r&d systems neuroscience reference guide for research use only. not for use in diagnostic...

NEUROSCIENCE REFERENCE GUIDE 2007

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Alphabetical Listing of Neuroscience-Related Molecules:

NEURAL DEVELOPMENT ......................................................... 2-15Bone Morphogenetic Protein Family & Related Molecules ....................................2EGF Family ...............................................................................................4Ephs/Ephrins ............................................................................................4FGF Family ...............................................................................................6GDNF Family .............................................................................................6Hedgehog Family .......................................................................................6IGF Family ................................................................................................6Netrins & Receptors ....................................................................................8Neurotrophic Factors, Receptors, & Related Molecules ...................................8, 10Notch Family & Related Molecules................................................................10ROBO/Slit...............................................................................................10Semaphorin Family & Related Molecules .......................................................12SLITRK Family .........................................................................................12VEGF/PDGF Family ...................................................................................12Wnt-Related Molecules .............................................................................14Adhesion & Other Axon Guidance Molecules ...................................................14

NEURAL STEM CELL & DIFFERENTIATION ........................ 16-21

NEURAL TRANSMISSION ...................................................... 22-27Neurotransmitters, Neuroactive Molecules, & Associated Enzymes .......................22Neurotransmitter Receptors, Ion Channels, & Other Transmembrane Proteins .........24Synapse-Related Molecules ........................................................................26Signal Transduction ..................................................................................26

NEURODEGENERATIVE DISEASE & NEUROINFLAMMATION ........................................................ 28-36Neurodegenerative Disease ..................................................................28, 30Neuroinflammation ............................................................................32, 34

R&D Systems Product Lines ........................................................21, 27, 37-39

Reference selection was not based on exclusive use of R&D Systems products or scientific merit. Rather, references were selected randomly or based on the use of unique sample types or applications that have not been validated by R&D Systems.

R&D Systems NEUROSCIENCE REFERENCE GUIDER&D Systems offers an array of tools to study many aspects of nervous system function ranging from developmental processes such as axon guidance and pattern formation to mechanisms underlying the aging process and neurodegenerative disease. Our product lines include high quality antibodies, proteins, assay kits, and cell culture reagents to enable a range of experimental applications for a variety of sample preparations.

We know that you rely on primary literature to aid in the selection of reagents for your neuroscience research. R&D Systems maintains a reference database that contains published peer-reviewed scientific journal articles citing the use of our products. Many of these studies incorporate unique sample types or applications, demonstrating the broad scope and specificity of R&D Systems reagents. We have thousands of references in this database and more are added monthly.

The Neuroscience Reference Guide highlights a portion of our database specifically devoted to neuroscience-related experimental studies. Within each research area, this guide presents our products in alphabetical order by analyte accompanied by selected references from our database and the sample type(s) tested within each reference. If you do not see a reference for your analyte or application, please contact Technical Service @ (800) 343-7475. We would be happy to check if there is an applicable reference for your needs.

It is our hope that this guide serves as a resource as you engage in your nervous system discoveries.

� For research use only. Not for use in diagnostic procedures.


Activin RIIBApplication: Bioassay - blocking TGF-b signaling effectsProduct: Recombinant Mouse Activin RIIB/Fc Chimeric Protein Catalog # 3725-RBReference(s):Close, J.L. et al. (2005) Retinal neurons regulate proliferation of postnatal progenitors and Müller glia in the rat retina via TGF b signaling. Development 132:3015.Sample Type: rat retinal cells

AmphiregulinApplication: Bioassay - axon outgrowth and cell migrationProduct: Recombinant Mouse Amphiregulin Protein Catalog # 989-ARReference(s):Nilsson, A. et al. (2005) Early changes in gene expression in the dorsal root ganglia after transec-tion of the sciatic nerve; effects of amphiregulin and PAI-1 on regeneration. Brain Res. Mol. Brain Res.136:65.Sample Type: mouse dorsal root ganglia

BDNFApplication: Bioassay - cell survivalProduct: Recombinant Human BDNF Protein Catalog # 248-BDReference(s):Ugolini, G. et al. (2003) Fas/tumor necrosis factor receptor death signaling is required for axotomy-induced death of motoneurons in vivo. J. Neurosci. 23:8526.Sample Type: mouse motor neurons

Application: ELISA KitProduct: Human BDNF Quantikine ELISA Kit Catalog # DBD00Reference(s):Mahmood, A. et al. (2004) Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury. J. Neurotrauma 21:33.Sample Type: rat brain homogenate

NEURAL DEVELOPMENTMOLECULE MONOCLONAL

ANTIBODIESPOLYCLONALANTIBODIES

CONJUGATEDANTIBODIES

RECOMBINANT&NATURALPROTEINS

ELISA/ASSAYS

BoneMorphogeneticProteinFamily&RelatedMolecules

ActivinRIA/ALK-�* H H H H

ActivinRIIA H H H H

ActivinRIIB H H H HM

BAMBI/NMA HM H

BMP-� HZ H H HZ HMR

BMP-3 H H H H

BMP-3b/GDF-�0 H H

BMP-4 HZ H H HZ H

BMP-5 H H H H H

BMP-6 H H H H H

BMP-7 H H H H H

BMP-8 H H H H

BMP-9 H H H H

BMP-�0 H H

BMP-�5/GDF-9B H H H

BMPR-IA/ALK-3 H H H HM

BMPR-IB/ALK-6 HM H H HM

BMPR-II H H H H

Caronte Ch Ch Ch Ch

Cerberus HM HM HM

Chordin M M M M M

Chordin-Like� H H H H

Chordin-Like� HM HM HM M

COCO HM M M H

CRIM� H H H

Cripto HM HM HM HM H

Crossveinless-� HM HM HM HM

Cryptic HM HM HM H

DAN HM HM HM HM H

Gremlin M M M

Nodal M M HM

Noggin M M HM

PRDC M M M M

SKI H

Smad7* HMR H

SOST HM HM HM HM

TSG M M M M M

Key: H Human M Mouse R Rat B Bovine Ca Canine Ch Chicken CR Cotton Rat D Drosophila E Equine F Feline Ms Multi Species P Porcine Pr Primate Rb Rabbit Tg T. gondii V Viral X Xenopus Z Zebrafish

*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/TGFBetaReceptorsforcompletelisting.


USA & Canada (800) 343-7475 | UK & Europe +44 (0)1235 529449 | China (21) 52380373 | www.RnDSystems.com 3

BDNF, continuedApplication: ImmunohistochemistryProduct: Human BDNF Polyclonal Antibody Catalog # AF248Reference(s):Ichikawa, H. et al. (2006) Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei. Brain Res. 1081:113.Sample Type: rat trigeminal ganglia

BDNF Expression in Rat Spinal Cord. BDNF was detected in cryostat sections of rat spinal cord tissue sections using anti-human BDNF polyclonal antibody (Catalog # AF248). Tissue was stained using Rhodamine RedTM X-conjugated goat anti-chicken secondary antibody and coun-terstained with FluoroNisslTM Green.

Application: Western BlotProduct: Human BDNF Monoclonal Antibody Catalog # MAB248Reference(s):Peiris, T.S. et al. (2004) Brain-derived neurotrophic factor mRNA and protein in the piglet brainstem and effects of intermittent hypercapnic hypoxia. Brain Res. 1029:11.Sample Type: porcine cortex, hippocampus, and brain stem homogenates

BMP-2Application: Bioassay- differentiation Product: Recombinant Human BMP-2 Protein Catalog # 355-BMReference(s):Hsieh, J. et al. (2004) IGF-I instructs multipotent adult neural progenitor cells to become oligodendrocytes. J. Cell Biol. 164:111.Sample Type: rat neural progenitor cells

Application: Bioassay - SMAD PhosphorylationProduct: Recombinant Human BMP-2 Protein Catalog # 355-BMReference(s):Panchision, D.M. et al. (2007) Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24. Stem Cells 25:1560.Sample Type: mouse cortical cells

BMP-4Application: Bioassay - proliferationProduct: Recombinant Human BMP-4 Protein Catalog # 314-BPReference(s):Sim, F.J. et al. (2006) Complementary patterns of gene expression by human oligodendrocyte progenitors and their environment predict determinants of progenitor maintenance and differentiation. Ann. Neurol. 59:763.Sample Type: human oligodendrocyte progenitor cells

BMP-7Application: Bioassay - signaling effectsProduct: Recombinant Human BMP-7 Protein Catalog # 354-BPReference(s):Vallstedt, A. et al. (2005) Multiple dorsoventral origins of oligodendrocyte generation in the spinal cord and hindbrain. Neuron 45:55.Sample Type: mouse spinal cord and hindbrain explants

Application: Bioassay - differentiationProduct: Recombinant Human BMP-7 Protein Catalog # 354-BPReference(s):Salero, E. & M.E. Hatten (2007) Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. USA 104:2997.Sample Type: mouse embryonic stem cells

BMPR-IA/ALK-3Application: Flow CytometryProduct: Human BMPR-IA/ALK-3 Polyclonal Antibody Catalog # AF346Reference(s):Panchision, D.M. et al. (2007) Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24. Stem Cells 25:1560.Sample Type: mouse cortical cells

Application: ImmunohistochemistryProduct: Human BMPR-IA/ALK-3 Polyclonal Antibody Catalog # AF346Reference(s):Wang, P.Y. et al. (2005) Müllerian inhibiting substance acts as a motor neuron survival factor in vitro. Proc. Natl. Acad. Sci. USA 102:16421.Sample Type: mouse lumbar spinal cord, ovaries, and testes

E-CadherinApplication: Bioassay - neurite outgrowthProduct: Recombinant Human E-Cadherin/Fc Chimeric Protein Catalog # 648-ECReference(s):Oblander, S.A. et al. (2007) E-cadherin promotes retinal ganglion cell neurite outgrowth in a protein tyrosine phosphatase-m-dependent manner. Mol. Cell. Neurosci. 34:481.Sample Type: chicken retinal explant

ChordinApplication: Bioassay - blocking BMP signaling effectsProduct: Recombinant Mouse Chordin Protein Catalog # 758-CNReference(s):Vallstedt, A. et al. (2005) Multiple dorsoventral origins of oligodendrocyte generation in the spinal cord and hindbrain. Neuron 45:55.Sample Type: mouse spinal cord and hindbrain explants

Rhodamine Red and FluoroNissl are trademarks of Molecular Probes, Inc.

4 For research use only. Not for use in diagnostic procedures.


NEURAL DEVELOPMENT DCCApplication: ImmunohistochemistryProduct: Mouse DCC Polyclonal Antibody Catalog # AF844Reference(s):Tsai, H.H. et al. (2003) Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal. Develop-ment 130:2095.Sample Type: rat and chicken oligodendrocyte precursors

Application: NeutralizationProduct: Mouse DCC Polyclonal Antibody Catalog # AF844Reference(s):Tsai, H.H. et al. (2003) Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal. Develop-ment 130:2095.Sample Type: chicken spinal cord explant

DLL4Application: In Vivo & Bioassay - differentiationProduct: Recombinant Human DLL4 Protein Catalog # 1506-D4Reference(s):Aranguren, X.L. et al. (2007) In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells. Blood 109:2634.Sample Type: mouse & mouse multipotent adult progenitor cells

EGFApplication: Bioassay - neurosphere assayProduct: Recombinant Human EGF Protein Catalog # 236-EGReference(s):Wang, L. et al. (2004) Erythropoietin up regulates SOCS2 in neuronal progenitor cells derived from SVZ of adult rat. Neuroreport 15:1225.Sample Type: rat neural progenitor cells

EphA3Application: Western BlotProduct: Mouse EphA3 Polyclonal Antibody Catalog # AF640Reference(s):Vaidya, A. et al. (2003) EphA3 null mutants do not demonstrate motor axon guidance defects. Mol. Cell. Biol. 23:8092.Sample Type: mouse spinal cord homogenate

MOLECULE MONOCLONALANTIBODIES

POLYCLONALANTIBODIES



ELISA/ASSAYS

EGFFamily

Amphiregulin HM HM HM HM HM

Betacellulin HM HM HM HM H

EGF HM HMR HM HMR HM

HB-EGF H H H H H

LRIG� M M

LRIG3 H H

Neuregulin-�/NRG� H H H H H

Neuregulin-3/NRG3 M M

TGF-a H H H H

TMEFF�/Tomoregulin-�

H HM M

TMEFF� H H

EGFR HM HM HM HM H

Erb�/Her� H H H H H

Erb3 H H H H H

Erb4 H H H H H

Herstatin H

Ephs/Ephrins

EphA� H HM H HM

EphA� HM HM HM HM

EphA3 M M M M

EphA4 M M M M

EphA5 R MR R HMR

EphA6 M M M

EphA7 M M M M

EphA8 M M M M

EphB� R R R R

EphB� M M M

EphB3 M M M

EphB4 HM HM M HM

EphB6 M M M HM

Ephrin-A� M M M M

Ephrin-A� M M M M

Ephrin-A3 H H H H

Ephrin-A4 HM HM HM HM

Ephrin-A5 H H

Ephrin-B HMRChX

Ephrin-B� M M M M

Ephrin-B� MZ MZ MZ

Ephrin-B3 H H H H




EphA4Application: ImmunohistochemistryProduct: Mouse EphA4 Polyclonal Antibody Catalog # AF641Reference(s):Fabes, J. et al. (2006) Accumulation of the inhibitory receptor EphA4 may prevent regeneration of corticospinal tract axons following lesion. Eur. J. Neurosci. 23:1721.Sample Type: mouse brain and spinal cord

EphA7Application: Immunohistochemistry & Bioassay - retinal stripe assayProduct: Recombinant Mouse EphA7/Fc Chimeric Protein Catalog # 608-A7Reference(s):Rashid, T. et al. (2005) Opposing gradients of ephrin-As and EphA7 in the superior colliculus are essential for topographic mapping in the mammalian visual system. Neuron 47:57.Sample Type: mouse retinal explant & chicken retinal axons

EphB2Application: ImmunohistochemistryProduct: Mouse EphB2 Polyclonal Antibody Catalog # AF467Reference(s):Bundesen, L.Q. et al. (2003) Ephrin-B2 and EphB2 regulation of astrocyte-meningeal fibroblast interactions in response to spinal cord lesions in adult rats. J. Neurosci. 23:7789.Sample Type: rat spinal cord

Application: ImmunoprecipitationProduct: Mouse EphB2 Polyclonal Antibody Catalog # AF467Reference(s):Nakada, M. et al. (2005) EphB2/R-Ras signaling regulates glioma cell adhesion, growth, and inva-sion. Am. J. Pathol. 167:565.Sample Type: human glioma U87, U251, and T98G glioma cell line lysates

Application: Western BlotProduct: Mouse EphB2 Polyclonal Antibody Catalog # AF467Reference(s):Bundesen, L.Q. et al. (2003) Ephrin-B2 and EphB2 regulation of astrocyte-meningeal fibroblast interactions in response to spinal cord lesions in adult rats. J. Neurosci. 23:7789.Sample Type: rat spinal cord homogenate

EphB3Application: Western BlotProduct: Mouse EphB3 Polyclonal Antibody Catalog # AF432Reference(s):Liu, X. et al. (2006) EphB3: an endogenous mediator of adult axonal plasticity and regrowth after CNS injury. J. Neurosci. 26:3087.Sample Type: mouse optic nerve and retina homogenate

Ephrin-A1Application: Bioassay - growth cone collapseProduct: Recombinant Mouse Ephrin-A1/Fc Chimeric Protein Catalog # 602-A1Reference(s):Egea, J. et al. (2005) Regulation of EphA4 kinase activity is required for a subset of axon guidance decisions suggesting a key role for receptor clustering in Eph function. Neuron 47:515.Sample Type: mouse neurons

Application: Bioassay - dendritic spine retractionProduct: Recombinant Mouse Ephrin-A1/Fc Chimeric Protein Catalog # 602-A1Reference(s):Fu, W.Y. et al. (2007) Cdk5 regulates EphA4-mediated dendritic spine retraction through an ephexin1-dependent mechanism. Nat. Neurosci. 10:67.Sample Type: mouse hippocampal slice

Ephrin-A2Application: Immunohistochemistry

Ephrin-A2 Expression in Rat Embryonic Neurons. Ephrin-A2 was detected in rat embryonic hippocampal neurons using goat anti-mouse Ephrin-A2 polyclonal antibody (Catalog # AF603; green). Astrocytes were stained using an anti-GFAP antibody (red).

