quality control records - nshnsh.org/sites/default/files/qualtiy_control_records.pdf · quality...

QUALITY CONTROL RECORDS

National Society for Histotechnology 4201 Northview Drive, Suite 502

Bowie, Maryland 20716-2604 Phone: 301-262-6221 Fax: 301-262-9188

email: [email protected] website: www.nsh.org

mailto:[email protected]

http://www.nsh.org/

Quality Control Record sheet

Tissue Specimen

Date

Pathology #

Specimen

# Pieces/ Cassette

# H&E

# Special Stains

Pathologist/ Technician

8/18/86

S86-2001

Skin-Leg

1

1

3

DC/AF

“

S-86-2002

Liver-bx

1

1

5

“

“

S86-2003

T & A

1

1

0

“

“

S86-2004a

Uterus

1

1

0

“

“

S86-2004b

Tubes

2

1

0

“

“

S86-2004c

Ovary

1

1

0

“

1


Tissue Specimen

Date

Pathology #

Specimen

# Pieces/ Cassette

# H&E

# Special Stains

Pathologist/ Technician

2

Quality Control Sheet

Slide Evaluation Checklist

Slide ID# __________Kidney – 43 ____________ Preparer__________AF___________________ Date ___________9/5/86____________________ Evaluator _______DD2___________________

SLIDE APPEARANCE ACCEPTABLE NOTACCEPTABLE COMMENTS

Label position X

Label Information X

Coverslip position X

Mounting medium X-none

Slide (colored) X

Tissue section position X-none

Air bubbles X-none

PROCESSING ARTIFACTS Fixation X

Decalcification NA NA = Not Applicable

Fixative pigments X-none

Cell shrinkage X-none

Others

MICROTOMING

Thickness

Knife Lines X-none

Incomplete X

Floaters few

Compression/Folds minor

Others

STAINING Nuclear Stain X

Cytoplasmic stain X Collagen & smooth muscle not distinguishable

Special stain element

Counterstain

Staining artifacts

3

Quality Control Sheet Slide Evaluation Checklist

Slide ID # _________________________________ Preparer __________________________________

Date _____________________________________ Evaluator _________________________________

SLIDE APPEARANCE ACCEPTABLE NOT ACCEPTABLE COMMENTS

Label Position

Label Information

Coverslip position

Mounting medium

Slide (colored)

Tissue section Position

Air bubbles

PROCESSING ARTIFACTS

Fixation

Decalcification

Fixative pigments

Cell shrinkage

Others

MICROTOMING

Thickness

Knife Lines

Incomplete

Floaters

Compression/Folds

Others

STAINING

Nuclear Stain

Cytoplasmic stain

Special stain element

Counterstain

Staining artifacts

4

Quality Control Record sheet: Automatic Tissue Processor

Type of Processor: Closed Week of: August 17, 1986

Station # Day) Solution Date) Runs/Day)

8/18 M

__1__

8/19 Tu

__1__

8/20 W

__1__

8/21 Th

__2__

8/22 F

__1__

8/23 Sa

__0__

_____ Su

_____ 1 Rotate/Discard NFB Qc Check

New Ph-7

Discard Discard

2 Rotate/Discard NFB QC Check

New Ph-7

Rotate- New

Rotate- New

3 Rotate/Discard 80% alc QC Check

New 80%

Discard- New

Rotate- New


New 95%

Discard Discard


New 95%

Rotate- New

Rotate- New


New 100%

Discard Discard

7 Rotate/Discard 100% alc Qc Check

New 100%

Rotate Rotate


New 100%

Rotate New

Rotate New

9 Rotate/Discard xyl QC Check

New Discard Discard

10 Rotate/Discard xyl QC Check

New Rotate- New

Rotate- New

11 Rotate/Discard Paraffin QC Check

New

12 Rotate/Discard Paraffin QC Check

New

13 Rotate/Discard ______ QC Check

Purge 1 Rotate/Discard xyl

Used 2X

Used 2X

Used 2X

Used 4X Discard

New Used 2X

Purge 2 Rotate/Discard 100% alc

Used 2X

Used 2X

Used 2X

Used 4X Discard

New Used 2X

Initials

AF

AF

AF

AF

DD

5

Quality Control Record sheet: Automatic Tissue Processor

Type of Processor _____________________________ Week of ____________________________

Station # Day) Solution Date) Runs/Day)

