realtimepcrexe

8/17/2019 realtimepcrexe

1/12

1

Biocompare, Inc . 395 Oyster Point Blvd., Suite 330 . South San Francisco, CA 94080 . (toll free)1.800.637.1277 . (p)650.873.9031 . (f)650.873.9038 . www.biocompare.com

C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A

l l R i g h t s R e s e r v e d

Real-Time PCR:Quantifying the qPCR Marketplace

Executive Summary


2/12


3/12

3


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


Methodology

The Real-Time PCR: Quantifying the qPCR Marketplace survey was administered on-line between August 23 and 27, 2004. Invitationsto complete the survey were emailed to life scientists who were interested in PCR technology. 746 researchers responded to theinvitation. 567 of these indicated that they were using or planning to use Real-Time PCR technology and completed the survey.

The survey consisted of 21 closed-ended questions. Eleven questions included “other” as a response option, with space providedfor the respondents to add the answer specific to their research.

Demographic information was evaluated from answers to 4 questions within the survey and from addresses provided by the

respondents.


4/12

4


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


Results: Demographics


5/12

5


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


92% of respondents were in North America or Europe.

Country

Asia

5%Europe

20%

North America

72%

Australia/New

Zealand2%South America

1%

n=567

*Only those respondents who are either currently using or planning to use real-time PCR are included in this chart.


6/12

6


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


The majority of respondents worked in academic institutions while 17% were in either Biotech orPharmaceutical companies and 10% worked in government labs.

In which type of institution do you work?

4%

4%

4%

6%

10%

13%

60%

0% 10% 20% 30% 40% 50% 60% 70%

Other

Clinical Diagnostic Testing

Pharmaceutical

Private Research

Government

Biotech

Academic

n=567



7/12

7


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


71.3% of respondents worked at the bench.

Which title best applies?

16.9%

15.2%14.8%

13.1%

11.3%

8.3%

8.1%2.5%

2.3%

1.9%

1.2%

0.7%0.7%

0.2%

2.8%

0.0% 2.0% 4.0% 6.0% 8.0% 10.0% 12.0% 14.0% 16.0% 18.0%

Postdoctoral Fellow

Staff ScientistPrincipal Investigator

Graduate Student

Technician/Research Assistant

Professor/Instructor

Lab Director/Chief ScientistProduct Manager

Research Director/VP of Research

Department Head

Business Development Director/Manager

ConsultantPresident/CEO/VP/Owner

Procurement manager

Other

n=567*Only those respondents who are either currently using or planning to use real-time PCR are included in this chart.


8/12

8


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


92% of respondents either authorized or recommended purchases.

Purchasing Authority

Authorize

43%

Evaluate

4%

Recommend

49%

No purchase role

4%

n=567



9/12

9


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


Many respondents identified multiple areas of research. Molecular Biology, Cell Biology, Genomics/Genetics andBiochemistry were each identified as a primary area of work by greater than one-third of survey respondents.

Primary area of Research or Work

4.9%

3.0%

3.2%

3.7%

6.5%

10.8%

11.3%

12.7%

12.9%

14.6%

14.8%

24.0%

24.0%

35.1%39.0%

50.6%

77.6%

0.0% 10.0% 20.0% 30.0% 40.0% 50.0% 60.0% 70.0% 80.0% 90.0%

None of the Above/Other

Marketing/Sales

Administration

Purchasing

Bioengineering

Drug Discovery

Pharmacology/Toxicology

Bioinformatics

Neuroscience

Proteomics

Diagnostics/Pathology

Immunology

Microbiology/Virology

BiochemistryGenomics/Genetics

Cell Biology

Molecular Biology

*Only those respondents who are either currently using or planning to use real-time PCR are included in this chart. n=567


10/12

10


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


Which real-time PCR thermocycler do you own?

