sabbatical leave — report of resultsmfey/gl/biotxcom/acs … · web viewinvestigation of protein...

BIOTDIVISION OF BIOCHEMICAL TECHNOLOGY Final Program, 234th ACS National Meeting, Boston, MA, August 19-23, 2007W. Chen, W. Wang, and A. S. Rathore, Program Chairs

SUNDAY MORNING

Section AUnknown Site -- Unknown RoomStem Cells: Quantifying, Modeling and Controlling Stem Cell Fate

J. M. Piret, Organizer8:20 —1. Behavior-activated cell selection: Application to stem cell expansion. B. Dykstra, J. Ramunas, D. Kent, C. J. Eaves, E. Jervis

8:50 —2. Tracking stem cell fate using a cell-based microscale platform. T. G. Fernandes, S. J. Kwon, M -Y. Lee, M. M. Diogo, C. Lobato da Silva, J. M. Cabral, J. S. Dordick

9:05 —3. Exploiting the synergy between lysate and acoustic standing wave fields to increase the retroviral transduction of suspension cells. P. R. Beauchesne, V. S. Tayi, B. D. Bowen, J. M. Piret

9:20 — Intermission.

9:40 —4. Self-assembly of multipotent adult progenitor cells (MAPCs) and differentiation to the hepatic lineage. K. Subramanian, K. Pauwelyn, C. Verfaillie, W -S. Hu

9:55 —5. 3-D differentiation model of ex vivo megakaryopoiesis. Y. Leysi-Derilou, N. Pineault, C. Duchesne, J -F. Boucher, A. Garnier

10:10 —6. P53 tumor suppressor protein affects hematopoietic stem cell differentiation in the megakaryocyte compartment. P. G. Fuhrken, P. Apostolidis, S. Lindsey, W. M. Miller, E. T. Papoutsakis

10:25 —7. Differentiation of adult human stem cells guided by mechano-sensing of matrix elasticity. F. Rehfeldt, A. J. Engler, D. E. Discher

Section BUnknown Site -- Unknown Room

Biophysical and Biomolecular Symposium: Protein Stability

E. J. Fernandez, N. Rathore, C. R. Middaugh, A. Blake-Haskins, and A. Blake-Haskins, Organizers8:00 — Introductory Remarks.

8:05 —8. Use of biophysical techniques for manufacturability assessment of therapeutic proteins. C. Li, Y. Jiang, J. Svitel, J. Wen, R. Ramachander, S. Wang, J. Li, L. Narhi

8:25 —9. A novel bioseparation approach: Use of chaotropic agents for HIC and ion exchange chromatography to dissociate noncovalent multimers during purification of an Fc fusion protein. A. B. Magill II

8:45 —10. Aggregation of a monoclonal antibody induced by adsorption to microparticle surfaces. J. Bee, J. Stevenson, K. Chatterjee, E. Freund, J. F. Carpenter, T. W. Randolph

9:05 —11. Assessment of protein stability during freeze-thaw process. K. Gupta, N. Rathore, L. Barron, W. Ji, F. Jameel, K. Murphy


9:45 —12. Identifying optimal solution conditions for high concentration protein formulations: Use of self-interaction chromatography. M. C. Manning, C. S. Henry, J. J. Valente, R. W. Payne, W. W. Wilson

10:05 —13. Stability of high concentration rhuMAb VEGF. H. Liu, M. E. M. Cromwell

10:25 —14. Stability of protein antigens in vaccine delivery formulations. J. D. Chesko, J. Kazzaz, P. Malyala, K. Patton, M. Ugozzoli, D. O'Hagan, M. Singh

10:45 —15. Tungsten, prefilled syringes and protein aggregation. R. Swift, Y. Nashed-Samuel, W. Liu, L. Narhi, J. Davis

11:05 — Concluding Remarks.

Section CUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Protein Folding & Characterization

K. Mallela and T. Cellmer, Organizers8:00 —16. Hidden folding intermediates in small proteins: Implications for the folding energy landscape, cooperativity, and evolution of protein structures. Y. Bai

8:25 —17. Electrostatic properties of the unfolded state: What can we learn from modeling? N. C. Fitzkee, B. García-Moreno E

8:45 —18. Ultrafast folding of the villin subdomain. T. Cellmer, W. A. Eaton, J. Hofrichter

9:05 —19. Simultaneous and systematic evaluation of multiple protein refolding conditions. P. A. Leland


9:45 —20. Driving forces for protein folding on the ribosome as a function of polypeptide chain elongation: Experimental and computational approaches. S. Cavagnero, J. P. Ellis, B. C. Mounce, N. Kurt

10:10 —21. Joining NMR and simulation to capture Alzheimer's Aβ monomer and fibril structural ensembles. N. L. Fawzi, T. Head-Gordon

10:30 —22. Denaturant and isotopic effects on stability and folding of interleukin-1 receptor antagonist. R. F. Latypov, D. Liu, T. Kohno, D. N. Brems, A. A. Raibekas

10:50 —23. Protein folding principles: Foldons, sequential stabilization, and optional errors. K. Mallela, S. W. Englander

Section DUnknown Site -- Unknown RoomUpstream Processing: Advances in Biocatalysis

P. C. Cirino and L. Sun, Organizers8:00 —24. A blue fluorescent protein with oxidoreductase activity. K. M. Polizzi, D. A. Moore, A. S. Bommarius

8:20 —25. A novel laccase/redox mediator system increases space-time yields and operational stability in dehydrogenase reactions. R. Ludwig, D. Haltrich

8:40 —26. Alginate-derived nanovolume cytochrome P450 microarrays for high-throughput inhibition assays. S. M. Sukumaran, M -Y. Lee, D. S. Clark, J. S. Dordick

9:00 —27. Evolution in reverse: Engineering a xylose-specific xylose reductase. N. Nair, H. Zhao


9:40 —28. Directed evolution of LuxI for enhanced synthesis of OHHL. P. K. R. Kambam, L. Sun

10:00 —29. Biocatalytic systems for aromatic oxidations. A. M. McIver, S. V. B. J. Garikapati, T. L. Peeples

10:20 —30. Design of an enzyme-chaperone chimera as a new approach to enzyme immobilization and self-renaturation. L. M. Bergeron, T. Tokatlian, D. S. Clark

10:40 —31. Protein dissection and shuffling for generating recombined proteins. Y. Chen, T. Chen, H. Hua, Z. Lin

Section EUnknown Site -- Unknown RoomAlan S. Michaels Recovery Award Lecture

W. Zhou, Organizer11:30 — Introductory Remarks.

11:35 —32. Early steps in protein recovery: The messy part of a clean industry. M -R. A. Kula

SUNDAY AFTERNOON

Section AUnknown Site -- Unknown RoomStem Cells: Stem Cell Based Tissue Engineering

T. McDevitt, T. McDevitt, and P. W. Zandstra, Organizers2:00 —33. Generation and characterization of keratinocytes from human embryonic stem cells. C. M. Metallo, L. Ji, J. J. De Pablo, S. P. Palecek

2:30 —34. Characterization of embryonic stem cells' differentiation into insulin-producing endocrines cells in 3-D cultures. X. Wang, K. Ye

2:45 —35. A novel method to control differentiating human embryonic stem cell (hESC) aggregate size and composition: Effects on cardiomyocyte development. C. L. Bauwens, R. Peerani, S. Niebruegge, K. Kolodziejska, M. Husain, P. W. Zandstra


3:15 —36. Serum-free expansion of embryonic stem cells in a bioreactor for tissue engineering applications. D. E. Kehoe, L. T. Lock, E. S. Tzanakakis

3:30 —37. Hypoxic effect on hematopoietic development in human embryonic stem cell-derived embryoid bodies. C. M. Cameron, W -S. Hu, D. S. Kaufman

Section BUnknown Site -- Unknown Room

Downstream Processing: Primary Recovery - Membranes and Other Technologies

D. J. Roush and Y. Lu, Organizers2:00 — Introductory Remarks.

2:05 —38. Engineering the adsorption of plasmid DNA. W. J. Kelly, P. Butts

2:25 —39. Creating a robust and reproducible recovery process using centrifugation for the recovery of a soluble fusion protein in Saccharomyces cerevisiae. K. Herzog, J. Barringer, C. Brady

2:45 —40. Ideal membrane cascades for downstream processing. E. N. Lightfoot, J. L. O'Dell


3:15 —41. Optimization of cell culture harvest clarification using an ultrascale down model for centrifugation. M. Menon, A. Hamilton, F. Riske

3:35 —42. Effect of solid ejection size on continuous centrifugation during mammalian cell culture product recovery. X. Zhao, J. Zhou

3:55 —43. Case study: Optimization of harvest stream clarification steps of a commercially-relevant fusion protein mammalian cell culture using charged depth filters. R. Bates, J. Ma, C. Mason

Section CUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Protein Folding & Characterization

K. Mallela and T. Cellmer, Organizers2:00 —44. All-atom computer simulation of amyloid fibrils disaggregation. C. Tan, H -F. Chen, R. Luo

2:25 —45. Dynamics of intramolecular contact formation in islet amyloid polypeptide (IAPP). S. Vaiana, W. A. Eaton, R. Ghirlando, J. Hofrichter

2:45 —46. Folding of the β-helix domain of P22 tailspike protein. M. L. Spatara, C. J. Roberts, A. S. Robinson


3:15 —47. Folding and insertion of melittin in membrane mimics. M. R. Hartings, H. B. Gray, J. R. Winkler

3:35 —48. Understanding the molecular mechanism of Hsp90: The role of nucleotide hydrolysis in the chaperone cycle. K. A. Krukenberg, F. Foerster, A. Sali, D. A. Agard

3:55 —49. Breaking the symmetry of a homodimeric enzyme: Engineering and characterization of monomeric disulfide isomerases. S. A. Arredondo, G. Georgiou

Section DUnknown Site -- Unknown RoomEmerging Technologies: NanobiotechnologyCosponsored with ANYL, and BIOHWJ. R. Cochran and L. Malmberg, Organizers2:00 —50. Co-assembly of genetically-modified bacteriophages and inorganic nanoparticles into silica-stabilized nanoarchitectures. C. E. Ashley, E. C. Carnes, L. T. White, Z. Yuan, D. R. Dunphy, D. N. Petsev, P. Atanassov, D. Peabody, C. J. Brinker

2:20 —51. Biofabrication with genetically modified viral nanotemplates. H. Yi, G. W. Rubloff, G. F. Payne, W. E. Bentley, J. N. Culver

2:40 —52. Engineering stability and self-assembly in a caged protein scaffold. S. Lim, M. Dalmau Mallorqui, C. I. Ruiz, S -W. Wang


3:10 —53. Nanoscale reservoirs for spatially and temporally controlled biointerfaces. N. A. Melosh, J. J. VanDersarl, E. Hagar-Barnard, E. Yenilmez

3:30 —54. Biological fabrication of nanostructured silicon-germanium photonic crystals possessing unique photoluminescent and electroluminescent properties. G. L. Rorrer, C. Jeffryes, D. Lee, T. Qin, C -H. Chang, T. Gutu, J. Jiao, R. Solanki

