RT-PCR: PRKWNK1,WNK1, T39 T40 (232 bp),Reamplifikation

040402_Reampli_Gewebe_1_A1_T39T40_calb

Bedingungen:2% TBE90V 1h 40min9 µ Reampli-Probe2 µl LB

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24M

M 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M

M = Marker1 = Oesophagus2 = Stomach3 = Stomach Tumor4 = Corpus5 = Antrum6 = Small Intestine7 = Duodenum8 = Large Intestine9 = Large Intestine10 = Coecum11 = Coecum12 = Colon13 = Colon Tumor14 = Rectum15 = Rectum16 = Cervix17 = Endometrium18 = Uterus19 = Uterus Tumor20 = Ovary21 = Ovary Tumor22 = Ovary Tumor23 = Placenta24 = Testis

25 = Testis Tumor26 = Prostate gland27 = Prostate gland28 = Adrenal gland29 = Adrenal gland30 = Kidney31 = Kidney Fetal32 = Kidney Tumor33 = Kidney Tumor34 = Bladder35 = Spleen36 = Spleen37 = Spleen Fetal38 = Liver39 = Liver40 = Liver Fetal41 = Pancreas42 = Pancreas43 = Salivary gland

RT-PCR: PRKWNK1,WNK1, T39 T40 (232 bp),Reamplifikation

Bedingungen:2% TBE90V 1h 40min9 µl Reampli-Probe2 µl LB

040402_Reampli_Gewebe_2_A1_T39T40_calb

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M

M = Marker1 = Thyroid gland2 = Thymus3 = Thymus Fetal4 = Tonsil5 = Tonsil6 = Lymph nodes Tumor7 = Marrow8 = Spinal Cord9 = Brain10 = Brain Fetal11 = H2O12 = Heart13 = Heart Fetal14 = Lung15 = Lung16 = Lung Tumor17 = Breast18 = Breast19 = Breast Tumor20 = Muscle21 = Muscle22 = Retina

RT-PCR: PRKWNK1,WNK1, T41 T42 (217 bp),Reamplifikation

040402_Reampli_Gewebe_1_A2_T41T42_calb

Bedingungen:2% TBE90V 1h 35min9 µ Reampli-Probe2 µl LB

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M

M 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M

M = Marker1 = Oesophagus2 = Stomach3 = Stomach Tumor4 = Corpus5 = Antrum6 = Small Intestine7 = Duodenum8 = Large Intestine9 = Large Intestine10 = Coecum11 = Coecum12 = Colon13 = Colon Tumor14 = Rectum15 = Rectum16 = Cervix17 = Endometrium18 = Uterus19 = Uterus Tumor20 = Ovary21 = Ovary Tumor22 = Ovary Tumor23 = Placenta24 = Testis

25 = Testis Tumor26 = Prostate gland27 = Prostate gland28 = Adrenal gland29 = Adrenal gland30 = Kidney31 = Kidney Fetal32 = Kidney Tumor33 = Kidney Tumor34 = Bladder35 = Spleen36 = Spleen37 = Spleen Fetal38 = Liver39 = Liver40 = Liver Fetal41 = Pancreas42 = Pancreas43 = Salivary gland

RT-PCR: PRKWNK1,WNK1, T41 T42 (217 bp),Reamplifikation

Bedingungen:2% TBE90V 1h 40min9 µl Reampli-Probe2 µl LB

040402_Reampli_Gewebe_2_A2_T41T42_calb

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M

M = Marker1 = Thyroid gland2 = Thymus3 = Thymus Fetal4 = Tonsil5 = Tonsil6 = Lymph nodes Tumor7 = Marrow8 = Spinal Cord9 = Brain10 = Brain Fetal11 = H2O12 = Heart13 = Heart Fetal14 = Lung15 = Lung16 = Lung Tumor17 = Breast18 = Breast19 = Breast Tumor20 = Muscle21 = Muscle22 = Retina