rAAV2-Cont shRNA

rAAV2-mTOR shRNA

mTOR

P-mTOR(S2448)

P- p70S6K(T389)

GFP

actin

Cont mTOR Cont mTOR

2 days 4 days

1. rAAV2-Cont shRNA (Cont shRNA)2. rAAV2-mTOR shRNA (mTOR shRNA)

Bi-directional promoter

shRNA pH1 pCMV GFP pol A

a

c

b

d

2D 4D0.0

0.2

0.4

0.6

0.8

1.0

1.2

Cont shRNA mTOR shRNA

Re

lati

ve

am

ou

nt

of

mT

OR

R

NA

ex

pre

ss

ion

Supplemental Fig. 2

*

**

P-mTOR(S2481)

P- AKT(S473)