reagents for histological techniques, haematology … · reagents for histological techniques,...

Reagents forHistologicaltechniques,Haematologyand Microbiology

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PanReac AppliChem has a full range of products for histology, haematology and microbiology, which includes the most commonly used reagents in the process of preparing samples for examination under the microscope. With this range, all the stages of fixing, clearing, paraffin inclusion, staining and mounting are covered.

The majority of the products used in this technique are encompassed in the Clinical Diagnosis quality, with the CE mark in compliance with the provisions of the European Directive on products for “in vitro” diagnosis.

Reagents for Histological techniques, Haematology and Microbiology

Stages ofHistological Technique

1 The range includes the majority of reagents used for this purpose, starting with the classic and commonly used buffered formaldehyde, in a wide range of packaging formats. As an alternative to Formaldehyde we recommend using the new Histofix® Substitute of Formaldehyde in the technically viable applications, in order to minimize risks to the health of the user.Other fixing agents are also available for specific applications, such as Carnoy or Bouin Fixing Agents, all of which are manufactured with the most widely accepted formulas.

Fixing

2 Drying and ClearingThe range of solvents for the drying stage includes different types of alcohols, chiefly ethanol, in different, ready-to-use concentrations and other solvents used for this purpose. All are specially purified and subjected to stringent quality control, to prevent the presence of particles that could cause artefacts in the preparation of tissues. The range of clearing agents includes the classics: Xylene, Citrosol, and other solvents, together with Isoparaffin H, a Xylene surrogate, non-aromatic, practically odourless, and much less volatile than Xylene.

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3 InclusionParaffin inclusion is widely covered with paraffins with different melting points, that meet the majority of needs in this field.

4 StainingThe most commonly used stains are available in ready-to-use solutions, complemented by over 30 powdered dyes for preparation of any kind of stain with maximum flexibility. All the dyes are subjected to stringent quality control, to ensure maximum reproducibility in the preparation of the stains, preventing colour differences between different preparations. This is particularly important for prepared solutions, where the concentrations of dye or pH are specifically controlled, in order to ensure maximum contrast and homogeneous colour brightness lot after lot, with special emphasis on the different available formulations of hematoxylin-eosin and Papanicolau dyes for cytological studies.

The range includes different Kits: Gram staining in microbiology, Fast staining in haematology, Kit for staining Reticulin fibres, PAS staining and Masson’s Trichromic staining.

5MountingA wide range is available, from classic mounting media, Canada balsam and DPX, to the latest Mounting Medium for Xylene Surrogates, indicated for use in conjunction with Isoparaffin H. All of them with one purpose: to ensure perfect preservation of the preparations over time.

All in all, PanReac AppliChem offers you a complete range of products for pathological anatomy, with the maximum guarantee to ensure safe, reliable results in the histo-pathological process, thanks to the top quality and stringency of products designed specifically for this purpose.

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Description Code

Bouin Liquor Composition: Picric Acid moistenedwith ~33% H2O ...........................................1.125 gAcetic Acid glacial .............................................5 mlFormaldehyde 35-40% ................................ 25 mlWater................................................................... 77 ml

254102

Carnoy’s Fixing Composition: Acetic Acid glacial ........................................100 mlEthanol absolute ..........................................600 mlTrichloromethane stabilized

with ethanol ..................................................300 ml

251917

Ethanol absolute 251086

Ethanol 96% v/v 251085

Ethanol-Diethyl Ether 1:1 251084

Fixing B-5 Composition: Mercury(II) Chloride ........................................... 6 gSodium Acetate 3-hydrate ........................ 2.06 gWater s.q.m. ....................................................100 ml

253500

Formaldehyde 30-36% w/v concentrated buffered to pH=7 stabilized with methanol 253572

Formaldehyde 3.7-4.0% buffered to pH=7 and stabilized with methanol 252931

Histofix® Preservative ready to use 256462

Histofix® Substitute of Formaldehyde 255805

Histofix® Substitute of Formaldehyde ready for use 257157

Histofix® Spray fixative for fixing samples in Papanicolaou stain Composition: Polyethylene Glycol 6000 ..............................50 gWater................................................................... 75 mlEthanol s.q.m. ................................................925 ml Histofix® is a Registered Trademark of Panreac Química S.L.U.

256700

Embalming Mixture Composition: Phenol 90% ...................................................12.5 mlEthanol 96% .................................................62.5 mlFormaldehyde solution 35-40% ............. 7.5 mlGlycerol ...........................................................17.5 ml

214632

Fixing reagents for electronic microscopy

Glutaraldehyde solution 25% 253857

Osmium(VIII) Oxide 252901

Fixing AgentsFixing

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FixingDecalcifiers Inclusion paraffinsDescription Code

Histofix® marrow decalcifierBone marrow decalcification and fixation Composition:3x100 ml Solution A fixative3x100 ml Solution B decalcifier

256284

Histofix® decalcifier 1slow decalcifier and fixing agent 256239

Histofix® decalcifier 2medium decalcifier for fixed tissues

256238

Histofix® decalcifier 3fast decalcifier for fixed tissues 256237

Histofix® is a Registered Trademark of Panreac Química S.L.U.

Description Code



Ethanol 96% v/v partially denaturedtechnical grade 212800

Ethanol absolute 251086

Ethanol absolute partially denaturedtechnical grade 212801

Xylene, mixture of isomers 251769

Citrosol (Substitute of Xylene) 253139

Isoparaffin H (Substitute of Xylene) 255069

Toluene pure 141745

Drying and clearing agents

Description Code

Paraffin M.P. 51-53°Cpellets

253209

Paraffin M.P. 55-58°Cplasticized + DMSO pellets

256993

Paraffin M.P. 56-58°Cpellets

253211

Paraffin M.P. ~ 42-44°Cpieces technical gradelow melting point

213206

Auxiliary Reagent:

Paraffin Cleaner Microtomes cleaner used inthe processing of human tissueComposition:Isoparaffin H .............. 425 ml1-Propanol ................ 75 ml

256876

Inclusion

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StainingPowdered dyesDescription Code

