rapid’salmonella agar short protocol - bio-rad...355-3834 oxidase disks, 2 x 50 disks 356-0781...
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RAPID’Salmonella Agar Short Protocol
RAPID’Chromogenic Methods • Food Testing
■■ Quick response Detection of Salmonella spp. in less than 42 hours■■ Easy protocol Only 1 single broth and 1 single solid agar plate ■■ Easy confi rmation Salmonella Latex■■ Meets the regulations Suitable as second medium in the ISO 6579 standard Detects Salmonella Typhi/Paratyphi, lactose-positive, motile and non-motile Salmonella ■■ Validations Certifi ed NF VALIDATION according to the ISO 16140 standard Validated by the AOAC Research Institute NordVal approved
For more informations: www.foodscience.bio-rad.com
The Difference is Clear. RAPID’Chromogenic Range for Food and Environmental Testing
RAPID’Salmonella Agar
PrincipleThe principle behind RAPID’Salmonella relies on the demonstration of two enzymatic activities. Salmonella spp. takes the form of typical, readily identifi ed magenta colonies (detection of C8 esterase). Counter selection is used to reveal other bacteria with a different color.
RAPID’Salmonella Short Protocol: Easy and Flexibility of Use!
Detection of Salmonella spp.
Certifi ed NF VALIDATION according to ISO 16140Validated by the AOAC Research Institute
NordVal approved
The concentrated solution, once reconstituted,can be stored for 1 week at RT or at +2-8°C.
After incubation, the enrichment broth may bestored at +2-8°C for 72 hours, before
inoculating RAPID’Salmonella.
1
2
or
Reading & confirmation
Salmonella Latex test or Oxidase/OMNI-O/ONPG
or iQ-Check™ Salmonella
1 RAPID’Salmonellacapsule qsp 2.5 l diluted
in 10 ml of BPWor distilled water
or
1 box of RAPID’Salmonellasupplement
qsp 100 analysis
1 RAPID’Salmonellacapsule qsp 250 mlfor 25 g of sample
Plating-out
η g sample + 9 x η ml BPW
Quality indicators enumeration
η x 0.04 mlof concentrated
solution
Selectiveenrichment
Selectiveisolation
RAPID’Salmonella
Stock solution
41.5°C ± 1°C18 hrs ± 2 hrs
37°C ± 1°C24 hrs ± 2 hrs
2 2 1
Dilution Table for RAPID’Salmonella Capsule qsp 2.5 l
Latex Confi rmation: Easy and Fast Confi rmation Step!Salmonella Latex and Salmonella Confi rm Latex are based on agglutination of Salmonella spp. by specifi c antisera. Salmonella Latex allows the detection of Salmonella groups B to E and G, and Salmonella Confi rm Latex allows the detection of Salmonella spp.
1 2 3 4
After enrichment, isolate presumptive Salmonella
colonies on a solid medium
Deposit 1 free falling drop of latex onto a circle of an agglutination card and add
1 colony of presumptive Salmonella
Mix reagents thoroughly Rock the slide in a circular motion for 30 seconds
and observe for agglutination
No agglutination = negative result
Agglutination = positive result
Easy to Interpret
EnrichmentPlate
incubation
Primaryenrichment
2 Secondary enrichments
4 Selective Biochemicaltests
Results
RResult
Sample in g (=) BPW in ml (= 9 x η) Salmonella capsule solution in ml (= η x 0.04)
1 9 0.04 2 18 0.08 3 27 0.12 4 36 0.16 10 90 0.4 25 225 1 50 450 2 100 900 4
RAPID’Salmonella Short Protocol
Standard Reference Methods agar plates
Fast results■■ Less than 42 hours for results to detect Salmonella spp.■■ Fast confirmation (less than 1 minute)
One broth + one plate■■ Saves money■■ Saves handling time
BPW enrichment can be used for sample dilutions■■ No need for another stock solution (such as tryptone salt)■■ Several parameters could be checked from the same bag
Easy to use■■ Solid agar plates■■ Easy confirmation
Flexibility of use■■ RAPID’Salmonella capsule qsp 250 ml: standardized format
for 25 g of food■■ RAPID’Salmonella capsule qsp 2.5 l: adapted for high
throughput laboratory and all sample weight
Very low false positive rate (<1% all matrices*)■■ Saves on useless confirmations
Meets regulatory expectations■■ Can be used as second medium in the ISO 6579 standard■■ RAPID’Salmonella detects motile and non-motile Salmonella,
as well as lactose-positive Salmonella, Salmonella Typhi and Salmonella Paratyphi
* during ISO 16140 accuracy tests
Key Benefits
Ordering Information
Catalog # Description
Buffered Peptone Water/BrothDehydrated356-4684 500 g
Ready to use355-4179 225 ml x 6 bottles355-5795 3 l x 4 bags355-5790 5 l x 2 bags
Enrichment Supplement356-4709 RAPID’Salmonella Capsule, 10 x conc, 100 capsules (1 capsule qsp 2.5 l)356-4710 RAPID’Salmonella Capsule, 100 capsules (1 capsule qsp 250 ml)356-4712 RAPID’Salmonella Supplement, 1 box (qsp 100 analysis)
Catalog # Description
RAPID’Salmonella/AgarReady to use356-3961 90 mm x 20 dishes356-3963 90 mm x 120 dishes
Dehydrated356-4705 500 g
Confirmation355-3834 Oxidase disks, 2 x 50 disks356-0781 Omni-O (A-60), 3 ml355-3822 ONPG, 50 disks355-6710 Salmonella Latex kit, 75 tests355-6711 Salmonella Confirm Latex, 50 tests
iQ-Check™ Salmonella II/Kit357-8123 96 tests
RAPID’Salmonella Salmonella spp. Other Enterobacteriaceae
© Bio-Rad 16447 Rev D 05/2013
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