Ephrin-A3Application: Bioassay - dendritic spine outgrowthProduct: Recombinant Human Ephrin-A3/Fc Chimeric Protein Catalog # 359-EAReference(s):Murai, K.K. et al. (2003) Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling. Nat. Neurosci. 6:153.Sample Type: mouse hippocampal slice

Ephrin-A5Application: Bioassay - inhibition of transsynaptic interactionsProduct: Recombinant Human Ephrin-A5/Fc Chimeric Protein Catalog # 374-EAReference(s):Regalado, M.P. et al. (2006) Transsynaptic signaling by postsynaptic synapse-associated protein 97. J. Neurosci. 26:2343.Sample Type: rat hippocampal neurons



NEURAL DEVELOPMENT Ephrin-B1Application: Bioassay - decreased cell growthProduct: Recombinant Mouse Ephrin-B1/Fc Chimeric Protein Catalog # 473-EBReference(s):Nakada, M. et al. (2005) EphB2/R-Ras signaling regulates glioma cell adhesion, growth, and inva-sion. Am. J. Pathol. 167:565.Sample Type: human U87, U251, and T98G glioma cell lines

Application: ImmunohistochemistryProduct: Recombinant Mouse Ephrin-B1/Fc Chimeric Protein Catalog # 473-EBReference(s):Wang, Y. et al. (2005) Induction of ephrin-B1 and EphB receptors during denervation-induced plasticity in the adult mouse hippocampus. Eur. J. Neurosci. 21:2336.Sample Type: mouse brain

Ephrin-B2Application: Bioassay - neurite outgrowthProduct: Recombinant Mouse Ephrin-B2/Fc Chimeric Protein Catalog # 496-EBReference(s):Calo, L. et al. (2005) Nurr1 co-localizes with EphB1 receptors in the developing ventral midbrain, and its expression is enhanced by the EphB1 ligand, ephrinB2. J. Neurochem. 92:235.Sample Type: mouse midbrain/hindbrain boundary explants

Application: ImmunohistochemistryProduct: Mouse Ephrin-B2 Polyclonal Antibody Catalog # AF496Reference(s):Fabes, J. et al. (2006) Accumulation of the inhibitory receptor EphA4 may prevent regeneration of corticospinal tract axons following lesion. Eur. J. Neurosci. 23:1721.Sample Type: mouse brain and spinal cord

Ephrin-B3Application: Bioassay - growth cone collapseProduct: Recombinant Human Ephrin-B3/Fc Chimeric Protein Catalog # 395-EBReference(s):Egea, J. et al. (2005) Regulation of EphA4 kinase activity is required for a subset of axon guidance decisions suggesting a key role for receptor clustering in Eph function. Neuron 47:515.Sample Type: mouse neurons

Application: Western BlotProduct: Human Ephrin-B3 Polyclonal Antibody Catalog # AF395Reference(s):Liu, X. et al. (2006) EphB3: an endogenous mediator of adult axonal plasticity and regrowth after CNS injury. J. Neurosci. 26:3087.Sample Type: mouse optic nerve and retina homogenates





ELISA/ASSAYS

FGFFamily

FGFacidic H HB H HB H

FGFbasic H HB H HMRB H

FGF-4* H H H H H

FGF-8* HM M M M

FGF-BP HR HR HR R

FGFR�,�,3,4,5* (seepage�6fordetails)

Growth/DifferentiationFactorFamily(seewebsitefordetails)

GDNFFamily

Artemin HM HM HM HM

GDNF H H H HR H

Neurturin H HM HM HM

Persephin HM HM H HM

Gas� H HM HM HM M

GFRa-�/GDNFRa-� R HR HR HR

GFRa-�/GDNFRa-� H HM HM HM

GFRa-3/GDNFRa-3 H HM HM H

GFRa-4/GDNFRa-4 HM HM HM

Ret HM HM HM HM

HedgehogFamily

DesertHedgehog M M M

IndianHedgehog M M M

SonicHedgehog HM M HM HM M

BOC HM HM HM

CDO M M M

DISP� H

GLI-�,3 HM

GLI-� HM H

GSK-3a* H HMR

Hip M M M M M

Patched M M

Patched� H

IGFFamily

IGF-I H H H H H

IGF-II HM HM HM H M

IGF-IR/CD�� H H H H H

IGFBP4* H H H H H

IGFBP-L� H H


*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/FGFFamilyforcompletelisting.



ErbB4Application: Bioassay - blocking NRG1 signalingProduct: Recombinant Human ErbB4/Fc Chimeric Protein Catalog # 1131-ERReference(s):Wang, Z. et al. (2007) Contrasting effects of mitogenic growth factors on myelination in neuron-oligodendrocyte co-cultures. Glia 55:537.Sample Type: rat dorsal root ganglia and oligodendrocyte precursor cell co-culture

FGF basicApplication: Bioassay - differentiationProduct: Recombinant Human FGF basic Protein Catalog # 233-FBReference(s):Salero, E. & M.E. Hatten (2007) Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. USA 104:2997.Sample Type: mouse embryonic stem cells

Yamashita, H. et al. (2006) Embryonic stem cell-derived neuron models of Parkinson’s disease exhibit delayed neuronal death. J. Neurochem. 98:45.Sample Type: mouse Nurr-1 embryonic stem cells

FGF-4Application: Bioassay - differentiationProduct: Recombinant Human FGF-4 Protein Catalog # 235-F4Reference(s):Salero, E. & M.E. Hatten (2007) Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. USA 104:2997.Sample Type: mouse embryonic stem cells

FGF-8Application: Bioassay - neurite extension and directional outgrowthProduct: Recombinant Mouse FGF-8b Protein Catalog # 423-F8Reference(s):Gill, J.C. & P.S. Tsai (2006) Expression of a dominant negative FGF receptor in developing GNRH1 neurons disrupts axon outgrowth and targeting to the median eminence. Biol. Reprod. 74:463.Sample Type: mouse neurons

Application: Bioassay - neuronal differentiationProduct: Recombinant Mouse FGF-8b Protein Catalog # 423-F8Reference(s):Yamashita, H. et al. (2006) Embryonic stem cell-derived neuron models of Parkinson’s disease exhibit delayed neuronal death. J. Neurochem. 98:45.Sample Type: mouse transgenic Nurr-1 embryonic stem cells

F-SpondinApplication: Bioassay – neurite outgrowth

A

B

F-Spondin-induced Neurite Outgrowth. A: Recombinant human F-Spondin (Catalog # 3135-SP), immobilized on a nitrocellulose-coated microplate, promotes neurite outgrowth in chick embryonic (E13) dorsal root ganglion neurons when cultured in the presence of re-combinant human b-NGF (Catalog # 256-GF). B: Neurons on a control plate in the absence of F-Spondin exhibit little neurite outgrowth.

GDF-7Application: Bioassay - differentiationProduct: Recombinant Mouse GDF-7 Protein Catalog # 779-G7Reference(s):Salero, E. & M.E. Hatten (2007) Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. USA 104:2997.Sample Type: mouse embryonic stem cells

GDNFApplication: Bioassay – neuronal survival and antibody blockade

0.1

0.22

0.20

0.24

0.26

0.28

0.30

0.32

0.1

Neu

ron

al S

urv

ival

(MTT

O.D

.)

Recombinant GDNF (ng/mL)

GDNF Antibody (µg/mL)0.01 1

1 10

GDNF-induced Neuronal Survival. Recombinant human GDNF protein (Catalog # 212-GD) increases chick embryonic (E15) dorsal root ganglion neuronal survival in a dose-dependent manner (blue) as measured by the MTT Viability Assay (Catalog # TA5355). The neuronal survival effect elicited by GDNF is neutralized (maroon) by increasing concentrations of goat anti- human GDNF polyclonal antibody (Catalog # AF-212-NA).



NEURAL DEVELOPMENT GDNF, continuedApplication: Immunohistochemistry

Product: Human GDNF Polyclonal Antibody Catalog # AF-212-NAReference(s):Winkler, C. et al. (2006) Continuous exposure to glial cell line-derived neurotrophic factor to mature dopaminergic transplants impairs the graft’s ability to improve spontaneous motor behavior in Parkinsonian rats. Neuroscience 141:521.Sample Type: rat brain

Pineda, J.R. et al. (2007) Neuroprotection by GDNF-secreting stem cells in a Huntington’s disease model: optical neuroimage tracking of brain-grafted cells. Gene Ther. 14:118.Sample Type: mouse brain

Oo, T.F. et al. (2005) Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat. J. Comp. Neurol. 484:57.Sample Type: rat substantia nigra and striatal tissue

Application: Western BlotProduct: Human GDNF Polyclonal Antibody Catalog # AF-212-NAReference(s):Pina Serra, M. et al. (2002) Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood. Brain Res. 928:138.Sample Type: rat and human hippocampal homogenates

GFRa-1/GDNF Ra-1Application: Bioassay - NCAM-mediated cell adhesionProduct: Recombinant Rat GFRa-1/Fc Chimeric Protein Catalog # 560-GRReference(s):Paratcha, G. et al. (2003) The neural cell adhesion molecule NCAM is an alternative signaling recep-tor for GDNF family ligands. Cell 113:867.Sample Type: African green monkey COS-7 kidney fibroblast cells transfected with rat p140NCAM

Application: ImmunohistochemistryProduct: Rat GFRa-1 Polyclonal Antibody Catalog # AF560Reference(s):Kobayashi, T. et al. (2006) Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Stroke 37:2361.Sample Type: rat neurospheres

Cho, J. et al. (2004) Glial cell line-derived neurotrophic factor receptor GFRa1 is expressed in the rat striatum during postnatal development. Brain Res. Mol. Brain Res. 127:96.Sample Type: rat brain

Application: NeutralizationProduct: Rat GFRa-1 Polyclonal Antibody Catalog # AF560Reference(s):Taylor, A.R. et al. (2007) Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival. J. Neurosci. 27:634.Sample Type: chicken motor neurons





ELISA/ASSAYS

Netrins&Receptors

DCC M M M

Neogenin M M M M

Netrin-� MCh MCh MCh MCh

Netrin-� Ch Ch Ch Ch

Netrin-4 H HM HM HM

Netrin-G�a M M M

Netrin-G�a M M

Nope M M M M

UNC5H� R R R

UNC5H� R R R R

UNC5H3 H H H

UNC5H4 H H H

NeurotrophicFactors,Receptors,&RelatedMolecules

BDNF H H H H H

Cardiotrophin-� HM HM HM HM M

CNTF HR HR HR HR HR

CNTFRa H HR HR HR

EN-RAGE H H

gp�30 HM HM HM HM H

LIFRa H H H H

LINGO-� H H

LINGO-� H H

MAG/Siglec-4a R R R R

Meteorin M M M

Midkine H H H

Neuritin H H

Neuropoietin M M M M

b-NGF H HR HR HMR HR

NGFR/p75NTR H HM HM HM

Nogo-A R HR HR

NogoReceptor HM HM HM HM

NgR�/NgRH� H H

NgR3/NgRH� H H H

NRAGE H

NT-3 H H H H H

NT-4 H H H HM H

OMgp HM HM HM HM

Pleiotrophin H H H

SorCS� H HM

Continued on page 10




GFRa-1/GDNF Ra-1, continuedApplication: Western BlotProduct: Rat GFRa-1 Polyclonal Antibody Catalog # AF560Reference(s):Pierchala, B.A. et al. (2006) Glial cell line-derived neurotrophic factor-dependent recruitment of Ret into lipid rafts enhances signaling by partitioning Ret from proteasome-dependent degradation. J. Neurosci. 26:2777.Sample Type: rat sympathetic neuron lysate

HB-EGFApplication: Western BlotProduct: Human HB-EGF Polyclonal Antibody Catalog # AF-259-NAReference(s):Kodama, T. et al. (2004) ADAM12 is selectively overexpressed in human glioblastomas and is associ-ated with glioblastoma cell proliferation and shedding of heparin-binding epidermal growth factor. Am. J. Pathol. 165:1743.Sample Type: human glioblastoma homogenate

HGFApplication: Immunohistochemistry & Western BlotProduct: Human HGF Polyclonal Antibody Catalog # AF-294-NAReference(s):Giacobini, P. et al. (2007) Hepatocyte growth factor acts as a mitogen and guidance signal for gonadotropin hormone-releasing hormone-1 neuronal migration. J. Neurosci. 27:431.Sample Type: mouse brain and mouse brain homogenate

IGFBP-4Application: ELISA KitProduct: Human IGFBP-4 DuoSet Catalog # DY804Reference(s):Moreno, M.J. et al. (2006) Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti- angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)- differentiated glioblastoma cells. Glia 53:845.Sample Type: human U87MG glioma cell line supernatant

IGF-IApplication: ELISA KitProduct: Mouse IGF-I Quantikine ELISA Kit Catalog # MG100Reference(s):Engesser-Cesar, C. et al. (2007) Wheel running and fluoxetine antidepressant treatment have differential effects in the hippocampus and the spinal cord. Neuroscience 144:1033.Sample Type: mouse hippocampal and spinal cord homogenates and serum

IGF-IApplication: ImmunohistochemistryProduct: Mouse IGF-I Polyclonal Antibody Catalog # AF791Reference(s):Schori, H. et al. (2007) Genetic manipulation of CD74 in mouse strains of different backgrounds can result in opposite responses to central nervous system injury. J. Immunol. 178:163.Sample Type: mouse spinal cord

Jagged 1Application: Bioassay - differentiationProduct: Recombinant Rat Jagged 1/Fc Chimeric Protein Catalog # 599-JGReference(s):Aranguren, X.L. et al. (2007) In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells. Blood 109:2634.Sample Type: human multipotent adult progenitor cells

NCAM-L1Application: Bioassay- neural crest migrationProduct: Recombinant Human NCAM-L1/Fc Chimeric Protein Catalog # 777-NCReference(s):Anderson, R.B. et al. (2006) The cell adhesion molecule L1 is required for chain migration of neural crest cells in the developing mouse gut. Gastroenterology 130:1221.Sample Type: mouse embryonic gut explant

Netrin-1Application: Bioassay - effect on axonal growthProduct: Recombinant mouse Netrin-1 Protein Catalog # 1109-N1Reference(s):Lin, L. & O. Isacson (2006) Axonal growth regulation of fetal and embryonic stem cell-derived dopaminergic neurons by Netrin-1 and Slits. Stem Cells 24:2504.Sample Type: mouse embryonic stem cell-derived dopaminergic neurons

�0 For research use only. Not for use in diagnostic procedures.


NEURAL DEVELOPMENT NeurofascinApplication: Bioassay – neurite outgrowth

A

B

Neurofascin-induced Neurite Outgrowth. A: Recombinant rat Neurofascin (Catalog # 3235-NF) immobilized on a microplate promotes neurite outgrowth in rat cortical neurons. B: Corti-cal neurons cultured under the same conditions in the absence of neurofascin exhibit little outgrowth.

Neuropilin-1Application: Immunohistochemistry

Product: Rat Neuropilin-1 Polyclonal Antibody Catalog # AF566Reference(s):Agudo, M. et al. (2005) Regulation of neuropilin-1 by spinal cord injury in adult rats. Mol. Cell. Neurosci. 28:475.Sample Type: rat cervical spinal cord

Neuropilin-1 Expression in Mouse Embryonic Spinal Cord. Neuropilin-1 was detected in a cryostat section of mouse embryonic spinal cord (E15) using anti-rat Neuropilin-1 polyclonal antibody (Catalog # AF566). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).





