report of laboratory exercises
TRANSCRIPT
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Report of laboratory
exercises
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Application of flame atomic absorption
spectrometric techniques to pharmaceuticals
and waters
Atomic absorption specroscopy is analytical method characterized with high
selectivity,sensitivity and fast speed.
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Three different brands of multivitamins (effervescent tablets) were taken from the market
and the levels of some elements (total amount) were determined (Ca, Mg, Zn, Cr, Mn)
One tablet from each of the three pharmaceutical products was dissolved in 100ml (mother
solution) water and one milliliter of each solution was diluted 25 times. So were prepared the
working sample solutions and were marked as follows:
Sample 1 (samp1) - ABO Pharma A-Z ;
Sample 2 (samp2) - Supradyn ;
Sample 3 (samp3) - Supravit Active ;
A brand of mineral water - Gorna Banja was tested for any Mg and the concentration of
the metal was under the detection limit of the instrument
For each different element was made a standard calibration curve, using freshly prepared
standard solutions from 0,5; 1; 3; 5; 10; 20ppm containing all the mentioned elements in water
(deionized)
For the last sample results were obtained both after automated calibration and a manually
made calibration curve.
Differences in the absorption using reducing and oxidizing flames were observed
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2.110%3
2.410%2
1.910%1
Measuredvalues
[ug/tablet]
# Sample
Pb calibration curve (ET AAS)
y = 3,775x + 8,3333
R2
= 0,9909
0
50
100
150
200
250
300
350
0 20 40 60 80 100
concentration [ppb]
absorption
=283.3 nm
1.910%3
1.610%2
8.910%1
Measured
values
[ug/tablet]
# Sample
Cd calibration curve (ETAAS)
y = 144,38x + 8,8333
R2
= 0,9828
0
50
100
150200
250
300
0 0,5 1 1,5 2
concentration [ppb]
absoptio
n
=228.8 nm
E t AA S
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Sample 1 (samp1) - ABO Pharma A-Z ;
Manufacturer: ABO Pharma AG
Sample 2 (samp2) - Supradyn ;
Manufacturer: Bayer AG
Sample 3 (samp3) - Supravit Active ;
Manufacturer: Kendy Ltd Bulgaria
Patented by Swisse AG
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IR spectra of benzocaine
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CH3, CH2
def. vibr.
CH3 (end)def. vibr.
C-H str. vibr
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KBr Spectra benzocaine
-NH2
-CH2
-C=O-NH2
-Ar
C-C
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Thank You for Your
attentionLora Georgieva
Denitsa AtanasovaKrum Bardarov