Acridine Orange (C.I. 46005) fluorescent staining A1398

Alcian Blue 8 GX (C.I. 74240) Certified by Biological Stain Commission 254584

Amido Black 10 B (C.I. 20470) for electrophoresis A1399

Aniline Blue WS (C.I. 42755) collagen staining 253708

Azur II (C.I. 52010 + 52015) blood smears staining 251178

Brilliant Blue FCF (C.I.42090) A5564

Brilliant Cresyl Blue (C.I. 51010) platelets and thrombocytes staining 251169

Brilliant Green (C.I. 42040) vegetal tissue staining 251758

Bromophenol Blue proteins staining 131165

Bromothymol Blue vital staining 131167

Carmine (Lacquer of carminic acid with calcium and aluminium) (C.I. 75470)nucleus and glycogen staining

251824

Congo Red (C.I. 22120) bacteriology 121611

Coomassie Brilliant Blue G-250 (C.I. 42655) for electrophoresis A3480

Coomassie Brilliant Blue R-250 (C.I. 42660) for electrophoresis A1092

Crystal Violet (C.I. 42555) bacteria staining 251762

Eosin Bluish (C.I. 45400) vital staining and plasma staining A0821

Eosin Yellowish (C.I. 45380) vital staining and plasma staining 251299

Erythrosin B (C.I. 45430) 253982

Evans Blue (C.I. 23860) fluorescent staining A4388

Fuchsin Acid (C.I. 42685) blood smear staining 251331

Fuchsin Basic (C.I. 42510) nucleus and Koch's bacilli staining 251332

Gentian Violet (C.I. 42535 + 42555) bacteria staining according to Gram 251765

Giemsa's Azur-Eosin-Methylene Blue dye blood smears and protozoos staining 251337

Hematoxylin (C.I. 75290) vagina smear staining 251344

Indigo Carmine (C.I. 73015) nucleus and glycogen staining 251246

Leishman's Eosin-Methylene Blue dye blood smear staining 251377

Malachite Oxalate Green (C.I. 42000) cytoplasm of vegetal cells staining 251761

Methylene Blue (C.I. 52015) bacteriology and cytology 251170

Methyl Green (C.I. 42585) bacteria staining 251704

Methyl Violet (C.I. 42535) diptherial bacteria staining 252079

Neutral Red (C.I. 50040) Koch's bacilli staining 251619

Nuclear Fast Red (C.I. 60760) nucleus staining A8020

Orcein chromosome staining 251324

Ponceau S (C.I. 27195) for electrophoresis A1405

Pyronin Y (C.I. 45005) nucleic acid staining A1406

Resazurin for sterility tests 121591

Rhodamine B (C.I. 45170) fluorescent staining 251604

Rose Bengal (C.I. 45440) A4439

Safranine O (C.I. 50240) nucleus staining, according to Gram 251622

Sudan III (C.I. 26100) fatty acids and neutral fats staining in faeces 251731

Sudan Red G (C.I. 12150) 257122

Toluidine Blue O (C.I. 52040) nucleus and mucosae staining 251176

Trypan Blue (C.I. 23850) vital staining and connective tissue staining A0668

Wright's Eosin-Methylene Blue dye blood smear staining 251767

Xylene Cyanol FF (C.I. 42135) for electrophoresis A1408

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Description Code

Blue for fast staining (Panoptic No. 3) blood smear staining or medullary smear stainingComposition:Azur B ...................................................................... 2 gBuffer solution pH 7 s.q.m. ................... 1000 ml

253998

Carazzi’s Hematoxylin solution Solution for Hematoxylin-Eosin staining, in human

and gynaecological samplesComposition:Hematoxylin .......................................................0.1 gAluminium PotassiumSulfate 12-hydrate ............................................. 5 gSodium Iodate ................................................ 0.02 gGlycerol .............................................................. 20 mlWater s.q.m. ....................................................100 ml

255298

Eosin for fast staining (Panoptic No. 2) blood smear staining or medullary smear staining Composition:Eosin Yellowish .................................................0.8 gBuffer solution pH 7 s.q.m. ................... 1000 ml

253999

Eosin Yellowish alcoholic solution 1% Solution for Hematoxylin-Eosin staining, in humanand gynaecological samplesComposition:Eosin Yellowish .................................................10 gAcetic Acid glacial .............................................1 mlWater................................................................... 50 mlEthanol 96% ............................................... 1000 ml

256879

Eosin Yellowish hydroalcoholic solution 1% Solution for Hematoxylin-Eosin staining, in human andgynaecological samplesComposition:Eosin Yellowish .................................................... 1 gEthanol absolute ............................................ 10 mlWater................................................................... 90 ml

251301

Fixing for fast staining (Panoptic No. 1) blood smear staining or medullary smear staining Composition:Crystal Violet ..................................................... 2 mgMethanol s.q.m. ......................................... 1000 ml

254101

Gentian Violet Pheniquebacteria staining according to Gram-NicolleComposition:Gentian Violet ................................................. 0.67 gEthanol absolute ......................................... 11.7 mlPhenol ................................................................ 2.05 gWater.................................................................100 ml

251766

Giemsa’s Azur-Eosin-MethyleneBlue solution (slow) blood smears and protozoos staining Composition:Azur-Eosin-Methylene Blue dyeaccording to Giemsa .......................................0.5 gMethanol ........................................................... 50 mlGlycerol .............................................................. 50 ml

251338

Giemsa’s Azur-Eosin-MethyleneBlue, modified solution blood smear staining

257156

Gram-Hucker’s Crystal Violet Oxalate solution bacteria staining according to Gram-HuckerComposition:Crystal Violet .......................................................20 gAmmonium Oxalate ........................................... 8 gEthanol .............................................................200 mlWater.................................................................800 ml

252532

Description Code

Gram-Hucker’s Safranine O solution bacteria staining according to Gram-HuckerComposition:Safranine O ...................................................... 0.25 gEthanol absolute ............................................ 10 mlWater s.q.m. ....................................................100 ml

252531

Harris Hematoxylin solution Solution for Hematoxylin-Eosin staining, in human and gynaecological samplesComposition:Hematoxylin ................................................. 500 mgMercury(II) Oxide yellow ......................... 250 mgAluminium PotassiumSulfate 12-hydrate ...........................................10 gEthanol 96% .................................................... 16 mlWater................................................................... 88 ml

253949

Harris Hematoxylin modified solutionSolution for Hematoxylin-Eosin staining, in human andgynaecological samples. Mercury free.

