rtb-naro collaborations

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RTB-NARO Collaborations Uganda| 3 October 2014 Yona Baguma, PhD Acting Deputy Director General Research Coordination, NARO Plot 11-13,Lugard Avenue, P.O. Box 295, Entebbe, Uganda t: +256 (414) 320512, 320341/2 m: +256 (772) 930185, f: +256 (414) 321070 Email: [email protected] [email protected] ,

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Presentation at RTB Annual Review and Planning Meeting (Entebbe, Uganda, 29 Sep-3 Oct 2014)

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Page 1: RTB-NARO collaborations

RTB-NARO Collaborations

Uganda| 3 October 2014

• Yona Baguma, PhD• Acting Deputy Director General Research Coordination, NARO

Plot 11-13,Lugard Avenue, P.O. Box 295, Entebbe, Ugandat: +256 (414) 320512, 320341/2 m: +256 (772) 930185, f: +256 (414) 321070Email: [email protected] [email protected],

Page 2: RTB-NARO collaborations

WHAT IS NARO?

• Apex body responsible for guidance and coordination of all agricultural research activities in the NARS in Uganda

• Nonprofit, national

• Old NARO established in 1992 (NAR Act 1992)• New NARO established in 2005 (NAR Act 2005)

• About 834 staff

• NARO 16 PARI Centres (7 NARIs & 9 Zonal)

• Research activities conducted by semi- autonomous PARIs coordinated by a NARO Council Secretariat

Page 3: RTB-NARO collaborations

Biotechnology Applications to Combat Cassava Brown Streak

Disease• Aim: To develop and deploy new technologies to improve the

accuracy and efficiency of cassava breeding to address the cassava brown streak disease (CBSD) epidemic

• Duration: 1st Phase: 2009 to 2013; 2nd Phase 2014-2016

Key Achievements 36 new breeding materials that combine CBSD and CMD tolerance

developed Categorization of CBSD resistance/Tolerance Human capacity built for plant breeding: 2 MSc, 1 PhD.

Page 4: RTB-NARO collaborations

Cassava Varieties and Clean Seed to Combat CBSD and

CMD (5CP)• Aim: To exchange and evaluate 25 elite cassava genotypes

among NARS in CBSD affected countries, and to monitor degeneration due to CBSD in elite cassava varieties

Duration: 2012 – 2016

Key Achievements

Uganda received the 24 best CBSD tolerant varieties from the region

Experiment to study degeneration due to CBSD planted NARS Breeding Programmes:

Kenya, Tanzania, Malawi & Mozambique

Page 5: RTB-NARO collaborations

Double Haploid Breeding for Cassava Enhancement, Phase

IIAim: Development of robust protocols for generation of cassava double haploids

Duration: 2014 - 2017

Key Achievements

Androgenesis was undertaken primarily on two genotypes; all regenerated plantlets not haploid

Use of irradiated pollen tested at selected dosages; all regenerated plants not haploid

Page 6: RTB-NARO collaborations

Sweetpotato Action for Health and Security in Africa (SASHA)

Aim: Building of capacity in biotechnology and biosafety, and development of WRSP Varieties and Breeding Lines

Duration: 2009-2014

Key Achievements

• Protocol optimised for regeneration of adventitious shoots based on thidiazuron (TDZ)

• Optimized genetic transformation protocol to transform Cv Kyebandula

• Attempted to pyramid natural host resistance and genetically engineered resistance by transgenic breeding; F1 plants confirmed parent to progeny transfer of transgene

• 2 PhD students completed their studies

Page 7: RTB-NARO collaborations

Field Evaluation of Transgenic S/potato Expressing

siRNAse Protein For Resistance to SPVD

Achievements

Approval/Decision to conduct CFT granted by NBC 1st season evaluation completed, 2nd season planting done

Page 8: RTB-NARO collaborations

Keeping Disease-free Sweetpotato Planting Material Closer To

Farmers

Established 60 Net Tunnels on 30 farms

Page 9: RTB-NARO collaborations

Development and Dissemination of Biofortified Crops

9

Objective• Increase and/or introduce OFSP varieties in the

farming systems of Northern and Western Uganda for combating VAD.