3:50 —55. Biologically programmed synthesis of hybrid semiconductor nanocrystals. S. Singh, N. Myung, A. Mulchandani, W. Chen

Section EUnknown Site -- Unknown RoomCommercialization of Biologics: Case Studies: Post-Approval Changes & Comparability Challenges

S. Lee and V. Paradkar, Presiding2:00 — Introductory Remarks.

2:05 —56. Post approval changes for a commercial process: An historical look. C. Heath, H. Madani

2:25 —57. Case study: Downstream purification support of a cell culture raw material change for a licensed mAb product. F. Maslanka

2:45 —58. Rituximab post approval changes and comparability assessments. R. Kiss, S. R. Chary, S. Meier, J. C. Goodrick, D. C. Andersen


3:15 —59. Demonstrating comparability for post approval CMC changes. R. Harris

3:35 —60. Comparability protocol to support a chromatography resin change. P. J. Kramer, R. Frankenberg, R. Bhat, P. Burd, A. Hetherington

3:55 —61. Investigation of an ultrafiltration yield problem for a conjugate vaccine. S -M. Lee, B. Kruse, A. Robinson, J. Davis, C. Donaldson


Section FUnknown Site -- Unknown RoomPerlman Lecture

W. Zhou, Organizer4:30 —62. Prion proteins and how they fold. S. Lindquist

Chemical Approaches to Protein FunctionSponsored by BIOL, Cosponsored with ANYL, BIOT, MEDI, BTEC, and BIOHW

MONDAY MORNING

Section AUnknown Site -- Unknown RoomEmerging Technologies: NanobiotechnologyCosponsored with ANYL, and BIOHWJ. R. Cochran and L. Malmberg, Organizers8:00 —63. Inhibiting bacterial biofilm formation by self-assembled monolayers of functional alkanethiols on gold. S. Hou, E. A. Burton, K. A. Simon, D. Blodgett, Y -Y. Luk, D. Ren

8:20 —64. Development of electrospun scaffolds for application to medical devices. R. Delaporte, H. Yesilalan, R. Skowyra, G. Cadd

8:40 —65. Polymersome encapsulated hemoglobin: A novel type of hemoglobin-based

oxygen carrier. A. F. Palmer

9:00 —66. A novel, biomimetic hydrogel construct to repair the cornea: Molecular design and biological response. D. Myung, J. R. Cochran, J. Noolandi, C. N. Ta, C. W. Frank


9:35 —67. Multifunctional titanium-binding peptides for rapid modification of implant surfaces. X. Khoo, D. J. Kenan, M. W. Grinstaff

9:55 —68. Effects of platinum and gold nanoparticles on osteoblast cells: A study of nanoparticles' cancer treatment potential. Y. Sun, D. Katz, T. Fraczak, N. Pernodet, M. Rafailovich, J. Sokolov

10:15 —69. Degradable nanoparticle gene carriers for efficient and versatile nonviral gene delivery. S. Lu, Y. J. Kwon

10:35 —70. Assembly of PEI/DNA nanoparticles for gene delivery by microfluidic hydrodynamic focusing. C -G. Koh, Z. Fei, J. Guan, Y. Xie, L. J. Lee

Section BUnknown Site -- Unknown RoomDownstream Processing: Advances in Chromatography

A. M. Lenhoff and S. Ghose, Organizers8:00 —71. Are high capacity and high selectivity mutually exclusive? New approaches to the chromatographic resin game. J. Rasmussen

8:20 —72. Microstructure and retention properties of dextran-grafted agarose media. H. Koku, B. D. Bowes, A. M. Lenhoff

8:40 —73. Use of displacement chromatography for proteomic applications. S. T. Evans, A. S. Freed, M. Platt, S. M. Cramer

9:00 —74. High performance chromatofocusing as a novel analytical method to support process comparability and product characterization. X. Kang, J. Kutzko, M. Hayes, D. Frey


9:40 —75. Membrane adsorber technology for trace impurity removal applications. M. W. Phillips, C. Teske, R. Specht, M. Kozlov, W. Moya

10:00 —76. Validation of host cell protein removal in purification processes of biopharmaceutical manufacturing. C. Jiang, J. Ma, M. Rubacha, S. Lee, A. A. Shukla

10:20 —77. Profiling metal binding E. coli proteins: Its use in purification process development. R. Varakala, R. Haley, R. Henry, M. M. Ataai, R. R. Beitle

10:40 —78. Affinity adsorption of viruses using small peptide ligands. C. L. Heldt, P. V. Gurgel, L -A. Jaykus, R. G. Carbonell

Section CUnknown Site -- Unknown RoomUpstream Processing: Advances in Microbial Fermentation Process Development

D. Ren, M. Laird, and X. Yang, Organizers8:00 — Introductory Remarks.

8:10 —79. Alleviation of extracytoplasmic stress for improving cell physiology and recombinant protein production in Escherichia coli. C. P. Chou

8:30 —80. Metabolic activities of novel inducible fermentations for recombinant therapeutic proteins. M. Berge, R. Georgescu

8:50 —81. Fast track first-in-human development of novel therapeutic proteins with a robust microbial fermentation platform. X. Yang, B. Pendleton

9:10 —82. Using the two population theory to design an inducer feeding schedule to maximize recombinant DNA protein expression in Escherichia coli. J. M. Gonzalez, J. T. Hsu

9:30 —83. Mixture design for optimization of fermentation medium for cutinase production from Colleotrichum lindemuthianum. V. Shah, F. J. Rispoli

9:50 —84. Efficient antibody production with suppressing O-Glycosylation in yeast. K. Kobayashi, K. Kuroda, H. Tsumura, T. Komeda, Y. Chiba, Y. Jigami, K. Ichikawa, K. Nonaka, T. Suzuki


Section DUnknown Site -- Unknown RoomUpstream Processing: Advances in Biocatalysis

P. C. Cirino and L. Sun, Organizers8:00 —85. Efficient synthesis of simvastatin using whole-cell biocatalysis. Y. Tang, X. Xie

8:20 —86. Enzyme-carbon nanotube conjugates in room temperature ionic liquids. B. Eker, P. Asuri, S. Murugesan, R. J. Linhardt, J. S. Dordick

8:40 —87. Exploiting phenotypic diversity through global transcription machinery engineering. D. Klein-Marcuschamer, G. Stephanopoulos

9:00 —88. High-throughput zcreening for methionyl-tRNA synthetases that enable residue-specific incorporation of noncanonical amino acids into recombinant proteins in bacterial cells. T. H. Yoo, D. A. Tirrell


9:35 —89. What controls enantioselectivity for carbonyl reductase. L. Hua, D. Zhu, Y. Yang, T. H -Y. Pan

9:55 —90. Hydration effects on the active site and catalytic performance of organic-soluble subtilisin in octane. E. P. Hudson, J. S. Dordick, J. A. Reimer, D. S. Clark

10:15 —91. Synthesis of anti-obesity stilbene derivatives using engineered microbial biocatalysts: Structure-function analysis of -glucosidase inhibition potency. E. Leonard, K -H. Lim, C -G. Lim, J. Chemler, M. Koffas

10:35 —92. Biotransformations on solid-supported substrates. S. Brooks, L. Coulombel, U. Akbar, D. S. Clark, J. S. Dordick

Section EUnknown Site -- Unknown RoomIndustrial Biotechnology Award Lecture

W. Zhou, Organizer11:30 —93. Genentech Lucentis® Development CMC team: "A vision saving therapy made by biotechnology". J. C. Joly

Genomics of ObesitySponsored by BIOHW, Cosponsored with AGFD, BIOL, BIOT, MEDI, and BTEC

Pfizer Award SymposiumSponsored by BIOL, Cosponsored with ANYL, BIOT, MEDI, BTEC, and BIOHW

MONDAY AFTERNOON

Section A

Unknown Site -- Unknown RoomStem Cells: Engineering the Embryonic and Adult Stem Cell Niche

A. Khademhosseini and B. Rao, Organizers3:00 —94. Matrix and environmental control of stem cell outcomes for tissue engineering. D. L. Kaplan, H. J. Kim, X. Wang, X. Wang, C. Vepari, A. Murphy, I. Georgakoudi

3:30 —95. The effect of cell density and oxygen level on culture for rodent multipotent adult progenitor cells (MAPCs). Y. Park, F. Ulloa, C. Verfaillie, W -S. Hu

3:45 —96. cAMP induced neural differentiation of mesenchymal stem cells: Real differentiation or pseudo-differentiation? L. Zhang, C. Chan

4:00 —97. Effects of scaffold porosity and pore size on cell entrapment, attachment, distribution and proliferation in 3-D cell culture system. R. Ng, S -T. Yang


4:30 —98. Ex vivo expansion of human hematopoietic stem/progenitor cells in a human stromal-based culture system. C. Lobato da Silva, R. Goncalves, M. Lemos, F. Lemos, G. Almeida-Porada, J. Cabral

4:45 —99. Multiparameter flow cytometric assay to monitor the activation of cytokine signaling pathways in specific subsets of primary erythroid cells. W. Wang, J. Audet

Section BUnknown Site -- Unknown RoomDownstream Processing: Bioprocess Integration & Industrial Case-Studies

M. R. Ladisch and C. Liu, Organizers3:00 —100. CSAF: Constant shear affinity filtration technology. P. Francis, E. von Lieres, F. Taghipour, B. D. Bowen, C. A. Haynes

3:20 —101. Production of enantiopure molecules by integration of SMB technology and biocatalysis. M. Bechtold, S. Makart, S. Panke

3:40 —102. Considerations for large scale extraction of monoclonal antibodies targeted to different subcellular compartments in transgenic tobacco plants. S. Hassan, C. van Dolleweerd, J. Ma, W. Liu, C. R. Thomas, E. Keshavarz-Moore


4:20 —103. Evaluating and minimizing water usage and wastewater ceneration in integrated bioprocesses using process simulation and scheduling tools. D. Petrides, C.

Siletti

4:40 —104. Development and implementation of a manufacturing scale virus filtration step for monoclonal antibody production. J. F. Hsii, C. Dowd

5:00 —105. Humira after launch: Opportunities and challenges in downstream process. M. Wan, F. Gaibor, M. Schrader, R. M. Boychyn, E. Lundell, G. Avgerinos

Section CUnknown Site -- Unknown RoomUpstream Processing: Advances in Microbial Fermentation Process Development

X. Yang, M. Laird, and D. Ren, Organizers3:00 — Introductory Remarks.

3:05 —106. "Seeing the light" with cell-free protein synthesis. A. R. Goerke, A. M. Loening, S. Gambhir, J. R. Swartz

3:25 —107. Enhancing extracellular protein secretion in Escherichia coli by translation engineering. P. Gupta, P. S. Lee, K. H. Lee

3:45 —108. Developing high throughput screening methods for multiple phenotype evaluation. B. Wang, Y. Zhang, G. N. Stephanopoulos

4:05 —109. Metabolic and process engineering of Propionibacterium acidipropionici for enhanced propionic acid fermentation. A. Zhang, S -T. Yang

4:25 —110. Production of sophorolipid from cooking oil waste through fermentation. V. Shah

4:45 — Panel Discussion.


