reproductive cloning biotechnology/cloning. reproductive cloning

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Reproductive cloning Biotechnology/Cloning

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Page 1: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Reproductive cloning

Biotechnology/Cloning

Page 2: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Reproductive cloning

Page 3: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Therapeutic cloning

Page 4: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

http://www.rndsystems.com/dam_public/6515.jpg

Therapeutic cloning

Page 5: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Recombinant DNA technologyDNA isolated from

two sources and cut by the same enzyme

Gene of interest

RecombinantDNA

Plasmid(vector)

Transgenic organisms

Useful products

Gene for pestresistance

Gene fortoxic-cleanup

bacteria

Some uses of proteins:

Protein for“stone-washing”

jeans

Protein fordissolving

clots

Some uses of genes:

Page 6: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Restriction Enzymes

Page 7: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

First Cloning Experiment

Cohen et al., 1973

“Recombinant DNA”

Page 8: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Cloning Vectors: pBR322 plasmid

Cloning into pBR322

Page 9: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

RT-PCR and Cloning

Page 10: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615

“Supermice”

Page 11: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Golden Rice

Page 12: Reproductive cloning Biotechnology/Cloning. Reproductive cloning

Weeding Out Land MinesFirst Glowing Pet