radiation-resistance? there's a gene for that
DESCRIPTION
Radiation-resistance? There's a Gene for That. New York City Wetware iGEM Team. Extraordinary Biology Numbers. Extremophile Numbers Extraordinary Synthetic Biology Numbers. 130 MPa. 130 Celsius. 1.08-mega-base pair. 4 Days. Deinococcus radiodurans (D. rad). 12,000 Gray!!!!!. - PowerPoint PPT PresentationTRANSCRIPT
Radiation-resistance?
There's a Gene for That.
New York City Wetware iGEM Team
Extraordinary Biology NumbersExtremophile Numbers
Extraordinary Synthetic Biology Numbers
130 Celsius 130 MPa
1.08-mega-base pair 4 Days
Deinococcus radiodurans (D. rad)
One to two hours of acute six to ten Gray radiation exposure.
12,000 Gray!!!!!
Life of the Cell
A Bad Day
Base Damage Single Strand Break Double Strand Break
Active Site Damage
The Extraordinary Weapon (Kryptonite)
1. H2O H· + OH·
2. OH· + OH· H2O2
3. Fe2+ + H2O2 → Fe3+ + OH· + OH−
Generation of Hydroxyl Radical via the Fenton Reaction
What Makes D. rad So Special?
1. Cellular Manganese-Iron Ratios
2. Neutralization of Superoxide Radicals
3. Superior DNA Repair
O-Mn Fe
Three Basic Approaches
What Makes D. rad So Special?
1. Cellular Manganese-Iron Ratios Mn Fe
Three Basic Approaches
What Makes D. rad So Special?
2. Neutralization of Superoxide Radicals
O-Three Basic Approaches
What Makes D. rad So Special?
3. Superior DNA Repair
Three Basic Approaches
recA Repair Protein
Making E. coli Radioresistant
Mn/Fe O-
DNA Repair
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Future Applications
Waste Hungry Bacteria MegaBrick Clean Earth
Bioremediation
Future Applications
MegaBrickStem Cells Sick Person Healthy Person
Medical Applications
in vitro meat and moral traditions
Kosher Vegetarian Vegan
Legal Precedent: Gelatin
Legal Precedent: Rennet
Moral Considerations
• Ought a vegan or vegetarian to eat meat?• Ought a kosher-observer to eat pork?• Does cultivating meat without encountering
an animal desensitize us as eaters? Does it undermine the relationship between ourselves and animals?
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