Section DUnknown Site -- Unknown RoomCommercialization of Biologics: Data Management & Process Modeling in Support of Commercial Processes

J. Prior and S. Ahuja, Organizers3:00 —111. Multivariate analysis of transition analysis calculations applied to evaluate trends and changes in chromatography column packed bed integrity at BiogenIdec. B. Gilbert

3:20 —112. Application of multivariate analysis toward biotech processes: Case study of a cell-culture unit operation. A. S. Rathore, A. Kirdar

3:40 —113. Production planning, scheduling, and debottlenecking practices in the biopharmaceutical industries. C. Siletti, D. Petrides


4:20 —114. Case study: Applying the Six Sigma methodology to a commercial cell culture process. R. Davis, S. Ahuja, M. Herwig, K. Hwang

4:40 —115. Strategies for integrating spectral data from raw materials to control biopharmaceutical manufacturing. J. Wei, M. Kiistala, M. Lanan, J. Ganguly

5:00 —116. Use of mathematical models to determine the effect of various raw materials on mammalian cell culture performance at commercial-scale. S. Jain, B. Schilling, A. A. Shukla, S. Lee

Section EUnknown Site -- Unknown RoomIndustrial Keynote Symposium

W. Chen, Organizer, Presiding2:00 —117. Keynote Address. B. Adamson


Nano-Biochemistry and TechnologySponsored by ANYL, Cosponsored with BIOT, BIOHW, BTEC, and BIOL

Undergraduate Research Poster Session

BiochemistrySponsored by CHED, Cosponsored with BIOT, SOCED, and BIOL

TUESDAY MORNING

Section AUnknown Site -- Unknown RoomEmerging Technologies: Bioenergy

M. R. Ladisch and C. E. Wyman, Organizers8:00 —118. Commercialization of biomass processing: A consolidated approach. M. Moniruzzaman

8:20 —119. Comparative hydrolysis, fermentation, and economic information for application of leading pretreatment technologies to corn stover and poplar. C. E. Wyman, B. Yang, B. E. Dale, R. Elander, M. Holtzapple, M. R. Ladisch, Y. Y. Lee, C. Mitchinson, J. N. Saddler

8:40 —120. Mechanisms of plant cell wall deconstruction as a function of pretreatment and enzyme hydrolysis. M. Zeng, N. Mosier, C. E. Wyman, M. R. Ladisch

9:00 —121. Efficient ethanol production with engineered microorganisms. C. T. Trinh, F. Srienc


9:40 —122. Two-stage continuous fermentation of cellulose to ethanol. C. Liu, J. Bardsley, C. E. Wyman

10:00 —123. Butanol fermentation by Clostridium acetobutylicum immobilized in a fibrous bed bioreactor. W -L. Chang, S -T. Yang

10:20 —124. Enzymatic synthesis of biomass-based fuel methanol. P. Wang, F. S. Baskaya, M. C. Flickinger

10:40 —125. Long-term hydrogen production from robust nongrowing Rhodopseudomonas palustris coatings and strategies for increased hydrogen production. J. L. Gosse, B. J. Engel, A. S. Al-Homoud, C. S. Harwood, M. C. Flickinger

Section BUnknown Site -- Unknown RoomDownstream Processing: Advances in Chromatography

A. M. Lenhoff and S. Ghose, Organizers8:00 —126. Post Protein A removal of contaminants from monoclonal antibodies with a multimodal anion exchanger. K. Eriksson, A. Ljunglöf, G. Rodrigo

8:20 —127. Removal of HMW species using AEX in the weak partitioning mode for antibodies and related proteins. S. A. Tobler, A. Noyes, P. R. Brown, M. Switzer, B. Kelley

8:40 —128. Advances in membrane affinity chromatography for the recovery of antibodies. C. Boi, S. Dimartino, G. C. Sarti

9:00 —129. The use of modeling tools for the efficient purification process development. D. Dong, T. Gervais, D. Tran, N. Quinlan, A. Velayudhan, P. Alred, P. Alfonso


9:40 —130. Modeling-based rational approach to purify proteins: A case study. B. K. Nfor, T. Ahamed, M. Ottens, E. J. A. X. van de Sandt, M. H. M. Eppink, G. W. K. van Dedem, L. A. van der Wielen

10:00 —131. Investigation of protein binding in ion exchange systems with protein charge ladders and homologous protein libraries. W. K. Chung, Y. Hou, J. J. Keba, G. Makhatadze, S. M. Cramer

10:20 —132. Analysis and prediction of protein adsorption isotherms from interaction data. X. Xu, A. M. Lenhoff

Section CUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Protein Chemical Instability

E. M. Topp and M. Cromwell, Organizers8:00 — Introductory Remarks.

8:05 —133. Chemical stability of proteins in injectable drug delivery systems. F. Okumu

8:25 —134. Fragmentation within human IgG1 hinge region: Two distinct mechanisms, two different outcomes. J. Vlasak, C. E. Price, S. L. Cohen

8:45 —135. Gamma irradiation-induced damage in proteins and peptides. S -X. Xie, T. D. Williams, D. Willey, E. M. Topp

9:05 —136. Impact of degradations on bioactivity: A reflection from a monoclonal antibody. B. L. Yu, A. Vizel, M. Young, A. Morando, B. He


9:45 —137. Selective protein oxidation through reactive oxygen species: Catalysis by cysteine. C. Schoneich

10:05 —138. Using solution NMR to probe the effects of excipients on protein stability. J. S. Laurence

10:25 —139. Effect of residual moisture content on the stability of dried Apo2L/TRAIL crystals. H. Flores, A. S. Ressurreiçao, B. Zhang, B. Kabakoff, Z. Shahrokh, M. E. M. Cromwell

10:45 —140. Protein stability at solid substrates: Influence of chemical and thermal excursions. G. Anand, G. Belfort

Section DUnknown Site -- Unknown RoomUpstream Processing: Advances in Metabolic Engineering

J. March and I. S. Aldor, Organizers8:00 — Introductory Remarks.

8:05 —141. Application of metabolic flux and logarithmic sensitivity analysis for optimization of A. niger medium. R. Gheshlaghi, J. M. Scharer, M. Moo-Young

8:25 —142. Proteome map of Aspergillus nidulans during osmoadaptation. Y. Kim, M. P. Nandakumar, M. R. Marten

8:45 —143. Nanofactories for synthesis and delivery of signaling molecules: A tool for engineering metabolism. R. Fernandes, C -Y. Tsao, C. W. Yung, W. E. Bentley

9:05 —144. Engineering signal transduction for treating type 1 diabetes. J. March, F. Duan


9:45 —145. Development of a melanin-based screen for tyrosine production in Escherichia coli. C. N. S. Santos, G. N. Stephanopoulos

10:05 —146. Engineering synthetic pathways for production of butanol as biofuels. S. Atsumi, J. C. Liao

10:25 —147. Intracellular enzyme crosslinking: Toward multifunctional enzyme machines for the production of R-1,2-propanediol. R. J. Conrado, M. DeLisa

10:45 —148. Toward microbial synthesis of glucaric acid. T. S. Moon, A. Lanza, L. Octavio, K. Jones Prather

Section EUnknown Site -- Unknown RoomMarvin J. Johnson Award Lecture

W. Zhou, Organizer11:30 —149. Molecular bioprocessing from design to discovery to dreams. J. S. Dordick


RNA Interference Based TherapeuticsSponsored by CARB, Cosponsored with BIOL, BIOT, COMP, MEDI, ORGN, PMSE, POLY, and BTEC

TUESDAY AFTERNOON

Section AUnknown Site -- Unknown RoomEmerging Technology Keynote Lecture

H. Zhao and R. Srivastava, Organizers2:00 — Introductory Remarks.

2:05 —150. Energy from biomass: Anticipating a revolution. L. R. Lynd

Section BUnknown Site -- Unknown RoomUpstream Processing: Advances in Cell Culture Process Development

S. T. Sharfstein, I. Blumentals, and G. Maheshwari, Organizers3:00 — Introductory Remarks.

3:05 —151. Leveraging process characterization and validation data for continuous process improvement. M. Luu, R. Kiss, S. Meier

3:25 —152. Inhibitory threshold values of lactate, ammonia, carbon dioxide, and osmolality in CHO cell culture for a therapeutic protein production. Z. Xing, Z. Li, V. Chow, S. S. Lee

3:45 —153. Scale-up of mammalian cell cultures controlled by automated flow cytometry. G. Sitton, F. Srienc


4:20 —154. Metabolic process engineering applied to cell culture production processes. B. M. Schilling, A. A. Shukla, S. Lee

4:40 —155. Investigating limitations during process development at reduced perfusion rates. H. Drouin, S. Lanthier, A. Kamen, J. M. Piret, Y. Durocher

5:00 —156. High-throughput cell culture process development using 3-D microbioreactors and online quantifications of cell growth and GFP expression. X. Zhang, Y. Wen, S -T. Yang


Section CUnknown Site -- Unknown RoomDownstream Processing: Molecular Interactions and Modeling Approaches

J. Thommes and K. M. Lacki, Organizers3:00 —157. Custom chromatography: Optimizing surface functionalization to meet specific separation needs. C. A. Haynes

3:20 —158. Molecular dynamic investigation of the interaction of supported affinity ligands with monoclonal antibodies. L. Zamolo, V. Busini, D. Moiani, D. Moscatelli, C. Cavallotti

3:40 —159. Construction and study of porous polymer media for ion-exchange adsorption by molecular dynamics modeling and simulations. E. Riccardi, J. -C.Wang, A. I. Liapis


4:20 —160. Investigation of ligand-protein binding affinity in ion exchange chromatography. T. Yang, W. K. Chung, S. A. McCallum, J. Kempf, S. M. Cramer

4:40 —161. Molecular level inspection of viral clearance and membrane adsorbers. W. T. Riordan, M. R. Etzel

5:00 —162. Modeling of protein monomer/aggregate separation using Hydrophobic Interaction Chromatography: Effects of column scale. J. T. McCue

Section DUnknown Site -- Unknown RoomCommercialization of Biologics: Impact of Design Space Philosophy & Emerging Regulations on Process Validation

G. Knipp, K. Webber, and A. Kataria, Organizers3:00 —163. Efficient approach to setting validation acceptance criteria for a biopharmaceutical process. T. Gleason, R. Burdick, S. Rausch, J. E. Seely

3:20 —164. A systematic method for design space definition. R. D. Hegedus

3:40 —165. Apply design space philosophy in the establishment of process validation parameters and acceptance criteria. X. Wang, J. Harms, P. Van Hoek, A. S. Rathore


4:20 —166. Quality by design and process analytical technology for freeze drying. M. Pikal

