scanning em of c. elegans
DESCRIPTION
Scanning em of C. elegans. Crawl movie. Fly-in movie. Male, scanning em. Male tail scanning em. Rene Mating Movie. Embryonic cell lineage. D lineage. ABala lineage. AIML lineage. AIML neuron. AIML circuit. Postembryonic blast cells. Z1Z2 Z3 Z4. Adult hermaphrodite anatomy. - PowerPoint PPT PresentationTRANSCRIPT
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Scanning em of C. elegans
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Crawl movie
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Fly-in movie
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Male, scanning em
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Male tail scanning em
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Rene Mating Movie
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Embryonic cell lineage
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D lineage
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ABala lineage
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AIML neuron
AIML lineage
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AIML circuit
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Z1Z2 Z3 Z4
Postembryonic blast cells
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Adult hermaphrodite anatomy
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C. elegans typical cross section
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Seam cell postembryonic lineages in the male
wild type
lin-4(e912) reiterations
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wild type
lin-14(sd) repeats S1
lin-14(lf) skips S1
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lin-4(e912) lin-14(sd)
lin-4(lf) and lin-14(gf) have the same phenotype: they repeat S1.
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LIN-4S is complementary to sequences in the 3’UTR of lin14.
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Human miRNA genes
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Symmetric neuron phenotype of lsy-6(-)
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A regulatory pathway leading to neuron asymmetry involving two micro RNA’s
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Worms Eating Bacteria Expressing dsRNA to a GFP Reporter Gene Activate RNAi
wild type worms RNAi-defective mutants
GFP lost in embryosGFP lost in the gut
from Mello and Conte,Nature 431, 338-342.
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RNAi in Plants
1928 report of developmentof viral (tobacco ringspot virus)resistance in tobacco, nowknown to be due to endogenousRNAi.
1928 report of developmentof viral (tobacco ringspot virus)resistance in tobacco, nowknown to be due to endogenousRNAi.
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RNAi/miRNA Pathways
from Meister and Tuschl, Nature431, 343-349, 2004.
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The Structure of Argonaute (Slicer)
Song et al., Science305, 1434-1437, 2004.