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Reference Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

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Page 1: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Reference Stream Sampling Status Report

Aquatic Ecology and Quality AssuranceMay 17, 2017

Page 2: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Background• Reference stream sampling began in 1992.• Sites were chosen to represent the least impaired

streams throughout Florida.• Sites had relatively small watersheds wholly

contained within a given subecoregion.• Eighty-five sites were on the original reference list.

District Reference Sites

NW 31

NE 20

SW 15

CD 12

SO 4

SE 35/2017 Biocriteria/ROC Fest 2

Page 3: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Location of Original Sites

5/2017 Biocriteria/ROC Fest 3

Page 4: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

• Re-evaluate historic reference streams.• Establish a revised list of reference streams.• Document changes in the conditions over time.

5/2017 Biocriteria/ROC Fest 4

Objectives

Page 5: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

List of Analytes

• Stream Condition Index • Calcium • Rapid Periphyton Survey • Sulfate

o Algal ID sample, if periphyton is abundant. • Linear Vegetation Survey • Chloride • Habitat Assessment • Sodium • Hydrologic Modification Characterization (per SOP FT3101) • Potassium • Chlorophyll-a • Carbonate • Turbidity • Magnesium • Alkalinity • Bromide • Color • Sucralose • Ammonia • Acetaminophen • Nitrate+nitrite • Total Organic Carbon • Total Phosphorus • Total Dissolved Solids • Total Kjeldahl Nitrogen • qPCR – HF183

• Total Suspended Solids • Fecal coliforms & e. coli

5/2017 Biocriteria/ROC Fest 5

Page 6: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

LIMS and SBIO Project• The following data were retrieved from LIMS under

“SCIREF” and “EcoRegional Reference Stream” in SBIO. • If samples were scheduled/entered under another

project (e.g. SMP) they are not included.• In the future, if reference stream samples are collected

for multiple purposes, use the projects listed above. SMP lists are generated from the IWR database and include all standard projects.

5/2017 Biocriteria/ROC Fest 6

Page 7: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

SCI Summary by Sampling Group2015 – 2016

Graphs include only samples marked complete as of 4-27-17.

1210

2

7

18

7

11

53

10

7

CEN NE NW SE SO SW TLH

SCIs Completed by Sampling Group

2015 2016

73 7770 75

63 6760

AEQA CEN NE NW SE SO SW

Average SCI Score

5/2017 Biocriteria/ROC Fest 7

Page 8: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

SCI & SCI Metrics by BioRegionBig Bend Northeast Panhandle Peninsula

Average 79 64 72 66Minimum 64 37 52 19Maximum 92 81 88 92St. Dev 9.9 13.2 10.6 15.2

2 3

86

9

32

3

10

21

29

2 36

47

29

4

9

2427

4 4

107

13

35

1

7

1922

1 36

4 4

27

1012

27 26

0

5

10

15

20

25

30

35

40

SCI Metrics - BioRegion

Big Bend 2012 Northeast 2012 Panhandle 2012 Peninsula 2012

5/2017 Biocriteria/ROC Fest 8

Page 9: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Big Bend – SCI Results

83

73

64

87 88

75

91

69

85

67

71

78

0

10

20

30

40

50

60

70

80

90

100

Aver

age

SCI

Sco

re

McBride Slough - Sept 3, 2015

Steinhatchee River – May 2, 20175/2017 Biocriteria/ROC Fest 9

Page 10: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

NE – SCI Results

63

79 79

58

7269

63 60

41

0

10

20

30

40

50

60

70

80

90

Aver

age

SCI S

core

*STJOHNDEP – Deep Bottom Creek

5/2017 Biocriteria/ROC Fest 10

Page 11: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Panhandle – SCI Results

73

56

79

65

86 8784

73

53

68 67

82

62

80

84

61 61

70

63

81 8178

0

10

20

30

40

50

60

70

80

90

100

Aver

age

SCI S

core

Sopchoppy River - September 3, 2015

Econfina Creek – February 9, 20175/2017 Biocriteria/ROC Fest 11

Page 12: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Peninsula – SCI Results

63

72

63

42

68

87

7376

62

55 56

84

78

63

70

57

66

71

59

69

85

19

0

10

20

30

40

50

60

70

80

90

100

Aver

age

SCI S

core

s

Hillsborough River - April 21, 2015(photo provided by J. Ashton)

Rainbow River - December 4, 2016(photo provided by M. Linger)

5/2017 Biocriteria/ROC Fest 12

Page 13: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

SCI Score Percentile Ranks

0 10 20 30 40 50 60 70 80 90 100

WILLMSREF

LTCRKCKREF

NWFRLOXREF

SMRSO23C

MCSTLMLCR

BAKER8

MOSQTUNK

COFFEEREF

ROCKYWDSRD

MCBRIREF

MOULTRCRK

STEINCANAL

BETHEL@MIL

HILSTP5REF

THOMMOLR…

BLKCKNPREF

NATBRDGREF

MCDAVREF

LVNGSTNREF

TIGERREF

ECONSCTREF

LLOYDREF

1941

4253

5656

5758

59606161

6262636363636363

646566

6767

6868

696969

7070

7171727273737373

7576

78787879797980

8181

8283

848484

8585

8687878788

91

Aver

age

SCI S

core

Drop from ref list?

