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Salvador, Pamela Tan, Bernadette

Simbe, Irvinne Torno, Benedict

Superable, Aries Villadolid, Carla

Zaballero, Cesar

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Flaviviridae virus

Flavi-like virus

Route of transmission:PARENTERAL

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Incubation periodis about 8 weeks

Anorexia

Resembles otherhepatitis virus

Nausea

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FatigueLoss of

appetite

FeverYellowing of

the skin(jaundice)

CirrhosisHepatocellular

carcinoma

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Test sample (serum)

Detects: anti-HCV

The colored HCV specificrecombinant antigen-

colloidal gold conjugate

complexes with HCVspecific antibodies in thesample

Complex = immobilizedby the HCV specific

recombinant antigenscoated on the membrane

leading to formation of acolored band whichconfirms a positive result.

(Test region)

The unreacted conjugateand unbound complex of

move further on themembrane and are

subsequently

immobilized by the anti-rabbit antibodies coatedon the membrane at thecontrol region. Forming

a colored band.

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SD BIOLINE HCV kit

Diluent

Micropipet

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Appear at the left section of the result windowto show that the test is working properly

CONTROLBAND

Appear at the right section of the result

window that indicates the test resultTEST BAND

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Negative: The presence of only one purple color band

within the Results Window

Positive: The presence of two color bands (T andC) within the Results Window

Invalid: Neither the test band nor the control band

appears

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In our experiment

Only one colored band appeared in the

control region C Thus, the result is

negative.

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