256991

Kühne’s Methylene Blue Phenicated solution bacteria staining according to Ziehl-Neelsen, contrast dye Composition:Methylene Blue .................................................... 9 gEthanol absolute ............................................ 90 mlPhenol ................................................................. 26 mlWater.............................................................. 1000 ml

251172

Lactophenol Blue solution bacteriology Composition:Methyl Blue .....................................................50 mgPhenol ....................................................................25 gL(+)-Lactic Acid ............................................20.8 mlGlycerol ...........................................................39.5 mlWater s.q.m. ....................................................100 ml

253724

Light Green solution 0.1% animal tissue stainingComposition:Light Green SF yellowish...............................0.1 gWater s.q.m. ....................................................100 ml

253524

Löffler’s Methylene Blue Alkali solution Composition:Methylene Blue ............................................0.365 gPotassium Hydroxide 0.1 mol/l .............1.62 mlEthanol absolute ........................................... 9.1 mlWater................................................................... 91 ml

251171

Mayer’s Hematoxylin solutionfor cytologyComposition:Hematoxylin .......................................................1.0 gAluminium PotassiumSulfate 12-hydrate ...........................................50 gSodium Iodate ...................................................0.2 gChloral hydrate ..................................................50 gCitric Acid anhydrous ........................................ 1 gWater.............................................................. 1000 ml

254766

May Grünwald’s Eosin-Methylene Blue solutionblood smear stainingComposition:May Grünwald’s Eosin-MethyleneBlue dye ............................................................ 0.25 gMethanol s.q.m. ............................................100 ml

251416

Methyl Red solution 0.1%Composition:Methyl Red ............................................................ 1 gEthanol 70% ................................................ 1000 ml

281618

Orcein solution A hydroacetic-hydrochloric solution chromosome stainingComposition:Orcein ....................................................................2.0 gAcetic Acid .....................................................45.8 mlHydrochloric Acid 1 mol/l .......................... 8.3 mlWater................................................................45.8 ml

251993

Dyes in solution Dyes in solution

Staining Staining

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StainingDyes in solution

Description Code

Orcein solution B hydroacetic solution chromosome stainingComposition:Orcein ....................................................................2.0 gAcetic Acid ........................................................ 55 mlWater................................................................... 55 ml

251994

Papanicolaou’s Solution EA 50 for cytology Composition:Light Green SF yellowish............................58 mgBismarck Brown R ........................................40 mgEosin Yellowish ............................................0.225 gPhosphotungstic Acid hydrate ................ 0.17 gAcetic Acid glacial ............................................0.1 gWater......................................................................7 mlMethanol ........................................................... 93 ml

253594

Papanicolaou’s Solution OG 6 for cytologyComposition:Orange G..............................................................0.2 gPhosphotungstic Acid hydrate ................ 0.02 gEthanol absolute .........................................88.5 mlWater................................................................ 11.5 ml

253892

Schiff’s Reagent PAS staining for detection of carbohydrate Composition:Pararosaniline ....................................................0.1 gSodium Sulfite solution 10% .................... 10 mlHydrochloric Acid 35% ...................................3 mlWater................................................................... 50 ml

251588

Weigert’s Hematoxylin solution A nucleus staining Composition:Hematoxylin .......................................................... 1 gEthanol absolute ..........................................100 ml

253453

Weigert’s Hematoxylin solution B nucleus staining Composition:Iron(III) Chloride 30% aqueous solution .4 mlHydrochloric Acid 35% ...................................1 mlWater s.q.m. ....................................................100 ml

253454

Wright’s Eosin-Methylene Blue solution blood smear stainingComposition:Wright’s Eosin-Methylene Blue dye ...... 0.25 gMethanol s.q.m. ............................................100 ml

251768

Ziehl-Neelsen Carbol-Fuchsin Basic solution bacteria stainingaccording to Gram-Nicolle and Ziehl-Neelsen, contrast dyeComposition:Basic Fuchsin .................................................. 0.74 gPhenol ....................................................................5 mlEthanol absolute ............................................ 10 mlWater s.q.m. ....................................................100 ml

251333

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Description Code

Periodic Acid (Reag. USP, Ph. Eur.) for analysis, ACS 132320

Water for analysis, ACS 131074

Alcian Blue 8 GX (C.I. 74240) 254584

Schiff’s Reagent Composition:Pararosaniline ....................................................0.1 gSodium Sulfite solution 10% .................... 10 mlHydrochloric Acid 35% ...................................3 mlWater................................................................... 50 ml

251588

Hydrochloric Acid 1 mol/l (1N) 181021

Harris Hematoxylin solution Composition:Hematoxylin ................................................. 500 mgAluminium Potassium Sulfate 12-hydrate ..10 gMercury(II) Oxide yellow ......................... 250 mgEthanol 96% .................................................... 16 mlWater................................................................... 88 ml

253949

Sodium Disulfite for analysis 131698

Sample: Stomach. Method: PAS-Alcian Blue.Dr. Juan J. Jimenez and Dr. Isabel Hierro. Antequera Hospital. Malaga.

Alcian Blue-PAS Stainingcarbohydrates differentiation

Description Code


Toluidine Blue O (C.I. 52040) 251176

Sample: Lung cartilague. Method: Toluidine blue.Dr. Miquel Borràs - Experimental Toxicology and Ecotoxicology Unit .Parc Científic de Barcelona

Toluidine Blue StainingStaining of ganglion cells in mammalian viscera. Staining of fungi and mucus.

Description Code

Reticulin Kit Composition:Reagent A: KMnO4 (25 ml) Reagent B: Acide sol. (25 ml)Reagent C: H2C2O4 sol. (25 ml)Reagent D: NH4Fe(SO4)2 sol. (25 ml)Reagent E: AgNO3/NH4OH sol. (25 ml)Reagent F: HCHO sol. (25 ml)Reagent G: Na2S2O3 sol. (25 ml)

Sufficient for 50 tests

255115.0922

Sample: Small intestine. Method: Reticulin. General Hospital of Specialties“Ciudad de Jaen”. Pathological Anatomy Service. Jaén.