ELISA/ASSAYS

NeurotrophicFactors,Receptors,&RelatedMoleculescontinued

SorCS3 HM H

Sortilin HM HM HM HM

TrkA HR HMR HR HR H

TrkB HM HM HM HM H

TrkC HM HM HM HM

TROY/TNFRSF�9 M HM HM HM M

NotchFamily&RelatedMolecules

CSL H

DLL� H HR H

DLL3 H

DLL4 M M HM

DNER HM HM HM

HES-� H

HES-4 H H

Jagged� HR HR HR R

Jagged� H H

Notch-� R HR

Notch-� R HR R HR

Notch-3 HM HM HM

Notch-4 H

TACE/ADAM�7 H H H HM H

ROBO/Slit

ROBO� R R R

ROBO� H H H

ROBO3 H H H HM

ROBO4 H H H

Slit3 M



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b-NGFApplication: Bioassay - axonal elongationProduct: Recombinant Human b-NGF Protein Catalog # 256-GFReference(s):Adams, D.N. et al. (2005) Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide. J. Neurobiol. 62:134.Sample Type: chicken dorsal root ganglia

Application: Bioassay - effect on doublecortin expressionProduct: Recombinant Mouse b-NGF Protein Catalog # 1156-NGReference(s):Couillard-Despres, S. et al. (2005) Doublecortin expression levels in adult brain reflect neurogenesis. Eur. J. Neurosci. 21:1.Sample Type: mouse dorsal root ganglia

Application: Bioassay - ERK1/2 signaling activationProduct: Recombinant Rat b-NGF Protein Catalog # 556-NGReference(s):Obata, K. et al. (2004) Activation of extracellular signal-regulated protein kinase in the dorsal root ganglion following inflammation near the nerve cell body. Neuroscience 126:1011.Sample Type: rat L4/5 nerve roots and dorsal root ganglia

NGF R/p75NTR

Application: Bioassay - neurite outgrowthProduct: Recombinant Mouse NGF R/p75NTR/Fc Chimeric Protein Catalog # 1157-NRReference(s):Benson, M.D. et al. (2005) Ephrin-B3 is a myelin-based inhibitor of neurite outgrowth. Proc. Natl. Acad. Sci. USA 102:10694.Sample Type: mouse neurons

NgR2/NgRH1Application: Flow CytometryProduct: Human NgR2/NgRH1 Polyclonal Antibody Catalog # AF2776Reference(s):Fry, E.J. et al. (2007) A role for Nogo receptor in macrophage clearance from injured peripheral nerve. Neuron 53:649.Sample Type: rat sciatic nerve cells

NogginApplication: Bioassay - blocking BMP signaling effectsProduct: Recombinant Mouse Noggin/Fc Chimeric Protein Catalog # 719-NGReference(s):Hsieh, J. et al. (2004) IGF-I instructs multipotent adult neural progenitor cells to become oligodendrocytes. J. Cell Biol. 164:111.Sample Type: rat neural progenitor cells

Vallstedt, A. et al. (2005) Multiple dorsoventral origins of oligodendrocyte generation in the spinal cord and hindbrain. Neuron 45:55.Sample Type: mouse spinal cord and hindbrain explants

Nogo-AApplication: Bioassay - neurite outgrowth

Product: Recombinant Rat Nogo-A/Fc Chimeric Protein Catalog # 2445-NGReference(s):Wright, K.T. et al. (2007) Bone marrow stromal cells stimulate neurite outgrowth over neural pro-teoglycans (CSPG), myelin associated glycoprotein and Nogo-A. Biochem. Biophys. Res. Commun. 354:559.Sample Type: chicken dorsal root ganglia

A

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Nogo-A inhibition of Neurite Outgrowth. A: Embryonic chick dorsal root ganglion (DRG) cells exhibit neurite outgrowth when cultured on nitrocellulose-coated plates treated with laminin. B: Pre-coating similar plates with recombinant rat Nogo-A (Catalog # 2445-NG) completely inhibits neurite outgrowth.

Nogo ReceptorApplication: Western BlotProduct: Human Nogo Receptor Polyclonal Antibody Catalog # AF1208Reference(s):Walmsley, A.R. et al. (2004) Zinc metalloproteinase-mediated cleavage of the human Nogo-66 receptor. J. Cell Sci. 117:4591.Sample Type: Human SH-SY5Y neuroblastoma cell line lysate

Notch-2Application: Immunohistochemistry

Notch-2 Expression in Rat Brain. Notch-2 was detected in cryostat sections of rat brain (through choroid plexus) using anti-rat Notch-2 polyclonal antibody (Catalog # AF1190). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

�� For research use only. Not for use in diagnostic procedures.


NEURAL DEVELOPMENT NT-3Application: Bioassay - differentiationProduct: Recombinant Human NT-3 Protein Catalog # 267-N3Reference(s):Salero, E. & M.E. Hatten (2007) Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. USA 104:2997.Sample Type: mouse embryonic stem cells

Application: ELISA KitProduct: Human NT-3 DuoSet Catalog # DY267Reference(s):Taylor, S.J. et al. (2004) Controlled release of neurotrophin-3 from fibrin gels for spinal cord injury. J. Control. Release 98:281.Sample Type: recombinant human NT-3 protein

PlGFApplication: ELISA KitProduct: Human PlGF Quantikine ELISA Kit Catalog # DPG00Reference(s):Schmidt, N.O. et al. (2005) Brain tumor tropism of transplanted human neural stem cells is induced by vascular endothelial growth factor. Neoplasia 7:623.Sample Type: human brain tumor homogenate

PleiotrophinApplication: ImmunohistochemistryProduct: Human Pleiotrophin Polyclonal Antibody Catalog # AF-252-PBReference(s):Gurok, U. et al. (2004) Gene expression changes in the course of neural progenitor cell differentia-tion. J. Neurosci. 24:5982.Sample Type: mouse brain and neurospheres

Plexin A3Application: Immunohistochemistry

Plexin A3 Expression in Embryonic Spinal Cord. Plexin A3 was detected in mouse embry-onic (E13.5) spinal cord using anti-mouse Plexin A3 polyclonal antibody (Catalog # AF4075). Cells were stained using a NorthernLights-557 fluorochrome-labeled donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).





ELISA/ASSAYS

SemaphorinFamily&RelatedMolecules

CD7� M M M

Integrinb�/CD�9 HM HM H

Neuropilin-� R R R HR

Neuropilin-� HR HR R HR

PlexinA� M

PlexinA3 M

PlexinB� H H

PlexinC� H H

PlexinD� H

Semaphorin3A H H H

Semaphorin3C M M M

Semaphorin3E H H HM

Semaphorin3F M

Semaphorin6A HM HM HM H

Semaphorin6B HM H H H

Semaphorin6C HM HM

Semaphorin6D H H H

Semaphorin7A HM HM HM

TIM-� M M M

SLITRKFamily

SLITRK� H H H

SLITRK� H H H

SLITRK4 H H

SLITRK5 H H H

VEGF/PDGFFamily

PDGF HMs HP

PDGFRb HM HM HM HM H

PDGF-A HR HRMs HR HR HM

PDGF-AB HMs H HR HMR

PDGF-B H HMs H HR HMR

PDGF-C M HM M HM

PDGF-D H H H

PIGF H H H H H

VEGF HMRCaZ HMRCaZ HMRCaZ HMRCaZ HMRCa

VEGFR H

VEGFR�/Flt-� HM HM HM HM HM

VEGFR�/KDR/Flk-� HM HM HM HM HM

VEGFR3/Flt-4 HM HM HM HM HM



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RetApplication: ImmunohistochemistryProduct: Mouse Ret Polyclonal Antibody Catalog # AF482Reference(s):Tamura, S. et al. (2005) TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons. Neuroscience 130:223.Sample Type: mouse dorsal root ganglia

Application: Western BlotProduct: Mouse Ret Monoclonal Antibody Catalog # MAB482Reference(s):Cerchia, L. et al. (2003) The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret. Biochem. J. 372:897.Sample Type: rat PC12 pheochromocytoma cell (transfected with human RET-C634Y) lysate

RGM-BApplication: Immunohistochemistry

RGM-B Expression in Mouse Brain. RGM-B was detected in cryostat sections of mouse brain (trigeminal ganglia) using sheep anti-mouse RGM-B polyclonal antibody (Catalog # AF3597). Tissue was stained using HRP-DAB detection reagents and counterstained with hematoxylin (blue).

ROBO1Application: ImmunohistochemistryProduct: Rat ROBO1 Polyclonal Antibody Catalog # AF1749Reference(s):Wehrle, R. et al. (2005) Expression of netrin-1, slit-1 and slit-3 but not of slit-2 after cerebellar and spinal cord lesions. Eur. J. Neurosci. 22:2134. Sample Type: mouse and rat brain and spinal cord

ROBO4Application: Flow Cytometry

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Detection of ROBO4 by Flow Cytometry. ROBO4 expression was confirmed in transfectants using phycoerythrin(PE)-conjugated mouse anti-human ROBO4 monoclonal antibody (Catalog # FAB2454P, filled histogram). Staining with a PE-conjugated isotype control (Catalog # IC003P; open histogram) highlights the specificity of the ROBO4 antibody.

Semaphorin 3AApplication: Bioassay - growth cone collapseProduct: Recombinant Human Semaphorin 3A/Fc Chimeric Protein Catalog # 1250-S3Reference(s):Gallo, G. (2006) RhoA-kinase coordinates F-actin organization and myosin II activity during sema-phorin-3A-induced axon retraction. J. Cell Sci. 119:3413.Sample Type: chicken dorsal root ganglia

Application: Bioassay - growth cone collapseProduct: Recombinant Human Semaphorin 3A/Fc Chimeric Protein Catalog # 1250-S3Reference(s):Wu, K.Y. et al. (2005) Local translation of RhoA regulates growth cone collapse. Nature: 436:1020.Sample Type: rat dorsal root ganglia

Semaphorin 6BApplication: Bioassay – growth cone collapse

A

B

Growth Cone Collapse by Semaphorin 6B. A: A fully extended dorsal root ganglion (DRG) growth cone growing on a laminin-coated tissue culture plate in the presence of recombinant human b-NGF (Catalog # 256-GF). B: A collapsed DRG growth cone following treatment with recombinant human Semaphorin 6B/Fc (Catalog # 2094-S6).







ELISA/ASSAYS

Wnt-RelatedMolecules

b-Catenin HMR HMRX H

Dishevelled-�* H

Dkk-�* HM HM HM HMR HM

FrizzledFamily (seewebsitefordetails)

Glypican5* HM HM H HM

ICAT H

Kremen-�* HM HM M M M

LRP-6* M H HM

MFRP H HM HM H

Norrin HM H H HM

PRDC M M M M

Pygopus-�* H H

ROR�,ROR� H H H

R-Spondin�* M H

sFRP-3* HM HM HM HM

Soggy-� HM HM HM HM

WIF-� HM HM HM

Wnt-�,3a,4,5a,7a,7b,8a,9a,�0b,��

(seewebsitefordetails)

Wnt-3a,5a,5b,7a HM

Adhesion&OtherAxonGuidanceMolecules

Agrin R R R R

Contactin-�* H H H H

DSCAM H H

DSCAM-L� H

Endocan M HM HM HM

F-Spondin H H

GAP-43 HMR

E-Cadherin* HM HM HM HM H

NCAM-�/CD56* H H H H

Neurofascin R

Reelin M M M

RGM-A HMCh HMCh HMCh HM

RGM-B H HM HM

RGM-C HM

S�00A4 HM

TenascinC HM H H

TenascinR H

TIMP-� HMR HMR HMR HMR HMR

VLDLR M M M

NEURAL DEVELOPMENT Sonic HedgehogApplication: Bioassay - differentiationProduct: Recombinant Human Sonic Hedgehog, N-Terminus (Shh-N) Protein Catalog # 1314-SHReference(s):Aranguren, X.L. et al. (2007) In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells. Blood 109:2634.Sample Type: human multipotent adult progenitor cells

Application: Bioassay - oligodendrogenesisProduct: Recombinant Mouse Sonic Hedgehog, N-Terminus (Shh-N) Protein Catalog # 461-SHReference(s):Miller, R.H. et al. (2004) Patterning of spinal cord oligodendrocyte development by dorsally derived BMP4. J. Neurosci. Res. 76:9.Sample Type: rat spinal cord

Application: ELISA KitProduct: Mouse Sonic Hedgehog (Shh) DuoSet Catalog # DY461Reference(s):Rafuse, V.F. et al. (2005) Neuroprotective properties of cultured neural progenitor cells are associ-ated with the production of sonic hedgehog. Neuroscience 131:899.Sample Type: mouse neural progenitor cell supernatant

Application: In VivoProduct: Recombinant Human Sonic Hedgehog, N-Terminus (Shh-N) Protein Catalog # 1314-SHReference(s):Aranguren, X.L. et al. (2007) In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells. Blood 109:2634.Sample Type: mouse

Tenascin RApplication: Immunohistochemistry

Tenascin Receptor Expression in Human Brainstem. Tenascin R was detected in paraffin-embedded human brainstem (medulla) tissue sections using anti-human Tenascin R polyclo-nal antibody (Catalog # AF3865). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).


*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/Wnt&www.RnDSystems.com/go/CellAdhesionforcompletelisting.



TIMP-1Application: ImmunohistochemistryProduct: Rat TIMP-1 Polyclonal Antibody Catalog # AF580Reference(s):Newton, S.S. et al. (2003) Gene profile of electroconvulsive seizures: induction of neurotrophic and angiogenic factors. J. Neurosci. 23:10841.Sample Type: rat brain

TrkBApplication: Bioassay - inhibition of putative TrkB ligandsProduct: Recombinant Human TrkB Protein Catalog # 397-TRReference(s):Holback, S. et al. (2005) Increased processing of APLP2 and APP with concomitant formation of APP intracellular domains in BDNF and retinoic acid-differentiated human neuroblastoma cells. J. Neurochem. 95:1059.Sample Type: human SH-SY5Y neuroblastoma cell line

Application: In Vivo & Bioassay - blocking of BDNFProduct: Recombinant Human TrkB/Fc Chimeric Protein Catalog # 688-TKReference(s):Rex, C.S. et al. (2007) Brain-derived neurotrophic factor promotes long-term potentiation-related cytoskeletal changes in adult hippocampus. J. Neurosci. 27:3017.Sample Type: rat & rat hippocampal slice

TrkCApplication: Immunohistochemistry & Western BlotProduct: Human TrkC Monoclonal Antibody Catalog # MAB373Reference(s):Wang, J. et al. (2007) Recombinant adenovirus vector-mediated functional expression of neurotro-pin-3 receptor (TrkC) in neural stem cells. Exp. Neurol. 203:123.Sample Type: human neural stem cells & neural stem cell lysate

Application: ImmunohistochemistryProduct: Mouse TrkC Polyclonal Antibody Catalog # AF1404Reference(s):Tamura, S. et al. (2005) TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons. Neuroscience 130:223.Sample Type: mouse dorsal root ganglia

TROY/TNFRSF19Application: Flow CytometryProduct: Human TROY/TNFRSF19 Polyclonal Antibody Catalog # AF1548Reference(s):Fry, E.J. et al. (2007) A role for Nogo receptor in macrophage clearance from injured peripheral nerve. Neuron 53:649.Sample Type: rat sciatic nerve cells

VEGFApplication: ELISA KitProduct: Human VEGF Quantikine ELISA Kit Catalog # DVE00Reference(s):Nakamura, K. et al. (2006) Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1a in neuroblastoma cells. Cancer Res. 66:4249.Sample Type: human neuroblastoma supernatant

VEGF-BApplication: Bioassay - neurogenesisProduct: Recombinant Mouse VEGF-B 186 Protein Catalog # 767-VEReference(s):Sun, Y. et al. (2006) Vascular endothelial growth factor-B (VEGFB) stimulates neurogenesis: evidence from knockout mice and growth factor administration. Dev. Biol. 289:329.Sample Type: mouse embryonic-derived neuronal lineage cells

Application: Western BlotProduct: Mouse VEGF-B 167/186 Polyclonal Antibody Catalog # AF590Reference(s):Sun, Y. et al. (2006) Vascular endothelial growth factor-B (VEGFB) stimulates neurogenesis: evidence from knockout mice and growth factor administration. Dev. Biol. 289:329.Sample Type: mouse brain homogenate

VEGF-CApplication: ImmunohistochemistryProduct: Human VEGF-C Polyclonal Antibody Catalog # AF752Reference(s):Kubo, H. et al. (2002) Blockade of vascular endothelial growth factor receptor-3 signaling inhibits fibroblast growth factor-2-induced lymphangiogenesis in mouse cornea. Proc. Natl. Acad. Sci. USA 99:8868.Sample Type: mouse eye

Wnt-3aApplication: Bioassay - proliferationProduct: Recombinant Mouse Wnt-3a Protein Catalog # 1324-WNReference(s):Inoue, T. et al. (2006) Activation of canonical Wnt pathway promotes proliferation of retinal stem cells derived from adult mouse ciliary margin. Stem Cells 24:95.Sample Type: mouse retinal stem cells



NEURAL STEM CELL & DIFFERENTIATION A2B5Application: ImmunohistochemistryProduct: Human/Mouse/Rat/Chicken A2B5 Monoclonal Antibody Catalog # MAB1416Reference(s):Rosin, C. et al. (2004) Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms. J. Neurochem. 90:1173.Sample Type: human oligodendrocytes

A2B5 Expression in Human Progenitor Cells. A2B5 antigen was detected in human neural progenitor cells using anti-human/mouse/rat/chicken A2B5 monoclonal antibody (Catalog # MAB1416). Cells were stained with a rhodamine-conjugated goat anti-mouse secondary antibody (red) and counterstained with DAPI (blue).

