sars-cov-2 antibody directory...a large collection of antibodies against different antigens of...
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THE COMPLETE GUIDE TO CHOOSING ANTIBODIES FOR COVID-19 RESEARCH
SARS-CoV-2 ANTIBODY DIRECTORY
Sino Biological Inc.
SARS-CoV-2 Antibody Buyer's Guide 01
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THE COMPLETE GUIDE TO SELECTING THE PROPER ANTIBODIES FOR YOUR COVID-19 STUDY
The ongoing COVID-19 pandemics has sickened 3.6
million people around the world, claiming over
250,000 lives. To address this global public health
crisis, unprecedented efforts are being made to
study the culprit virus, SARS-CoV-2. The biomedical
community are working to develop rapid diagnostic
reagents, vaccines, and therapies against this virus.
To support these studies, Sino Biological has developed
a large collection of antibodies against different
antigens of SARS-CoV-2. These antibodies can be
used to detect and/or neutralize the virus in a variety of
assays including ELISA, western blot, Immunofluorescent
staining, Immunohistochemistry, SPR (Biacore and
Octet), pseudovirus infection, and potentially in vivo
animal studies.
This guide book covers all available SARS-CoV-2 antibodies offered by Sino Biological. The readers can find
technical information about their specificity and sensitivity, cross-reactivity, neutralizing potential, and their
validated applications. The overarching goal for this guide is to help the readers to choose appropriate
antibodies for their own experiments.
Sino Biological Inc.
SARS-CoV-2 Antibody Buyer's Guide02
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SARS-CoV-2 ANTIGENS
S-Protein
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N-Protein
SARS-CoV-2
RBDS2
S1 ACE2
The spike protein (S-protein) is the common target for
neutralizing antibodies and vaccines. Spike protein
contains two subunits, S1 and S2. S1 contains a receptor
binding domain (RBD), which is responsible for recognizing
and binding with the cell surface receptor ACE2. S2 subunit
contains other basic elements needed for the membrane
fusion.
Same as all other coronaviruses, the genome of SARS-CoV-2 (2019-nCoV) encodes the spike protein, the envelope protein, the non-structure proteins including the proteases and methyltransferases, the membrane protein, and the nucleocapsid protein.
The Nucleocapsid Protein (N-protein) is a highly immunogenic phosphoprotein, and it is normally very conserved. The SARS-CoV-2 N protein is often used as a marker in diagnostic assays.
The methyltransferase is a non-structural protein. Methyltransferase mediates the methylation of the viral RNA, which may help the virus evade the host immune system. Methyltransferase inhibitor may work as an anti-viral treatment.
The papain-like protease (PlPro) is a protease. It mediates the post-translational processing of other non-structural proteins like replicase polyprotein. PlPro, and 3CLPro. PlPro is a target for antiviral drug development.
Angiotensin-converting enzyme 2 (ACE2) binds with the Spike protein of coronaviruses SARS-CoV and HCoV-NL63. This interaction is mediated by the RBD domain of the spike protein, and is believed to be the pivotal event in the membrane fusion process.
Sino Biological Inc.