Reticulin Fiber Staining

StainingMost widely used stains

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Description Code



Lugol’s Liquor with 0.4% Iodine (diluted)Composition: Potassium Iodide..................0.66 gIodine...........................................0.4 gWater s.q.m...........................100 ml

251774

Lugol’s Liquor with 0.33% Iodine (diluted)Composition: Potassium Iodide.................0.66 gIodine.......................................0.33 gWater s.q.m..........................100 ml

256977

Lugol’s Liquor with 5% Iodine (concentrated) Composition: Potassium Iodide....................10 gIodine.............................................5 gWater s.q.m..........................100 ml

257041

Methanol (Reag. Ph. Eur.) for analysis, ACS, ISO

131091

Safranine O (C.I. 50240) 251622

Crystal Violet (C.I. 42555) 251762

Gram Stainingdifferentiation of Gram-positiveand Gram-negative bacteria

Description Code


Alcohol-Acetone 7:3 251803

Ziehl-Neelsen Carbol-Fuchsin Basic solution Composition: Basic Fuchsin....................0.74 gPhenol.....................................5 mlEthanol absolute..............10 mlWater s.q.m......................100 ml

251333

Lugol’s Liquor with 0.4% Iodine (diluted)Composition: Potassium Iodide..............0.66 gIodide.......................................0.4 gWater s.q.m.......................100 ml

251774

Lugol’s Liquor with 0.33% Iodine (diluted)Composition: Potassium Iodide..............0.66 gIodide....................................0.33 gWater s.q.m.......................100 ml

256977

Lugol’s Liquor with 5% Iodine (concentrated)Composition: Potassium Iodide..................10 gIodide...........................................5 gWater s.q.m.......................100 ml

257041

Gentian Violet Phenique Composition: Gentian Violet.....................0.67 gPhenol....................................2.05 gEthanol absolute..............11.7 mlWater.....................................100 ml

251766

Gram-Nicolle Stainingdifferentiation of Gram-positive and Gram-negative bacteria


Gram-Hucker Stainingdifferentiation of Gram-positive and Gram-negative bacteria

Description Code

Gram-Hucker’s Staining Kit (droppers) Composition:Alcohol-Acetone 7:3 (250 ml)Lugol’s Liquor (100 ml) Gram-Hucker’s Safranine O solution (100 ml)Gram-Hucker’s Crystal Violet Oxalate solution (100 ml)

256649.0922

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Sample: Skin. Method: May Grünwald-Giemsa.Dr. Mª Ángeles López and Dr. David Marcilla.Virgen del Rocío University Hospital. Sevilla

May-Grünwald-Giemsa or Pappenheim Stainingdifferentiation of blood cells

Description Code



Harris Hematoxylin solution Composition: Hematoxylin ................................................. 500 mg Aluminium Potassium Sulfate12-hydrate ...........................................................10 gMercury(II) Oxide yellow ......................... 250 mgEthanol 96% .................................................... 16 mlWater................................................................... 88 ml

253949

Papanicolaou’s Solution EA 50 Composition: Light Green SF yellowish............................58 mgBismarck Brown R ........................................40 mgEosin Yellowish ............................................0.225 gPhosphotungstic Acid hydrate ................ 0.17 gAcetic Acid glacial ............................................0.1 gWater......................................................................7 mlMethanol ........................................................... 93 ml

253594

Papanicolaou’s Solution OG 6 Composition: Orange G..............................................................0.2 gPhosphotungstic Acid hydrate ................ 0.02 gWater................................................................ 11.5 mlEthanol absolute .........................................88.5 ml

253892

Auxiliary Reagent:

Histofix® Spray fixative for fixing samples in Papanicolaou stain Composition: Polyethylene Glycol 6000 ..............................50 gWater................................................................... 75 mlEthanol s.q.m.. ...............................................925 ml

256700

Sample: Endometrium. Method: Papanicolaou.Dr. J.C. Morín. - Cytology Centre. Las Palmas G.C.

Papanicolaou Stainingearly detection of cervical or vaginal cancer

PAS Staining (Periodic Acid Schiff)carbohydrates detection


Description Code

PAS KitComposition: Reagent A: Periodic Acid (30 ml) Reagent B: Schiff’s Reagent (30 ml) Reagent C: Potassium metabisulfite sol. (30 ml) Reagent D: Fixing solution (30 ml) Reagent E: Mayer’s Hematoxylin (30 ml)


256676.0922

Description Code

Giemsa’s Azur-Eosin-Methylene Blue solution (slow) Composition: Azur-Eosin-Methylene Blue dyeaccording to Giemsa ........................................0.5 gMethanol ........................................................... 50 mlGlycerol .............................................................. 50 ml

251338

May Grünwald’s Eosin-Methylene Blue solution Composition: May Grünwald’s Eosin-MethyleneBlue dye ............................................................. 0.25 gMethanol s.q.m.. ...........................................100 ml

251416

Methanol (Reag. Ph. Eur.) for analysis, ACS, ISO 131091

Buffer Solution pH 7.2 Composition: Potassium di-Hydrogen Phosphate .......40 mgdi-Sodium Hydrogen Phosphate12-hydrate .................................................... 151 mgWater s.q.m. ....................................................100 ml

252164

Histofix® is a Registered Trademark of Panreac Química S.L.U.

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Description Code

Masson’s Trichrome Kit (with Aniline Blue)Composition:

Reagent A: Hematoxylin sol. B according to Weigert (30 ml)Reagent B: Hematoxylin sol. A according to Weigert (30 ml)Reagent C: Picric Acid alcoholic sol. (30 ml)Reagent D: Biebrich Scarlet sol. (30 ml)Reagent E: Phosphomolybdic Acid sol. (30 ml)Reagent F: Aniline Blue sol. (30 ml)


256692.0922

Masson’s Trichromic Stainingconnective tissue staining

Sample: GN Diffuse. Method: Masson’s Trichromic.Dr. Orradre Romeo. Complejo Hospitalario Virgen de la Salud. Servicio de Anatomía Patológica. Toledo.