ABI Prism® 7000MJ Research Chromo 4™

ABI Prism® 7900HTABI 7500Stratagene Mx3000P™Roche LightCycler® 2.0Bio-Rad MyiQRoche LightCycler®Cepheid Smart Cycler® TDIdaho Technology R.A.P.I.D™

Bio-Rad iCycler iQPyrosequencing Rotor-Gene 3000ABI 7300Stratagene Mx4000™Cepheid Smart Cycler® IIMJ Research DNA Engine Opticon®MJ Research DNA Engine Opticon® 2Other (please specify)

Do you use the real-time PCR reagents sold by your instrument’s

manufacturer or those sold by another company?I use kits and reagents sold by my instrument’s manufacturerI use kits and reagents sold by a company OTHER than my instrument’smanufacturerI use kits and reagents sold by BOTH my instrument’s manufacturer and a

company other than my instrument’s manufacturer

What does the company you purchase real-time PCR reagents from

offer that your instrument's manufacturer does not offer?

Lower pricesMy instrument’s manufacturer does not offer kits and reagentsWider selection of novel kitsFaster delivery

Better technical support/customer serviceBetter quality reagentsOther (please specify)

Do you currently perform or plan to perform real-time PCR?

Currently performPlan to perform

Neither currently perform nor plan to perform

What types of real-time assays do you perform?

5’ Exonuclease assay (ie. TaqMan probes)DNA Dye Binding Assay (ie. SYBER Green)Hybridization Assay (ie. Molecular Beacons, FRETprobes/primers)Other

Which of the following brands of real-time PCR

kits/reagents do you use?

Invitrogen UltraSense™BD Biosciences Clontech BD QZyme™MJ Research DyNAmo™Invitrogen ThermoScript™

Roche LightCyclerEpicentre MasterAmp™Epicentre FailSafe™QIAGEN® QuantiTect™Applied Biosystems TaqMan®Exiquon ProbeLibrary™Stratagene Brilliant™Other (please specify)

What format are you looking for when purchasing a PCR

master mix?

Master mix for use with hybridization probesMaster mix with SYBR GreenI make my own PCR master mixOther (please specify)

Do you own a real-time PCR thermocycler?YesNo


11/12

11


C o p y r i g

h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


What applications are you currently using or planning to use

real-time PCR for?

Primary validation of gene expressionPathogen detectionCancer diagnosticsMicroarray validationViral load titerSNP analysisOther

What type of template do you use?Genomic DNAcDNAPlasmid DNA

Are you performing or planning to perform HIGH

THROUGHPUT real-time PCR?

Yes, currently performing

Yes, planning to performNo

Do you own a real-time PCR workstation?

YesNo

Are you planning to purchase a real-time PCR workstation in

the next 3-6 months?YesNo

Do you plan to purchase a real-time PCR thermocycler in the

next 3-6 months?

YesNo

Which real-time PCR thermocycler would you consider

purchasing?

ABI Prism® 7000MJ Research Chromo 4™ABI Prism® 7900HT

ABI 7500Stratagene Mx3000P™Roche LightCycler® 2.0Bio-Rad MyiQRoche LightCycler®Cepheid Smart Cycler® TDIdaho Technology R.A.P.I.D™Bio-Rad iCycler iQ

Pyrosequencing Rotor-Gene 3000ABI 7300Stratagene Mx4000™Cepheid Smart Cycler® IIMJ Research DNA Engine Opticon®MJ Research DNA Engine Opticon® 2Other (please specify)

Which features are important to you when choosing a

thermocycler for real-time PCR?

MultiplexingSystem SpeedBroad-range of detectionHigh throughput abilityUniform temperature across the block/blocksOnline analysis capabilityRandom Access abilityIntuitive controlsOther (please specify)


12/12

12


C o p y r i g h t ©

2 0 0 4 B i o c o m p a r e ,

I n c . A


References

1. Mackay IM. Real-time PCR in the microbiology laboratory. Clinical Microbiology and Infection 2004; 10:190 – 212.

2. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996; 6: 986-94

3. Gwynne P and Heebner G. Drug discovery and biotechnology trends – Biochips 1: Microarrays on the move. www.science-

benchtop.org February 27, 2004.4. Gwynne P and Heebner G. PCR and cloning: A technology for the 21st century. www.science-benchtop.org February 9,

2001.

5. Invitrogen launches new tool for functional genomics. www.bioexchange.com July 31, 2002.
http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.bioexchange.com/http://www.bioexchange.com/http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.science-benchtop.org/http://www.science-benchtop.org/

realtimepcrexe

Documents