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ACROSS THE HALL OR ACROSS THE GLOBE, REFMAN KEEPS EVERYBODY ON THE SAME PAGE. © Copyright 2008 Thomson Reuters. Reference Manager is a registered trademark of Thomson Reuters. All trademarks are the property of their respective companies. No matter where you or your colleagues are in the world, so are your references. With Reference Manager, ® you can collaborate with others on a network—or even the Web. Simplify your publishing with Reference Manager where you can search Internet databases, organize your references and create instant bibliographies. Plus, with newly added features you can do even more. Simplify your life and keep everybody on the same page today. 800-722-1227 760-438-5526 [email protected] Download a free trial www.refman.com 12 Attach any file to a record and create a single Reference Manager repository Cite While You Write with a Reference Manager tab on the Word 2007 ribbon Use Web Publisher to collaborate with colleagues

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Page 1: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

ACROSS THE HALL OR ACROSS THE GLOBE,

REFMAN KEEPS EVERYBODY ON THE SAME PAGE.

© Copyright 2008 Thomson Reuters. Reference Manager is a registered trademark of Thomson Reuters.

All trademarks are the property of their respective companies.

Nomatter where you or your colleagues are in the world, so are your

references. With Reference Manager,® you can collaborate with

others on a network—or even the Web. Simplify your publishing

with Reference Manager where you can search Internet databases,

organize your references and create instant bibliographies. Plus, with

newly added features you can do even more.

Simplify your life and keep everybody on the same page today.

800-722-1227 • 760-438-5526 • [email protected]

Download a free trial

www.refman.com

12• Attach any file to a record and create a

single Reference Manager repository

• Cite While You Write™ with a Reference

Manager tab on the Word 2007 ribbon

• Use Web Publisher to collaborate with colleagues

Page 2: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

askbigquestions

g

• helping answer big questionsin drug delivery

Bold progress occurs when analytical technologies and lab products keep pace with

the big questions that come up every day. In the lab, every new answer leads to another

question. Questions we help scientists and engineers tackle. From mass specs to

consumables to protein reagents, we deliver the innovative instruments and equipment

to help optimize application and industry solutions. Driving bold progress through routine

days and eureka discoveries. Question after question. Go ahead. Ask.

• see the full breadth of bold progress at thermoscientific.com

©2010

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Page 3: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

GE HealthcareLife Sciences

It’s all the convenience you want in a spectrophotometer, packaged in a portable,

ergonomic device. NanoVue Plus features a new hydrophobic, gold-colored sample

plate coating that delivers outstanding results for sub-microliter amounts of proteins

and nucleic acids. It operates without a PC and does not require time-consuming third-

party path length recalibration.

NanoVue Plus enables easy protocol selection using advanced software that includes

intuitive drop-down lists for the full range of CyDye™ fluorescent dyes, as well as lists

for common fluors. Results can exported using a USB cable or Bluetooth™ connections

for print via computer (PVC) or stored using the new SD card option. An integrated

printer is also available.

• Swift, accurate analysis of 0.5 µl samples

of nucleic acids and proteins

• Practical drop and measure mechanism

• Outstanding sample recovery

• Reliable and reproducible measurements

• Automatic self-calibration on start up

• Path length recalibration kit available

as accessory

Experience the NanoVue Plus first hand.

Register for a trial at:

www.gelifesciences.com/tryNanoVuePlus

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Drop. Measure. Done.NanoVue™ Plus: intelligent performanceacross all spectrophotometer applications

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Page 4: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

Sample & Assay Technologies

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Automated solutions from sample to result:

■ The widest choice of sample processing protocols

■ Low-, medium-, and high-throughput automation

■ Leading solutions for molecular diagnostics

■ Plug-and-play automated sample preparation

■ Quantitative, real-time PCR detection

■ Automated analysis of DNA fragments and RNA

■ High-resolution sequence-based DNA detection and quantification

■ HPV testing

Making improvements in life possible — www.qiagen.com

Automated sample and assay technologies

by QIAGEN

Free up your time

Page 5: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

Moving science forward

Emotional reactions to instrumentation from scientists are rare.

