rainbow electrophoresis technique used to identify individuals by their dna profiles dna...
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RainbowElectrophoresis
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TECHNIQUE USED TO IDENTIFY INDIVIDUALS BY THEIR DNA PROFILES
DNA Fingerprinting
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The Importance of DNA Fingerprinting
PaternityTests
MedicalDiagnosis
Crime SceneInvestigation& Forensics
ForensicAnthropology
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Learning Objectives
At the end of this lesson, you will be able to…
(1)Come up with ideas of how DNA fingerprinting can be applied in different areas
(2)Perform a gel electrophoresis experiment
(3)Define PCR and DNA gel electrophoresis
(4)Predict the results of the gel electrophoresis experiment according to different hypotheses
(5)Determine the answer to a case study problem by analyzing gel electrophoresis results
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What are…DNA
Genes
Sequence
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Polymorphic DNA Regions
Some regions of the human chromosome are polymorphic or dimorphic
e.g. Dimorphic TPA (Tissue Plasminogen Activator) gene: Some TPA alleles possess Alu elements.
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Polymerase Chain Reaction
PCR (Polymerase Chain Reaction): an enzymatic amplification process
Videohttp://www.sumanasinc.com/webcontent/
animations/content/pcr.html
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Gel Electrophoresis
Gel Electrophoresis:process in which molecules (e.g. DNA) are separated according to size and electrical charge by applying an electric current to the gel matrix
Videohttp://www.sumanasinc.com/webcontent/
animations/content/gelelectrophoresis.html
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RainbowElectrophoresis
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Questions?
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Related Professions
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What I do: Operate on patients and do research to understand why people get
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Best part of job: I feel privileged that patients trust
us to do our best to help them live longer and better lives.
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