safety in the microbiology lab
DESCRIPTION
Lab Safety: Everyone Is Responsible!. Safety In the Microbiology Lab. Rules and Symbols. Mr. Norazli Ghadin. Lesson objectives. To know the general rules in microbiology lab To know how to maintain aseptic condition To know how to discard microbiological waste - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/1.jpg)
Safety In the Microbiology LabRules and Symbols
Lab Safety: Everyone Is Responsible!
Mr. Norazli Ghadin
![Page 2: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/2.jpg)
Lesson objectives
• To know the general rules in microbiology lab
• To know how to maintain aseptic condition• To know how to discard microbiological
waste• To apply the rules when in the labs
![Page 3: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/3.jpg)
Importance of rules
• Ensure safety of operator (YOUR SELF!!!!!!)
• Safety of samples• Successful laboratory experimentation• Minimize hazards
![Page 4: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/4.jpg)
Routes of infection
• Oral
• Skin
• Conjunctiva
• Lungs
![Page 5: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/5.jpg)
General Safety Guidelines
• Be Responsible at All Times. • Follow all instructions carefully. • No horseplay, practical jokes, pranks,
etc.• Do not play with lab equipment until
instructed to do so.• Food, drink, smoke and gum are not
allowed in the microbiology lab .
Lab Safety: Everyone Is Responsible!
![Page 6: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/6.jpg)
General Safety Guidelines• Don’t lick labels. • Don’t drink from laboratory glassware• Don’t wander about the laboratory; cause:
– Accidents– Distract others– Promote contamination
• Operate centrifuges, homogenizer and shakers safely
• Immunize the laboratory workers
![Page 7: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/7.jpg)
General Safety Guidelines
• Keep the microbiology lab clean and organized.
• Notify supervisor immediately of any accidents or unsafe conditions in the microbiology lab !
• Wash your hands with soap and water after work.
Lab Safety: Everyone Is Responsible!
![Page 8: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/8.jpg)
General Safety Guidelines• Listen to or read instructions
carefully before attempting anything.
• Wear safety goggles to protect your eyes from chemicals, heated materials, or things that might be able to shatter.
• Notify your teacher if any spills occur.
![Page 9: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/9.jpg)
General Safety Guidelines
• Keep your hands away from your face.• Tie back long hair away from shoulders .• Wear glasses rather than contact lenses.• Never put into your mouth
– Pencils – Pens– fingers etc
• Clean up your lab area at the conclusion of the laboratory period.
![Page 10: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/10.jpg)
General Safety Guidelines• Roll up loose sleeves.• Button-up your Lab Coat• Lab coat thickening.• Know the location of the
fire extinguisher, Gas isolation switch, fire blanket, eyewash station, first aid kit & Exits.
• Keep your work area uncluttered.
![Page 11: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/11.jpg)
General Safety Guidelines
• Take to the lab station what is necessary, Left outside– Coats– Jackets– Bags and books not required
for the laboratory session.• Cover any open cuts on hands
and other exposed skin surfaces
![Page 12: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/12.jpg)
General Safety Guidelines
• Wear mask N95 4 mycobacterium. • Do not wear any jewellery.• Carry out procedures to minimize risks of :
– Spills– Splashes – Production of aerosols.
• No slides or cultures are to be taken from, or brought into the lab without permission.
![Page 13: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/13.jpg)
General Safety Guidelines
• Don’t use your Mobile.– Medical devices– Chemicals– With gloves– RF interference
• Do not attend calls with gloves.• Writing
– Without gloves– Working area
![Page 14: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/14.jpg)
General Safety Guidelines• Avoid wearing artificial nails• Keep natural nails <1/4 inch• Foot wear that completely
covers the foot is required• No Open shoes• No rings or hand accessories• Do not wear loose clothing that
could catch on fire• Do not open doors with glows
![Page 15: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/15.jpg)
PERSONAL PROTECTIVE EQUIPMENT and PROCEDURES • Gowns• Safety Glasses and Eye Protection• Gloves• Mask• Hand washing• High Risk Individuals /Antenatal
Considerations
![Page 16: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/16.jpg)
16
Hand washing
![Page 17: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/17.jpg)
Chemical Safety• Do not inhale the fumes.• Never pour water into a
concentrated acid.• Wash hands.• Wear goggles• Lab apron• Never taste any chemicals
(never taste anything).