Yet with Thermo Scientific NanoDrop Spectrophotometers, they

are becoming commonplace. That’s because scientists who

own a NanoDrop™ are passionate about its simplicity. These

instruments reduce analysis time and minimize sample waste

with fast, easy and accurate micro-volume nucleic acid and

protein sample quantitation. Just ask Dr. Lee:

“With NanoDrop I use less sample. Reproducibility of results

is much higher—and I get them in a few seconds. The previous

machine I used to measure DNA took much longer, and the

results weren’t nearly as reliable. NanoDrop is much faster. It

is small, quick and convenient.”

“NanoDrop is quick and gives you reproducible results every time.”

Try any NanoDrop instrument for free:* www.thermo.com/nanodrop

“I really like this instrument! Reliability and reproducibility areso important.” —Hing Cheong (Henry) Lee, Ph.D.

Thermo Scientific NanoDrop UV-Vis

Spectrophotometers offer easy, reliable

micro-volume analysis, with sample size as

low as 0.5 µl and measurement time of less

than 5 seconds—no dilutions.

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Page 6: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

REMARKABLY FAST

www.neb.com

CLONING & MAPPINGDNA AMPLIFICATION

& PCRRNA ANALYSIS

PROTEIN EXPRESSION

& ANALYSIS

GENE EXPRESSION

& CELLULAR ANALYSIS

Not all PCR polymerases are created equal

Amplification of a 3.8 kb fragment from the human beta globin gene clearly illustrates the

extreme speed and robustness offered by using Phusion DNA Polymerase. Reactions were

performed according to the suppliers’ recommendations using varying extension times

(shown above gel).

Phusion is a registered trademark of Finnzymes Oy.

Phusion® High-Fidelity DNA PolymeraseWith Phusion® High-Fidelity DNA Polymerase, there is no need to compromise any aspect of your PCR performance.

A superior choice for cloning, this recombinant polymerase has an error rate 50-fold lower than Taq DNA Polymerase,

combining extreme precision with unparalleled speed and robustness.

Advantages:• Extreme fidelity

• High speed

• Robustness

Distributed byProduced by

Go to www.neb.com/phusion to find out

how Phusion High-Fidelity DNA Polymerase can

improve your PCR performance.

• High yield

• Specificity

Page 7: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

Roche NimbleGen, Inc.

Madison, WI USAFor life science research only. Not for use in diagnostic procedures.NIMBLEGEN is a trademark of Roche

© 2010 Roche NimbleGen, Inc. All rights reserved.

Discover next-generation genomics at www.nimblegen.com

Taking Your Research to the Next-Level...

Next-Generation Genomics from Roche NimbleGen

The future of genomics is available today with tools flexible enough to conform to

your specific research needs. Tools that let you discover your data, your way, and

provide you with the most complete picture of your disease-associated research.

Only Roche NimbleGen provides you with the genomic content, high data quality,

and array format flexibility you need to make next-generation genomics a reality.

Sequence Capture • CGH/CNV • ChIP-chip • DNA Methylation • Gene Expression

Page 8: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

BD Flow Cytometry Systems

Originally developed to support marine biologists, the Small ParticleDetector improves forward scatter detection, revealing particles downto 200 nm. Today, this BD Influx™ flow cytometry innovation empowersinvestigations in bacteriology, microbiology, and environmental sciences.

At BD, we keep pushing the edge of what advanced flow cytometry can do,so that new avenues of investigation and discovery continually open for you.To us, innovation isn’t a destination, it’s the way forward.

Innovation. To be continued.

BD flow cytometers are Class I (1) laser products.For Research Use Only. Not for use in diagnostic or therapeutic procedures.BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2010 BD23-11741-00

bdbiosciences.com/innovation

Small Particle Detectorwith polarization

BD Biosciences2350 Qume DriveSan Jose, CA 95131bdbiosciences.com

AAAS is here –

increasing diversity in the

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AAAS is working to ensure that every student

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member your dues support these efforts.

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Together we can make a difference.

