativ
e ex
pres
sion
of F
izzl
Con
trol
IL4
IL4+
A-4
3
IL4+
A-7
4
F4/80
CD
206
4.35% 27.4%
15.7% 9.7%
BMDMControl IL4 IL4+ A-43 IL4+ A-74
BMDM
-
Qin et al., Sup Fig. 5A
B
0.913% 10% 8.77%8.47%
IFNγ
0.906% 7.48% 8.70%9.63%
TNFα
CD
4+T
cells
α-CD3/CD28Controlα-CD3/CD28
+WT Mϕα-CD3/CD28+P2X7-/- Mϕ
%IF
Nγ
+C
D4+
T c
ells
%TN
Fα+
CD
4+T
cells
Control α-CD3/CD28 α-CD3/CD28+WT Mϕα-CD3/CD28+P2X7-/- Mϕ
-
A B C
PBS
A-43
Cisplatin
A-43+Cis
A-74+Cis
A-74
Tum
or w
eigh
t (g)
Qin et al., Sup Fig. 6
Volu
me
of tu
mor
(mm
3 ) PBS
A-43Cisplatin
A-43+ Cis
Day
A-74+ CisA-74
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