BMI-1Application: Immunohistochemistry

BMI-1 Expression in HeLa Cells. BMI-1 was detected in HeLa cells using anti-human BMI-1 polyclonal antibody (Catalog # AF3334). Cells were stained using a NorthernLights-557 fluoro-chrome-labeled donkey anti-goat IgG secondary antibody (red; Catalog # NL001) and counter-stained with DAPI (blue).

CNDP1Application: Immunohistochemistry

CNDP1 Expression in Neural Progenitor Cells. CNDP1 was detected in differentiated neural progenitor cells using anti-human CNDP1 monoclonal antibody (Catalog # MAB2489). Cells were stained with a rhodamine-conjugated anti-mouse secondary antibody (red) and counter-stained with DAPI (blue).






PRIMERPAIRS

A�B5 HMRCh HMRCh

ABCG� H H

ACE HM HM HM HM

ALCAM HM HM HM HM

BMI-� H H

CarbonicAnhydraseII

H H H H

CD44 H H H

CD90/Thy� H H

CDCP� H H H

Claudin-�� H

Claudin-�� H

CNDP� H H H H

CXCR4* HMF HM HM

FABP6 H

FABP7 H

FGFR� H H

FGFR� HM HM

FGFR3 HM H HM

FGFR4 H M M H

FGFR5 M HM

Frizzled-9* M M

GAD�/GAD67 H H H

GAD�/GAD65 H

Galectin-� M HM HM HM

GFAP H H H HMR

Glut� H

GMF-b H H H

Integrina4/CD49d HM H

Islet-� H

Latexin M HM

LINGO-� H H

*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com.


USA & Canada (800) 343-7475 | UK & Europe +44 (0)1235 529449 | www.RnDSystems.com �7

CXCR4Application: Flow CytometryProduct: Human CXCR4 Fluorescein-conjugated Monoclonal Antibody Catalog # FAB170FReference(s):Krathwohl, M.D. & J.L. Kaiser (2004) Chemokines promote quiescence and survival of human neural progenitor cells. Stem Cells 22:109.Sample Type: human neural progenitor cells

Product: Human CXCR4 Monoclonal Antibody Catalog # MAB173Reference(s):Hatse, S. et al. (2003) Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry. FEBS Lett. 546:300.Sample Type: human U87.CD4 astroglioma cell line stabily expressing CXCR4 variants

Application: ImmunohistochemistryProduct: Human CXCR4 Monoclonal Antibody Catalog # MAB170Reference(s):Lawrence, D.M. et al. (2004) Human immunodeficiency virus type 1 infection of human brain-derived progenitor cells. J. Virol. 78:7319.Sample Type: human brain-derived progenitor cells

Boutet, A. et al. (2001) Cellular expression of functional chemokine receptor CCR5 and CXCR4 in human embryonic neurons. Neurosci. Lett. 311:105.Sample Type: human mixed brain cells

FGF R1Application: Bioassay - blocking FGF signaling effectsProduct: Recombinant Human FGF R1 b (IIIc)/Fc Chimeric Protein Catalog # 661-FRReference(s):Kessaris, N. et al. (2004) Cooperation between sonic hedgehog and fibroblast growth factor/MAPK signaling pathways in neocortical precursors. Development 131:1289.Sample Type: mouse neocortical cells

Galectin-1Application: Immunohistochemistry & In VivoProduct: Mouse Galectin-1 Polyclonal Antibody Catalog # AF1245Reference(s):Plachta, N. et al. (2007) Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells. Nat. Neurosci. 10:712.Sample Type: rat brain and mouse sciatic nerve & rat

Application: Western BlotProduct: Mouse Galectin-1 Monoclonal Antibody Catalog # MAB1245Reference(s):Plachta, N. et al. (2007) Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells. Nat. Neurosci. 10:712.Sample Type: mouse neuron lysate

MAG/Siglec-4aApplication: Bioassay - neurite outgrowth assayProduct: Recombinant Rat MAG/Siglec-4a/Fc Chimeric Protein Catalog # 538-MGReference(s):Wright, K.T. et al. (2007) Bone marrow stromal cells stimulate neurite outgrowth over neural pro-teoglycans (CSPG), myelin associated glycoprotein and Nogo-A. Biochem. Biophys. Res. Commun. 354:559.Sample Type: chicken dorsal root ganglia

Benson, M.D. et al. (2005) Ephrin-B3 is a myelin-based inhibitor of neurite outgrowth. Proc. Natl. Acad. Sci. USA 102:10694.Sample Type: mouse neurons

Application: Immunohistochemistry

MAG Expression in Rat Cerebellum. MAG was detected in cryostat sections of rat cerebellum using anti-rat MAG polyclonal antibody (Catalog # AF538). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Application: ImmunoprecipitationProduct: Recombinant Rat MAG/Siglec-4a/Fc Chimeric Protein Catalog # 538-MGReference(s):Venkatesh, K. et al. (2005) The Nogo-66 receptor homolog NgR2 is a sialic acid-dependent receptor selective for myelin-associated glycoprotein. J. Neurosci. 25:808.Sample Type: African green monkey COS-7 kidney fibroblast cells transfected with human or mouse Nogo-66 Receptors -1, -2, or -3

Application: Western BlotProduct: Rat MAG/Siglec-4a Polyclonal Antibody Catalog # AF538Reference(s):Chen, S. et al. (2006) Neuregulin 1-erbB signaling is necessary for normal myelination and sensory function. J. Neurosci. 26:3079.Sample Type: mouse sciatic nerve homogenate


NEURAL STEM CELL & DIFFERENTIATION NestinApplication: Flow CytometryProduct: Human Nestin Monoclonal Antibody Catalog # MAB1259Reference(s):Ni, H.T. et al. (2006) High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells. Neuroreport 152:159.Sample Type: human neural precursor cells

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Detection of Intracellular Nestin using Flow Cytometry. Differentiated rat cortical cells were stained with phycoerythrin(PE)-conjugated mouse anti-rat Nestin monoclonal antibody (Catalog # IC2736P; filled histogram). Staining with PE-conjugated isotype control (Catalog # IC003P; open histogram) highlights the specificity of the nestin antibody.

Application: ImmunohistochemistryProduct: Human Nestin Monoclonal Antibody Catalog # MAB1259Reference(s):Oka, N. et al. (2007) VEGF promotes tumorigenesis and angiogenesis of human glioblastoma stem cells. Biochem. Biophys. Res. Commun. 360:553.Sample Type: human glioblastoma

Kim, B.K. et al. (2006) Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. FEBS Lett. 580:5869.Sample Type: human neural progenitor cells

Nestin Expression in Glioblastoma Cells. Nestin was detected in human glioblastoma cells using anti-human Nestin monoclonal antibody (Catalog # MAB1259). Cells were stained with a rhodamine-conjugated anti-mouse secondary antibody.







PRIMERPAIRS


MAP� HMR

Mash� M M M

MBP H

Meteorin M M M

MOG HM HM HM

Musashi-� H H

Musashi-� H H

NCAM-L� H H H H

Nectin-�/CD�� H H

Nestin HR R HR HMR

NeuroD� H H

NeuroD� H H

Neurofascin R

Neurogenin-� H

Neurogenin-� HR

Neurogenin-3 H

NF-H H H H

NF-L H

NF-M H H

NGFR/TNFRSF�6 H HM HM HM HMR

NGFR/TNFRSF�6 H HM HM HM HMR

NgR3/NgRH� H H H

NogoReceptor HM HM HM HM

Notch-�* R HR

Notch-�* HR HR R HR

Nucleostemin HMR

Olig� H H H

Olig� H H

Olig3 HM H H

*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/NotchPathwayforcompletelisting.


NucleosteminApplication: Immunohistochemistry

Identification of Nucleostemin-Positive Stem Cells. Nucleostemin was detected in the nuceoli of human U2OS cells using anti-human/mouse/rat Nucleostemin polyclonal antibody (Catalog # AF1638). Cells were stained with a rhodamine-conjugated donkey anti-goat secondary anti-body (red) and counterstained with DAPI (blue).

Oligodendrocyte Marker O1Application: ImmunohistochemistryProduct: Oligodendrocyte Marker O1 Monoclonal Antibody Catalog # MAB1327Reference(s):Rosin, C. et al. (2004) Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms. J. Neurochem. 90:1173.Sample Type: human oligodendrocytes

Kang, S.M. et al. (2007) Efficient induction of oligodendrocytes from human embryonic stem cells. Stem Cells 25:419.Sample Type: human neural progenitor cells

Oligodendrocyte Marker O4Application: Flow Cytometry

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O4

101 102 103 104

Detection of O4 Expression by Flow Cytometry. Differentiated rat cortical cells were stained with phycoerythrin(PE)-conjugated anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # FAB1326P; filled histogram). Staining with PE-conjugated isotype control (Catalog # IC003P; open histogram) highlights the specificity of the O4 antibody.

Oligodendrocyte Marker O4, continuedApplication: ImmunohistochemistryProduct: Oligodendrocyte Marker O4 Monoclonal Antibody Catalog # MAB1326Reference(s):Gossrau, G. et al. (2007) Bone morphogenetic protein-mediated modulation of lineage diversifica-tion during neural differentiation of embryonic stem cells. Stem Cells 25:939.Sample Type: mouse embryonic stem cell-derived oligodendrocytes

Zhang, Y. et al. (2007) Loss of testicular orphan receptor 4 impairs normal myelination in mouse forebrain. Mol. Endocrinol. 21:908. Sample Type: mouse brain

Benzing, C. et al. (2006) Neural conversion of human embryonic stem cell colonies in the presence of fibroblast growth factor-2. Neuroreport 17:1675.Sample Type: human embryonic stem cell-derived neurospheres

Tsai, H.H. et al. (2003) Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal. Develop-ment 130:2095.Sample Type: rat and chicken oligodendrocytes

PDGF RaApplication: ImmunohistochemistryProduct: Mouse PDGF Ra Polyclonal Antibody Catalog # AF1062Reference(s):Chojnacki, A. & S. Weiss (2004) Isolation of a novel platelet-derived growth factor-responsive precursor from the embryonic ventral forebrain. J. Neurosci. 24:10888.Sample Type: mouse brain

Gregg, C. et al. (2007) White matter plasticity and enhanced remyelination in the maternal CNS. J. Neurosci. 27:1812.Sample Type: mouse brain, optic nerve, and spinal cord

Product: Human PDGF Ra Polyclonal Antibody Catalog # AF-307-NAReference(s):Uemura, A. et al. (2006) Tlx acts as a proangiogenic switch by regulating extracellular assembly of fibronectin matrices in retinal astrocytes. J. Clin. Invest. 116:369.Sample Type: mouse retinal caps

Product: Human PDGF Ra Monoclonal Antibody Catalog # MAB322Reference(s):Luyt, K. et al. (2004) Metabotropic glutamate receptors are expressed in adult human glial progeni-tor cells. Biochem. Biophys. Res. Commun. 319:120.Sample Type: human glial progenitor and primary hippocampal neuron cells

Application: NeutralizationProduct: Human PDGF Ra Polyclonal Antibody Catalog # AF-307-NAReference(s):Chittajallu, R. et al. (2005) Downregulation of platelet-derived growth factor-a receptor-mediated tyrosine kinase activity as a cellular mechanism for K+-channel regulation during oligodendrocyte development in situ. J. Neurosci. 25:8601.Sample Type: mouse coronal slice

USA & Canada (800) 343-7475 | UK & Europe +44 (0)1235 529449 | www.RnDSystems.com �9



NEURAL STEM CELL & DIFFERENTIATION SOX2Application: Flow Cytometry

0

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120

100

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SOX2

101 102 103 104

Detection of Intracellular SOX2 in NTera2 Cells using Flow Cytometry. Human embryonal carcinoma NTera2 cells were stained with allophycocyanin(APC)-conjugated mouse anti-hu-man SOX2 monoclonal antibody (Catalog # IC2018A; filled histogram). Staining with APC-con-jugated isotype control (Catalog # IC003A; open histogram) highlights the specificity of the SOX2 antibody.

Application: ImmunohistochemistryProduct: Human/Mouse SOX2 Monoclonal Antibody Catalog # MAB2018Reference(s):Kobayashi, T. et al. (2006) Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Stroke 37:2361.Sample Type: rat neurospheres

SOX2 Expression in NTera-2 Cells. SOX2 was detected in human embryonal teratocarcinoma NTera-2 cells using anti-human SOX2 monoclonal antibody (Catalog # MAB2018). Cells were stained using Alexa Fluor® 568-conjugated anti-mouse secondary antibody (red). The nuclei were counterstained with DAPI (blue). Image courtesy of Jingli Cai and Mahendra Rao, National Institutes of Health.

SOX10Application: ImmunohistochemistryProduct: Human SOX10 Polyclonal Antibody Catalog # AF2864Reference(s):Nait-Oumesmar, B. et al. (2007) Activation of the subventricular zone in multiple sclerosis: evidence for early glial progenitors. Proc. Natl. Acad. Sci. USA 104:4694.Sample Type: human brain


Primary Rat Cortical Stem Cells. Ex vivo expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders. R&D Systems now offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. Every lot of R&DSystemsRatCorticalStemCells (Catalog # NSC001) exhibits a high level of nestin expression and has the capacity for multi-lineage differen-tiation (astrocytes, neurons, and oligodendrocytes). Our rigorous testing procedures ensure the highest level of quality and consistency from lot to lot. Depending on your research needs, our Rat Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.





PRIMERPAIRS

OligodendrocyteMarkerO�

HMRCh

OligodendrocyteMarkerO4

HMRCh HMRCh

OMgp HM HM HM HM

Pax3 HM H HM

Pax6 MRCh MRCh

PDGFRa* HM HM HM HM H

PLP HMR

Prominin� H

ROBO3 H H H HM

S�00B H

Snail H

SOX� H H

SOX� HM H H HMR

SOX�0 HR H H

SOX�� H H

SSEA-� HM HM

SSEA-4 HM HM

TRAF-4 H H

TROY/TNFRSF�9 M HM HM HM

b-IIITubulin Ms Ms

TyrosineHydroxylase

Ms MR

Vimentin H H HMR

*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/PDGFFamilyforcompletelisting.

Alexa Fluor is a registered trademark of Molecular Probes, Inc.