5/2017 Biocriteria/ROC Fest 13

Page 14: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Nutrients – Descriptive Statistics

Analyte N* Mean Std Dev Median Min Median 75%

CHL-A 62 1.592 1.176 1.14 0.69 1.14 1.696

NO2NO3 62 0.268 0.376 0.049 0.004 0.13 0.344

TKN 62 0.655 0.589 0.0761 0.125 0.492 0.845

TN 62 0.888 0.641 0.0814 0.181 0.79 1.06

TP 62 0.152 0.223 0.0284 0.005 0.0715 0.18

*Note: Twenty-two (22) samples were removed for fatal qualifier codes.

5/2017 Biocriteria/ROC Fest 14

Page 15: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Average Chlorophyll-a

3.2

5.7

3.7

6.06.5

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

CHLO

ROPH

YLL-

A (U

G/L

)

AXIS TITLE

Five sites had elevated chlorophyll-a levels: Smith, Mosquito, South Fork Black, Gap, and Livingston Creeks

5/2017 Biocriteria/ROC Fest 15

Page 16: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

TOTAL NITROGENNOx + TKN

-0.30

0.03

0.36

0.69

1.02

1.35

1.68

2.01

2.34

2.67

3.00

1.602.00

0.79 0.671.20

0.78

NO2NO3 TKN

TKN accounted for a large fraction of TN at most sites. Notable exceptions were the Chipola, Flat, Lloyd, Mayo Mill, McBride, Rainbow, Cedar Run, Choctawhatchee, Hillsborough, Little Manatee, and Manatee Rivers where NOx made up half or more.

5/2017 Biocriteria/ROC Fest 16

Page 17: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

NO2+NO3

1.60

0.370.48

0.35

2.00

0.790.67

0.37 0.39

1.20

0.69

0.50

0.78

0.00

0.33

0.66

0.99

1.32

1.65

1.98

2.31

AUCI

LLA

BETH

ELBR

IDGE

CHIP

ORE

FCR

OO

KED

ECO

NFI

NA

CREE

KEC

ON

FIN

A RI

VER

FLAT

LITT

LE C

ROO

KED

LLO

YDM

AYO

MIL

LM

CBRI

DERO

CKY

SAN

DYSF

BEA

RST

EIN

RIV

ERSW

EETW

ATER

TEN

MIL

EW

ELRE

FBE

ARDA

VEN

PORT

MO

RMO

NRA

INBO

WTI

GER

ATES

CEDA

R RU

NDE

EP B

OTT

OM

FALL

ING

GOLD

HEA

DLI

TTLE

MCG

IRTS

MO

ULT

RIE

PETE

RSRO

BIN

SON

S. P

RON

G ST

MAR

YSSI

X M

ILE

CHO

CTAW

RIV

EREI

GHT

MIL

EN

ATU

RAL

BRID

GEFO

RT D

RUM

MO

SQU

ITO

NW

FRLO

XREF

TIG

ERHO

RSE

ORA

NG

EPO

PASH

POW

ELL

BLAC

K SF

FISH

HAW

KFR

YEU

NK

GAP

HILL

SREF

HORS

ELI

TTLE

MAN

ATEE

LIVI

NG

STO

NM

ANAT

EEO

AKRE

FPI

TH

NIT

RATE

+NIT

RITE

(MG

/L)

Thirteen sites had ≥ 0.35 mg/L nitrate: Chipola, Flat, Lloyd, Mayo Mill, McBride, Rainbow, Cedar Run, Choctawhatchee, Mosquito, Horse, Hillsborough, Livingston, and Little Manatee, Livingston, and Manatee

5/2017 Biocriteria/ROC Fest 17

Page 18: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

TOTAL PHOSPHORUS

0.510

1.500

0.000

0.200

0.400

0.600

0.800

1.000

1.200

1.400

1.600

TOTA

L PH

OSP

HORU

S (U

G/L

)

AXIS TITLE

Williams Creek was an order of magnitude higher than most sites.Williams is also the site that had a 19 and 37 for SCI scores. We suggest removing it from the pool of reference sites.

5/2017 Biocriteria/ROC Fest 18

Page 19: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Bacteria

816887

1553

0

200

400

600

800

1000

1200

1400

1600

1800

FLAT SWEETWATER CROOKED

MPN

/100

mL

E. Coli-QT(samples that exceed TPTV ≤410 CFU/100mL)

52 82 104 144 200 200 250 300 410 480 520817

4800

0

1000

2000

3000

4000

5000

6000

CFU

/100

mL

F. Coli-MF

5/2017 Biocriteria/ROC Fest 19

Page 20: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

Bacterial IndicatorsVery few acetaminophen samples were collected. Check scheduling templates to make sure it is included.

1.7

7.2

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

LIVI

NG

STO

NM

CBRI

DEFI

SH H

AWK

MO

ULT

RIE

NW

FRLO

XREF

CHIP

OLA

MO

SQU

ITO

BRID

GEDE

EP B

OTT

OM

POPA

SHSI

X M

ILE

GAP

MCG

RITS

PITH

POW

ELL

BEAR

CHO

CTAW

SAN

DYO

RAN

GE

BLAC

K SF

ORK

FRYE

DAVE

NPO

RTPE

ACE

ug/L

Sucralose

1 1 6.8 15 22 43

650

1610

0200400600800

10001200140016001800

GEU

/100

mL

HF183-qPCR

5/2017 Biocriteria/ROC Fest 20

Page 21: Reference Stream Sampling Status Report - Florida Dep Stream Sampling Status Report Aquatic Ecology and Quality Assurance May 17, 2017

5/2017 21