rapid turnover in micro- and macrobiome of free-ranging primate
TRANSCRIPT
ECM7 – Stockholm, Sweden, 21.8.2015
Tuomas Aivelo
University of Helsinki
[email protected]@aivelo
Rapid turnover in micro- and macrobiome of free-ranging primate
Microbiome research is trending
There's lack of studies connecting microbiome and other intestinal
organisms
Longitudinal series of parasites and microbiome very rare
Longitudinal study How helminth and
microbiome communities differ in dynamics?
How intestinal parasites affect microbiome?
Do microbiome affect intestinal microbiome infection probability?
Study system Rufous mouse lemur
(Microcebus rufus) Long-lived, small-sized, easy to
handle Eastern Madagascar Individuals followed for 2
(microbiome) or 3 (parasites) years
Fecal sampling: 16S sequencing microbiome, 18S sequencing nematodes, other parasites morphologically
Medlar et al. 2014 BMC Evolutionary Biology
Nematodes
Component community stable, but infracommunity variable
Aivelo et al. 2015 Parasitology
Nematodes
Component community stable, but infracommunity variable
Aivelo et al. 2015 Parasitology
Significant variables
- Infracommunity: - All putative species uniformly distributed over hosts- No effect of previous infection
- Component community: - Prevalence higher in 2011 than in 2012- No effect of sex or site
Aivelo et al. 2015 Parasitology Rafalinirna et al. In press Madagascar Conservation and Development
Significant variables
- Infracommunity: - All putative species uniformly distributed over hosts- No effect of previous infection
- Component community: - Prevalence higher in 2011 than in 2012- No effect of sex or site
Aivelo et al. 2015 Parasitology Rafalinirna et al. In press Madagascar Conservation and Development
Microbiome
On population level stable, in individual level highly variable
20122013
Aivelo et al. in prep
Microbiome
On population level stable, in individual level highly variable
2012
Aivelo et al. in prep
Microbiome
On population level stable, in individual level highly variable
2013Aivelo et al. in prep
Microbiome
On population level stable, in individual level highly variable
2013Aivelo et al. in prep
Significant variables
Aivelo et al. in prep
Parasite presence
Does not affect microbiome richness or diversity
EXCEPT:
20
40
60
No Eimeria EimeriaAivelo et al. in prep
Work under progress: Interactions
Aivelo et al. in prep
Why mouse lemurs are different?
Heterothermy? Small-size? Variation in food?
In nutshell
Both microbiome and macrobiome vary wildly in mouse lemurs
There seems to be interactions, but...
Random
Repeatability of 0.332
Aivelo et al. 2015 Parasitology