4:40 —167. Critical process parameters and design space. P. W. Wojciechowski

5:00 —168. Use of online Process Analytical Technology (PAT) to monitor Maytansinoid conjugation to modified antibodies: Implications in establishing robust processes for manufacturing disulfide-linked immunoconjugates. I. M. Schwartz, S. Gohain, D. C. Pentia, G. Amphlett, D. H. Meshulam, G. Malin

Section EUnknown Site -- Unknown RoomUpstream Processing: Advances in Metabolic Engineering

J. March and I. S. Aldor, Organizers3:00 —169. Incorporation of thermodynamics and metabolomics data into constraint-based model of Methylobacterium extorquens AM1. R. E. Osterhout, M. van der Werf, S. Van Dien

3:20 —170. Metabolic engineering of natural and unnatural flavonoid biosynthesis in microorganisms and their application for diabetes treatments. J. Chemler, M. Koffas, E. S. Tzanakakis, Y. Yan, E. Leonard, Z. Fowler, L. T. Lock

3:40 —171. Metabolic engineering of Saccharomyces cerevisiae for enhanced uptake and sequestration of arsenic. M. Shen, S. Singh, D. Shah, W. Lee, W. Chen, N. A. Da Silva

4:00 —172. Metabolic flux elucidation for large-scale models: 13C labeled isotope experiments and experimental design. P. F. Suthers, A. P. Burgard, M. S. Dasika, F. Nowroozi, S. Van Dien, J. D. Keasling, C. D. Maranas


4:30 —173. Biosynthesis of fosfomycin. Z. Shao, R. Woodyer, H. Zhao

4:50 —174. Probing the supply-side attributes of different metabolically engineered host backgrounds. D. Cunningham, N. Domagalski, R. Koepsel, M. M. Ataai, M. M. Domach

5:10 —175. Growth-phase accumulation of poly-3-hydroxybutyrate in Escherichia coli:

A prospectus for continuous production. K. E. Tyo, G. N. Stephanopoulos



Mechanism of Action of Natural ProductsSponsored by BIOL, Cosponsored with BIOT, MEDI, ORGN, BTEC, and BIOHW

Molecular Imaging

Visualization of Biological Processes at the Molecular LevelSponsored by BIOL, Cosponsored with ANYL, BIOT, MEDI, BTEC, PHYS, and BIOHW

RNA Interference Based TherapeuticsSponsored by CARB, Cosponsored with BIOL, BIOT, COMP, MEDI, ORGN, PMSE, POLY, and BTEC

WEDNESDAY MORNING

Section AUnknown Site -- Unknown RoomEmerging Technologies: Systems BiologyCosponsored with BIOHWC. Chan and C. Cho, Organizers8:00 —176. Scaffold proteins tune the regulatory properties of protein kinase cascades. J. W. Locasale

8:20 —177. Molecular systems biology via multiscale modeling and high-performance computing. J. Purvis, Y. Liu, A. Shih, N. Agrawal, R. Radhakrishnan

8:40 —178. Predicted points of cell-cycle fragility are consistent with known malfunctions in solid and hematological cancers. S. Nayak, S. Salim, J. D. Varner

9:00 —179. Transcription analysis with gene expression and network connectivity data. M. P. Brynildsen, L. M. Tran, T -Y. Wu, S -S. Jang, J. C. Liao


9:40 —180. Genome-scale metabolic analysis of bacteriophage infected Escherichia coli. R. Jain, R. Srivastava

10:00 —181. Elucidating and filling gaps in genome-scale metabolic networks. V. Satish Kumar, M. S. Dasika, C. D. Maranas

10:20 —182. Metabolic network inference based on probabilistic modeling of metabolic profiles. J. Yoon, K. Lee

10:40 —183. Isotopically nonstationary flux analysis using an elementary metabolite unit (EMU) framework. J. L. Walther, J. D. Young, M. R. Antoniewicz, H. Yoo, G. Stephanopoulos

Section BUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Targeted Delivery of Proteins & Nucleotides

C. Meares, W. Liu, and A. Blake-Haskins, Organizers8:00 — Introductory Remarks.

8:05 —184. (R-Ahx-R)4 cell-penetrating peptide enhances the in vivo delivery of antisense morpholino oligomers. H. M. Moulton, N. Jearawiriyapaisarn, S. Fletcher, B. W. Neuman, R. Kole, W. Steve, M. Buchmeier, P. L. Iversen

8:25 —185. Mutant glycosyltransferases assist in linking glycoconjugates via glycan chains: Development of a targeted drug delivery system and contrast agents for MRI. P. K. Qasba, E. Boeggeman, B. Ramakrishnan

8:45 —186. A new gene delivery method based on yeast cell wall particles. E. Soto, G. Ostroff

9:05 —187. Reversing the nucleophile and electrophile for covalent affinity capture. D. D. Singh, M. R. McCoy, Z. Miao, O. L. Hsu, C. Meares


9:40 —188. D-galactose receptor-targeted in vivo spectral fluorescence molecular imaging of peritoneal metastasis. H. Kobayashi, Y. Hama, A. J. Gunn, Y. Koyama, Y. Urano, P. L. Choyke

10:00 —189. Design of novel non-viral gene vector based on head-tail type polycation block copolymer. A. Harada, M. Kawamura, Y. Kimura, C. Kojima, K. Kono

10:20 —190. In vivo RNAi: New concepts and applications of siRNA-based therapeutic agents. T. M. Rana

10:40 —191. Potent immunoconjugates for cancer therapy. P. D. Senter

Section CUnknown Site -- Unknown RoomCommercialization of Biologics: Characterization and Improvement of Platforms to Aid Commercialization of Biologics

S. Gadam and F. Olsen, Organizers8:00 —192. A systems approach to characterization, validation and monitoring of commercial processes. A. A. Shukla, S. Lee

8:20 —193. Systematic and logical strategies to reduce process scale-up and technology transfer challenges from process development to manufacturing. T -S. Lee, M. McGill, P. Thompson

8:40 —194. Evaluation of process and product impact of UVC treatment on a MAb process. R. S. Rosenthal

9:00 —195. Viral clearance strategies using improved membrane adsorbers. W. T. Riordan, M. R. Etzel


9:40 —196. Standardization of purification process development for mAbs & Fc-fusion proteins. R. Godavarti, G. Bolton, J. Booth, P. R. Brown, J. Coffman, C. Esenther, C. Gallo, T. Iskra, S. Sun, M. Switzer, S. A. Tobler, R. Wright, B. Kelley

10:00 —197. Characterization of a reversed phase chromatography step. X. Hu, A. Wang, Y. Lu, O. Kaltenbrunner

10:20 —198. Strategies for maximizing the clearance of impurities across an anion exchange flow through step in the context of an integrated antibody purification process. A. Kundu, A. Miller, J. Einerwold, M. Xia, R. Duffy, W. Zhou

10:40 —199. Platform ultrafiltration process development: Charge and conductivity effects on ultrafiltration performance of monoclonal antibodies. A. S. Len, R. van Reis

Section DUnknown Site -- Unknown RoomUpstream Processing: Advances in Cell Culture Process Development

S. T. Sharfstein, I. Blumentals, and G. Maheshwari, Organizers

8:00 — Introductory Remarks.

8:05 —200. High-producing CHO-GS and NS0-GS cell line generation and characterization using Cell Xpress(TM) technology. N. Lin, G. Richardson, L. Davis, K. Lacy, M. Gray, J. Cresswell, A. Dennett, M. Gerber, M. Caple, K. Kayser

8:25 —201. Developments in using the ACE System to generate high MAb expressing, stable CHO cell lines. M. L. Kennard

8:45 —202. Proteomic and genomic studies of mammalian cell physiology to optimize production of therapeutic and diagnostic proteins. D. Shen, T. R. Kiehl, S. T. Sharfstein


9:25 —203. Impact of scale and bioreactor type on cell growth, productivity, product quality, and global gene expression. M. A. Hanson, J. Vallejos, X. Ge, K. Brorson, A. R. Moreira, G. Rao

9:45 —204. Sodium butyrate stimulates mAb over-expression in CHO cells by improving gene accessibility. Z. Jiang, S. T. Sharfstein

10:05 —205. Improved analytical technique for high throughput analysis of glycoforms. M. P. Gillmeister, N. Tomiya, S. J. Jacobia, Y. C. Lee, S. F. Gorfien, M. J. Betenbaugh


Section EUnknown Site -- Unknown RoomElmer Gaden Award Lecture

W. Zhou, Organizer11:30 —206. Directing the assembly of multifunctional biomolecular architectures. J. S. Dordick

WEDNESDAY AFTERNOON

Section AUnknown Site -- Unknown RoomBIOT Young Investigator Award Lecture

W. Zhou, Organizer2:00 —207. Building proteins with new therapeutic functions from peptide modules. P. S. Daugherty

Section BUnknown Site -- Unknown RoomEmerging Technologies: Systems BiologyCosponsored with BIOHWC. Chan and C. Cho, Organizers3:00 —208. Dissection of the HIV promoter and possible contribution of NFκB and Sp1 to phenotypic bifurcation and latency. J. C. Burnett, A. P. Arkin, D. V. Schaffer

3:20 —209. A new kinetic model reveals the synergistic effect of E-, P- and A-sites on +1 ribosomal frameshifting. P -Y. Liao, P. Gupta, A. N. Petrov, J. D. Dinman, K. H. Lee

3:40 —210. Global assessment of protein turnover rates in Streptomyces coelicolor using a multitagging proteomic strategy. K. P. Jayapal, S. Sui, R. J. Philp, Y -J. Kok, M. G. Yap, T. J. Griffin, W -S. Hu


4:20 —211. Universal Sequence Tag ARray (U-STAR) platform: A universal platform for the quantitative analysis of transcriptomes. A. P. So, C. A. Haynes

4:40 —212. Proteome-wide identification of member-specific natural substrate repertoire of caspases and other proteases. R. Liu, C. A. Valencia

5:00 —213. Cellular scale qualitative shotgun proteomic analysis of Nostoc sp. PCC 7120 isolated heterocysts and filaments under nitrogen deficient conditions using 8-plex isobaric peptide tags. S. Y. Ow, K. Stensjö, P. C. Wright, P. Lindblad

Section CUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Targeted Delivery of Proteins & Nucleotides

C. Meares and W. Liu, Organizers3:00 — Introductory Remarks.

3:05 —214. N-ethoxybenzylimidazoles (NEBIs) as potential pH-sensitive linkers for drug delivery. A. Luong, S. D. Kong, G. Manorek, S. B. Howell, J. Yang

3:25 —215. Selective antitumor aptamer-photodynamic agent conjugates as a tool in photochemo therapy. P. Mallikaratchy, W. Tan

3:45 —216. Synthesis, phototoxicity and metabolic stability of porphyrins conjugated to bifunctional peptide sequences. M. G. H. Vicente, M. Sibrian-Vazquez


4:20 —217. Optical antisense imaging of tumored mice using a fluorescent DNA duplex. X. Liu, Y. Wang, K. Nakamura, G. Liu, S. Dou, A. Kubo, M. Rusckowski, D. J. Hnatowich