USA & Canada (800) 343-7475 | UK & Europe +44 (0)1235 529449 | China (21) 52380373 | www.RnDSystems.com ��

SSEA-4Application: ImmunohistochemistryProduct: Human/Mouse Stage-specific Embryonic Ag-4 Monoclonal Antibody Catalog # MAB1435Reference(s):Kim, B.K. et al. (2006) Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. FEBS Lett. 580:5869.Sample Type: human embryonic stem cells

SSEA-4 Expression in Embryonic Stem Cells. Human BG01V embryonic stem cells were cul-tured with recombinant human FGF basic (Catalog # 233-FB) in the presence (left) or absence (right) of Mouse Embryonic Fibroblast (MEF)-conditioned medium (Catalog # AR005). SSEA-4 and Oct-3/4 were detected using anti-human SSEA-4 monoclonal antibody (Catalog # MAB1435) and anti-human Oct-3/4 polyclonal antibody (Catalog # AF1759). Cells were stained with Alexa Fluor 568-conjugated anti-mouse secondary antibody (SSEA-4; red) and Alexa Fluor 488-conjugated anti-goat secondary antibody (Oct-3/4; green). Cells were counterstained with DAPI (blue). Image courtesy of Dr. Frank Soldner of the National Institutes of Health.

TROY/TNFRSF19Application: Flow CytometryProduct: Human TROY/TNFRSF19 Polyclonal Antibody Catalog # AF1548Reference(s):Fry, E.J. et al. (2007) A role for Nogo receptor in macrophage clearance from injured peripheral nerve. Neuron 53:649.Sample Type: human monocytes

b-III TubulinApplication: ImmunohistochemistryProduct: Neuron-specific b-III Tubulin Monoclonal Antibody Catalog # MAB1195Reference(s):Nakamura, K. et al. (2006) Expression of olig2 in retinal progenitor cells. Neuroreport 17:345.Sample Type: mouse retina

Zhang, J.Q. et al. (2006) Neural differentiation of embryonic stem cells induced by conditioned medium from neural stem cells. Neuroreport 17:981.Sample Type: mouse embryonic stem cell-derived neurons

Hagimura, N. et al. (2004) Predominant expression of GluR2 among the AMPA receptor subunits in neuronal progenitor cells of the rat hippocampus. Brain Res. Dev. Brain Res. 152:213.Sample Type: rat neurons

The Human (Catalog # SC011) and Mouse/Rat (Catalog # SC013) Neural Stem Cell Functional Identification Kits contain specially formulated media supplements that can be used for the short-term maintenance and expansion of NSCs, and the differentiation of NSCs into astrocyte, neuron, and oligodendrocyte lineages. Also included is an antibody panel consisting of anti-Nestin, anti-Glial Fibrillary Acidic Protein (GFAP), anti-Neuron-specific b-III Tubulin, and anti-Oligodendrocyte Marker O4 to identify neural precursors, astrocytes, neurons, and oligo-dendrocytes, respectively.

NEURALSTEMCELLFUNCTIONALIDENTIFICATIONKITS

Identification of Neural Cell Populations. Rat cortical stem cells (Catalog # NSC001) exhibit high expression of Nestin as detected with goat anti-rat nestin polyclonal antibody (Figure 1). Cells were stained with a Rhodamine Red-conjugated secondary antibody and counterstained with Fluoro Nissl Green. Distinct cell populations were identified in differ-entiated neural stem cells: Neurons were labeled detected using mouse anti-neuron- specific b-III Tubulin monoclonal antibody (Figure 2A; red). Astrocytes were labeled using mouse anti-human GFAP polyclonal antibody (Figure 2A; green). Oligodendrocytes were identified using mouse anti-Oligodendrocyte Marker O4 monoclonal antibody (Fig-ure 2B). All primary antibodies are included in the Mouse/Rat Neural Stem Cell Functional Identification Kit (Catalog # SC011).

Neural Stem CellsPlated on Polyornithine/Fibronectin-coated plates for

48 hrs in medium containing maintenance supplement.

2A.

1.

Cultured for 7-10 days in medium containing differentiation supplement.

2B.

�� For research use only. Not for use in diagnostic procedures.


NEURAL TRANSMISSIONMOLECULE MONOCLONAL

ANTIBODIESPOLYCLONALANTIBODIES



ELISA/ASSAY

Neurotransmitters,NeuroactiveMolecules,&AssociatedEnzymes

Adiponectin/Acrp30

H HR HMR HM Hm

CART H H

CholineAcetyltransferase/ChAT

H H H

Cortisol Ms

DopaDecarboxylase/DDC

H H H H

Endothelin-� H H

GAD�/GAD67 H H H

GAD�/GAD65 H

Glucagon HM

GrowthHormone HR HR HR H H

Kininogen HM H H HM

Leptin H HM HM HMR HM

LeukotrieneB4 Ms

MANF H

NitricOxide Ms

OrexinA HMR HMR

OrexinB H

ProstaglandinE� Ms

PSMA/FOLH� H

SubstanceP H Ms

TRH-degradingEctoenzyme/TRHDE

M M

TryptophanHydroxylase

HMRCaPrXMs

TyrosineHydroxylase

Ms MR

Adiponectin/Acrp30Application: Bioassay - effect on release of pituitary hormonesProduct: Recombinant Mouse gAdiponectin/gAcrp30 Protein Catalog # 1119-ACReference(s):Rodriguez-Pacheco, F. et al. (2007) Regulation of pituitary cell function by adiponectin. Endocrinol-ogy 148:401.Sample Type: rat anterior pituitary cells

Agrin Application: Bioassay- acetylcholine receptor (AChR) clusteringProduct: Recombinant Rat Agrin Protein (see figure page 25) Catalog # 550-AGReference(s):Ponomareva, O.N. et al. (2006) Defective neuromuscular synaptogenesis in mice expressing consti-tutively active ErbB2 in skeletal muscle fibers. Mol. Cell. Neurosci. 31:334.Sample Type: mouse C2C12 myoblast cell line

Application: ImmunohistochemistryProduct: Rat Agrin Polyclonal Antibody Catalog # AF550Reference(s):Graf, E.R. et al. (2004) Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins. Cell 119:1013.Sample Type: rat neurons

AktApplication: Western BlotProduct: Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody Catalog # MAB2055Reference(s):Xu, W. et al. (2007) Calpain-mediated mGluR1a truncation: a key step in excitotoxicity. Neuron 53:399.Sample Type: rat neuron lysate

BDNF Application: In VivoProduct: Recombinant Human BDNF Protein Catalog # 248-BDReference(s):Paredes, D. et al. (2007) Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat. Brain Res. 1141:56.Sample Type: rat

CCK-A RApplication: ImmunohistochemistryProduct: Human/Mouse/Rat CCK-A R Polyclonal Antibody Catalog # AF2680Reference(s):Zheng, Z. et al. (2005) In vitro analysis of the effects of cholecystokinin on rat brain stem moto-neurons. Am. J. Physiol. Gastrointest. Liver Physiol. 288:G1066.Sample Type: rat brainstem




CFTRApplication: ImmunohistochemistryProduct: Human CFTR C-Terminus Monoclonal Antibody Catalog # MAB25031Reference(s):Weng, T.X. et al. (2002) Oxidant and antioxidant modulation of chloride channels expressed in human retinal pigment epithelium. Am. J. Physiol. Cell Physiol. 283:C839.Sample Type: human eye

Choline Acetyltransferase (ChAT)Application: Immunohistochemistry

Product: Human ChAT Polyclonal Antibody Catalog # AF3447Reference(s):Zhang, J.Q. et al. (2006) Neural differentiation of embryonic stem cells induced by conditioned medium from neural stem cell. Neuroreport 17:981.Sample Type: mouse neuronal stem cells

ChAT Expression in Human Brain. ChAT was detected in paraffin-embedded human brain (cortex) tissue sections using anti-human ChAT polyclonal antibody (Catalog # AF3447). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

CREBApplication: Immunohistochemistry

CREB Expression in Human Dorsal Root Ganglion (DRG). CREB was detected in paraffin-embedded sections of human DRG using anti-human CREB polyclonal antibody (Catalog # AF2989). Samples were subjected to antigen retrieval using R&D Systems basic antigen retrieval reagent (Catalog # CTS013). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

ERK1/2Application: Immunohistochemistry

Phosphorylated ERK1/ERK2 Expression in Rat Brain Cortex. Inflammation-induced phos-phorylated ERK1/ERK2 was detected in cryostat tissue sections of rat brain cortex using anti-human/mouse/rat phospho-ERK1/ERK2 polyclonal antibody (Catalog # AF1018). Tissue was stained using the anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).

Frizzled-4Application: ImmunohistochemistryProduct: Human/Mouse Frizzled-4 Monoclonal Antibody Catalog # MAB194Reference(s):Chen, J. et al. (2006) Activity-dependent synaptic Wnt release regulates hippocampal long term potentiation. J. Biol. Chem. 281:11910.Sample Type: mouse hippocampus

KORApplication: Immunohistochemistry

Kappa-Opioid Receptor (KOR) Expression in Human Brainstem. KOR was detected in paraffin-embedded normal human brainstem (medulla) tissue sections using anti-human KOR monoclonal antibody (Catalog # MAB38951). Samples were subjected to antigen retrieval using the Basic Anti-gen Retrieval kit (Catalog # CTS013). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).



NEURAL TRANSMISSION LeptinApplication: Bioassay - effect on peptide releaseProduct: Recombinant Rat Leptin Protein Catalog # 598-LPReference(s):Breen, T.L. et al. (2005) Effects of fasting, leptin, and insulin on AGRP and POMC peptide release in the hypothalamus. Brain Res. 1032:141.Sample Type: rat hypothalamus

Application: Bioassay - effect on synaptic activityProduct: Recombinant Human Leptin Protein Catalog # 398-LPReference(s):Durakoglugil, M. et al. (2005) Leptin induces a novel form of NMDA receptor-dependent long-term depression. J. Neurochem. 95:396.Sample Type: rat hippocampal slice


Leptin Expression in Human Brain. Leptin was detected in paraffin-embedded human brain sections using anti-human Leptin monoclonal antibody (Catalog # MAB398). Tissue was stained with the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog #CTS002; brown) and coun-terstained with hematoxylin (blue).

NF-HApplication: Immunohistochemistry

NF-H Expression in Human Brain. NF-H was detected in paraffin-embedded human brain (cerebellum) tissue sections using the anti-human NF-H polyclonal antibody (Catalog # AF3108). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).



BIOTINYLATEDANTIBODIES

FLUOROCHROMELABELEDANTIBODIES


NeurotransmitterReceptors,IonChannels&OtherTransmembraneProteins

CannabinoidR� H

CannabinoidR�/CB�/CNR�

H

CCK-AR R H HMR

CFTR H

Connexin43 HMRPr

DPP�0 H

DPP6 H H H

GABA-A-Ra�,�,4,5,6

HMRB

GABA-A-Rb�,�,3 HMR

GABA-A-Rg� HMRB

GABA-B-R� R R

GLP-�R H

GluR� HMR

GluR� HMR

GluR�/3 HMR

GluR3 HMR

Glut� H

Glut� HM M

Glut3 H H

Glut4 R R

Glut5 H H

KOR H

LeukotrieneB4R� H H

NMDAR,NR�Subunit

HMR HMR

NMDAR,NR�ASubunit

HMR

NMDAR,NR�BSubunit

HMRPr

NMDAR,NR�CSubunit

HMR

PAR� H

PAR� H H

PotassiumChannelKv�.�

MRX

PotassiumChannelKv3.�

R

RAGE HMR HMR HM HMR

SodiumCalciumExchanger�

Ms

TrkA* HR HMR HR H

VanilloidR� R

VanilloidR-like3 H


*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/Neurotrophinsforcompletelisting.



MIS/AMHApplication: ImmunohistochemistryProduct: Rat MIS/AMH Polyclonal Antibody Catalog # AF1446Reference(s):Wang, P.Y. et al. (2005) Müllerian inhibiting substance acts as a motor neuron survival factor in vitro. Proc. Natl. Acad. Sci. USA 102:16421.Sample Type: mouse lumbar spinal cord, ovaries, and testes

MIS RIIApplication: Immunoprecipitation & Western blotProduct: Rat MIS RII Polyclonal Antibody Catalog # AF1618Reference(s):Wang, P.Y. et al. (2005) Müllerian inhibiting substance acts as a motor neuron survival factor in vitro. Proc. Natl. Acad. Sci. USA 102:16421.Sample Type: mouse lumbar spinal cord, ovary, and testical homogenates

MuSKApplication: NeutralizationProduct: Rat MuSK Polyclonal Antibody Catalog # AF562Reference(s):Finn, A.J. et al. (2003) Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction. Nat. Neurosci. 6:717.Sample Type: mouse C2C12 myoblast cell line lysate, homogenized brain, and muscle

A B

MuSK mediates Agrin-Induced Clustering. A: Myotubes differentiated from the C2C12 murine myoblast cell line were treated with recombinant rat Agrin (Catalog # 550-AG) to cluster acetylcholine receptors (AchRs). B: Pre-treatment with goat anti-rat MuSK polyclonal antibody (Catalog # AF562) inhibits agrin-induced clustering. Cells were stained using a rhodamine a-bungarotoxin conjugate.

Application: Western BlotProduct: Rat Polyclonal Antibody Catalog # AF562Reference(s):Garcia-Osta, A. et al. (2006) MuSK expressed in the brain mediates cholinergic responses, synaptic plasticity, and memory formation. J. Neurosci. 26:7919.Sample Type: rat brain, muscle, liver, lung, heart, and spleen tissue homogenates; mouse C2C12 myoblast cell line lysate

NMDA R, NR2B SubunitApplication: Western Blot

— 200

— 100

— 68

CONTROL

λ-PPASE

NR2B —

Phosphorylation of NMDA Receptor (NR2B Subunit) in Rat Hippocampus. Rat hippocampal lysate was assessed by Western blot using rabbit anti-human/mouse/rat phospho-NMDA receptor NR2B Subunit (Y1336) polyclonal antibody (Catalog # PPS057). Immunolabeling is absent in samples pre-treated with l-phosphatase (PPase), indicating the phosphospecificity of this antibody.

NT-4Application: Bioassay - TrkB activationProduct: Recombinant Human NT-4 Protein Catalog # 268-N4Reference(s):Choi, S.Y. et al. (2004) NR2A induction and NMDA receptor-dependent neuronal death by neuro-trophin-4/5 in cortical cell culture. J. Neurochem. 88:708.Sample Type: mouse neurons and astrocytes

Orexin AApplication: ImmunohistochemistryProduct: Human/Mouse/Rat Orexin A Monoclonal Antibody Catalog # MAB763Reference(s):Georgescu, D. et al. (2003) Involvement of the lateral hypothalamic peptide orexin in morphine dependence and withdrawal. J. Neurosci. 23:3106.Sample Type: mouse brain

RAGE Application: Bioassay - Biacore binding assayProduct: Recombinant Human RAGE/Fc Chimeric Protein Catalog # 1145-RGReference(s):Kiryushko, D. et al. (2006) Molecular mechanisms of Ca2+ signaling in neurons induced by the S100A4 protein. Mol. Cell. Biol. 26: 3625.Sample Type: recombinant human S100A4 and S100A12 proteins



NEURAL TRANSMISSION Substance PApplication: ELISA KitProduct: Substance P Parameter Assay Kit Catalog # KGE007Reference(s):Bruno, M.A. & A.C. Cuello (2006) Activity-dependent release of precursor nerve growth factor, conversion to mature nerve growth factor, and its degradation by a protease cascade. Proc. Natl. Acad. Sci. USA 103:6735.Sample Type: rat cerebral tissue perfusate


Substance P in Human Spinal Cord. Substance P was detected in paraffin-embedded normal human spinal cord tissue sections using anti-human/mouse/rat Substance P monoclonal anti-body (Catalog # MAB4375). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Synuclein-aApplication: ImmunohistochemistryProduct: Human Synuclein-a Polyclonal Antibody Catalog # AF1338Reference(s):Langerveld, A.J. et al. (2007) Gene expression changes in postmortem tissue from the rostral pons of multiple system atrophy patients. Mov. Disord. 22:766.Sample Type: human brain

TNF-a/TNFSF1AApplication: Bioassay - NMDA and TNF receptor activationProduct: Recombinant Mouse TNF-a/TNFSF1A Protein Catalog # 410-MTReference(s):Jara, J.H. et al. (2007) Tumor necrosis factor a stimulates NMDA receptor activity in mouse cortical neurons resulting in ERK-dependent death. J. Neurochem. 100:1407.Sample Type: mouse E16 neurons

Application: ELISA KitProduct: Mouse TNF-a/TNFSF1A DuoSet Catalog # DY410Reference(s):Ponath, G. et al. (2007) Autocrine S100B effects on astrocytes are mediated via RAGE. J. Neuroim-munol. 184:214.Sample Type: mouse astrocyte supernatant



BIOTINYLATEDANTIBODIES

FLUOROCHROMELABELEDANTIBODIES


Synapse-RelatedMolecules

Agrin R R R R

Dynamin HMR

GRASP HMR

IGSF4A/SynCAM M M M HM

IGSF4B H

IGSF4C H H

LamininS HRCh

MuSK R HR R

N-Cadherin H H

NCAM-�/CD56 H H H H

NCAM-L� H H H H

Nectin-� H H

Nectin-�/CD�� H H

Nectin-3 H HM

Nectin-4 HM HM H H

N-WASP H

PSD-95 HMR

S�00B HM

SynapsinI HMRBX

Synaptotagmin R

Syndecan-� H H H

Synuclein-a H H H

Thrombospondin-� H H

Thrombospondin-� H H H

SignalTransduction

Akt HMR HMR HMR HMR

AMPKa� HMR HMR

AMPKa� HMR

AMPKb� HMR

CaMKinaseII HMRBChX

cAMP Ms

CREB H HM HMR

ERK�/ERK�* HMR HMR HMR

JAK�* HMR MR

JNK* HMR HMR HMR

p38a* HMR HMR HMR

PI3-kinasep85a* HMR

STAT3* H HMR HM


Pleasevisitwww.RnDSystems.com/go/signaltransductionforcompletelistingofoursignaltransductionproducts.