To learn more,

visit aaas.org/plusyou/entrypoint

Page 9: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

Deadline for entries

June 15, 2010

It’s easy to apply! Learn more at

www.eppendorf.com/prize

Ku

rstin

Ro

eP

ho

tog

rap

hy

Congratulations to Dr. Richard Benton on winning the 2009

Eppendorf & Science Prize for his studies on odor detection in

the fruit fly, Drosophila. His findings have revealed unexpected

evolutionary parallels between insect chemosensation, immune

recognition and synaptic transmission.

The annual international Eppendorf & Science Prize for

Neurobiology honors young scientists for their outstanding

contributions to neurobiology research based on methods of

molecular and cell biology. The winner and finalists are selected

by a committee of independent scientists, chaired by Science’s

Senior Editor, Dr. Peter Stern.

To be eligible, you must be 35 years of age or younger. If you’re

selected as this year’s winner, you will receive US$ 25,000, have

your work published in the prestigious journal Science and be

invited to visit Eppendorf in Hamburg, Germany.

Get recognized!

US$ 25,000

Prize

2009 Winner

Richard Benton, Ph.D.

Assistant Professor

University of Lausanne

Switzerland

Page 10: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

Drug Discovery & Development Week

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Save up to $479 o� the standard rate –use priority code: D10201SCI

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Page 11: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

New Products, Services, and Solutions

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Producedbyan international teamofscience journalists,ScienceInsider offers hard-hitting coverage on a rangeofissues including climate change, bioterrorism, researchfunding, andmore.

Before research happens at the bench, science policy isformulated in the halls of government. Make sure youunderstandhowcurrent eventsare impactingyourwork.Read ScienceInsider today.

Breaking news and analysis from

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Page 12: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

BD Flow Cytometry Systems

A unique fluidics design in the BD Influx™ cell sorter allows the entire sheath and

sample path, from tank through nozzle, to be easily replaced using a rapid fluidics

replacement kit. Disposable fluidics eliminates cross contamination from previous

sorts to empower new frontiers of research such as next generation cell therapies.

Innovation that inspires innovation. It’s this kind of forward thinking that you can

depend on from BD to keep your lab in a perpetual state of the art.

Innovation. To be continued.

Disposable fluidics kit

BD Biosciences2350 Qume DriveSan Jose, CA 95131bdbiosciences.com

BD flow cytometers are Class I (1) laser products.For Research Use Only. Not for use in diagnostic or therapeutic procedures.BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2010 BD23-11742-00

bdbiosciences.com/innovation

Page 13: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

BD Biosciences flow cytometry systems

In a perpetual state of the art.

BD Biosciences2350 Qume DriveSan Jose, CA 95131bdbiosciences.com

Class I (1) Laser Product.

BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2010 BD

23-11480-01

At BD Biosciences, we’re always thinking about

what’s next in flow cytometry and multicolor

analysis. Since commercializing the first cell

sorter in 1973, we’ve been delivering flow

cytometry systems that are more powerful,

more dependable, and easier to use.

We innovate to keep you in a perpetual state

of the art, because when we do, your work

goes more smoothly, clearing the way to faster

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From special order systems that support

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to make your job easier, your workflow more

efficient, and your results more reliable, today

and tomorrow.

Innovation is built in.You can depend on it.

bdbiosciences.com/innovation

Page 14: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

10 Ways to ImproveYour Chances of Securing Research Funding

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There are many approaches to securing funding, but not all are effective.

That’s why the American Association for the Advancement of Science is committed to

offering its members a variety of resources to help them locate the money they need—–

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of funding opportunities. Join us. Together we

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Page 15: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

The Power of MORE

Applied Biosystems, the most trusted name in PCR instruments,

introduces a new line of innovative thermal cyclers.

The Veriti® 96-Well Thermal Cycler delivers the proven reliability of the GeneAmp® PCR

Systems 9600 and 9700 instruments. With the added control of VeriFlex™ Blocks, you have

six independent temperature blocks enabling precise control over your PCR optimization.

Additionally, the powerful user interface through the color touch screen helps simplify the

setup and use of the Veriti® 96-Well Thermal Cycler. Optional setups for fast or standard PCR

methods allow for the flexibility to shorten your PCR cycling time.