4:40 —218. Specific cellular delivery of quantum dot-peptide and quantum dot-polymer nanoassemblies. J. B. Delehanty, D. Farrell, I. L. Medintz, H. Mattoussi

5:00 —219. Transencapsidation of Semliki Forest virus RNA with tobacco mosaic virus coat protein: Evaluation of antigen expression, antigen delivery and vaccine efficiacy. A. A. McCormick, T. Corbo, J. Bernales, J. A. Lindbo, K. E. Palmer, M. L. Smith

Section DUnknown Site -- Unknown RoomCommercialization of Biologics: Characterization and Improvement of Platforms to Aid Commercialization of Biologics

S. Gadam and F. Olsen, Organizers3:00 —220. Development and implementation of a robust chemically-defined and high-yielding GS-CHO platform mAb production process. S. A. Casnocha

3:20 —221. Use of mathematical modeling to enable effective implementation of platform aeration strategies in process scale-up and transfer. G. Nyberg, F. Jimenez, F. Olsen, G. Grampp

3:40 —222. Robustness screening to enable cell culture process commercialization. A. M. Rusiniak, T. Wang, G. Miroquesada


4:20 —223. Process improvement through media development. W. Wenge

4:40 —224. Rapid development of mammalian cell production process of clinical material for proof of principle of novel therapeutic molecules. J. Zhu, Y. Xie, V. V. Vyas, B. Keseling, J. Roach, G. Mitra, S. P. Creekmore

5:00 —225. Rapid upstream commercial development of an Escherichia coli.-based process for producing therapeutic proteins in inclusion bodies. H. Lin, I. Liu, H. Barkhordarian, J. Lull, X. Yang

Section EUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Protein Aggregation

C. J. Roberts and A. Grillo, Organizers3:00 —226. Biophysical characterization of the amylin-derived peptide pramlintide. A. Nonoyama, J. S. Laurence, L. Garriques, H. Qi, T. Le, C. R. Middaugh

3:20 —227. Silicone oil contamination of therapeutic protein formulations: Surfactant and protein effects. J. Gabrielson, D. G. Bates, B. M. Williams, J -B. Hamel, J. F. Carpenter, T. W. Randolph

3:40 —228. Self-interaction chromatography applied to the rapid development of formulation for therapeutic proteins. H. Kornmann, N. Collet, G. Baer


4:20 —229. Stability of a heterogeneous IgG1 antibody with atypical glycosylation: A comparison between individual and mixtures of its glycoforms. G. C. Chu, S. B. Coulibaly, H. K. Khor

4:40 —230. Discrepancies in the phase behaviors of therapeutic antibodies. B. Salinas, S. Hasige, C. Allan, S. Bishop, A. Shah, J. F. Carpenter, T. W. Randolph

5:00 —231. Chemometric approach toward protein aggregation propensity. M. C. Manning, C. M. Van Pelt, R. R. Meglen, F. J. Stevens

Symposium in Honor of Perry FreySponsored by BIOL, Cosponsored with BIOT, MEDI, ORGN, BTEC, and BIOHW

WEDNESDAY EVENING

Section AUnknown Site -- Unknown RoomPoster Session

Upstream Processing

Y. Tang, S. Ozturk, and S. Subramanian, Organizers5:30 - 7:30

232. Role of astroglial fatty acid metabolism in the pathogenesis of Alzheimer's disease: A metabolic flux analysis (MFA) study. S. Patil, C. Chan

233. Suppression of PKR activity mediates the apoptosis induced by palmitic acid and TNF-α in HepG2 cells. X. Yang, C. Chan

234. Studies on novel optimization parameters for production of protease using response

surface methodology. A. I. R. Chaduvula, K. K. Nalla, M. Y. Nikku, S. B. Imandi, S. K. Karanam, I. Yedluri

235. Evaluating biopharmaceutical economics and capacity with process modeling and simulation. A. Koulouris, C. Siletti, D. Petrides

236. Monitoring the expression of biosynthetic pathway genes in Catharanthus roseus hairy root cultures under optimum elicitation conditions. S. Goklany, R. H. Loring, C. W. Lee-Parsons

237. Analysis of suspended and biofilm atrazine degrading cells. N. K. Biglione, V. G. Rodgers, T. L. Peeples

238. Biosynthesis and application of colorants from fungi. F. Alihosseini, G. Sun

239. The evolution of a disposable stirred tank bioreactor at Centocor: From concept to GMP implementation. W. Christian, R. Bhatia, N. Richardson, S. Ozturk

240. Biotransformation of steroids by Exophiala jeanselmei var. lecanii corni. K. P. McCook, P. B. Reese

241. Multiplex transcript analysis for identifying high-producing cell lines. C. J. Lee, G. Seth, J. Tsukuda, R. W. Hamilton

242. ReBiT, a database for enzymatic pathway design. C. H. Martin, D. Prather, K. Jones Prather

243. Construction of ptb disrupted mutant for characterization of its function in Clostridium tyrobutyricum fermentation. Y. Zhang, S -T. Yang

244. Biotransformation of steroids by Thielaviopsis paradoxa. F. A. Russell, P. B. Reese

245. Continuous perfusion process in a disposable stirred-tank bioreactor: A case study. B. Chiang, N. Richardson, H. Haddock, C. Wood, R. Bhatia, S. Ozturk

246. Screening of strains for bioconversion from DL-ATC to L-cysteine. T. Dong, Z. Lin, Y. Huang

247. Development of generic feed media for protein production in contract manufacturing. G. Wang

248. Enhancement of mass transfer in a 250 L bioreactor by sparger design modification. C. Wood, H. Haddock, B. Cottee, S. Ozturk

249. Fed-batch process development: A platform-based approach. K. Stoeber, C. Ranucci, N. Villani

250. Fluid mixing practice for bioreactor scale-up and scale-down. R. D. LaRoche

251. Host system choice challenges bioprocessing of plant-derived therapeutics. L. R. Wilken, S. Woodard, G. Barros, S. White, Z. L. Nikolov

252. Implementing an automated reactor sampling system for monitoring cell culture bioreactors. L. Speciner, G. Barringer, J. Perez, C. Grimaldi, R. Reineke, A. Arroyo

253. Physiological characterization of Saccharopolyspora erythraea deletion strains: Global and pathway specific regulators. I. A. Akinrinsola

254. Production of Galacto-oligosaccharides from whey lactose by using two-step Plug-Flow Reactor with immobilized enzyme β-Galactosidase from Aspergillus oryzae and Bacillus circulans and posterior chromatographic separation. J. I. Sanz Valero, S -T. Yang

255. Rapid enhancement of antibody production through DOE-based hydrolysate supplementation of an optimized base medium. C. Hunt, K. Hammett, T. Hill, T. Oshunwusi, S. Holdread, Y. Bai, J. W. Brooks

256. Simultaneous determination of multiple components in CHO cell culture using raman spectroscopy. S. Varma, S. Sakhamuri, M. Hausladen, Z. Li, S. S. Lee

257. Strategies for implementation of a new bioprocess container in commercial biologics manufacturing. R. Marcklinger, C. Williamson, J. Gates, A. A. Shukla, S. Lee

258. Toward reproducible and optimal operation of mammalian cell cultures: A model based approach for fault detection and isolation. S. Karra, M. N. Karim

259. Using CFD to model gas transfer in bioreactors. W. J. Kelly, J. Anderson

260. Improvement of the bleachability and brightness of wheat straw chemomechanical pulp with a pretreatment of xylanase from Trichoderma reesei. F. Hong

261. Using MAb enhancing compounds to investigate the MAb production pathway. T. D. Rau, R. R. Balcarcel

262. Analysis of gene dosage effects for metabolic engineering in Escherichia coli. N. Negron Rodriguez, K. Schumacher, K. Jones Prather

263. Analysis of substrate metabolism for an immobilized CYP2C9 construct: A potential bioreactor application. L. Wollenberg, J. L. Kabulski, T. S. Tracy, D. Lederman, P. M. Gannett

264. Isolation and identification of acetic acid bacteria from Shanxi superior mature vinegar in China. N. Yang, L. Hao

265. Effect of β-Mercaptoethanol on the fed-batch performance of GS-NS0 cells. Z. Naji, S. Fries, M. Chartrain, C. Seamans

266. Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum. M. Sasaki, H. Kawaguchi, S. Okino, T. Jyojima, M. Inui, H. Yukawa

267. Novel large-scale production of genetically engineered elastin-like-biopolymer and endotoxin-binding peptide fusion for neutralization of endotoxin. W. Jia, Y. Lei

268. Establishment of a WaveTM bioreactor scale-down model for process development and characterization studies. S. F. Khattak, S. Sakhamuri, Z. Li, S. Lee

269. Expression of recombinant human erythropoietin having more complex N-glycans in Drosophila S2 cells through hexosaminidase inhibition. Y. K. Kim, K. R. Kim, Y. H. Kim, H. J. Cha

270. Inhibiting the apoptosis pathway using MDM2 in mammalian cell cultures. N. Arden, B. S. Majors, S -H. Ahn, G. A. Oyler, M. J. Betenbaugh

271. Monitoring of glucose in cell culture by glucose binding protein (GBP) combined with ultraslow microdialysis. X. Ge, M. A. Hanson, G. Rao, L. Tolosa

272. The impact of residual air in bioreactor sterilization. W. J. Kelly, S. Hannon, M. Paddock, T. B. Vickroy

273. Optimization of monoclonal antibody production using process simulation and scheduling tools. V. Papavasileiou, D. Petrides, C. Siletti

274. Proteomic investigation of elicited Eschscholzia californica cultures producing enhanced levels of benzophenanthridine alkaloids. J. T. Oldham, M. Hincapie, T. Rejtar, J. G. Dy, C. W. Lee-Parsons

275. Rational design of main-chain elongated substrates acceptable by the E.coli ribosome. K. Mizusawa, S. Sando, Y. Aoyama

276. Using a global model of protein production with substrate competition to explain the complex mRNA vs. protein relationship arising from genetic perturbations. R. S. Kuczenski, K. H. Lee

277. Recombinant baculovirus-based multiple protein expression platform for Drosophila S2 cell culture. K. R. Kim, Y. K. Kim, H. J. Cha

278. Systematic approach to troubleshooting in support of a late stage antibody manufacturing campaign. L. Ly, M. Wyckoff, C. Lee, V. Tsang, H. Yusuf-Makagiansar

279. Reduction in high mannose glycoforms in mammalian cell culture through media optimization. N. Le, B. Pendleton

280. Development of novel sourdoughs with the use of different cereal flours. H. Hernandez-Sanchez, I. Nava-Arenas

281. Production of 14-oxo-cis-11-eicosenoic acid from lesquerolic acid by genetically variable Sphingobacterium multivorum strains. T. M. Kuo, A. Rooney, T. A. Isbell

282. Production of D-lactic acid by the RITE bioprocess using genetically engineered Corynebacterium glutamicum. S. Okino, M. Suda, M. Inui, H. Yukawa, Y. Tsuchida

