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    School-based assessment

    Practical related tasks

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    Osmosis in Living Tissue

    To determine the water potential of potatotissue.

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    Curriculum Link

    C1 Water for Living

    Movement of water in and out of cells by osmosis

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    Background

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    How can we determine the water potential of

    potato tissues?

    Potato

    1.0 M Sucrose solution

    (= 342 g sucrose in 1 L of solution)

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    Apparatus and Chemicals

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    Procedure (1)

    A series of sucrose solution of

    different concentration.

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    Procedure (2)

    Cut potato cylinders into length of 5 cm.

    Blot dry the potato cylinders and put them into a Petri dish.

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    Procedure (3)

    Immerse 2 potato cylinders in

    each tube that contains different

    concentration of sucrose

    solution.

    After 40 mins, take out the

    potato cylinders, blot dry and

    measure the length of the potato

    cylinders.

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    Result Table

    1. Tabulated result :

    Notice the position of independent variable,dependent variable in the table.

    Units and suitable conversions should be shown

    clearly and correctly

    Title of the table should be written correctly according

    to the aim of the experiment.e.g. Effect of sucrose concentration on thee.g. Effect of sucrose concentration on the

    length of potato cylinderlength of potato cylinder.

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    Effect of sucrose concentration on the percentage

    change in length of potato cylinder

    - 3.04.854.84.950.6

    - 1.04.954.95.050.4

    - 5.04.754.74.850.8

    4.6

    5.1

    5.2

    - 7.04.654.751.0

    + 3.05.155.250.2

    + 5.05.255.350

    % change in% change in

    length of potatolength of potatocylinderscylinders

    AverageAverage

    ffinal lengthinal length

    of potatoof potatocylinderscylinders

    (cm)(cm)

    FFinal lengthinal length

    of potatoof potato

    cylinderscylinders

    (cm)(cm)

    OOriginalriginal

    length oflength of

    potatopotatocylinderscylinders

    (cm)(cm)

    concentrationconcentration

    of sucroseof sucrosesolutionnsolutionn/ M)/ M)

    Key : + increase in lengthKey : + increase in length

    -- decrease in lengthdecrease in length

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    Graph

    Notice the choice of axes

    x-axisfor independent variable (unit should be stated)

    y-axisfor dependent variable (unit should be stated)

    Notice the rule of using short straight lines to join

    the points or the smooth curve to join the points Title of graph should be added correctly and related

    to the aim of the experiment

    e.g. Effect of sucrose solution on the % change in length of potato

    cylinders

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    Effect on sucrose concentration on the %Effect on sucrose concentration on the %Effect on sucrose concentration on the %Effect on sucrose concentration on the %

    change in length of potato cylinderchange in length of potato cylinderchange in length of potato cylinderchange in length of potato cylinder

    -8.0-8.0-8.0-8.0

    -6.0-6.0-6.0-6.0

    -4.0-4.0-4.0-4.0

    -2.0-2.0-2.0-2.0

    0 .00.00.00.0

    2 .02.02.02.0

    4 .04.04.04.0

    6 .06.06.06.0

    0000 0.20.20.20.2 0.40.40.40.4 0.60 .60.60 .6 0.80.80.80.8 1111 1.21.21.21.2

    Concentration of sucrose solution / MConcentration of sucrose solution / MConcentration of sucrose solution / MConcentration of sucrose solution / M

    %c

    hangeinleng

    thofpotato

    %c

    hangeinleng

    thofpotato

    %c

    hangeinleng

    thofpotato

    %c

    hangeinleng

    thofpotato

    cylinders

    cylinders

    cylinders

    cylinders

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    Laboratory Report Title

    Aims

    Theory/Background (optional)

    Apparatus

    Design of experiment

    Procedure

    Precautions and safety measures

    Results (including data and observations)

    Discussions (including possible sources of errors andimprovements to be made)

    Conclusion

    Questions for further thought (optional)

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    Assessment CriteriaArea A (Max:10)

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    Assessment criteriaArea B (Max:10)

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    Assessment CriteriaArea B (Contd)