Page 10: RTB-NARO collaborations

2 OFSP varieties officially released and are being disseminated by H+

SPK004 / 2006 / 1136

NASPOT 12 O (24.7t/ha)

NASPOT 7 /2006 /292

NASPOT 13 O(38.4 t/ha)

Page 11: RTB-NARO collaborations

Combating Banana Bacterial Wilt in Farming Communities

• Aim: Reduce the impact of BBW on farming communities in East and Central Africa using cultural methods

Key Achievements Fine tuned control package to incorporate single stem removal;

• Less laborious, No need for replanting; quick production recovery

• Used in TZ, DRC, Rwanda, Burundi and Kenya

Improved technology promotion;

• a combination of participatory and topdown approaches and effectively scaled out BBW control from village to regional level

• Reduced BBW prevalence from 42% to below 13% in 1 year in Ankole; with yield recovery worth US$ 54.4 million.

Page 12: RTB-NARO collaborations

Development of banana bacterial wilt (BBW) transgenic resistance

• Aim: To develop and deploy bananas resistant to BBW to address the epidemic that is very destructive and rapidly spreading with no source of resistance for classical banana improvement.

• Duration: 2nd Phase 2012-2016

Key Achievements 6 lines immune to BBW already being further evaluated in CFT at

NARL; to be evaluated in 2 other locations in Bulindi and Mbarara. Over 100 lines of 2 popular cultivars of M9 (with background

resistance to black sigatoka and Fusarium wilt) and Nakitembe developed with stacked genes of hrap and pflp – batch has material for commercial release in 2020

Human capacity built in molecular breeding; 1 students being trained at PhD.

Page 13: RTB-NARO collaborations

Banana Weevil and Nematode Transgenic Resistance

• Aim: To develop and deploy transgenic banana resistant for banana weevil and nematode resistance

• Duration: 2011-2015

Key Achievements Over 100 lines of Gonja and Nakitembe with resistance to both

weevils and nematodes developed and ready for screenhouse evaluation.

6 Embryogenic cell lines for Nakitmbe and 3 lines of Gonja developed

Page 14: RTB-NARO collaborations

Development of Matooke Resistant To Black Sigatoka

• Aim: To develop and deploy matooke resistant for black sigatoka

• Duration: 2011-2015

Key Achievements 2 hybrids in AYT on farmers’ fields before release 3 promising hybrids in PYT at NARL and on farmers’ fields 2 segregating populations for Fusarium wilt and weevils

developed Over 400 accessions in Musa germplasm serving the ECA

region Diploids developed for further matooke improvement

Page 15: RTB-NARO collaborations

Field evaluation of genetically modified potatoes for resistance to potato blight

disease caused by Phytophthora infestans at Kachwekano ZARDI

Aim: To evaluate two potato cultivars (Desiree and Victoria) over expressing the R genes; RB, Rpi-blb2 and Rpi-vnt1.1 in the field at Kachwekano, Kabale district in Uganda.

Duration: 2014-2016

Key Achievements•Greenhouse and field site contraction completed•Staff trained in Biosafety of CFTs•CFT application submitted to NBC•Mock trial with non-GM Desiree and Victoria cultivars completed

Page 16: RTB-NARO collaborations

Considerations of Increased Visibility of RTB in NARO

• Work jointly with RTB to identify, pilot, launch and commercialize research products to the market, including strong aspects of value addition.

• Proposal to have NARO-Directors and Team Leaders attend RTB business and/or enterprise planning meetings.

• Placement of RTB staff at both NARO research institutes and headquarters to jointly plan and implement pre-selected activities together.

• Use of new genomics approaches (genotyping by sequencing, comparative genomics, QTL mapping and gene discovery) to develop new varieties with yield and durable resistance value.

• Joint effort in establishing functional seed systems for Cassava, Sweet potato, and Banana