M

_________

T

_________

W

__________

TH

__________

F

__________

Sa

__________

Su

_________

1 Rotate/Discard ________ QC Check








9 Rotate Discard ________ QC Check





Purge 1 Rotate/Discard ________

Purge 2 Rotate/Discard ________

Initials

6

Quality Control Record sheet: Decals

Decal Solution: 5% Formic Acid

Date Pathology #

Fixative Decal Started

Decal Ended

Comments/Initials

6/24/86 A86-102 NBF 6/25/86 6/27/86 Decal charged 6/26/86 Cut OK AF

7/01/86 S86-212 NBF 7/01/86 7/02/86 Tissue gritty while microtoming AF

7

Quality Control Record sheet: Decals Decal Solution: _________________________________

Date Pathology #

Fixative Decal Started

Decal Ended

Comments/Initials

8

Quality Control Record sheet: Routine Stain Solutions

Week of: August 17, 1986 D – Discard F – Filtered N – New R – Rotated

Day) Solution Date) Slides/Day)

8/18 M

200

8/19 T

150

8/20 W

100

8/21 Th 30

8/22 F

100

8/23 Sa 0

Su

_____ Rotate/Discard xyl

N D D D D

Rotate/Discard xyl

N R R R R

Rotate/Discard xyl

N R/N R/N R/N R/N

Rotate/Discard 100%

N D D D D

Rotate Discard 100%

N R R R R

Rotate/Discard 100%

N R/N R/N R/N R/N

Rotate/Discard 95% QC Check

N - - - -

Hema- Rotate/Discard toxylin QC Check

N F Ph 2.8

F Ph 2.8

F Ph 2.8

F Ph 2.8

Rotate/Discard Acid alc QC Check

N N N

Rotate/Discard Blueing QC Check

N N N


N - -

Rotate/Discard Eosin QC Check

N F Ph 4.5

F Ph 4.5

F Ph 4.5

F Ph 4.5


N D D - D

Rotate/Discard 100%

N R R - R

Rotate/Discard 100%

N R/N R/N - R/N

Rotate/Discard xyl

N D D - D

Rotate/Discard xyl

N R R - R

Rotate/Discard xyl

N R/N R/N - R/N

Initials

AF

AF

AF

AF

AF

9

Quality Control Record sheet: Routine Stain Solutions Week of: _________________________________________ D – Discard, F – Filtered, N – New, R – Rotated

Day) Solution Date) Slides/Day)

M

______

T

______

W

______

Th

______

F

______

Sa

______

Su

______ Rotate/Discard __________

Rotate/Discard __________





Rotate/Discard __________ QC Check











Initials


Special Stain for

Control Block ID S86-32 Date 8/06/86 to

Date

Pathology #

Source

Fixative

Control

+ / -

Unknown

+ / -

Technician

8/06/86 S-86-786 Liver NBF + + AF

8/08/86 S-86-807 Bone Marrow Bouins + + AF

8/08/86 S-86-811 Colon (tumor) NBF + - AF

11


Special Stain for

Control Block ID Date to

Date

Pathology #

Source

Fixative

Control

+ / -

Unknown

+ / -

Tehnician

12

Quality Control Record sheet: Temperature Control

Month/Year July, 1986

Instrument: Acceptable Range:

DATE

Slide Dryer

58-62° C

Oven

35-39° C

Embedding Center

58-62° C1 60° 37° 60°

2 60° 37° 60°

3 60° 37° 60°

4 - 37° -

5 - - -

6 - - -

7 59° - 61°

8 60° 37° 61°

9 59° 37° 61°

10 59° 37° 61

11 37°

12

13

14

15 60° 37° 61°

16

17

18

19

20

21

22

23 60° 37° 60°

24

25

26

27

28

29

30 60° 37° 60°

31

13

Quality Control Record sheet: Temperature Control

Month/Year

Instrument: Acceptable Range:

DATE

Slide Dryer

_______________

Oven

_______________

_______________

_______________

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

14

Quality Control Record sheet Procedure Manual Changes

Effective

Date Problem/Procedure Addition/Correction Implemented Initials

6/23/86

Tissues too hard: crunchy

Change station 3 on tissue processor from 95 % alcohol to 80 % alcohol

AF

Results: Better. Fibrous tissues still difficult to cut

6/24/86

Fibrous tissue hard Change station 3 on tissue processor from 80 % alcohol to 70 % alcohol

AF

Results: Fibrous tissues - OK

7/11/86

Eosin too dark Rinse in 95 % alcohol for 1 minute, Post eosin

AF

Results: OK. Slides lighter in eosin

15

Quality Control Record sheet Procedure Manual Changes

Effective Date

Problem/Procedure Addition/Correction Implemented Initials

16

Reviewed 2001

quality control records - nshnsh.org/sites/default/files/qualtiy_control_records.pdf · quality...

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