sample cassette laser or led ccd camera or photo diode array display digital window sample holder...
Post on 19-Dec-2015
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sample cassette
Laser or LED
CCD camera or photo diode array
display
digital window sample holder
prismpolarizer lens
disposable sensor chip
cover plate
Compact SPR Biosensor
• For the sample with low concentration, signal shift might be smaller than environmental drift change.
• Self-referencing eliminates influence by environment (excellent robustness against temperature and buffer change).
substrate
PDMS flow cell
analyte
x
yz
Top view
Self-referencing SPR
Experimental procedure
glass slide
Ausubstrate
PDMS micro flow cell
Top view
Side view
width: 50 – 300 μm for channels and spaces between channels
Step 1
Step 2 Step 3
substrate
PDMS flow cell
x
y
z
channel width: 500 μm – 1 cm
glass slide
Au
solution
Multi-step functionalization procedure using mouse IgG as reference
glass slide
Au
sensing area
referencing area
MUA
antigen
mouse IgG
glass slide
AuS
OH
S
O
S S
OH
S
O
S S antibody
glass slide
AuS
OH
S
O
S S
OH
S
O
S S
glass slide
AuS
OH
S
O
S S
OH
S
O
S S
Taya, figs for pat appl, Aug 2005, fr Fengyu REV Aug 30.ppt
pbs
mouse IgG 100 g/ml
(a)
(b)
(c)
(d)
image pixel number
y ax
is o
f se
nsor
chi
pMouse IgG absorption onto the gold surface
(a)
(b)
(c)
(d)
image pixel number
y ax
is o
f se
nsor
chi
pAnti-CEA antibodies immobilized onto sensor chip
TKH2 20 g/ml STn 100 ng/ml
sensing (1)
referencing (2)
(1) - (2)
sensing (1)
referencing (2)
(1) - (2)
(a) (b)
Sensorgrams of TKH2 antibodies immobilizing onto sensor chip and STn antigens binding to TKH2 antibodies