*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/IntracellularKinasesforcompletelisting.



TNF RI/TNFRSF1AApplication: Bioassay - agonist activityProduct: Mouse TNF RI/TNFRSF1A Polyclonal Antibody Catalog # AF-425-PBReference(s):Jara, J.H. et al. (2007) Tumor necrosis factor a stimulates NMDA receptor activity in mouse cortical neurons resulting in ERK-dependent death. J. Neurochem. 100:1407.Sample Type: mouse E16 neurons

TNF RII/TNFRSF1BApplication: Flow Cytometry & ImmunohistochemistryProduct: Mouse TNF RII/TNFRSF1B Polyclonal Antibody Catalog # AF-426-PBReference(s):Pollock, J. et al. (2002) TNF-a receptors simultaneously activate Ca2+ mobilisation and stress kinases in cultured sensory neurons. Neuropharmacology 42:93.Sample Type: rat dorsal root ganglia

TrkAApplication: Immunohistochemistry

TrkA Expression in Rat Dorsal Root Ganglion (DRG). TrkA was detected in cryostat sections of rat DRG using anti-rat TrkA polyclonal antibody (Catalog # AF1056). Tissue was stained using a Rhodamine Red-conjugated anti-goat secondary antibody. TrkA labeling is confined to the neuronal plasma membrane.

TrkBApplication: In Vivo & Bioassay - blocking of BDNFProduct: Recombinant Human TrkB/Fc Chimeric Protein Catalog # 688-TKReference(s):Rex, C.S. et al. (2007) Brain-derived neurotrophic factor promotes long-term potentiation-related cytoskeletal changes in adult hippocampus. J. Neurosci. 27:3017.Sample Type: rat & rat hippocampal slice

Kinases are integral parts of signal transduction cascades that regulate virtually all cellular activities. Therefore, it is of critical importance to have quality tools that make it easy to study kinase activity. R&D Systems Proteome Profiler Antibody Arrays are screening tools designed to detect the phosphorylation of multiple different proteins in a single sample. Our Phospho-MAPK Antibody Array (Catalog # ARY002) allows for the simultaneous detection of the relative phosphorylation of 18 different serine/threonine kinases and avoids time-consuming IP/Western blots. Each human Phospho-MAPK Antibody Array Kit contains buffers, detection antibodies, streptavidin-HRP, and 4 membranes spotted in duplicate with carefully selected, highly specific capture antibodies.

PROTEOME-PROFILER™HUMANPHOSPHO-MAPKARRAY

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Sorbitol-treated

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Sorbitol -treatedER

K1

ERK2

JN

K1

JNK2

JN

K3

JNKp

an

MSK

2 RS

K1

RSK2

p3

8γ

p38δ

p3

8α

p38β

GS

K-3α

/β

GSK-

3β

Akt1

Akt2

Akt3

Akt p

an

HSP2

7 p7

0S6K

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Sorbitol -treatedER

K1

ERK2

JN

K1

JNK2

JN

K3

JNKp

an

MSK

2 RS

K1

RSK2

p3

8γ

p38δ

p3

8α

p38β

GS

K-3α

/β

GSK-

3β

Akt1

Akt2

Akt3

Akt p

an

HSP2

7 p7

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SIMULTANEOUSLYDETECTTHEACTIVITYOFTHESEKINASES

ERK� p38a Akt� ERK� p38b Akt� JNK�

p38g Akt3 JNK� p38d Aktpan JNK3 RSK�

GSK-3a/b JNKpan RSK� GSK-3b MSK� p70S6K HSP�7

MAPK Phosphorylation in PC12 Cells. The human Phospho-MAPK Array (Catalog # ARY002) detects multiple phosphorylated kinases in lysates from untreated and sorb-itol-treated cells. Array signals from scanned X-ray film images were analyzed using image analysis software.

Also available from R&D Systems is our Phospho-RTK Antibody Array (Catalog # ARY001) designed to simultaneously detect the relative phosphorylation of 42 different receptor tyrosine kinases (RTKs) in a single sample.

Formoreinformation,pleasevisitwww.RnDSystems.com/go/ProteomeProfiler



NEURODEGENERATIVE DISEASE & NEUROINFLAMMATION



BIOTINYLATED&FLUOROCHROME-LABELEDANTIBODIES


ELISA/ASSAYS

NEURODEGENERATIVEDISEASE

�4-3-3* HMRX

ADAM9 HM HM HM HM

ADAM�0 HM HM HM HM

TACE/ADAM�7 H H HM H

ADAMTS4 H H

APLP-� HM HM M

APLP-� M

APP HM HMRCaPr H HMR

ArylsulfataseA/ARSA

H H H HM

BACE-� HM H H HM

BACE-� HM M

Bag-� H H

Bax HMR HM H

Caspase-�0 H H H Ms

CathepsinB HM HM HM HM H

CathepsinD HM HM HM HM

CathepsinE HM HM HM HM

Claudin-�� H

Claudin-�� H

CystatinC HM HM HM HM H

DARPP-3� MR MR

DopaDecarboxylase/DDC

H H H H

ECE-� H H H H

ECE-� H H H

F-Spondin H H

FPRL� H H

Insulysin/IDE H H H

Laminina� H

a�-Macroglobulin H H H H


MBP H

MOG HM HM HM

Myeloperoxidase HM H H

N-Me-6,7-diOH-TIQ Ms

Neprilysin/CD�0 HM HM HM HM HM

Neurolysin H

NF-H* H H H

Continued on page 30

ADAM15Application: Immunohistochemistry

ADAM15 Expression in Mouse Cerebellum. ADAM15 was detected in cryostat sections of mouse cerebellar Purkinje cells using anti-mouse ADAM15 polyclonal antibody (Catalog # AF945). Tissue was stained with Cy™3-conjugated anti-goat antibody (red) and counter-stained with FluoroNissl green.

Bag-1Application: ImmunohistochemistryProduct: Human Bag-1 Monoclonal Antibody Catalog # MAB852Reference(s):Jana, N.R. & N. Nukina (2005) BAG-1 associates with the polyglutamine-expanded huntingtin aggregates. Neurosci. Lett. 378:171.Sample Type: human brain

BaxApplication: Western BlotProduct: Human/Mouse Bax Polyclonal Antibody Catalog # AF820Reference(s):Jayanthi, S. et al. (2001) Methamphetamine causes differential regulation of pro-death and anti-death bcl-2 genes in the mouse neocortex. FASEB J. 15:1745.Sample Type: mouse frontal cortex homogenate


*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/Neurodegenerativeforcompletelisting.

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Caspase-3Application: Bioassay - caspase activity assayProduct: Caspase-3 Colorimetric Assay Kit Catalog # BF3100Reference(s):Djebaili, M. et al. (2004) Allopregnanolone and progesterone decrease cell death and cognitive deficits after a contusion of the rat pre-frontal cortex. Neuroscience 123:349.Sample Type: rat brain homogenate

Application: ImmunohistochemistryProduct: Human/Mouse Active Caspase-3 Polyclonal Antibody Catalog # AF835Reference(s):Palzur, E. et al. (2004) Hyperbaric oxygen therapy for reduction of secondary brain damage in head injury: an animal model of brain contusion. J. Neurotrauma 21:41.Sample Type: rat brain

Caspase-10Application: Western BlotProduct: Human Caspase-10/a (Mch4) Monoclonal Antibody Catalog # MAB834Reference(s):Qiu, J. et al. (2002) Upregulation of the Fas receptor death-inducing signaling complex after traumatic brain injury in mice and humans. J. Neurosci. 22:3504.Sample Type: mouse brain homogenate

CCL2/MCP-1Application: Bioassay - membrane potential assayProduct: Recombinant Mouse CCL2/JE/MCP-1 Protein Catalog # 479-JEReference(s):White, F.A. et al. (2005) Excitatory monocyte chemoattractant protein-1 signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion. Proc. Natl. Acad. Sci. USA 102:14092.Sample Type: mouse dorsal root ganglia

Application: ELISA KitProduct: Human CCL2/MCP-1 Quantikine ELISA Kit Catalog # DCP00Reference(s):Vargas, D.L. et al. (2005) Neuroglial activation and neuroinflammation in the brain of patients with autism. Ann. Neurol. 57:67.Sample Type: human brain homogenate

Product: Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit Catalog # MJE00Reference(s):Qin, L. et al. (2007) Systemic LPS causes chronic neuroinflammation and progressive neurodegen-eration. Glia 55:453.Sample Type: mouse brain homogenate

CCL2/MCP-1, continuedApplication: ImmunohistochemistryProduct: Mouse CCL2/JE/MCP-1 Polyclonal Antibody Catalog # AF-479-NAReference(s):Mbebi, C. et al. (2005) Antibody-bound b-amyloid precursor protein stimulates the production of tumor necrosis factor-a and monocyte chemoattractant protein-1 by cortical neurons. Neurobiol. Dis. 19:129.Sample Type: mouse astrocytes and oligodendrocytes

Application: In Vivo Product: Mouse CCL2/JE/MCP-1 Monoclonal Antibody Catalog # MAB479Reference(s):Sheehan, J.J. et al. (2007) Proteolytic activation of monocyte chemoattractant protein-1 by plasmin underlies excitotoxic neurodegeneration in mice. J. Neurosci. 27:1738.Sample Type: mouse

CCL3/MIP-1aApplication: ImmunohistochemistryProduct: Mouse CCL3/MIP-1a Polyclonal Antibody Catalog # AF-450-NAReference(s):Kalkonde, Y.V. et al. (2007) Chemokines in the MPTP model of Parkinson’s disease: absence of CCL2 and its receptor CCR2 does not protect against striatal neurodegeneration. Brain Res. 1128:1.Sample Type: mouse brain

CCL4/MIP-1bApplication: ImmunohistochemistryProduct: Human CCL4/MIP-1b Biotinylated Polyclonal Antibody Catalog # BAF271Reference(s):Deiva, K. et al. (2004) Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apop-tosis in human neurons through extracellular signal-regulated kinase activation. Eur. J. Neurosci. 20:3222.Sample Type: human SK-N-SH neuroblastoma cell line and brain tissue

CCL5/RANTESApplication: ELISA DevelopmentProduct: Mouse CCL5/RANTES Biotinylated Polyclonal Antibody Catalog # BAF478Reference(s):Kremlev, S.G. & C. Palmer (2005) Interleukin-10 inhibits endotoxin-induced pro-inflammatory cytokines in microglial cell cultures. J. Neuroimmunol. 162:71.Sample Type: rat microglia supernatant





BIOTINYLATED&FLUOROCHROME-LABELEDANTIBODIES


ELISA/ASSAYS

NEURODEGENERATIVEDISEASE,continued

NMDAR,NR�Subunit

HMR HMR

NMDAR,NR�ASubunit

HMR

NMDAR,NR�BSubunit

HMRPr

NMDAR,NR�CSubunit

HMR

Nogo-A R HR HR

OMgp HM HM HM HM

p300 H

Park7/DJ-� HM

Parkin H H

PGE� Ms

Presenilin-� H H H H

Presenilin-� H H H

PSMA/FOLH� H

RAGE HMR HMR HM HMR HM

S�00A�3 H HM

S�00B H

a-Secretase Ms

b-Secretase Ms

g-Secretase Ms

SerpinA3 H H H

SerpinE� M HM HM HM

SMC� HMR H

SuperoxideDismutase

Ms

SuperoxideDismutase-�/Cu-ZnSOD

H HMR

SuperoxideDismutase-�/Mn-SOD

HMR

SynapsinI HMRBX

Synuclein-a H H H

Tau Ms Ms

TGF-b* Ms

THOP� H H H

TyrosineHydroxylase

Ms MR

Ubiquitin H H H H H

VEGF HMRCaZ HMRCaZ HHMRCaZ HMRCaZ HMRCa

CCL21/6CkineApplication: Western BlotProduct: Mouse CCL21/6Ckine Polyclonal Antibody Catalog # AF457Reference(s):Bagaeva, L.V. et al. (2006) CXC chemokine ligand 13 plays a role in experimental autoimmune encephalomyelitis. J. Immunol. 176:7676.Sample Type: mouse spinal cord homogenate

CCR2Application: ImmunohistochemistryProduct: Human CCR2 Monoclonal Antibody Catalog # MAB150Reference(s):Eugenin, E.A. et al. (2005) HIV-1 tat protein induces a migratory phenotype in human fetal micro-glia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS. Glia 49:501.Sample Type: human microglia

CCR5Application: Flow CytometryProduct: Human CCR5 Fluorescein-conjugated Monoclonal Antibody Catalog # FAB180FReference(s):Krathwohl, M.D. & J.L. Kaiser (2004) Chemokines promote quiescence and survival of human neural progenitor cells. Stem Cells 22:109.Sample Type: human neural progenitor cells

Application: ImmunohistochemistryProduct: Human CCR5 Monoclonal Antibody Catalog # MAB181Reference(s):Boutet, A. et al. (2001) Cellular expression of functional chemokine receptor CCR5 and CXCR4 in human embryonic neurons. Neurosci. Lett. 311:105.Sample Type: human embryonic mixed brain cells

CD14Application: ELISA DevelopmentProduct: Mouse CD14 Polyclonal Antibody Catalog # AF982Reference(s):Bate, C. et al. (2006) Prostaglandin D2 mediates neuronal damage by amyloid-b or prions which activates microglial cells. Neuropharmacology 50:229.Sample Type: mouse neuron lysate

CXCL8/IL-8Application: ELISA KitProduct: Human CXCL8/IL-8 Quantikine ELISA Kit Catalog # D8000CReference(s):Park, C. et al. (2006) TLR3-mediated signal induces proinflammatory cytokine and chemokine gene expression in astrocytes: differential signaling mechanisms of TLR3-induced IP-10 and IL-8 gene expression. Glia 53:248.Sample Type: human CRT-MG astroglioma cell line supernatant


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CXCR1/IL-8 RAApplication: Flow CytometryProduct: Human CXCR1/IL-8 RA Monoclonal Antibody Catalog # MAB330Reference(s):Goldberg-Bittman, L. et al. (2005) Cellular characteristics of neuroblastoma cells: regulation by the ELR-CXC chemokine CXCL10 and expression of a CXCR3-like receptor. Cytokine 29:105.Sample Type: human neuroblastoma cells