![Page 18: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/18.jpg)
Electrical Safety• Lay electrical cords where no one can
trip on them or get caught in them.• hands dry before using electrical
equipment.• Never poke anything into electrical
outlets. • Unplug cords by pulling the plug and
not the cord.• Unplug all electrical equipment at the
end of the lab period.
![Page 19: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/19.jpg)
Heating Safety• Let burners and hotplates cool down
before touching. • Use tongs and/or protective gloves.• Never reach across an open flame. • Wait until the striker is in place before
you turn on the gas.• Heating a test tube, move it around
slowly. • Dry glassware should be heated.• Do not hold it in your hand • Never leave a burner or hotplate
unattended.
![Page 20: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/20.jpg)
Hypodermic syringes and needles
• Common causes of occupational infections.– Collection– Processing– 25%.
• Avoid quick & unnecessary hand movements.• Examine for chips and cracks, and needles for
barbs and plugs.• Use needle locking syringes.• Wear gloves.• Fill syringes carefully to minimize air bubbles
and frothing.
![Page 21: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/21.jpg)
Hypodermic syringes and needles• Expel air, liquid & bubbles vertically into a
disinfected cotton swab.• Don’t forcefully expel into an open vial for
mixing. • Mixing if the tip is held below the surface of the
fluid.• Don’t manipulate by hand.• Place directly into a puncture-resistant container.• Decontaminate before
– Disassembly– Reuse– Disposal.
![Page 22: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/22.jpg)
CLEANING & Waste Disposal
• Separate the non-infectious from the infectious waste.
• Dispose the infectious waste to– Minimizes the risk to both staff and students – Facilitates the recycling of reusable material.
![Page 23: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/23.jpg)
Sharps • “Yellow" sharps container at the back of each
laboratory.– Needles– scalpel blades– other sharp materials
• Always move the "sharps container" to your work place to dispose of such items.
• Do not wander around the laboratory carrying sharps.
![Page 24: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/24.jpg)
Biogram Buckets
• Containers of general purpose phenolic, hypochlorite (10 gm/L) laboratory disinfectant
• Used for disposal of small contaminated items– used swabs– capillary tubes– wet slides– Pipettes– inoculated reagent strips– glass culture tubes.
![Page 25: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/25.jpg)
Biogram Buckets• Disposing pipettes, place tip first into the
biogram • Not for Gram stains, non contaminated paper or
matches. • 1/40 dilution of the commercial product 18%
phenol equivalent. • Change weekly; or if grossly soiled.
![Page 26: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/26.jpg)
Biohazard Bin • Centre of each laboratory.• For the disposal of contaminated waste, e.g.
– Used culture plates – Contaminated paper towel.
• Not to be used sharps & Any non contaminated paper wastes.
• Do NOT use these bins for – Paper towel discarded after hand washing– Blotting paper discarded after blotting slides.
• autoclaved before disposal. • Left open to allow penetration of the steam
![Page 27: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/27.jpg)
Broken Glass
• When any glassware is broken, notify the instructor immediately for assistance with disposal. – Non contaminated can be disposed of in the
Billy cans– Contaminated should be placed into a stainless
steel Billy and autoclaved prior to disposal in the glass bin.
![Page 28: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/28.jpg)
Sink
• Don’t place any hazardous or infectious materials.
• Don’t dispose of any solid material.
![Page 29: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/29.jpg)
REPORTING OF INCIDENTS
• All accidents and major spills should be documented on an "incident report form" through the relevant Departmental Occupational Health and Safety Officer
• Copies of any documentation relating to an incident or safety hazard must be sent to both MSO and a copy should also be retained.
![Page 30: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/30.jpg)
First AidInjury: BurnsWhat To Do: Immediately flush with
cold water until burning sensation is lessened.
