revolutionary streamlined and rapid n …...based glycan purification in a fully automated sample...

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EZGlyco™ mAb-N Kit with 2-AB

REVOLUTIONARY STREAMLINED AND RAPID N-GLYCAN PREPARATION DIRECTLY FROM IgG IN CELL CULTUREThe Kit circumvents multiple sample transfers and drying steps and bypasses a separate IgG purification step, making the operation fast and simplified.

BRINGING GLYCOMICS TO LIFE®

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EZGlyco™ delivers results in less than two hours, allowing you to purify IgG from cell culture supernatant and release and label N-glycan in one complete kit.

• HPLC-ready glycan preparation in less than 2 hours

• Purify IgG and Fc-fusion protein from cell culture supernatant

• Release and label N-glycans

• Clean-up and ready to analyze

EZGlyco™ mAb-N Kit with 2-AB is a streamlined and rapid N-glycan preparation kit for IgG from cell culture supernatant with unmatched speed, ease of use, and compatibility with HPLC.

• Capturing of IgG from cell culture supernatant

• Release of glycans

• Labeling of glycans

• Purification of labeled glycans

WHEN TIME MATTERS, CHOOSE EZGLYCO™. HPLC-Ready in Under 2 Hours

RELIABILITY AND ACCURACY DELIVERED. Streamlined N-glycan Sample Preparation

Capturing of IgG

Release of glycans Labeling of glycans

Purification of labeled glycans

HPLC-ready sample preparation from cell supernatant

in less than 2 hours.

mAb in cell culture supernatant

Application Data• N-glycan profiling of human IgG using the EZGlyco™ mAb-N Kit with 2-AB. • 10 μg hIgG was spiked in culture medium.• N-glycans were prepared and labeled with 2-AB according to the instructions.• An aliquot of 0.2 μg IgG was analyzed using HPLC.

The Kit circumvents multiple sample transfers and drying steps and bypasses a separate IgG purification step, making the operation fast and simplified.

RETENTION TIME (MIN)

N-GLYCAN PREP MADE EASY WITH A SINGLE TUBE OPERATION

EZGlyco™ mAb-N Kit with 2-AB


WHAT YOU GET WITH EZGLYCO™. ContentsAntibody Capturing Column (Qty 10)Silicone Bottom Cap (Qty 10)Antibody Capturing Solution (Qty 1)Washing Buffer (Qty 1)Glycan Release Enhancer (Qty 1)PNGase F Solution (Qty 1)2-Aminobenzamide (Qty 3)Reducing Reagent (Qty 3)Cleanup Column (Qty 10)Instruction (Qty 1)

The EZGlyco™ mAb-N Kit with 2-AB is an innovative product that streamlines N-glycan sample preparation from IgG in cell culture media, through accelerated deglycosylation and fluorescent labeling reactions in a single column.

EASY TO USE AND INNOVATIVE. Simplified Single-Tube Operation

The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant.

LEADING-EDGE COMPATIBILITY. HPLC-Ready N-glycan Preparation

Order EZGlyco™ at s-bio.com/ezglyco-mab-nORDER EZGLYCO™ TODAY.

Watch the EZGlyco process at

s-bio.com/ezglyco-mab-n

S-BIO is a division of Sumitomo Bakelite Co., Ltd. (a pioneer in plastics with products used for a wide-ranging applications such as IT components and material, high performance plastics and quality-of-life product). S-BIO in US operates as a part of Vaupell Holdings Inc., a group company of Sumitomo Bakelite Co., Ltd. S-BIO’s main product and services fall into two categories: glycan analysis related services and products, and high performance labware for cell culture applications.


SERVICESS-BIO specializes in the rapid and efficient analysis of both N-linked and O-linked glycans present on antibodies and other glycoproteins. Our proprietary GlycanMap® and GlycanMap Xpress® Services that combines BlotGlyco® bead-based glycan purification in a fully automated sample processing system with high-throughput MALDI-TOF mass spectrometry to generate rapid, reproducible glycan profiles. The resulting qualitative and quantitative information can be employed to achieve a variety of endpoints related to strain selection, fermentation and process development, formulation stability and product characterization. S-BIO’s services provide a new solution to accelerate your biotherapeutic development and biomarker discovery programs by enabling accelerated glycoform analysis across literally hundreds of samples.

GLYCAN ANALYSIS PRODUCTSWe recognize that many organizations prefer to perform analysis in-house for a variety of reasons. S-BIO offers its technology to customers in the form of BlotGlyco® beads and purification and labeling kits especially designed for glycan analysis. S-BIO also offers printed Glycan Array and Plates for binding assay.

GLYCAN ANALYSIS PRODUCTS AND SERVICES.S-BIO offers high-throughput analysis services and kits based our BlotGlyco® polymer beads for supplementary glycan purification.

revolutionary streamlined and rapid n …...based glycan purification in a fully automated sample...

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