Description Code

Kit for Fast Staining in Haematology (Fast Panoptic)Composition: Fixing for fast staining (Panoptic No. 1) (500 ml) Eosin for fast staining (Panoptic No. 2) (500 ml) Blue for fast staining (Panoptic No. 3) (500 ml)

254807.0922

Fast Staining in Haematology (Panoptic)blood or medullary smear staining

Sample: Peripheral blood swab. Method: Fast Kit (Panoptic). Dr. Verísimo Marta. Clinical Analysis Service, Grande Covian Medicine and Speciality Centre. Zaragoza.

Ziehl-Neelsen Staining acid-fast bacilli staining

Description Code


Alcohol-Hydrochloric 8:2 Composition: Hydrochloric Acid 35%.....................................20 ml

Ethanol absolute.........................................................80 ml251804

Alcohol-Hydrochloric (0.75 % HCl) 257097

Kühne’s Methylene Blue Composition: Phenicated solution Methylene Blue........................................................9 g Ethanol absolute.................................................90 ml Phenol.....................................................................26 ml Water s.q.m......................................................1000 ml

251172

Ziehl-Neelsen Carbol-Fuchsin Basic solution

Composition: Basic Fuchsin........................................................0.74 gPhenol.........................................................................5 mlEthanol absolute..................................................10 mlWater s.q.m..........................................................100 ml

251333

Sample Lung. Method: Ziehl-Neelsen. Dr. Mª Ángeles López and Dr. David Marcilla. Virgen del Rocío University Hospital. Sevilla.


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Description Code

Canada Balsam 251179

DPX, mounting medium fast (toluene base) 255254

Eukitt®, mounting medium 253681

Histofluid®, mounting medium 255598

Mounting Medium for substitutes of xylene 255811

Description Code

Cedarwood Oil A6586

Immersion Oil 251002

Immersion Oil purified 254561

Description Code

Benedict’s Reagent qualitative for determination of glucose in urine Composition: Sodium Carbonate anhydrous ...............153.6 gCitric Acid 1-hydrate ................................... 70.9 gCopper(II) Sulfate 5-hydrate .................... 15.6 gWater (s.q.m) ...........................................................1 l

251550

Biuret’s Reagent for determination of proteins Composition: Copper(II) Sulfate 5-hydrate .......................1.5 gSodium and PotassiumTartrate 4-hydrate ...........................................4.5 gPotassium Iodide ..............................................0.5 gSodium Hydroxide pellets .............................0.8 gWater (s.q.m) ..................................................100 ml

251820

Brij® 35 aqueous solution 30% w/vsurfactant for automatical analysis

252317

Collodion solution 4% w/v (USP) pure, pharma grade 141278

Copper(II) Sulfate solution d. 1.053for hematology, blood density

253296

Copper(II) Sulfate solution d. 1.055for hematology, blood density

253295

Fehling’s A Reagent for determination of glucose in urine and reducing sugars Composition: Copper(II) Sulfate 5-hydrate ..................48.30 gSulfuric Acid 96% .............................................1 mlWater (s.q.m) ...........................................................1 l

251563

Fehling’s B Reagent for determination of glucose in urine and reducing sugars Composition: Sodium Hydroxide ............................................90 gPotassium Sodium Tartrate 4-hydrate .. 300 gWater (s.q.m) ...........................................................1 l

251564

Description Code

Folin-Ciocalteu’s Reagent phenol reagent Composition: Sodium Tungstate 2-hydrate .......................10 gSodium Molybdate 2-hydrate ....................2.5 gortho-Phosphoric Acid 85% .........................5 mlHydrochloric Acid 35% ................................ 10 mlLithium Sulfate 1-hydrate .............................15 gWater s.q.m. ....................................................100 ml

251567

Isopentane for analysis 123501

Isopentane pure 143501

Kovacs’ Reagent for detection of indole in microbiology Composition: 4-(Dimethylamino) benzaldehyde..............50 gHydrochloric Acid 37% ..............................250 ml1-Butanol ........................................................750 ml

252908

Mercury(II) Nitrate 0.005 mol/l (0.01N)for determination of chloride, according to Schales and Schales

182138

Picric Acid saturated solutionfor determination of alcohol and creatinine

251049

Schiff’s ReagentComposition: Pararosaniline ....................................................0.1 gSodium Sulfite solution 10% .................... 10 mlHydrochloric Acid 35% ...................................3 mlWater................................................................... 50 ml

251588

Trichloroacetic Acid solution 20% w/vdeproteinizer

252373

Türck’s Liquorfor hematology, leucocytes Composition: Acetic Acid glacial ......................................... 1.5 mlMethylene Blue .............................................1.3 mgWater s.q.m. ....................................................100 ml

251588

Mounting media

MountingImmersion media

Other reagents (Haematology, Microbiology…)

14

“Laboratorio de Anatomía Patológica”,Raimundo García del Moral, 1ª Ed. McGraw-Hill – Interamericana de España, Madrid, 1993

“Laboratory Methods in Histotechnology”,American Registry of Pathology, Armed Forces Institute of Pathology, Washington D.C., 1992

“Staining Procedures”, Clark G., 4th Ed. Williams and Wilkins, Baltimore, 1981

“Manual Técnico de Análisis Clínicos”, E. Suárez Peregrin, 9ª Ed. Prieto, 1972

“Gradwoh’ls clinical laboratory methods and diagnosis”, S. Frankel, S. Reitman, A.C. Sonnenwirth, 7th Ed.The C.V. Mosby Company 2, 1970

“Lynch’s Medical Laboratory Technology”, Stanley S. Raphael, 3rd Ed. W.B. Saunders Company I, 1976

Bibliography

DERQUIM products have been specially made for cleaning the laboratory equipment. They can be used in the manual cleaning (series LM) or for washing using automatic machines (series LA). The various formulations are specifically adapted to any kind of laboratory residue: chemical, biological or clinical.The program includes DERQUIM DSF 11 for disinfecting surfaces and instruments.

Note: see Paraffin Cleaner in “Inclusion Paraffins” section

Because of the continuous updating of our catalog of products and presentations,kindly confirm product availability, through our website.