283. Effects of redox buffers on the folding of disulfide containing proteins. W. J. Lees, M. Gurbhele, F. Kibria

284. Bridging timescales between atomistic simulation and experiments with master equation models of protein folding and dynamics. J. D. Chodera, N. Singhal, I. Haque, W. C. Swope, J. W. Pitera, K. A. Dill, V. S. Pande

285. A simple and generic immunoplatform for detecting histidine tagged proteins based on biofabrication. H -C. Wu, X. Shi, A. T. Lewandowski, C -Y. Tsao, R. Fernandes, C -W. Hung, P. DeShong, E. Kobatake, G. F. Payne, W. E. Bentley

Section BUnknown Site -- Unknown RoomPoster Session

Downstream Processing


286. Simulation of an industrial ion exchange step: The use of simulation for robustness analysis. T. B. Hansen, L. Sejergaard, E. Hansen, S. Kidal

287. Simulation of an industrial ion exchange step: The use of simulation to yield a constant output with a variable input to a process step. S. Kidal, E. Hansen, L. Sejergaard, T. B. Hansen

288. Strategies for integrating spectral data from raw materials to control biopharmaceutical manufacture. M. Lanan

289. Characterization of peak broadening during size-exclusion chromatography of IgG2 antibodies. A. Leuras, E. Babcock, T. Li, R. Rajan

290. Cell culture conditioning to improve harvest performance and impurity removal. M. Westoby, A. Koswara, R. Haverstock

291. Column simulations of chemically selective displacement systems. A. S. Freed, C. J. Morrison, S. M. Cramer

292. Interrelating protein precipitation and hydrophobic interaction chromatography. B. K. Nfor, K. Wiedhaup, T. Ahamed, L. A. M. van der Wielen, G. W. K. van Dedem, E. J. A. X. van de Sandt, M. H. M. Eppink, M. Ottens

293. Purification of recombinant collagen from transgenic corn seed. C. Zhang, J. Baez, C. E. Glatz

294. Activation of the IRF-3 transcription factor: The mechanisms of phosphorylation-induced IRF-3 dimerization and DNA binding. A. I. Dragan, V. Hargreaves, E. N. Makeyeva, P. L. Privalov

295. Chromatographic HTS and simulation for improved process development and optimization. M. Bensch, J. Nielsen, J. Krarup, T. B. Hansen, S. Kidal, L. Sejergaard

296. Impact of process parameters on the performance of Normal Flow Parvovirus (NFP) filters in an antibody purification process. J. Einerwold, J. Jorgensen, B. Petersen, A. Kundu

297. Stability of the bZIP homo- and heterodimers and energetics of their interactions with DNA. R. Carrillo, A. I. Dragan, P. L. Privalov

298. Strategies for optimizing upstream clarification of cell culture fluids. B. Raghunath, B. Cacace, D. Yavorsky

299. Accelerated physico-chemical characterization of complex protein mixtures for optimized ion-exchange chromatographic separation. A. Berrill, S. Ho, N. Titchener-Hooker, D. G. Bracewell

300. High throughput synthesis and screening for membrane filtration. M. Zhou, J. E. Kilduff, D. G. Anderson, R. S. Langer, G. Belfort

301. Investigation of chemically selective displacers using robotic high throughput screening, SPR, NMR and MD simulations. C. J. Morrison, S. A. McCallum, R. Godawat, J. Moore, S. Garde, S. M. Cramer

302. Platform technology for developing purification processes. M. H. M. Eppink, R. Schreurs II, A. Gijsen, B. Kamps, K. Verhoeven

303. Rapid optimization of Protein A chromatography using a high-throughput format. A. Gronberg, F. Lindstrom, T. Bjorkman, G. Rodrigo

304. Streamline hydrophobic interaction chromatography resin screening for protein purification process development. J. Ma, P. Jorjorian, C. Mason, R. Bates

305. Bench scale cleaning characterization of drug products in a fill-finish facility. N. Rathore, N. Bhasin, P. Kolhe, R. Law, A. Abdul-Fattah, L. Li, W. Ji, K. Murphy

306. Regulatory strategy for changing a chromatography resin in a licensed process by

the use of a comparability protocol. M. J. Rubino, P. R. DeVoe

307. Chromatographic HTS and simulation for improved process development and optimization. M. Bensch, J. Nielsen, J. Krarup, T. B. Hansen, S. Kidal, A. Stabe, J. Hubbuch, E. Hansen, L. Sejergaard

308. Engineering the adsorption of plasmid DNA. W. J. Kelly, P. Butts

309. AKTA Crossflow for characterization of an ultrafiltration and diafiltration step. C. W. Richey Jr.

310. Development of lab scale systems to evaluate micromixing in biological separations. J. Girard, M. P. Watson

311. ELISA based selection of elution buffer for IgG-affinity chromatography. R. Varakala, R. R. Beitle, K. Clark, T. W. Smith

312. Enantioselective hydrolysis of epoxides in organic solvents by Beauveria bassiana. E. G. Ankudey, T. L. Peeples

313. Risk mitigation and optimization strategies for viral filtration. L. Flansburg, R. Forng, S. Lee, A. A. Shukla

314. Robust microscale method for yeast cell disruption in the purification of intracellular proteins. M. D. Wenger, P. DePhillips, D. G. Bracewell

315. Anomalous peak splitting of a human IgG2 mAb on HPLCSEC caused by charge variants. J. Martin, M. Dupuis, J. Kwong, G. Waszak

316. Buffer distribution capacity analysis at the pilot scale. A. Caparoni, J. B. Fletcher, J. Sinclair

317. Characterization of a new IgG binder adsorbent. P. Adielsson, S. Eriksson, F. Alamravesh, H. Wlad, J. Färenmark

318. Characterization of cation exchange adsorbents for monoclonal antibody purification. D. Roth, C. Daniels, B. To

319. Coimmobilization of pyranose 2-oxidase, laccase, and catalase on solid supports for enhanced production of 2-keto-aldoses. R. Ludwig, P. Sukyai, T. Resic, D. Haltrich

320. Development of a high throughput purification method for analysis of cell culture samples. S. Koob, N. Barron, J. H. Chou, M. Sinacore

321. Development of a high-capacity downstream process for an NS0-derived human IgG2 based on 11-12 day fed batch fermentations with defined feed media. G. Waszak, E. Yu, M. Dupuis, J. Martin

322. Development of Biacore application for influenza virus titer determination. C. Estmer Nilsson, M. Bängtsson, A. Larsson, R. Lemmens

323. Monte-Carlo simulations of dye-labeled proteins diffusing in nanopores. J. Hohlbein, M. Steinhart, E. Hinze, C. Schiene-Fischer, C. G. Hübner, U. Gösele

324. ELISA for Quantifying trace amount of an alkaline stabilized protein A in high concentration of mAbs. T. Bjorkman

325. High resolution anion exchange chromatography purification of oligonucleotides. J. K. O'Donnell, E. Gershenow, B. Kempf, S. Nakatani

326. Enhancing the thermostability of β-mannanase by addition of sugars, polylols and their combinations. W. Wu, Z. Liu, W. Qi, Z. He

327. Evaluation of protein A chromatography media for capture of monoclonal antibodies. B. R. Nielsen, J. S. Nielsen

328. Extraction of intracellular oxalate decarboxylase from the white-rot wood decay fungus Trametes versicolor. C. X. Zhu, F. Hong

329. Impact of lot-to-lot variability of cation exchange resin on process performance. J. Wahome, A. Berhe, J. Jorgensen, J. Einerwold, W. Zhou, A. Kundu

330. Impact of process parameters on the performance of Normal Flow Parvovirus (NFP) filters in an antibody purification process. J. Einerwold, J. Jorgensen, B. Petersen, A. Kundu

331. Isolation, identification, and characterization of heavy chain clips and an expressed intron in a CHO-produced mAb. M. Dupuis, J. Finnessy, J. Leone, T. Carter, J. Martin

332. Kinetics and thermodynamics of thermal inactivation and the thermostable mechanism of protective additives for beta-mannanase. Z. Liu, W. Wu, W. Qi, Z. He

333. Libraries of multimodal chromatographic ion exchanger for the discovery of new selectivities and as a tool for HTPD. I. Blomqvist, P. Adielsson, J. Färenmark, J -L. Maloisel

334. Mechanisms of protein A leaching. C. Victa, J. N. Franklin, P. J. McDonald, R. Fahrner

335. Novel denitrification process of electrochemical biocatalyst and its stability. J. S. Cho, J. Y. Park, Y. T. Park, Y. J. Yoo

336. Preparation and characterization of alcohol dehydrogenase-containing liposomes for improving its quaternary structure. M. Yoshimoto, M. Sato, K. Nakao

337. Process-specific range finding of chromatography resin lots used in the purification

process of a recombinant product. L. Lee, J. Suchecki, K. Apgar, D. Abraham

338. Production of biodiesel by transesterification with new supported solid state alkali catalyst CaO/NaY. H. Wu, Y. Zhou, J. Zhang, J. Liu

339. Successful scale-up of pH elution on cation-exchange column for the purification of monoclonal antibodies. F. Zaman, J. Glynn, N. Ramasubramanyan, P. Mensah

340. Use of analytical ultracentrifugation with a fluorescence detector to characterize high protein concentration formulations. Z. W. Kwong, J. Moore, J. Liu, Y. Shen, Q. Zhu, T. Kamerzell, T. M. Laue, S. J. Shire

Section CUnknown Site -- Unknown RoomPoster Session

Biophysical & Biomolecular Engineering


341. A high-throughput genetic selection for protein folding and solubility in the bacterial periplasm. T. Mansell, M. P. DeLisa

342. Creation of a fluorescent protein biosensor. E. Shusta, T. Pavoor

343. Designing AraC effector specificity through modeling and dual selection. P. C. Cirino, S -Y. Tang, H. Fazelinia, C. D. Maranas

344. Gankyrin: A general model for Ankyrin Repeat (AR) protein studies. A. Mahajan, Y. Guo, M -D. Tsai, J. Li

345. High yields of complex proteins with site-specific posttranslational modification using cell-free protein synthesis. A. R. Goerke, J. J. Wuu, W. Ebina, J. R. Swartz

346. Mathematical model of protein secretion incorporating pore complex sequestration by aggregated protein. R. S. Kuczenski, P. Gupta, K. H. Lee

347. New design strategies for FRET-based sensor proteins. M. Merkx, E. M. W. M. van Dongen, T. H. Evers, J. L. Vinkenborg, L. W. J. Klomp, E. W. Meijer

350. Protein engineering of gene switches. H. Zhao, K. Chockalingam, M. McLachlan

351. Site-specific PEGylation of interferon–beta by Cu(I)–catalyzed cycloaddition. N. W. Nairn, T. J. Graddis, A. Wang, K. Shanebeck, K. Grabstein

352. Stability of the bZIP homo- and heterodimers and energetics of their interactions with DNA. A. I. Dragan

353. Structural stability and reactivity of bovine liver catalase modulated with liposomes. M. Yoshimoto, H. Sakamoto, K. Nakao