Application: ImmunohistochemistryProduct: Human CXCR1/IL-8 RA Monoclonal Antibody Catalog # MAB330Reference(s):Malgorzata Goczalik, I. et al. (2005) The activation of IL-8 receptors in cultured guinea pig Müller glial cells is modified by signals from retinal pigment epithelium. J. Neuroimmunol. 161:49.Sample Type: guinea pig Müller glial cells

CXCL10/IP-10/CRG-2Application: ELISA KitProduct: Human CXCL10/IP-10 DuoSet Catalog # DY266Reference(s):Rivieccio, M.A. et al. (2005) The cytokine IL-1b activates IFN response factor 3 in human fetal astrocytes in culture. J. Immunol. 174:3719.Sample Type: human brain astrocyte supernatant

Product: Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit Catalog # MCX100Reference(s):Nishimura, F. et al. (2006) Adoptive transfer of type 1 CTL mediates effective anti-central nervous system tumor response: critical roles of IFN-inducible protein-10. Cancer Res. 66:4478.Sample Type: mouse central nervous system tumor cell supernatant

Application: ImmunohistochemistryProduct: Mouse CXCL10/IP-10/CRG-2 Biotinylated Polyclonal Antibody Catalog # BAF466Reference(s):Bhowmick, S. et al. (2007) Induction of IP-10 (CXCL10) in astrocytes following Japanese encephali-tis. Neurosci. Lett. 414:45.Sample Type: mouse brain

CXCL12/SDF-1Application: Bioassay - calcium flux assay and activation of CXCR4Product: Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a Protein Catalog # 350-NSReference(s):Geeraerts, T. et al. (2006) Effects of SDF-1a and gp120IIIB on apoptotic pathways in SK-N-SH neuroblastoma cells. Neurosci. Lett. 399:115.Sample Type: human SK-N-SH neuroblastoma cell line

Application: ImmunohistochemistryProduct: Human CXCL12/SDF-1 Polyclonal Antibody Catalog # AF-310-NAReference(s):Corcione, A. et al. (2004) Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis. Proc. Natl. Acad. Sci. USA 101:11064.Sample Type: human brain (multiple sclerosis active lesions)

CXCL13/BLC/BCA-1Application: ImmunohistochemistryProduct: Mouse CXCL13/BLC/BCA-1 Polyclonal Antibody Catalog # AF470Reference(s):Magliozzi, R. et al. (2004) Intracerebral expression of CXCL13 and BAFF is accompanied by formation of lymphoid follicle-like structures in the meninges of mice with relapsing experimental autoim-mune encephalomyelitis. J. Neuroimmunol. 148:11.Sample Type: mouse brain

Application: Western BlotProduct: Mouse CXCL13/BLC/BCA-1 Polyclonal Antibody Catalog # AF470Reference(s):Bagaeva, L.V. et al. (2006) CXC chemokine ligand 13 plays a role in experimental autoimmune encephalomyelitis. J. Immunol. 176:7676.Sample Type: mouse spinal cord homogenate

IFN-gApplication: Bioassay - oligodendrogenesisProduct: Recombinant Mouse IFN-g Protein Catalog # 485-MIReference(s):Butovsky, O. et al. (2006) Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis. J. Clin. Invest. 116:905.Sample Type: mouse microglia/neural progenitor cell co-culture

Application: Bioassay - cell cultureProduct: Recombinant Mouse IFN-g Protein Catalog # 485-MIReference(s):Chitnis, T. et al. (2007) Elevated neuronal expression of CD200 protects Wlds mice from inflamma-tion-mediated neurodegeneration. Am. J. Pathol. 170: 1695.Sample Type: mouse microglia and neuronal cells

Application: ELISA KitProduct: Rat IFN-g DuoSet Catalog # DY585Reference(s):Maher, F.O. et al. (2006) Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation. J. Neurochem. 96:1560.Sample Type: rat hippocampal and cortical homogenates, and glial cell supernatant

Application: In VivoProduct: Recombinant Mouse IFN-g Protein Catalog # 485-MIReference(s):Blais, V. & S. Rivest (2004) Effects of TNF-a and IFN-g on nitric oxide-induced neurotoxicity in the mouse brain. J. Immunol. 172:7043.Sample Type: mouse

3� For research use only. Not for use in diagnostic procedures.


IL-1 b/IL-1F2Application: ELISA DevelopmentProduct: Rat IL-1 b/IL-1F2 Biotinylated Polyclonal Antibody Catalog # BAF501Reference(s):Maher, F.O. et al. (2005) Downregulation of IL-4-induced signaling in hippocampus contributes to deficits in LTP in the aged rat. Neurobiol. Aging 26:717.Sample Type: rat brain homogenate

Application: ELISA KitProduct: Rat IL-1 b/IL-1F2 DuoSet Catalog # DY501Reference(s):Fernandes, A. et al. (2007) MAPKs are key players in mediating cytokine release and cell death induced by unconjugated bilirubin in cultured rat cortical astrocytes. Eur. J. Neurosci. 25:1058.Sample Type: rat astrocyte supernatant

Product: Rat IL-1 b/IL-1F2 Quantikine ELISA Kit Catalog # RLB00Reference(s):Blandino, P., Jr. et al. (2006) The involvement of norepinephrine and microglia in hypothalamic and splenic IL-1b responses to stress. J. Neuroimmunol. 173:87.Sample Type: rat hypothalamus and spleen homogenate

Adibhatla, R.M. & J.F. Hatcher (2007) Secretory phospholipase A2 IIA is up-regulated by TNF-a and IL-1a/b after transient focal cerebral ischemia in rat. Brain Res. 1134:199.Sample Type: rat brain homogenate

Application: ImmunohistochemistryProduct: Rat IL-1 b/IL-1F2 Polyclonal Antibody Catalog # AF-501-NAReference(s):Zawadzka, M. & B. Kaminska (2005) A novel mechanism of FK506-mediated neuroprotection: downregulation of cytokine expression in glial cells. Glia 49:36.Sample Type: rat brain

Kitaoka, Y. et al. (2007) NMDA-induced interleukin-1b expression is mediated by nuclear factor-k Bp65 in the retina. Brain Res. 1142:247.Sample Type: rat retina

Application: Multiplex assayProduct: Mouse IL-1 b/IL-1F2 Fluorokine MAP Catalog # LUM401Reference(s):Meyer, U. et al. (2006) The time of prenatal immune challenge determines the specificity of inflam-mation-mediated brain and behavioral pathology. J. Neurosci. 26:4752.Sample Type: mouse serum and fetal brain homogenate

Application: Western BlotProduct: Rat IL-1 b/IL-1F2 Polyclonal Antibody Catalog # AF-501-NAReference(s):Park, E.M. et al. (2005) Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression. Neuroscience 132:625.Sample Type: rat brain homogenate


Pleasevisitwww.RnDSytems.comforcompletelistingofinflammation-relatedmolecules.






ELISA/ASSAYS

NEUROINFLAMMATION

CCL�/MCP-�* HMCaCR HMCa HMCa HMRCa HMCa

CCL5/RANTES* HM HMCRF HMCR HMCRF HM

CXCL8/IL-8* HPCaF HPCaF HPCa HPCaF HPCa

CXCL��/SDF-�* HM H HM HMFRM HM

CX3CL�/Fractalkine HMR HMR HMR HMR HMR

CCR�* H H

CCR5* H H

CXCR�/IL-8RA H H

CXCR�/IL-8RB HM HM

CXCR3 HM HM

CXCR4 HMF HM

HMGB� H H

IFN-g HMRPBCaCR

HMRPBCaCREFPr

HMRPBCaCRFPr

HMRPBCaCREFPr

HMRPCaCRFPr

IL-�b/IL-�F�✦ HMRP HMRPCaCRF

HMRPCRF HMRPCaCREFPr

HMRPF

IL-�R3/IL-�RAcP H H H

IL-�RI,IL-�RII HM HM HM HM H

IL-�ra/IL-�F3 HMP HMPE HMP HMRPEcE HM

IL-�✦ HMRPCR HMRPBCaCREF

HMRPBCaCREF

HMRPBCaCREF

HMR

IL-�Ra HM HM HM HM HM

IL-�Rb HM HM HM H

IL-6✦ HMRPCaCRF

HMRPCaCREF

HMRPCaCRE

HMRPCaCREF

HMRPCaF

IL-6R HM HM HM HM HM

IL-�0✦ HMRPCaCRFV

HMRPCaCREFV

HMRPCaCRFV

HMRPCaCREFV

HMRPCaF

IL-�0Ra HM HM HM HM

IL-�0Rb H H H H

IL-�8/IL-�F4 HMRCa HRPCaPr HMRP HMRPPr HM

IL-�8Ra/IL-�R5 HM HM HM H

IL-�8Rb/IL-�R7 H HM HM H

TNF-a/TNFSF�A HMRPCaCRPr

HMRPBCaCREPr

HMRPBCaCRPr

HMRPBCaCREFPr

HMRPCaEPr

TNFRI/TNFRSF�A HM HM HM HMCa HM

TNFRII/TNFRSF�B HM HM HM HM HM

*Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/Chemokineforcompletelisting.✦Additionalfamilymembersavailable.Pleasevisitwww.RnDSystems.com/go/Cytokinesforcompletelisting.



IL-1 RIApplication: Western BlotProduct: Mouse IL-1 RI Monoclonal Antibody Catalog # MAB7712Reference(s):Davis, C.N. et al. (2006) MyD88-dependent and -independent signaling by IL-1 in neurons probed by bifunctional Toll/IL-1 receptor domain/BB-loop mimetics. Proc. Natl. Acad. Sci. USA 103:2953.Sample Type: mouse neuron and glial cell lysate

IL-1ra/IL-1F3Application: ELISA KitProduct: Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit Catalog # MRA00Reference(s):Blain, J.F. et al. (2006) A deficit in astroglial organization causes the impaired reactive sprouting in human apolipoprotein E4 targeted replacement mice. Neurobiol. Dis. 21:505.Sample Type: mouse hippocampal homogenate

Application: ImmunohistochemistryProduct: Mouse IL-1ra/IL-1F3 Polyclonal Antibody Catalog # AF-480-NAReference(s):Park, E.M. et al. (2005) Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression. Neuroscience 132:625.Sample Type: rat brain

IL-4Application: Bioassay - differentiationProduct: Recombinant Mouse IL-4 Protein Catalog # 404-MLReference(s):Butovsky, O. et al. (2006) Microglia activated by IL-4 or IFN-g differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells. Mol. Cell. Neurosci. 31:149.Sample Type: mouse microglia and neural progenitor cell co-culture

Application: ELISA DevelopmentProduct: Rat IL-4 Monoclonal Antibody Catalog # MAB504Reference(s):Maher, F.O. et al. (2005) Downregulation of IL-4-induced signaling in hippocampus contributes to deficits in LTP in the aged rat. Neurobiol. Aging 26:717.Sample Type: rat brain homogenate

IL-6Application: ELISA KitProduct: Mouse IL-6 DuoSet Catalog # DY406Reference(s):Dimayuga, F.O. et al. (2007) SOD1 overexpression alters ROS production and reduces neurotoxic inflammatory signaling in microglial cells. J. Neuroimmunol. 182:89.Sample Type: mouse N9 microglial cell line supernatant

Product: Mouse IL-6 Quantikine ELISA Kit Catalog # M6000BReference(s):Hakkoum, D. et al. (2007) Interleukin-6 promotes sprouting and functional recovery in lesioned organotypic hippocampal slice cultures. J. Neurochem. 100:747.Sample Type: mouse organotypic hippocampal slice culture supernatant

Product: Rat IL-6 DuoSet Catalog # DY506Reference(s):Fernandes, A. et al. (2006) Inflammatory signaling pathways involved in astroglial activation by unconjugated bilirubin. J. Neurochem. 96:1667.Sample Type: rat astrocyte supernatant

IL-6 RApplication: ELISA KitProduct: Human IL-6 sR Quantikine ELISA Kit Catalog # DR600Reference(s):Aronica, E. et al. (2005) Activation of metabotropic glutamate receptor 3 enhances interleukin (IL)-1b-stimulated release of IL-6 in cultured human astrocytes. Neuroscience 130:927.Sample Type: human astrocyte cell culture supernatant

IL-10Application: ELISA KitProduct: Mouse IL-10 Quantikine ELISA Kit Catalog # M1000Reference(s):Chang, C.P. et al. (2007) The flavonoid baicalin protects against cerebrovascular dysfunction and brain inflammation in experimental heatstroke. Neuropharmacology 52:1024.Sample Type: mouse hypothalamus homogenate

Application: Multiplex assayProduct: Mouse IL-10 Fluorokine MAP Catalog # LUM417Reference(s):Meyer, U. et al. (2006) The time of prenatal immune challenge determines the specificity of inflam-mation-mediated brain and behavioral pathology. J. Neurosci. 26:4752.Sample Type: mouse serum and fetal brain homogenate



IL-18/IL-1F4Application: Bioassay - inhibition of long-term potentiationProduct: Recombinant Rat IL-18/IL-1F4 Protein Catalog # 521-RLReference(s):Cumiskey, D. et al. (2007) Interleukin-18 mediated inhibition of LTP in the rat dentate gyrus is at-tenuated in the presence of mGluR antagonists. Neurosci. Lett. 412:206.Sample Type: rat hippocampus

Application: In VivoProduct: Recombinant Human IL-18 BPa/Fc Chimeric Protein Catalog # 119-BPReference(s):Felderhoff-Mueser, U. et al. (2005) Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain. Ann. Neurol. 57:50.Sample Type: rat

Application: Western BlotProduct: Rat IL-18/IL-1F4 Polyclonal Antibody Catalog # AF521Reference(s):Hedtjarn, M. et al. (2002) Interleukin-18 involvement in hypoxic-ischemic brain injury. J. Neurosci. 22:5910.Sample Type: rat brain homogenate

IL-18 Ra/IL-1 R5 Application: ImmunohistochemistryProduct: Mouse IL-18 Ra/IL-1 R5 Polyclonal Antibody Catalog # AF856Reference(s):Hedtjarn, M. et al. (2002) Interleukin-18 involvement in hypoxic-ischemic brain injury. J. Neurosci. 22:5910.Sample Type: rat brain

Application: Western BlotProduct: Human IL-18 Ra/IL-1 R5 Polyclonal Antibody Catalog # AF840Reference(s):Felderhoff-Mueser, U. et al. (2005) Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain. Ann. Neurol. 57:50.Sample Type: rat brain homogenate

IL-23Application: ImmunohistochemistryProduct: Mouse IL-23 p19 Polyclonal Antibody Catalog # AF1619Reference(s):Kel, J. et al. (2007) Soluble mannosylated myelin peptide inhibits the encephalitogenicity of autore-active T cells during experimental autoimmune encephalomyelitis. Am. J. Pathol. 170:272.Sample Type: mouse brain and spinal cord

IL-28AApplication: Bioassay - proliferation and STAT phosphorylationProduct: Recombinant Human IL-28A/IFN-l 2 Protein Catalog # 1587-ILReference(s):Zitzmann, K. et al. (2006) Novel interferon-lambdas induce antiproliferative effects in neuroendo-crine tumor cells. Biochem. Biophys. Res. Commun. 344:1334.Sample Type: human BDN1 pancreatic neuroendcrine tumor cell line

MOGApplication: Western blotProduct: Mouse MOG Polyclonal Antibody Catalog # AF2439Reference(s):Miyake, Y. et al. (2007) Critical role of macrophages in the marginal zone in the suppression of im-mune responses to apoptotic cell-associated antigens. J. Clin. Invest. 117:2268.Sample Type: MOG-transfected W3 cell lysates


MOG Expression in Mouse Cortex. MOG was detected in a cryostat section of mouse cortex using anti-mouse MOG polyclonal antibody (Catalog # AF2439). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008) and counterstained with hematoxylin.