![Page 31: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/31.jpg)
First Aid
Injury: Cuts, bruises What To Do: • Do not touch without
gloves. • Pressing directly on minor
cuts. • Apply cold compress to
bruises to reduce swelling.
![Page 32: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/32.jpg)
First AidInjury: FaintingTo Do:
Provide fresh air and have the person recline so that their head is lower than the rest of their body.
![Page 33: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/33.jpg)
First AidInjury: EyesWhat To Do: • Flush eyes
immediately with plenty of water for several minutes.
• If a foreign object is lodged in the eye, do not allow the eye to be rubbed.
![Page 34: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/34.jpg)
First Aid
Injury: PoisoningWhat To Do: Find out what substance
was responsible for the poisoning and alert the
teacher immediately.
![Page 35: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/35.jpg)
First AidInjury: Spills on the skinWhat To Do: Flush with large
quantities of water. For acid spills, apply
baking soda solution. For base spills, apply vinegar or boric acid.
![Page 36: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/36.jpg)
First AidInjury: Electrical shockWhat To Do: • Shut off the current at the
source. • Remove wire with rubber
gloves. • Alert the teacher
immediately.
![Page 37: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/37.jpg)
What’s Wrong With This Picture?
![Page 38: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/38.jpg)
What’s Wrong With This Picture?
![Page 39: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/39.jpg)
What’s Wrong With This Picture?
![Page 40: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/40.jpg)
What’s Wrong With This Picture?
![Page 41: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/41.jpg)
When in doubt – ASK!!!• Do not carry out a
new or unfamiliar procedure until you have been fully trained & understand the precautions necessary for safe working
• DO NOT GUESS!!!!
![Page 42: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/42.jpg)
HAZARDS SYMBOLS
![Page 43: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/43.jpg)
Explosive• Chemical that will rapidly burn
and explode
![Page 44: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/44.jpg)
(Highly Flammable)
• ‘Flash point’ – temperature where vopour of a solution will burn if ignited.
• Solution with ‘Flash point’ < 32’c
![Page 45: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/45.jpg)
Oxidizing Substance
• Chemical that readily release Oxygen.
• Contoh: HNO3, KNO3, NaNO3, AgNO3
![Page 46: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/46.jpg)
Toxix• Harmful chemical• Cyanide and mercury
nitrate – nerve, kidneyte• ‘Iodine’ - lung and eye,
initiate ‘impetigo’• Formaldehyde (dalam
Larutan 40% dipanggil ‘formalin’) – an irritant
![Page 47: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/47.jpg)
(corrosive)
• All acid – burn tissue in contact.
• NaOH and KOH – irritate, vomit and diarhea
![Page 48: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/48.jpg)
(irretant & Harmful)
• Cylohexance, etanol, Methanol, Ethyl acetate, Butanol and Potassium dichromate - dermatitis.
• a dan b-Naphtol - skin irritant, damage kidney• Naphthylamine - anesthetic some drawbacks, irritation pada mucous
membrane
![Page 49: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/49.jpg)
Carcinogenic chemicals
• carcinogens, mutagens, teratogens, respiratory sensitizers and substances with target organ toxicity.
• Eg: • Ehtidium bromide,
benzene
![Page 50: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/50.jpg)
Environmentally hazards
• These substances damage or pollute the environment.
![Page 51: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/51.jpg)
Radiation hazards
• Ionizing radiation • E.g: Uranium 235
• Rhodium isotope
![Page 52: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/52.jpg)
Biohazards
• Living organisms that can cause infection
• bacteria, fungus, virus
![Page 53: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/53.jpg)
Reference• Food Standards Agency - Microbiological safety. html• Guidelines on Standard Operating Procedures for MICROBIOLOGY -
Safety in Laboratories. tm WHO• Microbiology Rules for Using Disinfectants eHow com. htmL• Microbiology Safety and Staff Induction Manual University of Tasmania -
Launceston Campus Version 2, February 2000• Mrs. Page Kennedy Middle School 2002-2003• Presented by: Leonard LaFazia
![Page 54: Safety In the Microbiology Lab](https://reader033.vdocument.in/reader033/viewer/2022061602/568163ea550346895dd55f1c/html5/thumbnails/54.jpg)