354. Resolution of disulfide “scrambling” through engineering of a modified form of the Nogo Receptor (NgR1) Fc fusion protein. P. H. Weinreb

355. Dendritic molecular transporters as highly targeted, controlled intracellullar delivery vectors. B. L. Evans III, K. Huang, M. Ikizler, P. F. Wright, E. Harth

356. Directed evolution of targeted cell penetrating peptides for trans-BBB delivery. S. Gao, M. Simon, B. Morrison III, S. Banta

357. Hemoglobin-drug conjugates for targeted delivery of nucleoside drugs. G. Adamson, D. N. Bell

358. Ketalized polyethylenimine for tunable delivery of plasmid DNA and siRNA to intracellular targets. M. S. Shim, Y. J. Kwon

359. Polyelectrolyte multilayer films as platforms for efficient siRNA delivery. S. Kidambi, S. Mehrotra, J. A. Gredell, S. P. Walton, I. Lee, C. Chan

360. Targeting mRNAs with a radiolabeled herceptin/tat/antisense MORF streptavidin nanoparticle. X. Liu, Y. Wang, K. Nakamura, A. Kubo, D. J. Hnatowich

361. The influence of different antitumor antibodies on anticancer effector accumulation in solid tumors by MORF pretargeting. G. Liu, S. Dou, M. Rusckowski, D. J. Hnatowich

362. Two novel anti-TAG-72 cancer biomarkers identified by phage display. L. Chen, M. Rusckowski, D. J. Hnatowich

363. Characterizing protein structure in amorphous solids using hydrogen/deuterium exchange with mass spectrometry. Y. Li, T. D. Williams, R. L. Schowen, E. M. Topp

364. A highly efficient selection strategy for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism. D. Waraho, M. P. DeLisa

365. Effects of water-miscible ionic liquids on the catalysis of horseradish peroxidase. K. Ryu, E. Hong

366. Formulation and characterization of components in vaccines. J. D. Chesko, J. Kazzaz, P. Malyala, D. O'Hagan, K. Patton, M. Singh

367. Amplification bias in competitive templates is offset through the use of proofreading DNA polymerases: Application to the amplification of SAGE ditags. A. P.

So, C. A. Haynes

368. Dual fluorescence reporter system for studying +1 programmed ribosomal frameshifting in Escherichia coli. P -Y. Liao, K. H. Lee

369. Stabilizing protein conformations in solution with osmolytes and cosolvents. C. H. Schein

370. Highly active enzyme formulations for use in nonaqueous media. A. L. Serdakowski, J. P. Lindsay, J. S. Dordick

371. Bioactive protein-based hydrogels for functional bioelectrode construction. I. Wheeldon, S. Calabrese Barton, S. Banta

372. Determining antibody stability: Creation of defined interfacial effects within a high shear environment. J. G. Biddlecombe, D. G. Bracewell, B. C. Fish, S. Uddin

373. Development and validation of a novel antioxidant capacity assay using sodA::gfp as a living sensor. W. A. Lea, L. Tolosa, G. Rao

374. Improved solubility and stability of cytochrome c in hydrated ionic liquids. K. Fujita, H. Ohno, D. R. MacFarlane, M. Forsyth

375. Surprising results of Pseudomonas aeruginosa heme oxygenase (pa-HO) ortho-19F phenylalanine labeling. A. Wang, M. Rivera

376. Detection of biomolecules by magnetic reporting of labeled protein nanorods. T. P. Harrah, J. Xie, J. I. Oh, M. J. Naughton, S. Sun, R. Guertin, E. B. Goldberg

377. Detection of s-phase cell cycle progression using ethynyl-2'-deoxyuridine incorporation with click chemistry. S. B. Buck, S. T. Clarke, J. A. Bradford, K. Gee, B. Agnew, A. Salic

378. Structure and dynamics of proteins from designed combinatorial libraries. A. Go, S. Kim, J. Baum, M. H. Hecht

379. Quantifying the effects of electrostatic forces and microstructure of protein by the change in Gibbs free energy of binding in IMAC. L. Pathange, D. Bevan, C. Zhang

380. Engineering of self-assembled vault protein nanocapsules. M. Yu, L. E. Goldsmith, V. A. Kickhoefer, L. H. Rome, H. G. Monbouquette

381. Enzyme characterization at the nano-bio interface. J. L. Kabulski, L. Wollenberg, M. Yang, N. N. Wu, T. S. Tracy, D. Lederman, P. M. Gannett

382. Fibrinogen self-assembled fibrils on Clay monolayer templates. J. Koo, T. Koga, D. Galanakis, M. Rafailovich, J. Sokolov

383. Instantaneous distribution of cellular tractions during fibroblast migration. Z. Pan, Y. Liu, K. Ghosh, T. Nakamura, R. A. F. Clark, M. Rafailovich

384. Integrated platforms for engineered cell-cell communication via cell-directed assembly. E. C. Carnes, C. E. Ashley, D. M. Lopez, C. M. Douthit, D. R. Dunphy, C. J. Brinker

385. Nanobiobased electrochemical biosensors for cisplatin detection. J. E. Jett, N. N. Wu, H. Li, P. M. Gannett

386. Nanotube-assisted protein inactivation. A. Joshi, S. Punyani, T. Borca-Tasçiuc, R. S. Kane

387. One pot synthesis and biotinylation of CdS nanocrystals by incorporating intein-mediated protein splicing. U. L. Lao, S. H. Kang, A. Mulchandani, W. Chen

388. Phytochelatin-mediated synthesis of cadmium chalcogenide semiconductor nanocrystals. S. H. Kang, A. Mulchandani, W. Chen

389. Proteomic characterization of E. coli metal binding proteins. R. Varakala, R. Haley, R. R. Beitle, M. M. Ataai

390. Screening the transcriptional machinery for potent artificial transcriptional activators. C. Y. Majmudar, A. K. Mapp

391. Single molecule analysis of DNA/protein interactions: The nanopore shift assay. M. Wanunu, D. Cohen-Karmi, Y. Yu, Z. Weng, A. Meller

392. Targeting gold probes to vault nanocapsule interiors using mINT “shuttles”. L. E. Goldsmith, L. H. Rome, H. G. Monbouquette

393. The mechanism of arginine interaction with proteins. K. Tsumoto, D. Ejima, T. Arakawa

394. Ultrafast sensing platform based on surface plasmon-coupled emission. D. S. Smith, Y. Kostov, R. Badugu, G. Rao

395. A new strategy for acylation of tRNA with unnatural substrates. H. Masu, M. Hayami, S. Sando, Y. Aoyama

396. Direct measurement of binding interactions among split inteins. M. Sorci, B. Dassa, G. Anand, S. Pietrokovski, G. Belfort

397. Development of a novel method for direct nanopatterning of bacteriophage using dip-pen nanolithography. S. F. Filocamo, C -Y. Chiang, A. M. Belcher, C. M. Mello

398. Facile and rapid direct gold surface immobilization with controlled orientation for diverse carbohydrate types. J. H. Seo, D. G. Kang, Y. K. Kim, K. R. Kim, H. Y. Lee, H.

J. Cha

399. Impact of target mRNA structure on silencing efficiency and immune stimulation in RNA interference. J. A. Gredell, A. Berger, S. P. Walton

400. Influence of α-gliadin and poly(hydroxyethyl methacrylate-co-4-styrenesulfonic acid) concentrations on the structure of α-gliadin. L. Liang, J -C. Leroux, M. Subirade

401. Noncovalent immobilization of proteins on a solid surface by cucurbit[7]uril- ferrocenemethylammonium pair, a potential replacement of biotin-avidin pair. K. Kim, I. Hwang, K. Baek, M. Jung, Y. Kim, K. M. Park, D -W. Lee, N. Selvapalam

402. Research of BODIPY fluorescence dyes-NHS derivates with high biochemical activity. W. Dongchuan, P. Xiaojun, F. Jiangli

403. Sensing of nucleic acid sequence using unmodified DNA/RNA as a probe. A. Narita, S. Sando, Y. Aoyama

404. Sterilizable optical sensor for alcohol measurements. Y. Kostov, G. Rao

405. Tyrosinase immobilization on carbon nanoparticles. K. S. Min, J. Y. Park, J. S. Cho, Y. J. Yoo

Section DUnknown Site -- Unknown RoomPoster Session

Stem Cells & Emerging Technologies


406. Evaluation of UVC reactors using photochemically active fluorescent microspheres. A. Lewis

407. Deregulation of CAP dependent translation can play a role in the malignant transformation of eukaryotes. S. Nayak, J. K. Siddiqui, J. D. Varner

408. Identifying toxicity pathways through analysis of global transcriptional dynamics. Z. Li, S. Srivastava, C. Chan

409. Systems analysis of cellular development and apoptosis in taxus cell lines. Y -J. Yuan

410. Unsupervised classification and identification of physiologically relevant features by integrating ontology information and expression data using Bayesian regression mixture

models. S. Srivastava, R. Jin, C. Chan

411. Coculture of human embryonic stem cells with murine embryonic fibroblasts on microwell patterned substrates. A. Khademhosseini, L. Ferreira, J. Blumling, J. Yeh, J. M. Karp, J. Junji Fukuda, R. Langer

412. An imaging chamber for cultures of human islets of Langerhans. D. Moogk, S. Hanley, J. Ramunas, A. Blaylock, J. Skorepova, L. Rosenberg, E. Jervis

413. System to study colony-colony interactions in murine embryonic stem cells. S. Sampattavanich, J. Voldman

414. Effects of fiber alignment on the behavior of human dermal fibroblasts on the 3-D Poly (methyl methacrylate) (PMMA) electrospun scaffold. Y. Liu, Y. Ji, K. Ghosh, R. A. Clark, J. C. Sokolov, M. H. Rafailovich

415. Hill colonies, a putative endothelial progenitor cell derived population, are non-clonal and result from aggregation of multiple cell types as determined using long term imaging. C. S. Russell, A. Blaylock, J. Ramunas, L. Kelly, E. Clarke, C. G. Pereira, A. W. Wognum, T. Thomas, A. Eaves, E. Jervis

416. Long-term expansion of embryonic stem cells in serum-free media containing plant protein hydrolysates. N. Liu, S -T. Yang

417. Application of centrifugal force to enhance cell seeding in tissue engineering scaffold. R. Ng, X. Zhang, S -T. Yang

418. Biomechanical properties of tissue engineered cartilage. F. Horkay, D. C. Lin, I. Horkayne-Szakaly, E. K. Dimitriadis, C. Silva, P. J. Basser

419. Characterization and isolation of neurons differentiated from mouse ES cells by DRG-conditioned medium. A. Kitazawa, N. Shimizu

420. Culture under low oxygen conditions markedly enhances differentiation of murine embryonic stem cells into cardiomyocytes. J. R. Millman, D. E. Powers, J. P. M. Mattos Almeida, C. K. Colton