SARS-CoV-2 Antibody Buyer's Guide 03
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NUCLEOCAPSID (N) PROTEIN ANTIBODIES
ELSIA Antibody PairsE
Pairs Ⅰ Pairs Ⅱ
Western Blot AntibodiesW
FULL LIST OF ANTI-NUCLEOCAPSID ANTIBODIES
2019-nCoV-N Concentration (pg/mL)
Opt
ical
Den
sity
(450
nm)
100
0.1
1
1000
Sample: Recombinant SARS-CoV-2 Nucleocapsid
KDa957255433426
30ng
10ng5ng
2ng 1ng
0.5ngCat # 40143-R019
Description Anti-Nucleocapsid
Type Rabbit MAb
Ig Type Rabbit IgG
Cat # 40143-R004 (Capture)
Description Anti-Nucleocapsid
Type Rabbit MAb
Ig Type Rabbit IgG
Cat # 40143-R040 (Detection)
Description Anti-Nucleocapsid
Type Rabbit MAb
Ig Type Rabbit IgG
Cat # 40143-MM05 (Capture)
Description Anti-Nucleocapsid
Type Mouse MAb
Ig Type Mouse IgG1
Cat # 40143-R001 (Detection)
Description Anti-Nucleocapsid
Type Rabbit MAb
Ig Type Rabbit IgG
Cat # Description Type Ig Type
40143-R001 Anti-Nucleocapsid Rabbit MAb Rabbit IgG
No cross-reactivity in ELISA with
Specific to SARS-CoV-2 Nucleocapsid
Specificity
MERS-CoV Nucleoprotein protein (40068-V08B)HCoV-229E Nucleoprotein protein (40640-V07E)HCoV-NL63 Nucleoprotein protein (40641-V07E)HCoV-HKU1(isolate N5) Nucleoprotein protein (40642-V07E)HCoV-OC43 Nucleoprotein (40643-V07E)
40143-R040 Anti-Nucleocapsid Rabbit MAb Rabbit IgG
40143-R004 Anti-Nucleocapsid Rabbit MAb Rabbit IgG
40588-R0004 Anti-Nucleocapsid Rabbit MAb Rabbit IgG
40143-R019 Anti-Nucleocapsid Rabbit MAb Rabbit IgG
40143-MM05 Anti-Nucleocapsid Mouse MAb Mouse IgG1
40143-MM08 Anti-Nucleocapsid Mouse MAb Mouse IgG1
40143-R040-H Anti-Nucleocapsid Rabbit MAb Rabbit IgG
40588-T62 Anti-Nucleocapsid Rabbit PAb Rabbit IgG
Sino Biological Inc.
SARS-CoV-2 Antibody Buyer's Guide04
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SPIKE PROTEIN ANTIBODIES
Pairs Ⅰ
Pairs Ⅱ
2019-nCoV-S1 Concentration (pg/mL)Opt
ical
Den
sity
(450
nm)
100
0.01
0.1
1
1000 10000
IF AntibodiesI
IF staining of SARS-CoV-2 Spike in ACE2-overexpressed 293T cells, infected by SARS-CoV-2-Spike pseudovirus (PSV).
1E-3 0.01 0.1 1 10 100
Conc.(nM)
Perc
ent A
ctiv
ity(%) 100
80
60
40
20
0
Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody was detected by SARS-CoV-2 Inhibitor Screening ELISA Kit (Catalog# KIT001). The IC50 is typically 0.857 nM.
Neutralization AntibodiesN
Antibodies against the S1 and RBD Domains
ELSIA Antibody PairsE
Cat # 40150-D003 (Capture)
Description Anti-Spike RBD
Type Chimeric MAb
Ig Type mouse v domain / human IgG1
Cat # 40150-D001 (Detection)
Description Anti-Spike RBD
Type Chimeric MAb
Ig Type mouse v domain / human IgG1
Cat # 40150-D002 (Capture)
Description Anti-Spike RBD
Type Chimeric MAb
Ig Type mouse v domain / human IgG1
Cat # 40150-D004 (Detection)
Description Anti-Spike RBD
Type Chimeric MAb
Ig Type mouse v domain / human IgG1
Cat # 40591-MM43
Description Anti-Spike S1
Type Mouse MAb
Ig Type Mouse IgG1
Cat # 40592-MM57
Description Anti-Spike RBD
Type Mouse MAb
Ig Type Mouse IgG2b
Cat # 40150-R007
Description Anti-Spike S1
Type Rabbit MAb
Ig Type Rabbit IgG
Sino Biological Inc.
