Institute of Industrial Science

SAKAI LAB.［Tissue Engineering for Regenerative Medicine

and Cell-Based Assay］Center for International Research on Integrative Biomedical Systems

http://envchem.iis.u-tokyo.ac.jp/sakai/index.php

Organs and Biosystems Engineering

Reconstruction and Utilization of TissuesToward applications to regenerative medicine for transplantation treatment andcell-based assays for drug and chemical screening, we have studied massproduction and differentiation control of progenitor stem cells, construction ofimplantable tissues, and development of cell-based assays.

TISSUE ENGINEERING

CIRIBiSMPUTC

Fe-505

Department ofChemical System EngineeringBioengineering

O2-

O2+

Monolayer

Multilayer

Cell sheet

Liver cell aggregates

Pancreatic islet cellaggregates

5 cm

Top viewSide view

Lung cells

Cell-Based Assays

Construction of 3D cell sheets under direct oxygenation

Development of the large artificial livercombined with 3D printing technology

Building blocks-based tissues for construction of large organs

Enhancement of liver metabolic capacityunder direct oxygenation

and application to drug screening testsConstruction of pancreatic islet models

for diabetes treatments

Development of lung cell-based assay systemsfor nanotoxicology

Biodegradable polymer

Immobilization of liver cells

Biodegradable polymer

Mass Production and Differentiation Control of Progenitor Stem Cells

Implantable Tissues

Mass production of iPS cells using microgel particles

Microgel particles (MGP)

iPS cells in MGP

Differentiation control of iPS cells to hepatocyte-like cells

iPS cells Hepatocyte-like cellsHepatocyte-like cells

O2- O2+

High metabolic capacityLow metabolic capacity

Side view Top view