421. Engineering polymerized hemoglobins for use in transfusion medicine. A. F. Palmer

422. Enhanced albumin production by hepatic lineage cells derived from mouse embryonic stem cells. C -C. Hsiao, C -H. Yin, W. Chen, C -L. Chen, W -T. Wu

423. Efficient ethanol production from glucose and xylose mixture using growth-arrested Corynebacteria. S. Sakai, M. Sasaki, M. Suda, Y. Tsuchida, S. Okino, T. Jyojima, M. Inui, H. Yukawa

424. Evaluation of production of bioethanol and biodiesel from renewable resources

using process simulation tools. D. Tsangaris, C. Siletti, D. Petrides

425. Heterologous expression of Hydrogenovibrio marinus NiFe-hydrogenase in Escherichia coli for biohydrogen production. J. Kim, H. J. Cha

426. Lignin blockers to reduce costs of enzymatic hydrolysis of pretreated cellulose. C. E. Wyman, B. Yang, D. Willies

427. Preparation of microbial oil by fermentation for biodiesel production. Y. Mo, H. Liu, J. Zhang, X. Chen

428. Study on mild alkaline/oxidative pretreatment of bagasse. Z. Zhao, K. Cheng, J. Zhang, F. Gao

THURSDAY MORNING

Section AUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Protein Engineering

G. Makhatadze and R. S. Rajan, Organizers8:00 —429. Engineering and design of repeat proteins. L. Regan, A. L. Cortajarena, G. Haran

8:20 —430. Enzymatic activity from a designed artificial proteome. S. C. Patel, M. H. Hecht

8:40 —431. Computational procedure for transferring new binding sites into existing protein scaffolds. H. Fazelinia, P. C. Cirino, C. D. Maranas

9:00 —432. Dynamics in computational protein engineering. N. V. Dokholyan


9:40 —433. Protein engineering of extremophilic chaperones for molecular self-assembly. T. A. Whitehead, D. S. Clark

10:00 —434. Rational approaches to protein design and engineering. P. J. Reeder, C. Bystroff, J. S. Dordick

10:20 —435. Structure-based humanization of therapeutic antibodies. K. Hanf, Y. Li, K. Simon, L. L. Chen, M. Jarpe, E. Garber, F. Taylor, L. Silvian, J. Arndt, A. Lugovskoy

10:40 —436. Molecular strategy for designing robust proteins: Looking deeper at the surface. G. Makhatadze

Section BUnknown Site -- Unknown RoomDownstream Processing: Non-Chromatographic Separation Techniques: Improving Process Throughput

G. Vedantham and D. W. Wood, Organizers8:00 —437. Crystallization of therapeutic proteins and antibodies at Genentech: Purification and bulk storage applications. B. A. Bean, T. C. Matthews

8:20 —438. Detergent-mediated liberation of intracellular recombinant virus-like particles (VLPs) from S. cerevisiae homogenate. G. S. Kee, H. Pujar, N. Titchener-Hooker

8:40 —439. Elastin-like polypeptide-intein mediated protein purification. A. R. Gillies, D. W. Wood

9:00 —440. Large-scale antibody purification using non-chromatographic methods. S. Ramanan, R. Stenson


9:40 —441. Salt-dependent transmission of plasmid DNA through ultrafiltration membranes. D. R. Latulippe, A. Zydney

10:00 —442. Selective precipitation using polyelectrolytes: A novel approach to the purification of monoclonal antibodies. P. J. McDonald, J. Carter-Franklin, R. Fahrner

10:20 —443. Selective precipitation-assisted recovery of immunoglobulins from bovine serum. A. Venkiteshwaran, P. Heider, G. Belfort

10:40 —444. Can affinity-sinking become an alternative to affinity-chromatography? G. Patchornik

Section CUnknown Site -- Unknown RoomCommercialization of Biologics: Scale-up & Process Design for Commercial Facilities: Debottlenecking and Facility Fit

R. Kiss and N. Titchener-Hooker, Organizers8:00 —445. Biopharmaceutical portfolio management and capacity planning: A stochastic multiobjective optimization approach. E. D. George, S. Farid

8:20 —446. Gauging the technical, regulatory and financial implications of process changes. I. Hassan, M. Bulmer, J. More, S. Farid

8:40 —447. Industrial case studies of debottlenecking and future proofing through discrete event simulation. P. A. Sinclair

9:00 —448. Prototype software methodology for the rapid evaluation of biomanufacturing process options. S. Chhatre, R. Francis, K. O'Donovan, N. J. Titchener-Hooker, A. R. Newcombe, E. Keshavarz-Moore


9:40 —449. Facility fit, when should it happen? J. D. Harms, A. M. Tsai, A. S. Rathore

10:00 —450. Facility fit: Ensuring process performance comparability for commercial antibody processes when transferring to differently designed equipment. K. L. White, R. Garcia, K. Carswell, E. Ordonez, A. Bhartiya, B. Jones, T. Renshaw, R. Kiss

10:20 —451. Strategies for increasing downstream capacity. D. H. Peers, M. Ratna

10:40 —452. Can modular construction help to address key bottlenecks in the retrofit projects? T. Kimmel

Section DUnknown Site -- Unknown RoomUpstream Processing: Advances in Tissue Engineering

J. B. Leach and A. Coury, Organizers8:00 —453. Dual growth factor loaded PEGylated fibrin gels to control SMC phenotype from human MSC. C. T. Drinnan, L. J. Suggs

8:20 —454. A 3-D tissue model for high-throughput drug discovery. X. Zhang, R. Ng, S -T. Yang

8:40 —455. Intestinal epithelial cell response to crypt-like substrate topography. L. Wang, S. K. Murthy, R. L. Carrier

9:00 —456. Novel cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide. D. S. Hwang, Y. Gim, H. J. Cha


9:40 —457. Tissue-like self assemblance of hepatocytes in vitro by manipulation of collagen. Q. Meng, J. Dai, G. Zhang

10:00 —458. Anticancer drug test in tissue engineered perfusion microbioreactor array. Y. Wen, X. Zhang, S -T. Yang

10:20 —459. Targeted oxygen delivery to hepatic hollow fiber bioreactors via hemoglobin-based oxygen carriers. A. F. Palmer

10:40 —460. Novel systems for alginate-immobilized mammalian cell culture in bioreactors. C. A. Hoesli, K. Raghuram, M. Luu, J. Osborne, G. Korbutt, J. M. Piret

THURSDAY AFTERNOON

Section AUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Protein Engineering

G. Makhatadze and R. S. Rajan, Organizers2:00 —461. Beyond biology: Engineering new structure and function with computational protein design. J. G. Saven

2:20 —462. Directed evolution of conformational changes in peptides. M. Blenner, K. Chockalingam, S. Banta

2:40 —463. Engineering allosteric inteins for use as biosensors or in bioseparations. A. R. Gillies, D. W. Wood

3:00 —464. Insights into protein splicing and the engineering of inteins. B. Pereira, G. Belfort, S. Garde, V. Derbyshire, M. Belfort


3:40 —465. Picomolar affinity fibronectin antibody mimics by yeast display. B. Hackel, K. D. Wittrup

4:00 —466. Intracellular ribosome display for selection of synthetic antibodies in the bacterial cytoplasm. L. M. Contreras Martinez, M. P. DeLisa

4:20 —467. Structure-guided optimization of a therapeutic bispecific antibody. A. Lugovskoy, K. Hanf, B. Miller, S. Demarest, J. Michaelson, X. Wang, E. Garber, J. Browning, S. Glaser

4:40 —468. Engineering protein stability using polyethylene glycol conjugation: GCSF as a case study. R. S. Rajan, T. Li, C. Sloey, M. Aras, W. Sutherland, D. N. Brems

Section BUnknown Site -- Unknown RoomDownstream Processing: High Throughput Screening in Process Development

J. Coffman and G. Jagschies, Organizers

2:00 —469. Chromatographic HTS and simulation for improved process development and optimization. A. Staby, M. Bensch, J. Nielsen, J. C. Krarup, T. B. Hansen, S. Kidal, L. Sejergaard, E. Hansen, J. Hubbuch

2:20 —470. Development and application of an automated, small-volume chromatography system for resin and condition screening. M. Teeters, D. Bezila, P. Alred, A. Velayudhan

2:40 —471. Fast acquisition of bioseparation process development data from crude protein mixtures. T. Ahamed, B. K. Nfor, E. J. A. X. van de Sandt, M. H. M. Eppink, P. D. E. M. Verhaert, G. W. K. van Dedem, L. A. M. van der Wielen, M. Ottens


3:20 —472. High throughput process development: Determination of dynamic binding capacity using microtiter plates filled with chromatography resins. K. M. Lacki, T. Bergander, A. Grönberg, K. Öberg

3:40 —473. High throughput screening of protein A resin for purification process development. J. Booth, J. Coffman, B. Kelley

4:00 —474. High throughput screening in downstream processing – from solubility studies to packed bed chromatography systems. J. Hubbuch

Section CUnknown Site -- Unknown RoomDownstream Processing: Disposable Bioprocessing

D. D. Frey and S. Ramanan, Organizers2:00 —475. Regulatory considerations for using disposable technologies in bioprocessing. A. Moreira

2:20 —476. A comparison of conventional and controlled freezing methods. M. Olsen

2:40 —477. Disposable chromatography in antibody manufacturing: The good the bad and the ugly. U. Gottschalk


3:20 —478. Scalability of disposable Mixer system with a single-use levitating impeller. A. Terentiev

3:40 —479. Use of a disposable tangential flow filtration skid to manufacture monoclonal antibodies. H. Santhanam

4:00 —480. Viral clearance using disposable systems in Mab commercial downstream process. J. Zhou

Section DUnknown Site -- Unknown RoomBiophysical and Biomolecular Symposium: Protein Aggregation

T. Przybycien and M. Ricci, Organizers2:00 — Introductory Remarks.

2:10 —481. Does one folding defect lead to another? The importance of quaternary structure in transthyretin-mediated inhibition of beta-amyloid aggregation. L. Liu, J. Hou, R. M. Murphy

2:35 —482. Folding, unfolding, and fibril formation of human eye lens γC-crystallin. Y. Wang, J. King

3:00 —483. Molecular insights into self-perpetuating conformational changes of prions and amyloids. P. M. Tessier, S. Lindquist

3:25 —484. Folding, misfolding, and aggregation of polyglutamine peptides and proteins. M. Tobelmann, C. Lee, R. Walters, R. M. Murphy


4:10 —485. Refolding aggregation-prone GPCRs and G-protein α subunits from inclusion bodies expressed in Escherichia coli. E. C. McCusker, S. E. Bane, A. S. Robinson

4:35 —486. Nonnative protein polymers: Structure, morphology, and relation to nucleation and growth rates. W. F. Weiss IV, C. J. Roberts, A. M. Lenhoff

5:00 —487. Osmolyte controlled nucleation of insulin amyloid fibrillation. A. Nayak, C -C. Lee, G. J. McRae, G. Belfort

sabbatical leave — report of resultsmfey/gl/biotxcom/acs … · web viewinvestigation of protein...

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