red cell membrane
TRANSCRIPT
RED CELL MEMBRANE
CLINICAL LAB SCIENCES 3RD YR 5TH SEMESTER
CELL MEMBRANE OF RBC
2
RBC CELL MEMBRANE:
CONSIST OF:
PROTEIN 50% PHOSPHOLIPID 20% CHOLESTROL MOLECULES 20% CARBOHYDRATE 10%
3
THREE BASIC COMPONENTS OF RBC
CELL MEMBRANE
Lipid bilayer. Integral membrane protein. A membrane cytoskeleton.
4
1.LIPID BILAYER:
Consist of: Phospholipid. Cholestrol. Glycolipid. Integral protein. Peripheral protein.
5
2.MEMBRANE PROTEINS:
Peripheral protein:
Trophomyosin Spectrin Actin Protein 4.1 Ankyrin Protein 4.2
Integral protein:
Glycophorin Band 3 protein (chloride shift)
6
7
Names Definition Function
Peripheral protein
1. Spectrin
2. Actin
3. Ankyrin
4. Protein 4.1
5. Protein 4.2
6. Trophomyosin
cytoskeletal protein that lines the intracellular side of the plasma membrane Abundant protein in cell membrane
are a family of adaptor protein
is a major structural element.
is an ATP-binding protein
Heterodimeric protein
Responsible for biconcave shape of RBC
participates in more protein-protein interactions
Interacts with band 3 protein and spectrin to achieve linkage between bilayer andskeleton.
Stabilises actin-spectrin interactions.regulate the association of protein 3 with ankyrin.Stabilizing the actin filaments.
8
Names Definition Function
Integral protein
1. Glycophorin
2. Band 3 protein
Sialic acid rich glycoproteins
anion exchanger 1
imparts a negative charge to the cell,reducing interaction with other cells/endotheliumExchanges bicarbonate for chloride (chlorine shift).
9
3.CYTOSKELETON:
1. Formed by structural protein
2. Basic unit : hexagonal lattice with 6 spectrin molecules.
3. Tail end: tetramers linked to actin and protein 4.1. 4. Head end: ß spectrin linked to ankyrin
PLANE OF DESIGN:
1. Vertical interaction: • Stabilise the lipid bilayer membrane.
2. Horizontal interaction: • Maintain biconcave shape of RBC
10
11
12
DEFECTS IN RBC CELL MEMBRANE:
1.Hereditary spherocytosis:
• Hereditary hemolytic disorder. (most commom)• Mutation in membrane protein (spherical rbc)
• Clinical features: anemia. jaundice. fatigue. splenomegaly.
13
2. Elliptocytosis:
• Rare hereditary disorder.• also known as ovalocytosis.• Oval shape rbc.• It is a hemolytic anemia (severe cases)• Treatment: splenectomy
14
15
16