rr322304 bioprocess engineering ii

Code No: RR322304 Set No. 1

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006BIOPROCESS ENGINEERING-II

(Bio-Technology)Time: 3 hours Max Marks: 80

Answer any FIVE QuestionsAll Questions carry equal marks

1. How can you classify the bioreactors based on the operational mode and explainabout them ? [16]

2. Write short notes on: [8+8]

(a) One-parameter model

(b) Two-parameter model

3. Explain about Stability analysis of bioreactors? [16]

4. How can you determine [6+4+6]

(a) Cellular ATP content

(b) Single cell characteristics

(c) Assay of cells with and without plasmids.

5. Discuss in detail about computer interfaces and peripheral devices. [16]

6. Explain in detail about the various stages in gene expression. [16]

7. Explain the integration of major metabolic pathways in an animal cell. [16]

8. Discuss in detail about growth cycle phases for batch cultivation. [16]

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1. What are the different types of the bioreactors employed in Animal Cell Culture ?Explain about packed glass bead reactors and Perfusion cultures ? [16]

2. Write short notes on: [6+4+6]

(a) Filter sterilization of liquids

(b) Continuous sterilization

(c) Sterilization of gases.

3. Name the type of bioreactor in which heterogeneous biocatalyst particles are sus-pended by drag forces exerted by rising liquid and explain in detail about it witha neat diagram? [16]

4. Outline the procedures involved in the quantification of total RNA content andtotal Protein content in microbial cells ? [16]

5. Discuss in detail about gas analysis system based on a microcomputer with schematicdiagram. [16]

6. Explain in detail about the various stages in gene expression. [16]

7. Discuss in detail about product formation kinetics. [16]


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1. Explain the basis for classification of Bioreactors and write in brief about themwith an example? [16]

2. Write short notes on: [8+8]

(a) Spiral heat exchanger

(b) Flash cooler sterilizer.

3. Explain the design and operation of Fed-batch reactor with mass-balances? [16]

4. Write in detail about the methods employed in measuring the following processvariables. [8+8]

(a) Temperature

(b) Pressure.

5. Explain the design and operating strategies for batch plants. [16]

6. Explain the guide lines for choosing Host-Vector system. [16]

7. Discuss in detail the scale-up of animal cell cultivation using BHK. [16]


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1. List out the different accessories of a bioreactor and their uses ? [16]

2. Write short notes on: [16]

(a) Non-Ideal Tubular reactor

(b) Non-Ideal CSTR.

3. Explain about Stability analysis of bioreactors? [16]

4. What is Flow injection instrumentational analysis? Explain its role in bioprocess-ing? [16]

5. (a) Discuss in detail about ON-Line Sensor.

(b) Discuss in detail about OFF-Line Analytical Methods [8+8]

6. Explain in brief different types of products of Genetic Engineering. [16]

7. Write Short Notes On: [16]

(a) Monoclonal antibodies.

(b) Immunobiological Regulators.

(c) Virus Vaccines.

(d) Hormones.


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rr322304 bioprocess engineering ii

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