Description Code

For automatic or manual cleaning

DERQUIM + Universal Detergent, LIQUID 503574

For manual cleaning

DERQUIM LM 01 Alkaline LIQUID 502600

DERQUIM LM 02 Neutral, phosphates free LIQUID 502601

DERQUIM LM 03 Phosphates free LIQUID 502602

For automatic cleaning

DERQUIM LA 11 Slightly alkaline SOLID 502603

DERQUIM LA 12 Alkaline SOLID 502604

DERQUIM LA 13 Alkaline with detergents SOLID 502605

DERQUIM LA 14 Slightly alkaline LIQUID 502606

DERQUIM LA 15 Alkaline LIQUID 502607

DERQUIM LA 21 Acid, with phosphoric acid LIQUID 502608

DERQUIM LA 22 Acid, with citric acid LIQUID 502609

Auxiliary agent for automatic cleaning

DERQUIM SALT (Sodium Chloride lumps) 503468

Disinfectant

DERQUIM DSF 11 Antiseptic for surfaces and implements LIQUID 504994

Chromic mixture

DERQUIM MC Chromic mixture 502612

15

Description Code Package

Acridine Orange (C.I. 46005) A1398,0025 25 g b

Alcian Blue 8 GX(C.I. 74240)

254584.1604 5 g a

254584.1606 25 g a

Alcohol-Acetone 7:3 251803.1209 250 ml b

251803.1211 1000 ml b

Alcohol-Hydrochloric(0.75 % HCl)

257097.1211 1000 ml b

Alcohol-Hydrochloric 8:2 251804.1210 500 ml b

Amido Black 10 B (C.I. 20470)

A1399,0100 100 g b

Aniline Blue WS (C.I. 42755) 253708.1606 25 g a

Azur II (C.I. 52010 + 52015) 251178.1606 25 g a

Benedict's Reagent qualitative

251550.1211 1000 ml b

Biuret's Reagent 251820.1208 100 ml b

Blue for fast staining (Panoptic No. 3)

253998.1210 500 ml b

253998.1212 2.5 l b

Bouin Liquor 254102.1611 1000 ml a

Brij® 35 aqueous solution 30% w/v

252317.1611 1000 ml a

Brilliant Blue FCF (C.I.42090) A5564,0025 25 g b

Brilliant Cresyl Blue(C.I. 51010)

251169.1604 5 g a

Brilliant Green (C.I. 42040) 251758.1606 25 g a

251758.1608 100 g a

Bromophenol Blue 131165.1604 5 g a

131165.1606 25 g a

Bromothymol Blue 131167.1604 5 g a

131167.1606 25 g a

Buffer Solution pH 7.2 252164.1211 1000 ml b

Canada Balsam 251179.1608 100 ml a

251179.1609 250 ml a

251179.1611 1000 ml a

Carazzi's Hematoxylin solution

255298.1610 500 ml a

255298.1612 2.5 l a

Carmine (Lacquer of carminic acid with calcium and aluminium) (C.I. 75470)

251824.1605 10 g a

Carnoy's Fixing 251917.1611 1000 ml a

Cedarwood Oil A6586,0100 100 ml b

Citrosol (Substitute of Xylene)

253139.1611 1000 ml a

253139.1612 2.5 l a

253139.1214 5 l i

Collodion solution 4% w/v (USP) pure, pharma grade

141278.1609 250 ml a

141278.1611 1000 ml a


Congo Red (C.I. 22120) 121611.1605 10 g a

121611.1606 25 g a

Coomassie Brilliant Blue G-250 (C.I. 42655)

A3480,0010 10 g a

A3480,0025 25 g a

A3480,0100 100 g a

Coomassie Brilliant Blue R-250 (C.I. 42660)

A1092,0010 10 g b

A1092,0025 25 g b

A1092,0100 100 g b

Copper(II) Sulfate solution d. 1.053

253296.2711 1000 ml ~

Copper(II) Sulfate solution d. 1.055

253295.2711 1000 ml ~

Crystal Violet (C.I. 42555) 251762.1606 25 g a

DERQUIM + Universal Detergent, LIQUID

503574.1211 1000 ml b

503574.1214 5 l b

503574.1315 10 l C

DERQUIM DSF 11 Antiseptic for surfaces and implements LIQUID

504994.1214 5 l i

DERQUIM LA 11 Slightly alkaline SOLID

502603.1245 2 kg G

502603.0415 10 kg G

502603.0416 25 kg G

DERQUIM LA 12 Alkaline SOLID

502604.1245 2 kg G

502604.0415 10 kg G

502604.0416 25 kg G

DERQUIM LA 13 Alkaline with detergents SOLID

502605.1245 2 kg G

502605.0415 10 kg G

502605.0416 25 kg G

DERQUIM LA 14 Slightly alkaline LIQUID

502606.1214 5 l i

502606.0716 25 l i

DERQUIM LA 15 Alkaline LIQUID

502607.1214 5 l i

DERQUIM LA 21 Acid, with phosphoric acid LIQUID

502608.1214 5 l i

502608.1315 10 l C

DERQUIM LA 22 Acid, with citric acid LIQUID

502609.1214 5 l i

DERQUIM LM 01 Alkaline LIQUID

502600.1214 5 l i

DERQUIM LM 02 Neutral, phosphates free LIQUID

502601.1214 5 l i

502601.1315 10 l C

502601.0716 25 l i

DERQUIM LM 03 Phosphates free LIQUID

502602.1214 5 l i

DERQUIM MC Chromic Mixture

502612.2211 1000 ml 2

DERQUIM SALT (Sodium Chloride lumps)

503468.0415 10 kg G

503468.0416 25 kg G

Order Information

16


DPX, mounting medium fast (toluene base)

255254.1608 100 ml a

255254.1610 500 ml a

Embalming Mixture technical grade

214632.1214 5 l i

214632.0716 25 l i

Eosin Bluish (C.I. 45400) A0821,0100 100 g b

Eosin for fast staining (Panoptic No. 2)

