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Ribozymes (RNA Enzymes) David Peterson

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Page 1: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Ribozymes(RNA Enzymes)

David Peterson

Page 2: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Enzymes•Catalyze reactions

•Very specific

•Act on substrates

•Active site

Page 3: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Enzymes Bind Substrates

Page 4: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Enzymes Bind Substrates

Page 5: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Enzymes Bind Substrates

Page 6: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Structure of RNA and DNA

Page 7: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site
Page 8: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

DNA RNA Protein

Information Flow in CellsThe Central Dogma of Molecular Biology

Transcription Translation

Page 9: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Semiconservative DNA Replication

Page 10: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site
Page 11: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Amino acids combine to make peptides and proteins

Page 12: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

mRNA Splicing

Page 13: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Ribosomes

• Site of protein synthesis

• Made of RNA and protein

Page 14: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Tetrahymena thermophila

Page 15: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Self-Splicing RNA

Cech, Zaug, and Grabowski.1981. Cell 27: 487-496

Page 16: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Single-Stranded RNA Can Form Intramolecular Base Pairs

Page 17: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Intramolecular H-bonds in the Tetrahymena Ribozyme

Page 18: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

3D Structure of the Self-Splicing Intron

Page 19: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Ribosomes

• Site of protein synthesis

• Made of RNA and protein

Page 20: Ribozymes (RNA Enzymes) David Peterson. Enzymes Catalyze reactions Very specific Act on substrates Active site

Tom Cech Sidney Altman

Nobel Prize in Chemistry 1989