Recall: recombinant DNA technology

The things that you need: The gene of interest (or its source)

The vector (e.g. plasmid)

The enzyme that digests the DNA Restriction enzyme

The enzyme that puts the DNA fragments together DNA ligase

Recall: restriction enzyme

Group activity

Per group, construct a recombinant DNA of the Bt gene in a plasmid with promoter and terminator elements Cut the plasmid, the Bt gene,

the promoter and terminator elements with the appropriate REs

Put all the elements together to make the recombinant construct