scap dbs a4-6page - prolab instruments gmbh · scap dbs system. according to the software-defined...
TRANSCRIPT
-
SCAP DBS System on a R80 platform with Stack 4 X 40
System DBS
Patent pending
Dried Blood Spot
Automated sampleextraction platformfor online LC-MS/MSbioanalysis
-
DBS Integrated Sample Processing
Automated sample processing Advantages
SCAP DBS system components mounted on a robotic platform
Comparison of conventional DBS sample handling with the SCAP DBS system
Dried blood spot (DBS) technology coupled to highly sensitive LC-MS/MS systems opens up new ways in bioanalytics both in the healthcare and the pharmaceutical industries. In the healthcare industry, complete measure-ments are done to diagnose patients to determine the efficacy of administered drugs or to screen for early detection of diseases. In the pharmaceutical industry, measurements are done during the development process of new drugs (e.g. PK/TK studies, etc.).
The major drawback of conventional DBS bio- analytics is the time consuming, error prone and labor intensive nature of manual sample pre-treatment. The SCAP DBS system from Prolab Instruments represents a unique approach for direct and automated DBS analysis and eliminates the drawback of current methods. The SCAP DBS system fully automates the DBS cards handling and allows online sample extraction without the need for punching of sample cards.
Fully automated DBS system sample card handling for LC/MS-MS analysis
Optimized extraction of DBS
Simplified method development by online extraction
Fully automated process saves time and labour costs
No need for additional consumables
Conventional DBS workflow:
Preparing logistics and consumables for offline DBS sample handling.
Load DBS card into SCAP DBS system and start automated process
SCAP DBS systemprocedure
Punching out DBS cards
Pipetting of extraction solvent(s) and internal standard
Extraction / Shaking – Ultra sonic treatment
Centrifugation / Filtration
Injection into HPLC - System
Injection into SCAP HPLC - System
-
Interchangable clamp adapters allow the extraction of variable area sizes from the DBS
SCAP DBS sample process
The rack with the DBS cards is placed into the SCAP DBS system. According to the software-defined sample list, the robotic arm transfers the card into the clamp module where the DBS card is directly inserted into the HPLC flow path for online extraction without punching out any part of the DBS card. The different adapters available for the SCAP DBS system allow for extraction of different areas of the sample spot and seal the DBS card against pressures of up to 200 bar.
The valve system configuration allows the online addition of internal standard (ISTD) during sample extraction. A SPE pre column is used for online sample cleanup prior to analytical separation on the main column.
Efficient SCAP DBS Workflow
Multiple spot extractionCamera for image analysis
SCAP DBS clamp adapters
Sample card returned to rack
Picks up sample card from SCAP DBS system rack
Places card into SCAP DBS system clamp module
DBS extraction
Phot clamp adapters
-
Efficient SCAP DBS Workflow
The robotic gripper tool picks up a sample card... ... and places it into the clamp module where it is inserted into the analytical flow path.
Dual valve application example
Column switching configuration with a six and a ten port valve
Clamp Adapters
Analytical Pump
ISTDLoop
WasteWaste
DBS Card
Dilutor ISTD
to MS
Analytical Column
Extraction Pump
21
34
5
6102
1
3
4
5 67
8
9
SPE Column Trap
A BA B
-
Software
Safety:Automatic detection of proper DBS card, card type, and sample spot
Precision:Automatic centration of sample spot for extraction targeting
Repeatability:Distinct offsets from spot center for up to four extractions of the same sample
Documentation:Complete tracking of samples from card to analytical data:
Card picture before and after extraction
Barcode reading (unique card ID and additional customer barcodes)
Run-time data (timestamps, barcodes, pictures) automatically attached to analytical data, allowing for integrated reports*
The comprehensive and easy-to-use software package includes:
Sample list
Method editor
Run control and detailed status feedback
History viewer
InterfacesExcel™ import and export for sample lists and run-time data
Analytical system (LC-MS/MS) connected by contact closure or software integration*
Flexible integration of customer devices
*depending on analytical data system used
Camera and Image Analysis
Offsets first spot extraction
Allows multiple extractions per spotDetects DBS and centers on the spot
Software
-
Heinig K, Wirz T, Bucheli F, Gajate-Perez A. Determination of oseltamivir (Tamiflu®) and oseltamivir carboxylate in dried blood spotsusing offline or online extraction. Bioanalysis. 3(4):421-37.
Ganz N, Singrasa M, Nicolas L, Gutierrez M, Dingeman-se J, Döbelin W,Glinski M. Development and validation of a fully automated online human dried blood spot analysis of bosentan and its metabolites using the Sample Card And Prep DBS System. J Chromatogr B Analyt Technol Biomed Life Sci. 15;885-886:50-60.
Prolab GmbH acknowledges all tradenames and trademarks used as the property of their respective owners.
SCAP system® is a registered trademark of Prolab Instruments GmbH, Patent Pending
Camera Card Stack Gripper Clamp Module Valve Module ISTD Dilutor
Kägenstrasse 17CH-4153 ReinachSwitzerland
Tel: +41 61 712 01 28Fax: +41 61 712 01 48Web: www.prolab.che-mail: [email protected]
SCAP DBS sales contact:
Literature