253999.1210 500 ml b

253999.1212 2.5 l b

Eosin Yellowish (C.I. 45380) 251299.1606 25 g a

251299.1608 100 g a

Eosin Yellowish alcoholic solution 1%

256879.1210 500 ml b

256879.1612 2.5 l a

Eosin Yellowish hydroalcoholic solution 1%

251301.1609 250 ml a

251301.1611 1000 ml a

Erythrosin B (C.I. 45430) 253982.1606 25 g a

Ethanol 70% v/v 252695.1215 10 l i

Ethanol 96% v/v 251085.1211 1000 ml b

251085.1212 2.5 l b

251085.1214 5 l i

251085.1215 10 l i

251085.1315 10 l C

Ethanol 96% v/v partially denatured technical grade

212800.1211 1000 ml b

212800.1214 5 l i

212800.1315 10 l C

Ethanol absolute 251086.1211 1000 ml b

251086.1212 2.5 l b

251086.1214 5 l i

251086.1215 10 l i

251086.1315 10 l C

Ethanol absolute partially denatured technical grade

212801.1211 1000 ml b

212801.1214 5 l (1) i

212801.2814 5 l (2) i

212801.1315 10 l C

Ethanol-Diethyl Ether 1:1 251084.1610 500 ml a


Eukitt®, mounting medium 253681.0008 100 ml z

253681.0009 250 ml z

253681.0010 500 ml z

Evans Blue (C.I. 23860) A4388,0025 25 g b

Fehling's A Reagent 251563.1210 500 ml b

251563.1211 1000 ml b

251563.1212 2.5 l b

Fehling's B Reagent 251564.1210 500 ml b

251564.1211 1000 ml b

251564.1212 2.5 l b

Fixing B-5 253500.1211 1000 ml b

Fixing for fast staining (Panoptic No. 1)

254101.1210 500 ml b

254101.1212 2.5 l b

Folin-Ciocalteu's Reagent 251567.1609 250 ml a

Formaldehyde 3.7-4.0% buffered to pH=7 and stabilized with methanol

252931.0922 48x20 ml b

252931.1211 1000 ml b

252931.1212 2.5 l b

252931.1214 5 l i

252931.9914 5 l i

252931.1215 10 l i

252931.1315 10 l C

Formaldehyde 30-36% w/v concentrated buffered to pH=7 stabilized with methanol

253572.1211 1000 ml b

253572.1214 5 l i

Fuchsin Acid (C.I. 42685) 251331.1605 10 g a

Fuchsin Basic (C.I. 42510) 251332.1606 25 g a

251332.1608 100 g a

Gentian Violet(C.I. 42535 + 42555)

251765.1606 25 g a

251765.1609 250 g a

Gentian Violet Phenique 251766.1609 250 ml a

Giemsa's Azur-Eosin-Methylene Blue dye

251337.1608 100 g a

Giemsa's Azur-Eosin-Methylene Blue solution (slow)

251338.1608 100 ml a

251338.1610 500 ml a

251338.1611 1000 ml a

251338.1612 2.5 l a

Order Information

17


Giemsa’s Azur-Eosin-Methylene Blue, modified solution

257156.1610 500 ml a

257156.1611 1000 ml a

Glutaraldehyde solution 25%

253857.1611 1000 ml a

Gram-Hucker's Crystal Violet Oxalate solution

252532.1609 250 ml a

252532.1611 1000 ml a

Gram-Hucker's Safranine O solution

252531.1209 250 ml b

252531.1211 1000 ml b

Gram-Hucker's Staining Kit (droppers)

256649.0922 pack b

Harris Hematoxylin modified solution

256991.1610 500 ml a

256991.1612 2.5 l a

Harris Hematoxylin solution 253949.1610 500 ml a

253949.1611 1000 ml a

253949.1612 2.5 l a

Hematoxylin (C.I. 75290) 251344.1604 5 g a

251344.1606 25 g a

251344.1608 100 g a

Histofix® decalcifier 1 256239.1211 1000 ml b



Histofix® marrow decalcifier 256284.0922 pack b

Histofix® Preservativeready to use

256462.0905 45x10 ml b

256462.0955 44x20 ml b

256462.0962 45x30 ml b

256462.0961 45x40 ml b

256462.0967 24x75 ml b

256462.0943 16x125 ml b

256462.0944 12x200 ml b

256462.09149 10x600 ml b

256462.09118 1.5 l G

256462.0931 3 l G

Histofix® Spray fixative 256700.3408 6X100 ml b

Histofix® Substitute of Formaldehyde

255805.2711 1000 ml ~

255805.2714 5 l


Histofix® Substitute of Formaldehyde ready for use

257157.1211 1000 ml b

257157.1214 5 l i

Histofluid®, mounting medium

255598.0010 500 ml z

Hydrochloric Acid 1 mol/l (1N)

181021.1211 1000 ml b

181021.1212 2.5 l b

181021.1214 5 l b

181021.0715 10 l i

181021.1315 10 l C

Immersion Oil 251002.1207 50 ml(1) l

251002.1208 100 ml(1) l

251002.1209 250 ml b

Immersion Oil purified 254561.1208 100 ml b

Indigo Carmine (C.I. 73015) 251246.1605 10 g a

Isoparaffin H(Substitute of Xylene)

255069.2711 1000 ml ~

255069.2714 5 l

Isopentane for analysis 123501.1611 1000 ml a

Isopentane pure 143501.1611 1000 ml a

Kit for Fast Staining in Haematology (Fast Panoptic)

254807.0922 pack b

Kovacs' Reagent 252908.1608 100 ml a

Kühne's Methylene Blue Phenicated solution

251172.1208 100 ml b

251172.1209 250 ml b

251172.1211 1000 ml b

Lactophenol Blue solution 253724.1608 100 ml a

Leishman's Eosin-Methylene Blue dye

251377.1611 1000 g a

Light Green solution 0.1% 253524.1210 500 ml b

Löffler's Methylene Blue Alkali solution

251171.1208 100 ml a

251171.1209 250 ml a

Lugol's Liquor with 0.33% Iodine (diluted)

256977.1609 250 ml a

256977.1611 1000 ml a

Lugol's Liquor with 0.4% Iodine (diluted)

251774.1608 100 ml a

251774.1609 250 ml a

251774.1611 1000 ml a

Order Information

18


Lugol's Liquor with 5% Iodine (concentrated)

