Results of the implementation Results of the implementation of the coof the co--financed Rabies financed Rabies eradication programme eradication programme

in Hungary in 201in Hungary in 20111

National Food Chain Safety OfficeDirectorate of Animal Health and Animal Welfare,

Hungary

Sept 2012 SCoFCAH, Brussels

Oral immunisation programmeOral immunisation programme− Target species: Red fox (Vulpes vulpes)‏

− 2 vaccination campaigns: Spring campaign : 2-17 April 2011Autumn campaign: 1-16 October 2011

− Vaccinated area: Specified part of the country (2x)‏planned: 2 x 46 326 km2 = 92 652 km2

− Vaccine type: Lysvulpen

− Quantity: planned: 1 800 000 doses

SCoFCAH, Brussels

Oral immunisation programme IIOral immunisation programme IIDistribution of the vaccine baits: Distribution of the vaccine baits:

~98% by airplane:− GPS system, flying lines recorded by computer,− Distance between flying lines: 1000 m (in general)− Flying speed: 100-120 km/h,− Density: 20 baits / km2 (gross)‏− Flying lines rotated with 90° in the next campaign.

~2% manually:− Where flying not allowed or− More targeted distribution needed,− Carried out by qualified wild life biologists.

SCoFCAH, Brussels

Areas vaccinated in 2011 (spring Areas vaccinated in 2011 (spring and autumn) and autumn) –– flying lines are flying lines are

showed with red colourshowed with red colour

Control of vaccinationControl of vaccinationNumber of foxes to be tested in 20112011 4575 foxes

SCoFCAH, Brussels

Implementation of sampling:− Hunting period: 30 - 100 days after vacc. camp.Official hunters:

organized hunting + collection of dead foxes

Control of vaccination Control of vaccination

SCoFCAH, Brussels

Laboratory background:

Veterinary Diagnostic Directorate of the National Food Safety Office:

• Budapest (National Reference Laboratory)

• Debrecen (regional laboratory)

• Kaposvár (regional laboratory)

Control of vaccination Control of vaccination

SCoFCAH, Brussels

Monitoring on efficiency of the oral immunization

― direct immunfluorescence of brain imprints

― biomarker tetracycline test (bone polishing of the mandible) – test for bait uptake (only in NRL Budapest),

― serological test (ELISA) – (only in NRL Budapest),

― RFFIT (Rapid Fluorescent Focus Inhibition Test) determination of the titre of antibodies of the rabies virus in the blood serum (a clot from the heart or liquid from the chest) – test for immunisation,

― collection, handling and analysis of epidemiological data about diagnosed cases.

Control of vaccinationControl of vaccination

SCoFCAH, Brussels

Laboratory results in 2011 (in red foxes):

Virological tests: Samples tested Positive Samples tested Positive 4575 4575 0 0

Serological tests: Samples tested Positive Samples tested Positive

553 553 173173

Bone polishing: Samples tested Positive Samples tested Positive

2201 2201 14321432

8.

Rabies cases in HungaryRabies cases in Hungary

SCoFCAH, Brussels

In 2010 : 11 positive cases: 9 foxes, 1 dog, 1 bat

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Rabies cases in HungaryRabies cases in HungaryIn 201In 20111 -- 22 batsbats

Location of rabies cases in Hungary (201Location of rabies cases in Hungary (20111))batbat casescases

SCoFCAH, Brussels 10.

Financial contribution Financial contribution (without VAT)(without VAT)

SCoFCAH, Brussels

The amount spent on oral immunisation programme carried out in red foxes in Hungary in 2011 contains the following :

- Costs of the vaccination 1 716 872 EUR- Cost of sampling 101101 576576 EUR- Costs of the testing 9797 878722 EUR- Costs of public procurement 1 780 EURTotal costs: 1 918 100 EUR

Community co-financing (Commission Decision 2010/712/EC)

1 450 000 EUR

Thank you for your attention!Thank you for your attention!