research strategy for human organ fabrication-pig as in vivo ...organfabri.med.keio.ac.jp/aachen...
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Research Strategy for Human Organ fabrication-Pig as In vivo Bioreactor-
Eiji Kobayashi MD PhDDepartment of Organ Fabrication Keio University School of Medicine Japan
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Surv
ival
Rat
e (
)
0 1 2 3 4 5 6 Year
From 2001 to 2009年n=140
(Transplant Proc 2010)
(J Hepatobriary Panc Sci 2013) (Transplant Proc 2009)
Declaration of Istanble(Istanbul 30th April ndash 3rd May 2008)
152 professionals from 78 countries（Ｌａｎｃｅｔ ２００８）
Human BodyldquoProductionrdquoーThe impact of Regenerative Medicineー
ＮＨＫ Special ２００９
Eiji Kobayashi MDJichi Medical university
下村 修先生
Green Fluorescence ProteinOsamu Shimonura
Novel Prize in Chemistry ２００８
Lung 25 min after LPS injection
Granulocyte９８２
Lymphocyte７７０
FSC
(Sakuma Y et alTransplant Immunol 2004)
Benefits in bio-imaging system using
(Sato A et al Anesth Analg 2005)
lsquofluorescencersquo
Transplanted lung immediately after re-perfusion
(Enomoto A et al Microsurgery 2007)
Aequoreavictoria
Migration
Differentiation from the fetal tissue
Cerebral infarction
Red Nestin Red GFAP
(Inoue H et al BBRC 3292882005)
Neurosphere
The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nervesBozkurt A Lassner F ODey D Deumens R Boumlcker A Schwendt T Janzen C Suschek CV Tolba R Kobayashi E Sellhaus B Tholl S Eummelen L Schuumlgner F Damink LO Weis J Brook GA Pallua NBiomaterials 2012 Feb33(5)1363-75 doi 101016jbiomaterials201110069 Epub 2011 Nov 13
The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nervesBozkurt A Lassner F ODey D Deumens R Boumlcker A Schwendt T Janzen C Suschek CV Tolba R Kobayashi E Sellhaus B Tholl S Eummelen L Schuumlgner F Damink LO Weis J Brook GA Pallua NBiomaterials 2012 Feb33(5)1363-75 doi 101016jbiomaterials201110069 Epub 2011 Nov 13
Promising future for the transgenic rat in transplantation researchDoorschodt BM Teubner A Kobayashi E Tolba RHTransplant Rev (Orlando) 2014 Oct28(4)155-62 doi 101016jtrre201405002 Epub 2014 May 27 Review
NCI
International Collaboration using Photonics Rats
Singapore
RWJ Med Sch
AustraliaGA Bishop (Sydney)
USAJohn Critser (Missouri Univ)SS Thorgeirsson (NCI)P Leone(RWJ Med Sch)Germany
EK Geissler(Regensburg)G Nikkhah (Freiburg)R Tolba (Bonn)
SingaporeDW HutmacherS Cool (Bioporis)
Missouri Univ
Toronto Univ
CanadaA Keating (Toronto Univ)
Freiburg UnivRegensburg Univ
Bonn Univ
Experimental Microsurgery
1992 1st ISEM Rome Italy
1994 2nd ISEM Kanazawa Japan
1996 3rd ISEM Wuerzburg Germany
1998 4th ISEM LondonOntario Canada
2000 5th ISEM Catania Italy
2002 6th ISEM San Diego USA
2004 7th ISEM Debrecen Hungary
2006 8th ISEM Montreal Canada
2008 9th ISEM Shanghai Peoplersquos Republic of China
2010 10th ISEM Sao Paulo Brazil
2012 11th ISEM Timisoara Romania
2014 12th ISEM Kyoto Japan
2016 13th ISEM Tianjin China
2018 14th ISEM Debrecen Hungary
Clinical Microsurgery1986 A-V shunt operation
2000 Hepatic artery reconstructionin liver transplantation 2002 6th ISEM San Diego USA
2004 7th ISEM Debrecen Hungary
2008 9th ISEM Shanghai Peoplersquos Republic of China
2010 10th ISEM Sao Paulo Brazil
Defining Standards in Experimental Microsurgical Training Recommendations of the European Society for Surgical Research (ESSR) and the International Society for Experimental Microsurgery (ISEM)
Tolba RH Czigaacuteny Z Osorio Lujan S Oltean M Axelsson M Akelina Y Di Cataldo A Miko I Furka I Dahmen U Kobayashi E Ionac M Nemeth NEur Surg Res 201758(5-6)246-262 doi 101159000479005 Epub 2017 Jul 26
Present Future
11x109 28x109 01x105
Hemizygote Homozygote WT littermate
ROSA 26 Luciferase
Photnus pyralis Firefly Tg Rat
(Hakamata Y et al Transplantation 2006)
LuminescenceLuciferin
Luciferase+
Materials and Methods
蛍ラット
Light Emission of Luciferase Transgenic Ｒat
(Hakamata Y et al Transplantation 2006)
POD 1 3 7 14 3521 63
Rel
ativ
e ex
pres
sion
inde
x
Days after hepatocytes transplantation
0
100
200
300
400
500
0 7 14 21 28 35 42 49 56 63 70
Hepatectomy
Normal
Chase the transplanted hepatocytes
Normal
Hepatectomized
(Hakamata Y et al Transplantation 2006)