NT-3Application: ELISA to measure release rate of NT-3 from fibrin gelsProduct: Human NT-3 DuoSet Catalog # DY267Reference(s):Taylor, S.J. et al. (2004) Controlled release of neurotrophin-3 from fibrin gels for spinal cord injury. J. Control Release 98:281.Sample Type: recombinant human NT-3 protein




OsteopontinApplication: ImmunohistochemistryProduct: Mouse Osteopontin Polyclonal Antibody Catalog # AF808Reference(s):Selvaraju, R. et al. (2004) Osteopontin is upregulated during in vivo demyelination and remyelina-tion and enhances myelin formation in vitro. Mol. Cell. Neurosci. 25:707.Sample Type: mouse brain

PARK-7/DJ-1Application: Immunohistochemsitry

PARK-7/DJ-1 Expression in Human Brain. PARK-7/DJ-1 was detected in paraffin-embedded human brain (hypothalamus) tissue sections using anti-human PARK-7/DJ-1 polyclonal anti-body (Catalog # AF3668). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Stain-ing Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue).

RAGEApplication: ImmunohistochemistryProduct: Human RAGE Polyclonal Antibody Catalog # AF1145Reference(s):Tezel, G. et al. (2007) Accelerated aging in glaucoma: immunohistochemical assessment of advanced glycation end products in the human retina and optic nerve head. Invest. Ophthalmol. Vis. Sci. 48:1201.Sample Type: human eye

RAGE Expression in Alzheimer’s Human Brain. RAGE was detected in paraffin-embedded Alzheimer’s human cerebellum sections using anti-human RAGE polyclonal antibody (Catalog # AF1145). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue)

a-SecretaseApplication: Bioassay - enzyme activityProduct: a-Secretase Activity Kit Catalog # FP001Reference(s):Hwang, E.M. et al. (2006) Furin is an endogenous regulator of a-secretase associated APP process-ing. Biochem. Biophys. Res. Commun. 349:654.Sample Type: mouse brain cortex homogenate

Wang, J. et al. (2006) Moderate consumption of Cabernet Sauvignon attenuates Ab neuropathol-ogy in a mouse model of Alzheimer’s disease. FASEB J. 20:2313.Sample Type: mouse neocortex homogenate and neuron lysate

b-SecretaseApplication: Bioassay - enzyme activityProduct: b-Secretase Activity Kit Catalog # FP002Reference(s):Hutter-Paier, B. et al. (2004) The ACAT inhibitor CP-113,818 markedly reduces amyloid pathology in a mouse model of Alzheimer’s disease. Neuron 44:227.Sample Type: human brain homogenate and recombinant human BACE-1 protein

Sastre, M. et al. (2003) Nonsteroidal anti-inflammatory drugs and peroxisome proliferator-activated receptor-g agonists modulate immunostimulated processing of amyloid precursor protein through regulation of b-secretase. J. Neurosci. 23:9796.Sample Type: mouse N2a and human SK-N-SH neuroblastoma cell line lysates

Tan, J. et al. (2002) Role of CD40 ligand in amyloidosis in transgenic Alzheimer’s mice. Nat. Neurosci. 5:1288.Sample Type: mouse brain homogenate

Tyler, S.J. et al. (2002) a- and b-secretase: profound changes in Alzheimer’s disease. Biochem. Biophys. Res. Commun. 299:373.Sample Type: human temporal cortex homogenate

g-SecretaseApplication: Bioassay - enzyme activityProduct: g-Secretase Activity Kit Catalog # FP003Reference(s):Burns, M. et al. (2003) Presenilin redistribution associated with aberrant cholesterol transport enhances b-amyloid production in vivo. J. Neurosci. 23:5645.Sample Type: mouse brain homogenate

Wang, J. et al. (2006) Moderate consumption of Cabernet Sauvignon attenuates Ab neuropathology in a mouse model of Alzheimer’s disease. FASEB J. 20:2313.Sample Type: mouse neocortex homogenate and neuronal lysate



TauApplication: Immunohistochemistry

Tau Expression in Alzheimer’s Human Brain (Cortex). Tau was detected in paraffin-embed-ded Alzheimer’s human brain (cortex) tissue sections using anti-human Tau polyclonal anti-body (Catalog # AF3494). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Stain-ing Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

TNF-a/TNFSF1AApplication: ImmunohistochemistryProduct: Rat TNF-a/TNFSF1A Polyclonal Antibody Catalog # AF-510-NAReference(s):Leonoudakis, D. et al. (2004) TNF-a-induced AMPA-receptor trafficking in CNS neurons: relevance to excitotoxicity? Neuron Glia Biol. 1:263.Sample Type: rat astrocytes

Application: Multiplex assayProduct: Mouse TNF-a/TNFSF1A Fluorokine MAP Catalog # LUM410Reference(s):Meyer, U. et al. (2006) The time of prenatal immune challenge determines the specificity of inflam-mation-mediated brain and behavioral pathology. J. Neurosci. 26:4752.Sample Type: mouse serum and fetal brain homogenate

Application: Western BlotProduct: Mouse TNF-a/TNFSF1A Monoclonal Antibody Catalog # MAB410Reference(s):Figiel, I. & K. Dzwonek (2007) TNF-a and TNF receptor 1 expression in the mixed neuronal-glial cultures of hippocampal dentate gyrus exposed to glutamate or trimethyltin. Brain Res. 1131:17.Sample Type: rat hippocampal dentate gyrus homogenate

TNF-b/TNFSF1BApplication: ImmunohistochemistryProduct: Human TNF-b/TNFSF1B Polyclonal Antibody Catalog # AF-211-NAReference(s):Corcione, A. et al. (2004) Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis. Proc. Natl. Acad. Sci. USA 101:11064.Sample Type: human brain

TNF RI/TNFRSF1AApplication: ELISAProduct: Human TNF RI/TNFRSF1A Quantikine ELISA Kit Catalog # DRT100Reference(s):Krabbe, K.S. et al. (2005) Low-dose endotoxemia and human neuropsychological functions. Brain. Behav. Immun. 19:453.Sample Type: human EDTA plasma

TRAIL/TNFSF10Application: Bioassay - cell viabilityProduct: Recombinant Human TRAIL/TNFSF10 Protein Catalog # 375-TLReference(s):Song, J.H. et al. (2006) Human astrocytes are resistant to Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis. J. Neurosci. 26:3299.Sample Type: human astrocytes and U343MG glioma cell line

Application: ImmunohistochemistryProduct: Human TRAIL/TNFSF10 Polyclonal Antibody Catalog # AF375Reference(s):Ryan, L.A. et al. (2004) TNF-related apoptosis-inducing ligand mediates human neuronal apoptosis: links to HIV-1-associated dementia. J. Neuroimmunol. 148:127.

Sample Type: human brain

TRAIL R2/TNFRSF10BApplication: Flow CytometryProduct: Human TRAIL R2/TNFRSF10B Polyclonal Antibody Catalog # AF631Reference(s):Ricci-Vitiani, L. et al. (2004) Absence of caspase 8 and high expression of PED protect primitive neural cells from cell death. J. Exp. Med. 200:1257.Sample Type: human neural progenitor cells




R&D Systems TOOLS FOR CELL BIOLOGY RESEARCH

R&D Systems is a leading supplier of cell biology reagents with over 30 years of experience manufacturing specialty biological products that serve the basic research, clinical research, and clinical diagnostic markets. We offer products for the study of cancer, developmental biology, endocrinology, glycobiology, immunology, neuroscience, proteases, signal transduction, and stem cell research.

About Us

R&D Systems offers more than 250 complete kits and development reagents for the measurement of cytokines, growth factors, adhesion molecules, eicosanoids, hormones, signal transduction molecules, and more. Complete kits are fully validated, require no development time, and are quality assured to meet the performance criteria outlined in each product manual or package insert. Development reagents contain matched antibodies and other components required for development of a working assay by the customer.

ELISAs

Complete Kits

Quantikine® Colorimetric sandwich ELISAs

Quantikine HS High sensitivity colorimetric sandwich ELISAs

Quantikine IVD® Colorimetric immunoassays for in vitro diagnostic use

QuantiGlo®Chemiluminescent ELISAs

Parameter™Small molecule microplate-based competitive assays

Surveyor™ IC (Intracellular)Intracellular protein ELISAs

Cell-Based ELISAsFluorescence assays to permit screening of intracellular proteins using whole cells without the need to prepare cell lysates.

Development Reagents

DuoSet® ELISA Development SystemsReagents for the development of ELISAs for cytokines, growth factors, and related molecules

DuoSet IC (Intracellular) ELISA Development SystemsReagents for the development of ELISAs for intracellular factors involved in apoptosis and signal transduction

Matched Antibody Pairs for ELISA DevelopmentDesign your own ELISA using our matched capture and detection antibody pairs

More than 1,500 recombinant and natural proteins from 15 different species are available from R&D Systems. Stringent production and purification standards, as well as rigorous bioassay or functional ELISA activity testing, ensure quality and minimize lot-to-lot variability.

Recombinant & Natural Proteins

• Cytokines • Chemokines • Growth Factors • Receptors • Proteases • Adhesion Molecules • Neurotrophic Factors • Developmental Molecules • DNA Modifying Enzymes • Kinases, Phosphatases, & Sulfatases • Inhibitors & more

Features• Typically >95% purity • Low endotoxin level • High biological activity • Long-term stability • Preservative-free • Available with BSA or carrier-free • Available in bulk quantities

All package inserts are available on our website at www.RnDSystems.com



Tools for the expansion and analysis of neural, mesenchymal, hematopoietic, and embryonic stem cells are available from R&D Systems. Serum-free and serum-containing media, as well as extracellular matrix-related reagents are also available.

Stem Cell & Cell Culture/ Selection

Stem Cell Kits• Expansion Kits • Functional Identification Kits • Differentiation Kits • Marker Antibody Panels

Primary Cells• Rat Cortical Stem Cells

Culture Media & Supplements• StemXVivo™ culture media• Methylcellulose-based reagents for colony

forming cell assays

Cell Selection Kits• Cell enrichment columns for T cell subsets

& eosinophils• MagCellect™ Kits for T cell subsets, B cells, progenitors

& lineage depletion

Molecular Biology Reagents• Primer Pairs

Extracellular Matrix Reagents• Basement membrane extracts & cell invasion assays• Individual extracellular matrix molecules• Extracellular matrix-coated plates

R&D Systems has developed both antibody-spotted membrane-based arrays and bead-based assays for the measurement of levels and/or phosphorylation status of multiple analytes in a single sample.

Multiplex Assays/Arrays

Proteome Profiler™ ArraysMembrane-based arrays for cytokines, immunorecep-tors, receptor tyrosine kinases, intracellular kinases, & more

Fluorokine® MAP (Multi-analyte Profiling) for the Luminex® PlatformValidated panels of bead sets configured for cytokines, MMPs, adhesion molecules, and obesity markers

R&D Systems ELISpot and cell-based assays for cytokine secretion, intracellular signaling, apoptosis, and recep-tor binding are ideal to observe the effects of test compounds such as growth factors, drugs, and inhibitors on specific cell populations.

ELISpot, Cell-Based Assays/Reagents, & Flow Cytometry Reagents

ELISpotHighly sensitive microplate-based assays to detect absolute numbers of cytokine , chemokine, or protease-secreting cells

ELISpot Development ModulesReagents for the development of ELISpot assays for cytokines, chemokines, proteases, & more

Cell-Based ELISAs Fluorescence assays to permit screening of intracellular proteins using whole cells without the need to prepare cell lysates. These assays also offer the ability to measure total and phopho-proteins in the same well.

Flow Cytometry Kits & Reagents• Cell surface staining• Fluorokine® Kits (labeled cytokines used to monitor

the presence of cytokine receptors)• Intracellular staining

R&D Systems offers a variety of assays for the measurement of kinase, phosphatase, or protease activity, as well as transcription factor binding.

Activity Assays & Reagents

DuoSet IC (Intracellular) Assays & Development Systems• Kinase activity assays• Transcription factor activity assays• Phosphatase activity assays

Fluorokine ECapture antibody-based fluorometric enzyme assays for quantification of active MMPs

Apoptosis & Cell Viability Detection Kits• Caspase activity assays• Annexin V detection• Mitochondrial membrane potential disruption• DNA fragmentation detection kits• PARP activity assay• SOD activity assay• TUNEL assays• MTT & XTT Cell Proliferation/Viability Assay

MagCellect, Proteome Profiler, StemXVivo, and NorthernLights are trademarks of R&D Systems, Inc.DuoSet, Fluorokine, and Quantikine are registered trademarks of R&D Systems, Inc. R&D Systems is a registered trademark of TECHNE Corporation.



R&D Systems ANTIBODIES

HostSpecies

Mouse Donkey Hamster Rat

Chicken Goat Rabbit Sheep

TargetSpecies

Human Canine Equine T. gondii

Mouse Chicken Feline Viral

Rat CottonRat Porcine Xenopus

Bovine Drosophila Primate Zebrafish

Applications

WesternBlot MatchedAntibodyPairsforELISADevelopment

FlowCytometry(CellSurfaceorIntracellular) Immunoprecipitation

Immunohistochemistry/Immunocytochemistry CellSelection

Neutralization BlockadeofReceptor-LigandInteraction

AntibodyConjugates

Fluorescent(Excitation/Emission maxima) Others

NorthernLights-493(493/514) Biotin(Btn)

NorthernLights-557(557/575) AlkalinePhosphatase(AP)

NorthernLights-637(637/658) HorseradishPeroxidase(HRP)

Allophycocyanin(APC)(645/660) Cell&TissueStainingKits

Fluorescein(CFS)(492/517)

Phycoerythrin(PE)(565/575)

NorthernLightsANTIBODY/LABEL

CATALOG# Abs/EmMAXIMA

LASER(Ex) COMPARABLEFLUOROCHROMES

NorthernLights-493anti-RabbitIgGanti-MouseIgGanti-GoatIgGanti-SheepIgGanti-RatIgGStreptavidin

NL006NL009NL003NL0��NL0�5NL997

493/5�4 Argon(488)

FITC(49�/5�0)Cy�(489/506)AlexaFluor488(494/5�9)

NorthernLights-557 anti-RabbitIgGanti-MouseIgGanti-GoatIgGanti-SheepIgGanti-RatIgGStreptavidin

NL004NL007NL00�NL0�0NL0�3NL999

557/575 Krypton(568)HeNe(543)

Phycoerythrin(565/575)RhodamineRedX(570/590)Cy3(548/56�)

NorthernLights-637 anti-RabbitIgGanti-MouseIgGanti-GoatIgGanti-SheepIgGanti-RatIgGStreptavidin

NL005NL008NL00�NL0��NL0�4NL998

637/658 HeNe(633) Allophycocyanin(645/660)AlexaFluor647(650/668)Cy5(650/670)

R&DSystemsalsooffersAntibodyIsotypecontrols.Visitwww.RnDSystems.com/go/NorthernLightsformoreinformation.

With more than 7000 primary antibodies available, the addition of the NorthernLights line of fluorescent secondary antibodies makes R&D Systems your complete source for immunofluo-rescence reagents.

NorthernLights secondary antibodies are bright and fade-resistant, recognizing mouse, goat, sheep, rat, and rabbit IgG. Like all R&D Systems antibodies, NorthernLights antibody conju-gates are specific and deliver a high signal-to-noise ratio. Also available are streptavidin con-jugates for labeling biotinylated antibodies.

NorthernLights™ A new line of fluorochrome-conjugates for IHC & Flow Cytometry

Confocal Images of Triple Immunofluoresence in Mouse Brainstem. Cryostat sections of mouse brainstem were labeled with mouse anti-Orexin B monoclonal antibody (Catalog # MAB734), rabbit anti-human/mouse/rat p38a polyclonal antibody (Catalog # AF8691), and goat anti-mouse Integrin b1 (Catalog #AF2405). Tissue was stained with NorthernLights (NL)-conjugated secondary polyclonal antibodies: NL-493 donkey anti-rabbit (Catalog # NL006), NL-557 donkey anti-mouse (Catalog # NL007), and NL-637 donkey anti-goat (Catalog # NL002). Immunohistochemical image of integrin b1 expression is pseudo-colored for presentation.

Orexin B Integrin b1

p38a



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NOTES

r&d systems neuroscience reference guide for research use only. not for use in diagnostic...

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