Supplementary Information

Electrochemiluminescence Detection of Human Breast Cancer Cells using Aptamer 3D

Printed Microchannel Bipolar Electrode

Hasan Motaghi, Sima Ziyaee, Masoud Ayatollahi Mehrgardi*, Abolghasem Abbasi Kajani, Abdol-Khalegh Bordbar

Department of chemistry, University of Isfahan, Isfahan, 81746-73441, Iran

∗ Corresponding author. Tel.: +98 311 7932710; fax: +98 311 6689732. E-mail addresses:

m.mehrgardi@sci.ui.ac.ir, m.mehrgardi@gmail.com (M.A. Mehrgardi).

Fig. S1 Nyquist plots for electrochemical impedance spectroscopy (EIS) data collected from the bare

or modified gold electrodes with 2µM AS1411 aptamer following different incubation time. All

measurements were recorded in a solution containing K3[Fe(CN)6]/K4[Fe(CN)6] (1 : 1, 0.5 mM) and

KCl (0.1 M). (impedance spectral frequency 0.1 – 10 kHz)

Fig. S2 EIS response for modified gold electrodes incubated with 1, 2and 3µM AS1411 aptamer in

PBS solution (pH 7.4)

Fig. S3 Ultraviolet-visible spectra of the gold nanoparticles before and after modification with aptamers

Fig. S4 Plots of ECL intensity of aptasensor in different concentrations of H2O2 solution (luminol:10

mM) 

Fig. S5 Dependence of ECL responses on pH of the detection solution (luminol: 10 mM and H2O2: 20 µM)