To learn more about the Veriti® Thermal Cycler or to view the product video,

visit info.appliedbiosystems.com/veriti.

For research use only. Not for human or animal diagnostic or therapeutic use.

NOTICE TO PURCHASER:

The Veriti® Thermal Cyclers are covered by one or more of US Patents Nos. 5,475,610, 5,552,580, 5,602,756, 6,703,236, 7,238,517, 7,504,241, and corresponding claims in their non-US counterparts, owned by Life Technologies Corporation. No right is conveyed expressly, by

implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5’ nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850

Lincoln Centre Drive, Foster City, California 94404, USA.

© 2010 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.

The Veriti® Thermal Cycler

Page 16: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

1179www.sciencemag.org/products SCIENCE VOL 328 28 MAY 2010

NEW PRODUCTS

LIFE SCIENCE TECHNOLOGIES

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are

featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or

materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.

AAAS/Science Business Office

PULSELESS LIQUID FLOWThe Mitos P-pump provides a pulseless liquid flow via a precise

pressure-driven pumping mechanism. With its excellent response

time and accuracy, the pressure-driven flow is suitable for microflu-

idic systems in which stable flow is required for applications such as

droplet formation. Operating over a wide pressure range of 0–10 bar,

the pump is suitable for use with systems of high fluidic resistance.

The design features a lockable pressure chamber for safety, which is

easily accessible and accommodates a wide range of standard and

nonstandard fluid vessels. With the ability to contain samples from

100 ml to 30 ml, the pump uses fluid directly from the container,

producing no mess or waste. The pump can work with a laboratory

nitrogen or argon supply, gas bottle, or compressor.

Dolomite

For info: +44-1763-242491 www.dolomite-microfluidics.com

EXTENDED RANGE PROTEIN STANDARDA new addition to the Precision Plus Protein line of protein stan-

dards, the Precision Plus Protein Dual Xtra standard provides 12

reference bands, from 2 kD to 250 kD. The new standard allows

researchers to perform electrophoresis experiments with proteins

of varying sizes, without the need to use two lanes in a gel to load

two different markers for measurement of proteins in the high and

low molecular weight range. The extended range of the new stan-

dard allows researchers to use just one molecular weight standard,

whether they need to assess proteins with a wide molecular weight

range or to focus on the low range only. The new dual standard of-

fers the same sharp, bright, evenly spaced bands as the rest of the

Precision Plus Protein family of standards.

Bio-Rad

For info: 800-424-6723 www.bio-rad.com

OPTICAL OXYGEN SENSORThe NeoFox Sport is a portable, handheld optical oxygen sensor for

measuring dissolved and gaseous oxygen pressure in a variety of

media. The sensor makes use of a proprietary sol-gel coating that is

embedded with an oxygen indicator and can be applied to patches or

probes. Patches are useful for measuring headspace gases and oth-

er parameters inside packages and containers. Probe options range

from slender, 300-mm–diameter fiber assemblies for fine spatial res-

olution applications to robust, quarter-inch stainless steel configura-

tions for process environments. Coating formulations are available

for general lab use, high-sensitivity applications, and hydrocarbon-

rich sample environments. The NeoFox Sport fluorescence-based

optical oxygen system includes an onboard microprocessor with a

low-power organic LED display, a user interface, and a battery pack.

Ocean Optics

For info: 727-733-2447 www.OceanOptics.com

MICROARRAYThe HumanOmniExpress BeadChip is part of the Omni family of

microarrays for genomewide association studies. Designed in a

12-sample format, the OmniExpress allows interrogation of more

than 700,000 variants per sample, for more than 8 million data points

on a single chip. When used with the Illumina iScan System, Omni-

Express allows processing of more than 1,400 samples per week.

The markers on the OmniExpress incorporate an optimized set of

single nucleotide polymorphisms chosen from all three phases of

the International HapMap Project.