257041.1608 100 ml a

257041.1610 500 ml a

257041.1611 1000 ml a

Malachite Oxalate Green (C.I. 42000)

251761.1606 25 g a

251761.1608 100 g a

Masson's Trichrome Kit 256692.0922 pack b

Mayer's Hematoxylin solution

254766.1610 500 ml a

254766.1611 1000 ml a

May Grünwald's Eosin-Methylene Blue solution

251416.1610 500 ml a

251416.1611 1000 ml a

251416.1612 2.5 l a

Mercury(II) Nitrate 0.005 mol/l (0.01N)

182138.1611 1000 ml a

Methanol (Reag. Ph. Eur.) for analysis, ACS, ISO

131091.1211 1000 ml b

131091.1212 2.5 l b

131091.1214 5 l i

Methylene Blue (C.I. 52015) 251170.1606 25 g a

251170.1608 100 g a

251170.1609 250 g a

Methyl Green (C.I. 42585) 251704.1604 5 g a

251704.1606 25 g a

Methyl Red solution 0.1% 281618.1208 100 ml b

Methyl Violet (C.I. 42535) 252079.1208 100 g b

Mounting Medium for substitutes of xylene

255811.0008 100 ml z

Neutral Red (C.I. 50040) 251619.1605 10 g a

Nuclear Fast Red(C.I. 60760)

A8020,0005 5 g b

A8020,0025 25 g b

Orcein 251324.1604 5 g a

251324.1606 25 g a

Orcein solution A hydroacetic-hydrochloric solution

251993.1208 100 ml b

Orcein solution B hydroacetic solution

251994.1208 100 ml b

Osmium(VIII) Oxide 252901.0203 1 g k


Papanicolaou's Solution EA 50

253594.1610 500 ml a

253594.1611 1000 ml a

253594.1612 2.5 l a

Papanicolaou's Solution OG 6

253892.1610 500 ml a

253892.1611 1000 ml a

253892.1612 2.5 l a

Paraffin M.P. ~ 42-44°C pieces technical grade

213206.0911 1000 g 1

213206.0914 5 kg G

Paraffin M.P. 51-53°C pellets 253209.1211 1000 g b

Paraffin M.P. 55-58°C plasticized + DMSO pellets

256993.0933 6x1 kg 1

256993.0415 10 kg G

Paraffin M.P. 56-58°C pellets

253211.0914 5 kg 1

Paraffin Cleaner 256876.3408 6x100 ml b

256876.0922 15x100 ml b

PAS Kit 256676.0922 pack b

Periodic Acid (Reag. USP, Ph. Eur.) for analysis, ACS

132320.1608 100 g a

Picric Acid saturated solution

251049.1610 500 ml a

Ponceau S (C.I. 27195) A1405,0010 10 g b

A1405,0025 25 g b

A1405,0100 100 g b

Pyronin Y (C.I. 45005) A1406,0005 5 g b

Resazurin 121591.1604 5 g a

Reticulin Kit 255115.0922 pack a

Rhodamine B (C.I. 45170) 251604.1608 100 g a

Rose Bengal (C.I. 45440) A4439,0050 50 g a

Safranine O (C.I. 50240) 251622.1605 10 g a

251622.1607 50 g a

Schiff's Reagent 251588.1609 250 ml a

251588.1611 1000 ml a

Sodium Disulfite for analysis 131698.1210 500 g b

131698.1211 1000 g b

131698.1214 5 kg b

Order Information

19


Weigert's Hematoxylin solution A

253453.1210 500 ml b

Weigert's Hematoxylin solution B

253454.1210 500 ml b

Wright's Eosin-Methylene Blue dye

251767.1606 25 g a

Wright's Eosin-Methylene Blue solution

251768.1610 500 ml a

Xylene, mixture of isomers 251769.2711 1000 ml ~

251769.2712 2.5 l ~

251769.2714 5 l

Xylene Cyanol FF(C.I. 42135)

A1408,0010 10 g b

Ziehl-Neelsen Carbol-Fuchsin Basic solution

251333.1609 250 ml a

251333.1610 500 ml a

251333.1611 1000 ml a


Sudan III (C.I. 26100) 251731.1606 25 g a

251731.1608 100 g a

Sudan Red G (C.I. 12150) 257122.1605 10 g a

Toluene pure 141745.1611 1000 ml a

141745.1612 2.5 l a

141745.0314 5 l z

141745.3514 5 l

141745.0616 25 l s

Toluidine Blue O (C.I. 52040) 251176.1604 5 g a

251176.1606 25 g a

Trichloroacetic Acid solution 20% w/v

252373.1611 1000 ml a

Trypan Blue (C.I. 23850) A0668,0010 10 g b

A0668,0025 25 g b

Türck's Liquor 251390.1609 250 ml a

Water for analysis, ACS 131074.1211 1000 ml b

131074.1212 2.5 l b

131074.1214 5 l i

131074.1315 10 l C

131074.0716 25 l i

Order Information

(1) Bottle with dropper nozzle

Packaging symbols:

k Glass or plastic ampoule

s Steel-plated drum

Co-extrusion jerrycan (multilayer)

i Polyethylene canister

1 Carton box with inner polyethylene bag

l Polyethylene bottle with dropper

z Aluminium bottle

~ Co-extrusion bottle (multilayer)

b Polyethylene bottle

C Polyethylene container (cubitainer) with tap

a Glass bottle

2 Glass bottle coated with plastic

G Polypropylene bucket with handle

Fluorinated polyethylene canister

PanReac Química SLUC/ Garraf 2, Polígono Pla de la BrugueraE-08211 Castellar del Vallès (Barcelona) Spain Phone +34 937 489 400 Fax +34 937 489 [email protected] www.panreac.com

AppliChem GMbH Ottoweg 4DE-64291 DarmstadtGermany Phone +49 6151 9357 0Fax +49 6151 9357 [email protected]

Nova Chimica Srl Via G. Galilei, 47I-20092 Cinisello Balsamo (Milano) ItalyPhone +39 02 66045392Fax +39 02 [email protected]

A166,EN;201504

reagents for histological techniques, haematology … · reagents for histological techniques,...

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