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Exploring the Diversity of Tropical Pumpkin in Costa Rica
Lizzie GillSustainable Agriculture and Food Systems
and International Affairs
IROPInternational Research Opportunities Program
Background
• Commercial agriculture• Vulnerable to change BIODIVERSITY = RESISTANCE
Tropical Pumpkin (Squash)• Cucurbita (squash, pumpkin, gourd)
– Cucurbita moschata (Butternut Squash)– Ayote sazón (seasoned squash) or calabaza
• Dr. Brent Loy• Economic importance• High carotenoid and vitamin A content
Costa Rica
Question
What is the level of phenotypic and genotypic diversity of Cucurbita moschata in Costa Rica?
Map diversity of winter squash/ayote sazón and record/inventory important characteristics
The Process
The Process
• Ferias (farmers market)
The Process
Alajuela
Fabio Baudrit Moreno Agriculture Experiment Station
Phenotypic Assessment(Appearance)
• Brix sweetness• Dry matter texture• Color nutrition status• Weight yield
Accession / muestra Fecha evaluación:Country / país Costa Rica City / Ciudad Fecha de compra:Location / lugar FRUIT / COSECHA Fruit shape / forma de la fruta Fresh weight / peso de la fruta (g) Fruit length / largo de la fruta (cm) Neck diameter / diámetro de cuello (cm) Neck circumference / circunferencia de cuello (cm) Bulb diameter / diámetro de bulbo (cm) Bulb circumfrence / circunferencia de bulbo (cm) Skin strength / dureza de la cáscara x4 (Kg) Skin texture / textura de la cáscara Skin color / color de la cáscara Secondary skin color / color de la cáscara secundario
Design of secondary skin color / diseño del color secundario de la fruta Rib form / forma de costilla Rind thickness / grosor de cáscara (mm) Flesh thickness / grosor de la pulpa (mm) Seed cavity diameter / diámetro de la cavidad (cm) % dry matter / % material seca x4 º Brix x3 Flesh color / color de la pulpa Flesh strength / dureza de la pulpa x4 SEED / SEMILLA Total count / numero de semillas total (¼ x4) Separation ease / facilidad de la seperacion Weight of 100 / peso de 100 (g) Coat texture / superficie de la semilla Coat luster / lustre de la superficie Coat color / color de la superficie Length / largo (mm) x5 Width / ancho (mm) x5 Thickness / grueso (mm) x5
Phenotypic Assessment
Genotypic Assessment(Make-up)
Observations and Outcomes
Alajuela Cartago Guanacaste Heredia Puntarenas San Jose0
2
4
6
8
10
12
14
Trait variation based on location
Brix% Dry matterColorMass (kg)
Location
Varia
ble
Alajuela Cartago Guanacaste Heredia Puntarenas San Jose0
2
4
6
8
10
12
14
BB B
AB
B
A
Dry matter variation based on location
Location
% d
ry m
atter
Observations and Outcomes
• Leela F1• Arjuna F1
Presence of hybrid varieties
Take Away
• Diversity present– Positive:
1. Use for breeding and improved varieties
2. Preserve biodiversity– Negative:
1. Yield inconsistency and possible explanation for increase hybrid varieties
Walter Barrantes
The Experience
‘Gringa de ayote’
Thank You• Andrew Ogden• Patricia Jarema• Brent Loy• Walter Barrantes• La familia Camacho• Georgeann Murphy • Peter Akerman• Hamel Center• Donors
– Mr. Hamel– Mr. & Mrs. Noonan– Mr. Carlton W. Allen
Questions NOITQ SEU S ?