SARS-CoV-2 Antibody Buyer's Guide 05
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SPIKE PROTEIN ANTIBODIES
FULL LIST OF ANTI-SPIKE ANTIBODIES
The binding between immobilized SARS-CoV-2 RBD protein and the anti-Spike antibody 40150-D005 in Octet RED assay
0 50 100 150 200 250 300 350 400 450 500 550 600
1.60
1.40
1.20
1.00
0.80
0.60
0.40
0.20
0.00
nm
Time(sec)
High Affinity Antibodies Antibodies against the S2 Protein
Cat # Description Type Ig Type
40591-MM43 Anti-Spike S1 Mouse Mab,Neutralizing Antibody Mouse IgG1
40150-R007 Anti-Spike S1 Rabbit MAb Rabbit IgG
40150-D001 Anti-Spike S1 Chimeric MAb mouse v domain / human IgG1
40150-D002 Anti-Spike S1 Chimeric MAb mouse v domain / human IgG1
40150-D003 Anti-Spike S1 Chimeric MAb mouse v domain / human IgG1
40150-D004 Anti-Spike S1 Chimeric MAb mouse v domain / human IgG1
40150-D005 Anti-Spike S1 Chimeric MAb mouse v domain / human IgG1
40150-D006 Anti-Spike S1 Chimeric MAb mouse v domain / human IgG1
40592-MM57 Anti-Spike RBD Mouse Mab,Neutralizing Antibody Mouse IgG2b
40592-T62 Anti-Spike RBD Rabbit PAb Rabbit IgG
40590-D001 Anti-Spike S2 Chimeric MAb mouse v domain / human IgG1
40590-T62 Anti-Spike S2 Rabbit PAb Rabbit IgG
40589-T62 Anti-Spike Rabbit PAb Rabbit IgG
Cat # 40150-D005
Description Anti-Spike RBD
Type Chimeric MAb
Ig Type mouse v domain / human IgG1
Cat # 40590-D001
Description Anti-Spike S2
Type Chimeric MAb
Ig Type mouse v domain / human IgG1
This is a chimeric antibody specifically recognizing the S2 domain of SARS-CoV-2. The specificity assay is assessed in ELISA which shows no cross-reactivity with SARS-CoV-2 S1, RBD, MERS-CoV S2 or Spike of common HCoVs (OC43, 229E, HKU1, NL63) .
Sino Biological Inc.
SARS-CoV-2 Antibody Buyer's Guide06
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CROSS-REACTIVITY AND SPECIFICITY
SARS-CoV-2 shares 80% sequence identity to the 2003 SARS-CoV. Hence, antibodies raised against SARS-CoV-2
antigens often shows various degree of cross-reactivity with the corresponding antigens of 2003 SARS-CoV. On the
other hand, the similarities between SARS-CoV-2 and other members of human coronavirus are very limited. The
following three clones of anti-spike antibodies have been tested against all seven coronavirus, and are shown to be
specific to SARS-CoV-2 spike.
The reactivity of the antibodies against the known coronavirus is assessed in ELISA. The reactivity (OD450) is illustrated in the figure below.
3.000
2.500
2.000
1.500
1.000
0.500
0.000
SARS
-CoV
-2
SARS
MERS
HCoV-H
KU1
HCoV-N
L63
HCoV-2
29E
HCoV-O
C43
OD
450
3.000
2.500
2.000
1.500
1.000
0.500
0.000
SARS
-CoV
-2
SARS
MERS
HCoV-H
KU1
HCoV-N
L63
HCoV-2
29E
HCoV-O
C43
OD
450
40592-MM57 40591-MM43
Cat # 40592-MM57
Description Spike RBD Antibody
Type Mouse MAb
Ig Type Mouse IgG2b
Application ELISA, Neutralization
Cat # 40591-MM43
Description Spike S1 Antibody
Type Mouse MAb
Ig Type Mouse IgG1
Application ELISA, Neutralization
Cat # 40592-R001
Description Anti-Spike RBD
Type Rabbit MAb
Ig Type Rabbit IgG
Application ELISA, Neutralization
Sino Biological Inc. Address: Building 9, No.18 Kechuang 10th St, BDABeijing, 100176, P.R.China Tel: +86-400-890-9989 Fax: +86-10-5095-3282 Website: www.sinobiological.com Email: [email protected]