Illumina

For info: 858-882-6822 www.illumina.com/OmniExpress

PROTEIN A KIT AND IMMOBILIZATION STATIONThe Dip and Read Residual Protein A Detection Kit is for use on

ForteBio’s Octet platform.The new assay enables drug developers to

measure levels of Protein A quickly with minimal hands-on time. The

kit consists of biosensors and all the reagents required to quantitate

Protein A or biosimilars in the presence of human immunoglobulin

G. Another addition to the Octet platform, the Sidekick Offline Bio-

sensor Immobilization Station, enables offline loading of reagents

on biosensors in tandem with any Octet instrument.The station pro-

vides simultaneous and uniform sample loading on all 96 biosensors

on a biosensor tray and shakes the samples while keeping the bio-

sensors immobile, which decreases the incubation time by half. This

process significantly increases the sensitivity of sandwich assays.

Fortebio

For info: 650-289-6800 www.fortebio.com

SMALL-CAPACITY INCUBATORS

The Midi 40 is a small-capacity carbon dioxide incubator that delivers the reliable

culturing performance of a full-sized incubator in a convenient, space-saving footprint.

The Midi 40 is suitable for researchers who lack space or require separate, undisturbed

incubation conditions due to the contamination risk associated with shared incubators.

Additional benefits include reduced energy and gas consumption as well as lower heat

output. The incubation parameters can be easily managed through the incubator’s

bright, vacuum fluorescent display and easy-to-navigate membrane keypad. The unit

features a noncorrosive, stainless steel chamber with 40 liters of culture space, and a

heated humidity pan to generate relative humidity values of up to 95 percent.

Thermo Fisher Scientific

For info: 508-742-5254 www.thermo.com/incubators

Page 17: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

Introducing the new BD InfluxChoice. Flexibility. Control. The BD Influx™ cell

sorter offers boundless adaptability to application

and environmental requirements without

sacrificing speed or accuracy. Available with up

to 7 lasers, up to 6-way sorting, and a range of

detection configurations, the instrument offers a

combination of powerful technology and precision

controls for flow cytometry as exacting as your

research demands.

Innovative thinking is evident throughout the design

of the new BD Influx, with a modular component

architecture and hands-on controls that make it

ready to adapt to your unique requirements.

In addition, the new BD Influx software puts

hundreds of parameters under your control

with rich analysis capabilities to let you visualize

experimental data in a meaningful way—from

histograms to contour plots.

Find out how the innovation built in to the

BD Influx can keep your lab in a perpetual state

of the art at bdbiosciences.com/influx.

Innovation is built in.You can depend on it.

BD Biosciences

2350 Qume DriveSan Jose, CA 95131bdbiosciences.com

Class I (1) Laser Product.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2010 BD

23-11743-00

Introducing the New

BD Influx Cell Sorter

bdbiosciences.com/influx

Page 18: Science Magazine · GE Healthcare Life Sciences It’s all the convenience you want in a spectrophotometer, packaged in a portable, ... At BD, we keep pushing the edge of what advanced

For research use only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.2010 © Life Technologies. All rights reserved. These products may be covered by one or more Limited Use Label Licenses (details of the LULLs can be found at www.invitrogen.com). CO12739 0410

www.invitrogen.com

Put the odds in your favorwith SuperScript® RT.

Why chance your research with anythingother than the most reliable, consistentreverse transcriptase in the industry?Choose SuperScript®III RT for robust first-strand synthesis and great

cDNA yields. Engineered to be RNase H minus and thermostable up to

55°C, SuperScript®III RT delivers best-in-class cDNA synthesis in every

single reaction, combining maximum yield with maximum sensitivity.

You get full-length cDNA without degrading rare RNA transcripts. And

whether you’re cloning, doing micro¬array analysis, or monitoring gene

expression with qRT-PCR, SuperScript®III RT always delivers the results

you need to move forward—the first time, every time. You can bet on it.

Go for complete reliability at www.invitrogen.com/superscript.

Engineered to be RNase H minus and

incredibly thermostable, SuperScript®III RT

delivers robust �rst-strand synthesis with

both great cDNA yield and ultimate sensitiv-

ity, whatever the size of your mRNA.